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Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities

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2013
1948.pdf (2.248Mb)
Authors
Stevanović, Magdalena
Uskoković, Vuk
Filipović, Miloš
Škapin, Srečo Davor
Uskoković, Dragan
Article (Accepted Version)
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Abstract
The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended... antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.

Keywords:
nanotechnology / PLGA / silver nanoparticles / osteoinductivity / antibacterial materials
Source:
ACS Applied Materials and Interfaces, 2013, 5, 18, 9034-9042
Funding / projects:
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
  • United States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
Note:
  • This is the peer-reviewed version of the articleStevanović, M., Uskoković, V., Filipović, M., Škapin, S.D., Uskoković, D., 2013. Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities. ACS Applied Materials & Interfaces 5, 9034–9042. https://doi.org/10.1021/am402237g

DOI: 10.1021/am402237g

ISSN: 1944-8244 (Print); 1944-8252 (Online)

WoS: 000330016500031

Scopus: 2-s2.0-84885049659
[ Google Scholar ]
30
33
Handle
https://hdl.handle.net/21.15107/rcub_dais_15986
URI
https://dais.sanu.ac.rs/123456789/15986
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Uskoković, Vuk
AU  - Filipović, Miloš
AU  - Škapin, Srečo Davor
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/15986
AB  - The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.
T2  - ACS Applied Materials and Interfaces
T1  - Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities
SP  - 9034
EP  - 9042
VL  - 5
IS  - 18
DO  - 10.1021/am402237g
UR  - https://hdl.handle.net/21.15107/rcub_dais_15986
ER  - 
@article{
author = "Stevanović, Magdalena and Uskoković, Vuk and Filipović, Miloš and Škapin, Srečo Davor and Uskoković, Dragan",
year = "2013",
abstract = "The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.",
journal = "ACS Applied Materials and Interfaces",
title = "Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities",
pages = "9034-9042",
volume = "5",
number = "18",
doi = "10.1021/am402237g",
url = "https://hdl.handle.net/21.15107/rcub_dais_15986"
}
Stevanović, M., Uskoković, V., Filipović, M., Škapin, S. D.,& Uskoković, D.. (2013). Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities. in ACS Applied Materials and Interfaces, 5(18), 9034-9042.
https://doi.org/10.1021/am402237g
https://hdl.handle.net/21.15107/rcub_dais_15986
Stevanović M, Uskoković V, Filipović M, Škapin SD, Uskoković D. Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities. in ACS Applied Materials and Interfaces. 2013;5(18):9034-9042.
doi:10.1021/am402237g
https://hdl.handle.net/21.15107/rcub_dais_15986 .
Stevanović, Magdalena, Uskoković, Vuk, Filipović, Miloš, Škapin, Srečo Davor, Uskoković, Dragan, "Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities" in ACS Applied Materials and Interfaces, 5, no. 18 (2013):9034-9042,
https://doi.org/10.1021/am402237g .,
https://hdl.handle.net/21.15107/rcub_dais_15986 .

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