Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose

2016
Authors
Jugović, Branimir
Grgur, Branimir

Antov, Mirjana

Knežević Jugović, Zorica

Stevanović, Jasmina S.

Gvozdenović, Milica M.

Article (Published version)
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Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Keywords:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensorsSource:
International Journal of Electrochemcal Science, 2016, 11, 2, 1152-1161Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Jugović, Branimir AU - Grgur, Branimir AU - Antov, Mirjana AU - Knežević Jugović, Zorica AU - Stevanović, Jasmina S. AU - Gvozdenović, Milica M. PY - 2016 UR - https://dais.sanu.ac.rs/123456789/15979 AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. T2 - International Journal of Electrochemcal Science T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose SP - 1152 EP - 1161 VL - 11 IS - 2 UR - https://hdl.handle.net/21.15107/rcub_dais_15979 ER -
@article{ author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević Jugović, Zorica and Stevanović, Jasmina S. and Gvozdenović, Milica M.", year = "2016", abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.", journal = "International Journal of Electrochemcal Science", title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose", pages = "1152-1161", volume = "11", number = "2", url = "https://hdl.handle.net/21.15107/rcub_dais_15979" }
Jugović, B., Grgur, B., Antov, M., Knežević Jugović, Z., Stevanović, J. S.,& Gvozdenović, M. M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemcal Science, 11(2), 1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979
Jugović B, Grgur B, Antov M, Knežević Jugović Z, Stevanović JS, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemcal Science. 2016;11(2):1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979 .
Jugović, Branimir, Grgur, Branimir, Antov, Mirjana, Knežević Jugović, Zorica, Stevanović, Jasmina S., Gvozdenović, Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemcal Science, 11, no. 2 (2016):1152-1161, https://hdl.handle.net/21.15107/rcub_dais_15979 .