Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry
Authors
Vuković, GoricaLazić, Sanja
Šunjka, Dragana
Bursić, Vojislava
Šarčev, Ivan
Vukomanović, Marija

Ignjatović, Nenad

Uskoković, Dragan

Conference object (Published version)
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Show full item recordAbstract
Clindamycin was determined in pig plasma by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The multiple reaction monitoring (MRM) mode of precursor-product ion transitions for clindamycin (m/z=421.1/126.1) and the internal standard, caffeine (m/z=192/125) was used. The samples were prepared by two methods: 0.1% formic acid in methanol and 1.5% trichloacetic acid. The recovery for the two preparation methods at 0.05mg/ml (n=6) was found to be for the first 104.3% and for the second method 106.5%, with repeatability RSD 1.1% and RSD 4.34%, respectively. The results of the comparison of the two different preparation methods of samples demonstrated that both methods were satisfactory.
Keywords:
LC-MS/MS / clindamycin / poly(D,L-lactide-co-glycolide) / hydroxyapatite / core-shell nanoparticlesSource:
Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia, 2010, 19-19Publisher:
- Belgrade : Institute of Technical Sciences of SASA
Funding / projects:
- Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou (RS-142006)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Vuković, Gorica AU - Lazić, Sanja AU - Šunjka, Dragana AU - Bursić, Vojislava AU - Šarčev, Ivan AU - Vukomanović, Marija AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2010 UR - https://dais.sanu.ac.rs/123456789/3453 AB - Clindamycin was determined in pig plasma by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The multiple reaction monitoring (MRM) mode of precursor-product ion transitions for clindamycin (m/z=421.1/126.1) and the internal standard, caffeine (m/z=192/125) was used. The samples were prepared by two methods: 0.1% formic acid in methanol and 1.5% trichloacetic acid. The recovery for the two preparation methods at 0.05mg/ml (n=6) was found to be for the first 104.3% and for the second method 106.5%, with repeatability RSD 1.1% and RSD 4.34%, respectively. The results of the comparison of the two different preparation methods of samples demonstrated that both methods were satisfactory. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia T1 - Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry SP - 19 EP - 19 UR - https://hdl.handle.net/21.15107/rcub_dais_3453 ER -
@conference{ author = "Vuković, Gorica and Lazić, Sanja and Šunjka, Dragana and Bursić, Vojislava and Šarčev, Ivan and Vukomanović, Marija and Ignjatović, Nenad and Uskoković, Dragan", year = "2010", abstract = "Clindamycin was determined in pig plasma by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The multiple reaction monitoring (MRM) mode of precursor-product ion transitions for clindamycin (m/z=421.1/126.1) and the internal standard, caffeine (m/z=192/125) was used. The samples were prepared by two methods: 0.1% formic acid in methanol and 1.5% trichloacetic acid. The recovery for the two preparation methods at 0.05mg/ml (n=6) was found to be for the first 104.3% and for the second method 106.5%, with repeatability RSD 1.1% and RSD 4.34%, respectively. The results of the comparison of the two different preparation methods of samples demonstrated that both methods were satisfactory.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia", title = "Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry", pages = "19-19", url = "https://hdl.handle.net/21.15107/rcub_dais_3453" }
Vuković, G., Lazić, S., Šunjka, D., Bursić, V., Šarčev, I., Vukomanović, M., Ignjatović, N.,& Uskoković, D.. (2010). Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry. in Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia Belgrade : Institute of Technical Sciences of SASA., 19-19. https://hdl.handle.net/21.15107/rcub_dais_3453
Vuković G, Lazić S, Šunjka D, Bursić V, Šarčev I, Vukomanović M, Ignjatović N, Uskoković D. Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry. in Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia. 2010;:19-19. https://hdl.handle.net/21.15107/rcub_dais_3453 .
Vuković, Gorica, Lazić, Sanja, Šunjka, Dragana, Bursić, Vojislava, Šarčev, Ivan, Vukomanović, Marija, Ignjatović, Nenad, Uskoković, Dragan, "Determination of clindamycin in pig plasma after implantation of poly(D,Llactide-co-glycolide)/hydroxyapatite/clindamycin core–shell nanosphere by liquid chromatography-tandem mass spectrometry" in Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia (2010):19-19, https://hdl.handle.net/21.15107/rcub_dais_3453 .