Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold
Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA
Authors
Vasiljević, Perica
Najman, Stevo

Đorđević, Ljubiša
Savić, Vojin P.
Vukelić, Marija Đ.
Živanov Čurlis, Jelena Z.
Ignjatović, Nenad

Uskoković, Dragan

Article (Published version)
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Show full item recordAbstract
Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and... 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone.
Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.
Source:
Hemijska industrija, 2009, 63, 4, 301-307Publisher:
- Belgrade : Association of the Chemical Engineers of Serbia
Funding / projects:
- Regeneracija skeletnih tkiva pomognuta biomaterijalima kao tkivnim matricama - in vivo i in vitro studija (RS-145072)
DOI: 10.2298/HEMIND0904301V
ISSN: 0367-598X
WoS: 000271316600004
Scopus: 2-s2.0-77952945230
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Vasiljević, Perica AU - Najman, Stevo AU - Đorđević, Ljubiša AU - Savić, Vojin P. AU - Vukelić, Marija Đ. AU - Živanov Čurlis, Jelena Z. AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2009 UR - http://www.ncbi.nlm.nih.gov/pubmed/0367-598X0904301V UR - https://dais.sanu.ac.rs/123456789/3362 AB - Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone. AB - Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu. PB - Belgrade : Association of the Chemical Engineers of Serbia T2 - Hemijska industrija T1 - Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold T1 - Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA SP - 301 EP - 307 VL - 63 IS - 4 DO - 10.2298/HEMIND0904301V UR - https://hdl.handle.net/21.15107/rcub_dais_3362 ER -
@article{ author = "Vasiljević, Perica and Najman, Stevo and Đorđević, Ljubiša and Savić, Vojin P. and Vukelić, Marija Đ. and Živanov Čurlis, Jelena Z. and Ignjatović, Nenad and Uskoković, Dragan", year = "2009", abstract = "Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone., Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.", publisher = "Belgrade : Association of the Chemical Engineers of Serbia", journal = "Hemijska industrija", title = "Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold, Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA", pages = "301-307", volume = "63", number = "4", doi = "10.2298/HEMIND0904301V", url = "https://hdl.handle.net/21.15107/rcub_dais_3362" }
Vasiljević, P., Najman, S., Đorđević, L., Savić, V. P., Vukelić, M. Đ., Živanov Čurlis, J. Z., Ignjatović, N.,& Uskoković, D.. (2009). Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold. in Hemijska industrija Belgrade : Association of the Chemical Engineers of Serbia., 63(4), 301-307. https://doi.org/10.2298/HEMIND0904301V https://hdl.handle.net/21.15107/rcub_dais_3362
Vasiljević P, Najman S, Đorđević L, Savić VP, Vukelić MĐ, Živanov Čurlis JZ, Ignjatović N, Uskoković D. Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold. in Hemijska industrija. 2009;63(4):301-307. doi:10.2298/HEMIND0904301V https://hdl.handle.net/21.15107/rcub_dais_3362 .
Vasiljević, Perica, Najman, Stevo, Đorđević, Ljubiša, Savić, Vojin P., Vukelić, Marija Đ., Živanov Čurlis, Jelena Z., Ignjatović, Nenad, Uskoković, Dragan, "Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold" in Hemijska industrija, 63, no. 4 (2009):301-307, https://doi.org/10.2298/HEMIND0904301V ., https://hdl.handle.net/21.15107/rcub_dais_3362 .