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Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose

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2016
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Authors
Jugović, Branimir
Grgur, Branimir
Antov, Mirjana
Knežević Jugović, Zorica
Stevanović, Jasmina S.
Gvozdenović, Milica M.
Article (Published version)
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Abstract
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Keywords:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensors
Source:
International Journal of Electrochemcal Science, 2016, 11, 2, 1152-1161
Projects:
  • Electrochemical synthesis and characterization of nanostructured functional materials for application in new technologies (RS-172046)

ISSN: 1452-3981

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URI
http://dais.sanu.ac.rs/123456789/15979
Collections
  • ITN SANU - Opšta kolekcija / ITS SASA - General collection
Institution
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Jugović, Branimir
AU  - Grgur, Branimir
AU  - Antov, Mirjana
AU  - Knežević Jugović, Zorica
AU  - Stevanović, Jasmina S.
AU  - Gvozdenović, Milica M.
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/15979
AB  - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
T2  - International Journal of Electrochemcal Science
T1  - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
SP  - 1152
EP  - 1161
VL  - 11
IS  - 2
ER  - 
@article{
author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević Jugović, Zorica and Stevanović, Jasmina S. and Gvozdenović, Milica M.",
year = "2016",
url = "http://dais.sanu.ac.rs/123456789/15979",
abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.",
journal = "International Journal of Electrochemcal Science",
title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose",
pages = "1152-1161",
volume = "11",
number = "2"
}
Jugović B, Grgur B, Antov M, Knežević Jugović Z, Stevanović JS, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. International Journal of Electrochemcal Science. 2016;11(2):1152-1161
Jugović, B., Grgur, B., Antov, M., Knežević Jugović, Z., Stevanović, J. S.,& Gvozdenović, M. M. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose.
International Journal of Electrochemcal Science, 11(2), 1152-1161. 
Jugović Branimir, Grgur Branimir, Antov Mirjana, Knežević Jugović Zorica, Stevanović Jasmina S., Gvozdenović Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" 11, no. 2 (2016):1152-1161

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