Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize
Authors
Gvozdenović, Milica M.
Jugović, Branimir

Bezbradica, Dejan

Antov, Mirjana

Knežević Jugović, Zorica

Grgur, Branimir

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Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.
Keywords:
glucose oxidase / polyaniline / immobilization / electrochemical synthesisSource:
The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts, 2010, s11-P-041Publisher:
- Nice : International Society of Electrochemistry
Funding / projects:
- Modifikacija metalnih i nemetalnih materijala elektroprovodnim polimerima za primenu u novim tehnologijama (RS-142044)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Gvozdenović, Milica M. AU - Jugović, Branimir AU - Bezbradica, Dejan AU - Antov, Mirjana AU - Knežević Jugović, Zorica AU - Grgur, Branimir PY - 2010 UR - https://dais.sanu.ac.rs/123456789/3512 AB - Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme. PB - Nice : International Society of Electrochemistry C3 - The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize VL - s11-P-041 UR - https://hdl.handle.net/21.15107/rcub_dais_3512 ER -
@conference{ author = "Gvozdenović, Milica M. and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević Jugović, Zorica and Grgur, Branimir", year = "2010", abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.", publisher = "Nice : International Society of Electrochemistry", journal = "The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts", title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize", volume = "s11-P-041", url = "https://hdl.handle.net/21.15107/rcub_dais_3512" }
Gvozdenović, M. M., Jugović, B., Bezbradica, D., Antov, M., Knežević Jugović, Z.,& Grgur, B.. (2010). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts Nice : International Society of Electrochemistry., s11-P-041. https://hdl.handle.net/21.15107/rcub_dais_3512
Gvozdenović MM, Jugović B, Bezbradica D, Antov M, Knežević Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts. 2010;s11-P-041. https://hdl.handle.net/21.15107/rcub_dais_3512 .
Gvozdenović, Milica M., Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize" in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts, s11-P-041 (2010), https://hdl.handle.net/21.15107/rcub_dais_3512 .