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Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies

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2016
1944.pdf (2.210Mb)
Authors
Stojanović, Zoran S.
Ignjatović, Nenad
Wu, Victoria
Žunič, Vojka
Veselinović, Ljiljana
Škapin, Srečo Davor
Miljković, Miroslav
Uskoković, Vuk
Uskoković, Dragan
Article (Accepted Version)
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Abstract
Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibit...ed excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.

Keywords:
hydrothermal processing / nanowires / hydroxyapatite / biomedical materials / particle size distribution
Source:
Materials Science and Engineering: C, 2016, 68, 746-757
Funding / projects:
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
  • United States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
Note:
  • This is the peer-reviewed version of the articleStojanović, Z.S., Ignjatović, N., Wu, V., Žunič, V., Veselinović, L., Škapin, S., Miljković, M., Uskoković, V., Uskoković, D., 2016. Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. Materials Science and Engineering: C 68, 746–757. https://doi.org/10.1016/j.msec.2016.06.047

DOI: 10.1016/j.msec.2016.06.047

ISSN: 0928-4931

WoS: 000382600000088

Scopus: 2-s2.0-84975886009
[ Google Scholar ]
31
24
Handle
https://hdl.handle.net/21.15107/rcub_dais_15985
URI
https://dais.sanu.ac.rs/123456789/15985
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Stojanović, Zoran S.
AU  - Ignjatović, Nenad
AU  - Wu, Victoria
AU  - Žunič, Vojka
AU  - Veselinović, Ljiljana
AU  - Škapin, Srečo Davor
AU  - Miljković, Miroslav
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/15985
AB  - Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.
T2  - Materials Science and Engineering: C
T1  - Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies
SP  - 746
EP  - 757
VL  - 68
DO  - 10.1016/j.msec.2016.06.047
UR  - https://hdl.handle.net/21.15107/rcub_dais_15985
ER  - 
@article{
author = "Stojanović, Zoran S. and Ignjatović, Nenad and Wu, Victoria and Žunič, Vojka and Veselinović, Ljiljana and Škapin, Srečo Davor and Miljković, Miroslav and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.",
journal = "Materials Science and Engineering: C",
title = "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies",
pages = "746-757",
volume = "68",
doi = "10.1016/j.msec.2016.06.047",
url = "https://hdl.handle.net/21.15107/rcub_dais_15985"
}
Stojanović, Z. S., Ignjatović, N., Wu, V., Žunič, V., Veselinović, L., Škapin, S. D., Miljković, M., Uskoković, V.,& Uskoković, D.. (2016). Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C, 68, 746-757.
https://doi.org/10.1016/j.msec.2016.06.047
https://hdl.handle.net/21.15107/rcub_dais_15985
Stojanović ZS, Ignjatović N, Wu V, Žunič V, Veselinović L, Škapin SD, Miljković M, Uskoković V, Uskoković D. Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C. 2016;68:746-757.
doi:10.1016/j.msec.2016.06.047
https://hdl.handle.net/21.15107/rcub_dais_15985 .
Stojanović, Zoran S., Ignjatović, Nenad, Wu, Victoria, Žunič, Vojka, Veselinović, Ljiljana, Škapin, Srečo Davor, Miljković, Miroslav, Uskoković, Vuk, Uskoković, Dragan, "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies" in Materials Science and Engineering: C, 68 (2016):746-757,
https://doi.org/10.1016/j.msec.2016.06.047 .,
https://hdl.handle.net/21.15107/rcub_dais_15985 .

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