Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: Influence of the process parameters
Abstract
Biodegradable microspheres such as those made of poly-D,L-lactide (PDLLA) are widely investigated delivery systems for drugs or antigens. The aim of this study was to examine experimental conditions in order to produce PDLLA microspheres with the best properties for controlled and sustained drug delivery by the modified precipitation method. For this purpose, the following parameters were varied: co-solvent (methanol or ethanol), the concentration of stabilizer polyvinyl alcohol (PVA), chloroform-to-water ratio and the speed and time of homogenization. Scanning electron microscopy (SEM) and stereological analysis were used to characterize the particles. The average size and morphology of the microspheres varied substantially with preparation conditions from 8.44–1.25 μm. Results showed that the smallest particles were obtained with methanol, 1% PVA and with 10 min of homogenization at 21,000 rpm. Microsc. Res. Tech., 2008. © 2007 Wiley-Liss, Inc.
Keywords:
microspheres / poly(D,L-lactide) / modified precipitation method / scanning electron microscopy / stereological analysis / PDLLASource:
Microscopy Research and Technique, 2008, 72, 2, 86-92Publisher:
- Hoboken, NJ : John Wiley & Sons
Funding / projects:
- Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou (RS-142006)
DOI: 10.1002/jemt.20529
ISSN: 1097-0029 (online); 1059-910X
WoS: 000253361200002
Scopus: 2-s2.0-39449102972
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Jovanović, Ivana AU - Jordović, Branka AU - Petković, Marijana AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2008 UR - https://dais.sanu.ac.rs/123456789/177 AB - Biodegradable microspheres such as those made of poly-D,L-lactide (PDLLA) are widely investigated delivery systems for drugs or antigens. The aim of this study was to examine experimental conditions in order to produce PDLLA microspheres with the best properties for controlled and sustained drug delivery by the modified precipitation method. For this purpose, the following parameters were varied: co-solvent (methanol or ethanol), the concentration of stabilizer polyvinyl alcohol (PVA), chloroform-to-water ratio and the speed and time of homogenization. Scanning electron microscopy (SEM) and stereological analysis were used to characterize the particles. The average size and morphology of the microspheres varied substantially with preparation conditions from 8.44–1.25 μm. Results showed that the smallest particles were obtained with methanol, 1% PVA and with 10 min of homogenization at 21,000 rpm. Microsc. Res. Tech., 2008. © 2007 Wiley-Liss, Inc. PB - Hoboken, NJ : John Wiley & Sons T2 - Microscopy Research and Technique T1 - Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: Influence of the process parameters SP - 86 EP - 92 VL - 72 IS - 2 DO - 10.1002/jemt.20529 UR - https://hdl.handle.net/21.15107/rcub_dais_177 ER -
@article{ author = "Jovanović, Ivana and Jordović, Branka and Petković, Marijana and Ignjatović, Nenad and Uskoković, Dragan", year = "2008", abstract = "Biodegradable microspheres such as those made of poly-D,L-lactide (PDLLA) are widely investigated delivery systems for drugs or antigens. The aim of this study was to examine experimental conditions in order to produce PDLLA microspheres with the best properties for controlled and sustained drug delivery by the modified precipitation method. For this purpose, the following parameters were varied: co-solvent (methanol or ethanol), the concentration of stabilizer polyvinyl alcohol (PVA), chloroform-to-water ratio and the speed and time of homogenization. Scanning electron microscopy (SEM) and stereological analysis were used to characterize the particles. The average size and morphology of the microspheres varied substantially with preparation conditions from 8.44–1.25 μm. Results showed that the smallest particles were obtained with methanol, 1% PVA and with 10 min of homogenization at 21,000 rpm. Microsc. Res. Tech., 2008. © 2007 Wiley-Liss, Inc.", publisher = "Hoboken, NJ : John Wiley & Sons", journal = "Microscopy Research and Technique", title = "Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: Influence of the process parameters", pages = "86-92", volume = "72", number = "2", doi = "10.1002/jemt.20529", url = "https://hdl.handle.net/21.15107/rcub_dais_177" }
Jovanović, I., Jordović, B., Petković, M., Ignjatović, N.,& Uskoković, D.. (2008). Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: Influence of the process parameters. in Microscopy Research and Technique Hoboken, NJ : John Wiley & Sons., 72(2), 86-92. https://doi.org/10.1002/jemt.20529 https://hdl.handle.net/21.15107/rcub_dais_177
Jovanović I, Jordović B, Petković M, Ignjatović N, Uskoković D. Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: Influence of the process parameters. in Microscopy Research and Technique. 2008;72(2):86-92. doi:10.1002/jemt.20529 https://hdl.handle.net/21.15107/rcub_dais_177 .
Jovanović, Ivana, Jordović, Branka, Petković, Marijana, Ignjatović, Nenad, Uskoković, Dragan, "Preparation of smallest microparticles of poly-D,L-lactide by modified precipitation method: Influence of the process parameters" in Microscopy Research and Technique, 72, no. 2 (2008):86-92, https://doi.org/10.1002/jemt.20529 ., https://hdl.handle.net/21.15107/rcub_dais_177 .