Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline
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2016
Authors
Radovanović, MirjanaJugović, Branimir

Gvozdenović, Milica M.

Jokić, Bojan

Grgur, Branimir

Bugarski, Branko

Knežević Jugović, Zorica

Article (Published version)

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The immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be... 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability.
Keywords:
α-amylase / immobilization / adsorption / magnetic particles / polyanilineSource:
Starch, 2016, 68, 5-6, 427-435Publisher:
- Hoboken, NJ : John Wiley & Sons
Funding / projects:
DOI: 10.1002/star.201500161
ISSN: 1521-379X
WoS: 000380156500006
Scopus: 2-s2.0-84949255495
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Radovanović, Mirjana AU - Jugović, Branimir AU - Gvozdenović, Milica M. AU - Jokić, Bojan AU - Grgur, Branimir AU - Bugarski, Branko AU - Knežević Jugović, Zorica PY - 2016 UR - https://dais.sanu.ac.rs/123456789/16002 AB - The immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability. PB - Hoboken, NJ : John Wiley & Sons T2 - Starch T1 - Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline SP - 427 EP - 435 VL - 68 IS - 5-6 DO - 10.1002/star.201500161 UR - https://hdl.handle.net/21.15107/rcub_dais_16002 ER -
@article{ author = "Radovanović, Mirjana and Jugović, Branimir and Gvozdenović, Milica M. and Jokić, Bojan and Grgur, Branimir and Bugarski, Branko and Knežević Jugović, Zorica", year = "2016", abstract = "The immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability.", publisher = "Hoboken, NJ : John Wiley & Sons", journal = "Starch", title = "Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline", pages = "427-435", volume = "68", number = "5-6", doi = "10.1002/star.201500161", url = "https://hdl.handle.net/21.15107/rcub_dais_16002" }
Radovanović, M., Jugović, B., Gvozdenović, M. M., Jokić, B., Grgur, B., Bugarski, B.,& Knežević Jugović, Z.. (2016). Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline. in Starch Hoboken, NJ : John Wiley & Sons., 68(5-6), 427-435. https://doi.org/10.1002/star.201500161 https://hdl.handle.net/21.15107/rcub_dais_16002
Radovanović M, Jugović B, Gvozdenović MM, Jokić B, Grgur B, Bugarski B, Knežević Jugović Z. Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline. in Starch. 2016;68(5-6):427-435. doi:10.1002/star.201500161 https://hdl.handle.net/21.15107/rcub_dais_16002 .
Radovanović, Mirjana, Jugović, Branimir, Gvozdenović, Milica M., Jokić, Bojan, Grgur, Branimir, Bugarski, Branko, Knežević Jugović, Zorica, "Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline" in Starch, 68, no. 5-6 (2016):427-435, https://doi.org/10.1002/star.201500161 ., https://hdl.handle.net/21.15107/rcub_dais_16002 .