The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells
Authorized Users Only
2017
Authors
Najdanović, JelenaNajman, Stevo

Stojanović, Sanja

Živković, Jelena M.
Ignjatović, Nenad

Uskoković, Dragan

Trajanović, Miroslav

Contributors
Lee, BillGadow, Rainer

Mitić, Vojislav
Conference object (Published version)

Metadata
Show full item recordAbstract
Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLG...A. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.
Keywords:
calcium phosphate/poly-(dl-lactide-co-glycolide) / nanomaterials / HeLa cells / biomedical materialsSource:
Proceedings of the IV Advanced Ceramics and Applications Conference, 2017, 387-400Publisher:
- Paris : Atlantis Press
Funding / projects:
- Virtual human osteoarticular system and its application in preclinical and clinical practice (RS-41017)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Najdanović, Jelena AU - Najman, Stevo AU - Stojanović, Sanja AU - Živković, Jelena M. AU - Ignjatović, Nenad AU - Uskoković, Dragan AU - Trajanović, Miroslav PY - 2017 UR - https://dais.sanu.ac.rs/123456789/2379 AB - Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment. PB - Paris : Atlantis Press C3 - Proceedings of the IV Advanced Ceramics and Applications Conference T1 - The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells SP - 387 EP - 400 DO - 10.2991/978-94-6239-213-7_27 UR - https://hdl.handle.net/21.15107/rcub_dais_2379 ER -
@conference{ editor = "Lee, Bill, Gadow, Rainer, Mitić, Vojislav", author = "Najdanović, Jelena and Najman, Stevo and Stojanović, Sanja and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Trajanović, Miroslav", year = "2017", abstract = "Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.", publisher = "Paris : Atlantis Press", journal = "Proceedings of the IV Advanced Ceramics and Applications Conference", title = "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells", pages = "387-400", doi = "10.2991/978-94-6239-213-7_27", url = "https://hdl.handle.net/21.15107/rcub_dais_2379" }
Lee, B., Gadow, R., Mitić, V., Najdanović, J., Najman, S., Stojanović, S., Živković, J. M., Ignjatović, N., Uskoković, D.,& Trajanović, M.. (2017). The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference Paris : Atlantis Press., 387-400. https://doi.org/10.2991/978-94-6239-213-7_27 https://hdl.handle.net/21.15107/rcub_dais_2379
Lee B, Gadow R, Mitić V, Najdanović J, Najman S, Stojanović S, Živković JM, Ignjatović N, Uskoković D, Trajanović M. The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference. 2017;:387-400. doi:10.2991/978-94-6239-213-7_27 https://hdl.handle.net/21.15107/rcub_dais_2379 .
Lee, Bill, Gadow, Rainer, Mitić, Vojislav, Najdanović, Jelena, Najman, Stevo, Stojanović, Sanja, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Trajanović, Miroslav, "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells" in Proceedings of the IV Advanced Ceramics and Applications Conference (2017):387-400, https://doi.org/10.2991/978-94-6239-213-7_27 ., https://hdl.handle.net/21.15107/rcub_dais_2379 .