Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
Authorized Users Only
2013
Authors
Ignjatović, Nenad
Ajduković, Zorica

Savić, Vojin
Najman, Stevo

Mihailović, Dragan
Vasiljević, Perica

Stojanović, Zoran S.

Uskoković, Vuk

Uskoković, Dragan

Article (Published version)

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Show full item recordAbstract
Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alv...eolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
Keywords:
cobalt-substituted hydroxyapatite / bone regeneration / nanoparticles / osteoporosisSource:
Journal of Materials Science: Materials in Medicine, 2013, 24, 2, 343-354Publisher:
- Springer
Funding / projects:
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
- United States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
- United States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
DOI: 10.1007/s10856-012-4793-1
ISSN: 0957-4530
PubMed: 23090835
WoS: 000314775100008
Scopus: 2-s2.0-84878374725
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ignjatović, Nenad AU - Ajduković, Zorica AU - Savić, Vojin AU - Najman, Stevo AU - Mihailović, Dragan AU - Vasiljević, Perica AU - Stojanović, Zoran S. AU - Uskoković, Vuk AU - Uskoković, Dragan PY - 2013 UR - https://dais.sanu.ac.rs/123456789/353 AB - Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study. PB - Springer T2 - Journal of Materials Science: Materials in Medicine T1 - Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones SP - 343 EP - 354 VL - 24 IS - 2 DO - 10.1007/s10856-012-4793-1 UR - https://hdl.handle.net/21.15107/rcub_dais_353 ER -
@article{ author = "Ignjatović, Nenad and Ajduković, Zorica and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Stojanović, Zoran S. and Uskoković, Vuk and Uskoković, Dragan", year = "2013", abstract = "Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.", publisher = "Springer", journal = "Journal of Materials Science: Materials in Medicine", title = "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones", pages = "343-354", volume = "24", number = "2", doi = "10.1007/s10856-012-4793-1", url = "https://hdl.handle.net/21.15107/rcub_dais_353" }
Ignjatović, N., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., Stojanović, Z. S., Uskoković, V.,& Uskoković, D.. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine Springer., 24(2), 343-354. https://doi.org/10.1007/s10856-012-4793-1 https://hdl.handle.net/21.15107/rcub_dais_353
Ignjatović N, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović ZS, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine. 2013;24(2):343-354. doi:10.1007/s10856-012-4793-1 https://hdl.handle.net/21.15107/rcub_dais_353 .
Ignjatović, Nenad, Ajduković, Zorica, Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Vasiljević, Perica, Stojanović, Zoran S., Uskoković, Vuk, Uskoković, Dragan, "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones" in Journal of Materials Science: Materials in Medicine, 24, no. 2 (2013):343-354, https://doi.org/10.1007/s10856-012-4793-1 ., https://hdl.handle.net/21.15107/rcub_dais_353 .