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Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize

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2010
bitstream_11189.pdf (212.5Kb)
Authors
Gvozdenović, Milica M.
Jugović, Branimir
Bezbradica, Dejan
Antov, Mirjana
Knežević Jugović, Zorica
Grgur, Branimir
Conference object (Published version)
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Abstract
Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.
Keywords:
glucose oxidase / polyaniline / immobilization / electrochemical synthesis
Source:
The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts, 2010, s11-P-041
Publisher:
  • Nice : International Society of Electrochemistry
Funding / projects:
  • Modifikacija metalnih i nemetalnih materijala elektroprovodnim polimerima za primenu u novim tehnologijama (RS-142044)
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_dais_3512
URI
https://dais.sanu.ac.rs/123456789/3512
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - CONF
AU  - Gvozdenović, Milica M.
AU  - Jugović, Branimir
AU  - Bezbradica, Dejan
AU  - Antov, Mirjana
AU  - Knežević Jugović, Zorica
AU  - Grgur, Branimir
PY  - 2010
UR  - https://dais.sanu.ac.rs/123456789/3512
AB  - Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.
PB  - Nice : International Society of Electrochemistry
C3  - The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts
T1  - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize
VL  - s11-P-041
UR  - https://hdl.handle.net/21.15107/rcub_dais_3512
ER  - 
@conference{
author = "Gvozdenović, Milica M. and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević Jugović, Zorica and Grgur, Branimir",
year = "2010",
abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.",
publisher = "Nice : International Society of Electrochemistry",
journal = "The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts",
title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize",
volume = "s11-P-041",
url = "https://hdl.handle.net/21.15107/rcub_dais_3512"
}
Gvozdenović, M. M., Jugović, B., Bezbradica, D., Antov, M., Knežević Jugović, Z.,& Grgur, B.. (2010). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts
Nice : International Society of Electrochemistry., s11-P-041.
https://hdl.handle.net/21.15107/rcub_dais_3512
Gvozdenović MM, Jugović B, Bezbradica D, Antov M, Knežević Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts. 2010;s11-P-041.
https://hdl.handle.net/21.15107/rcub_dais_3512 .
Gvozdenović, Milica M., Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize" in The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts, s11-P-041 (2010),
https://hdl.handle.net/21.15107/rcub_dais_3512 .

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