Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.
The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts, 2010, s11-P-041
TY - CONF
AU - Gvozdenović, Milica M.
AU - Jugović, Branimir
AU - Bezbradica, Dejan
AU - Antov, Mirjana
AU - Knežević Jugović, Zorica
AU - Grgur, Branimir
PY - 2010
UR - http://dais.sanu.ac.rs/123456789/3512
AB - Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.
PB - Nice : International Society of Electrochemistry
C3 - The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts
T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize
VL - s11-P-041
ER -
@conference{
author = "Gvozdenović, Milica M. and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević Jugović, Zorica and Grgur, Branimir",
year = "2010",
url = "http://dais.sanu.ac.rs/123456789/3512",
abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilization of Glucose oxidase (GOx) via glutaraldehyde into electrochemicaly polymerized PANI on graphite electrode. Electrochemical polymerization of PANI on graphite was performed from aqueous solution of 1.0 mol dm-3 HCl and 0.25 mol dm-3 aniline at constant current density of 2.0 mA cm-2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm-2 were recorded in different glucose concentrations. The linearity response range was between 1.0-5.0 mmol dm-3 of glucose concentration. The estimated apparent Michaelis-Menten constant, was Km ′ = 0.30 mmol dm-3, which is scientifically lower than that of free enzyme.",
publisher = "Nice : International Society of Electrochemistry",
journal = "The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts",
title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize",
volume = "s11-P-041"
}
Gvozdenović MM, Jugović B, Bezbradica D, Antov M, Knežević Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize. The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of Abstracts. 2010;s11-P-041
Gvozdenović, M. M., Jugović, B., Bezbradica, D., Antov, M., Knežević Jugović, Z.,& Grgur, B. (2010). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize.
The 61st Annual Meeting of the International Society of Electrochemistry: Electrochemistry from Biology to Physics September 26th - October 1st, 2010, Nice, France: Book of AbstractsNice : International Society of Electrochemistry., s11-P-041.
Gvozdenović Milica M., Jugović Branimir, Bezbradica Dejan, Antov Mirjana, Knežević Jugović Zorica, Grgur Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidize" s11-P-041 (2010)
