Investigation of HeNe laser therapy influence on BCP/PLGA osseointegration: Experimental study

Abstract

The efficiency of HeNe laser application in treatment of artificially caused bone defects in mandible of rats filled with biocomposite BCP/PLGA (bicalcium phosphate/polylactide-co-glycolide) was analyzed. Animals were divided in two separate groups. The first group (A) was the control one. In the second group (B) the implantation site was submitted to HeNe laser irradiation. Animals were sacrificed two, six, eight and twelve weeks after laser therapy. Histological samples were decalcified and submerged in paraffin blocks. Samples were colored with HE. Morphometrical analysis consisted of measuring area fraction, area, integral optical density, mean density and density variation. Results showed beneficial effect of laser treatment in reparation of alveolar bone after two and six weeks. After eight and twelve weeks no beneficial laser effect on biocomposite osseointegration could be noticed.