Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles
Аутори
Piljević, MiljanaDinić, Ivana
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Bukumira, Marta
Rabasović, Mihailo
![](/themes/MirageDAIS/images/orcid.png)
Krmpot, Aleksandar
![](/themes/MirageDAIS/images/orcid.png)
Lazarević, Miloš
Mančić, Lidija
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Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease.
Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared
(NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific a...ntibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and
photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells.
Кључне речи:
nanoparticles / up-conversion / lanthanide / photoluminescenceИзвор:
Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024, 2024, 38-38Издавач:
- Belgrade : Institute of Physics
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200175 (Институт техничких наука САНУ, Београд) (RS-MESTD-inst-2020-200175)
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Piljević, Miljana AU - Dinić, Ivana AU - Bukumira, Marta AU - Rabasović, Mihailo AU - Krmpot, Aleksandar AU - Lazarević, Miloš AU - Mančić, Lidija PY - 2024 UR - https://dais.sanu.ac.rs/123456789/16596 AB - Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease. Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared (NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific antibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells. PB - Belgrade : Institute of Physics C3 - Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 T1 - Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles SP - 38 EP - 38 UR - https://hdl.handle.net/21.15107/rcub_dais_16596 ER -
@conference{ author = "Piljević, Miljana and Dinić, Ivana and Bukumira, Marta and Rabasović, Mihailo and Krmpot, Aleksandar and Lazarević, Miloš and Mančić, Lidija", year = "2024", abstract = "Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease. Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared (NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific antibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells.", publisher = "Belgrade : Institute of Physics", journal = "Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024", title = "Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles", pages = "38-38", url = "https://hdl.handle.net/21.15107/rcub_dais_16596" }
Piljević, M., Dinić, I., Bukumira, M., Rabasović, M., Krmpot, A., Lazarević, M.,& Mančić, L.. (2024). Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles. in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 Belgrade : Institute of Physics., 38-38. https://hdl.handle.net/21.15107/rcub_dais_16596
Piljević M, Dinić I, Bukumira M, Rabasović M, Krmpot A, Lazarević M, Mančić L. Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles. in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024. 2024;:38-38. https://hdl.handle.net/21.15107/rcub_dais_16596 .
Piljević, Miljana, Dinić, Ivana, Bukumira, Marta, Rabasović, Mihailo, Krmpot, Aleksandar, Lazarević, Miloš, Mančić, Lidija, "Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles" in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 (2024):38-38, https://hdl.handle.net/21.15107/rcub_dais_16596 .