A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide
Abstract
The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fi...ckian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.
Keywords:
poly-dl-lactide-co-glycolide / tigecycline / nanoparticles / calcium phosphate / drug delivery / PLGA / HApSource:
Journal of Materials Science: Materials in Medicine, 2010, 21, 1, 231-239Publisher:
- Springer US
Funding / projects:
- Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou (RS-142006)
DOI: 10.1007/s10856-009-3854-6
ISSN: 0957-4530; 1573-4838 (online)
WoS: 000273592200026
Scopus: 2-s2.0-75049085742
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ignjatović, Nenad AU - Ninkov, Petar AU - Sabetrasekh, Roya AU - Uskoković, Dragan PY - 2010 UR - https://dais.sanu.ac.rs/123456789/179 AB - The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects. PB - Springer US T2 - Journal of Materials Science: Materials in Medicine T1 - A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide SP - 231 EP - 239 VL - 21 IS - 1 DO - 10.1007/s10856-009-3854-6 UR - https://hdl.handle.net/21.15107/rcub_dais_179 ER -
@article{ author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Uskoković, Dragan", year = "2010", abstract = "The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.", publisher = "Springer US", journal = "Journal of Materials Science: Materials in Medicine", title = "A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide", pages = "231-239", volume = "21", number = "1", doi = "10.1007/s10856-009-3854-6", url = "https://hdl.handle.net/21.15107/rcub_dais_179" }
Ignjatović, N., Ninkov, P., Sabetrasekh, R.,& Uskoković, D.. (2010). A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. in Journal of Materials Science: Materials in Medicine Springer US., 21(1), 231-239. https://doi.org/10.1007/s10856-009-3854-6 https://hdl.handle.net/21.15107/rcub_dais_179
Ignjatović N, Ninkov P, Sabetrasekh R, Uskoković D. A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. in Journal of Materials Science: Materials in Medicine. 2010;21(1):231-239. doi:10.1007/s10856-009-3854-6 https://hdl.handle.net/21.15107/rcub_dais_179 .
Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Uskoković, Dragan, "A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide" in Journal of Materials Science: Materials in Medicine, 21, no. 1 (2010):231-239, https://doi.org/10.1007/s10856-009-3854-6 ., https://hdl.handle.net/21.15107/rcub_dais_179 .