Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
TY - JOUR
AU - Jugović, Branimir
AU - Grgur, Branimir
AU - Antov, Mirjana
AU - Knežević Jugović, Zorica
AU - Stevanović, Jasmina S.
AU - Gvozdenović, Milica M.
PY - 2016
UR - http://dais.sanu.ac.rs/123456789/15979
AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
T2 - International Journal of Electrochemcal Science
T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
SP - 1152
EP - 1161
VL - 11
IS - 2
ER -
@article{
author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević Jugović, Zorica and Stevanović, Jasmina S. and Gvozdenović, Milica M.",
year = "2016",
url = "http://dais.sanu.ac.rs/123456789/15979",
abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.",
journal = "International Journal of Electrochemcal Science",
title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose",
pages = "1152-1161",
volume = "11",
number = "2"
}
Jugović B, Grgur B, Antov M, Knežević Jugović Z, Stevanović JS, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. International Journal of Electrochemcal Science. 2016;11(2):1152-1161
Jugović, B., Grgur, B., Antov, M., Knežević Jugović, Z., Stevanović, J. S.,& Gvozdenović, M. M. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose.
International Journal of Electrochemcal Science, 11(2), 1152-1161.
Jugović Branimir, Grgur Branimir, Antov Mirjana, Knežević Jugović Zorica, Stevanović Jasmina S., Gvozdenović Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" 11, no. 2 (2016):1152-1161
