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dc.creatorIgnjatović, Nenad
dc.creatorWu, Victoria
dc.creatorAjduković, Zorica
dc.creatorMihajilov Krstev, Tatjana
dc.creatorUskoković, Vuk
dc.creatorUskoković, Dragan
dc.date.accessioned2018-01-30T10:09:10Z
dc.date.available2017-11-24
dc.date.issued2016
dc.identifier.issn0928-4931 (Print)
dc.identifier.issn1873-0191 (Online)
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/15983
dc.description.abstractComposite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.eng
dc.format60 (2016) 357-364
dc.formatapplication/pdf
dc.languageen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.relationUnited States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
dc.rightsembargoedAccess
dc.sourceMaterials science & engineering. C, Materials for biological applicationseng
dc.subjectantimicrobial activity
dc.subjectbone regeneration
dc.subjectchitosan
dc.subjecthydroxyapatite
dc.subjectPLGA
dc.titleChitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissueseng
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractУскоковић, Вук; Ускоковић, Драган; Михајилов Крстев, Татјана; Aјдуковић, Зорица; Wу, Вицториа; Игњатовић, Ненад;
dc.citation.spage357
dc.citation.epage364
dc.citation.volume60
dc.identifier.wos000369204700042
dc.identifier.doi10.1016/j.msec.2015.11.061
dc.identifier.scopus2-s2.0-84948422411
dc.description.otherThis is the peer-reviewed version of the articleIgnjatović, N., Wu, V., Ajduković, Z., Mihajilov-Krstev, T., Uskoković, V., Uskoković, D., 2016. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. Materials Science and Engineering: C 60, 357–364. [https://doi.org/10.1016/j.msec.2015.11.061]
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