Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry
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Clindamycin was determined in pig plasma by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The multiple reaction monitoring (MRM) mode of precursor-product ion transitions for clindamycin (m/z=421.1/126.1) and the internal standard, caffeine (m/z=192/125) was used. The samples were prepared by two methods: 0.1% formic acid in methanol and 1.5% trichloacetic acid. The recovery for the two preparation methods at 0.05mg/ml (n=6) was found to be for the first 104.3% and for the second method 106.5%, with repeatability RSD 1.1% and RSD 4.34%, respectively. The results of the comparison of the two different preparation methods of samples demonstrated that both methods were satisfactory.
Keywords:LC-MS/MS / clindamycin / poly(D,L-lactide-co-glycolide) / hydroxyapatite / core-shell nanoparticles
Source:Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia, 2010, 19-19
- Belgrade : Institute of Technical Sciences of SASA