Photo-driven upconversion in NaYF4:Yb,Er@chitosane particles for cancer cells bioimaging
Abstract
Lanthanide doped up-converting nanoparticles (UCNPs) have significant role in biomedical field, especially in cell imaging and target drug delivery, due to their convenient luminescent properties. For that purpose UCNPs should have the specific morphological and luminescent characteristics. In this study the biocompatible NaYF4:Yb,Er@Chitosane particles were synthesized through one-step hydrothermal synthesis. Obtained particles were characterized in detail using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Structural refinement data show the presence of cubic (Fm-3m) phase in spherical shaped nanoparticles size up to 200 nm, while the FTIR analysis revealed the presence of chitosan on the particle surface which have no influence on the luminescence efficiency of the UCNPs. Cytotoxicity as well as cell labeling capability of synthesized U...CNPs were tested in vitro on the human gingival fibroblasts (HGF) and head and neck squamous carcinoma cells (HNSCC). The results show excellent biocompatibility against HGF, and successful in- vitro visualization of HNSCC cell cultures upon 980 nm laser irradiation.
Keywords:
NaYF4:Yb,Er@chitosan / nanoparticles / luminescenceSource:
Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and Engineering, December 6-8, 2017, Belgrade, Serbia, 2017, 2-2Publisher:
- Belgrade : Institute of Technical Sciences of SASA
Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Dinić, Ivana AU - Đukić Vuković, Aleksandra AU - Nikolić, Marko G. AU - Milošević, Olivera AU - Mančić, Lidija PY - 2017 UR - https://dais.sanu.ac.rs/123456789/15448 AB - Lanthanide doped up-converting nanoparticles (UCNPs) have significant role in biomedical field, especially in cell imaging and target drug delivery, due to their convenient luminescent properties. For that purpose UCNPs should have the specific morphological and luminescent characteristics. In this study the biocompatible NaYF4:Yb,Er@Chitosane particles were synthesized through one-step hydrothermal synthesis. Obtained particles were characterized in detail using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Structural refinement data show the presence of cubic (Fm-3m) phase in spherical shaped nanoparticles size up to 200 nm, while the FTIR analysis revealed the presence of chitosan on the particle surface which have no influence on the luminescence efficiency of the UCNPs. Cytotoxicity as well as cell labeling capability of synthesized UCNPs were tested in vitro on the human gingival fibroblasts (HGF) and head and neck squamous carcinoma cells (HNSCC). The results show excellent biocompatibility against HGF, and successful in- vitro visualization of HNSCC cell cultures upon 980 nm laser irradiation. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and Engineering, December 6-8, 2017, Belgrade, Serbia T1 - Photo-driven upconversion in NaYF4:Yb,Er@chitosane particles for cancer cells bioimaging SP - 2 EP - 2 UR - https://hdl.handle.net/21.15107/rcub_dais_15448 ER -
@conference{ author = "Dinić, Ivana and Đukić Vuković, Aleksandra and Nikolić, Marko G. and Milošević, Olivera and Mančić, Lidija", year = "2017", abstract = "Lanthanide doped up-converting nanoparticles (UCNPs) have significant role in biomedical field, especially in cell imaging and target drug delivery, due to their convenient luminescent properties. For that purpose UCNPs should have the specific morphological and luminescent characteristics. In this study the biocompatible NaYF4:Yb,Er@Chitosane particles were synthesized through one-step hydrothermal synthesis. Obtained particles were characterized in detail using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Structural refinement data show the presence of cubic (Fm-3m) phase in spherical shaped nanoparticles size up to 200 nm, while the FTIR analysis revealed the presence of chitosan on the particle surface which have no influence on the luminescence efficiency of the UCNPs. Cytotoxicity as well as cell labeling capability of synthesized UCNPs were tested in vitro on the human gingival fibroblasts (HGF) and head and neck squamous carcinoma cells (HNSCC). The results show excellent biocompatibility against HGF, and successful in- vitro visualization of HNSCC cell cultures upon 980 nm laser irradiation.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and Engineering, December 6-8, 2017, Belgrade, Serbia", title = "Photo-driven upconversion in NaYF4:Yb,Er@chitosane particles for cancer cells bioimaging", pages = "2-2", url = "https://hdl.handle.net/21.15107/rcub_dais_15448" }
Dinić, I., Đukić Vuković, A., Nikolić, M. G., Milošević, O.,& Mančić, L.. (2017). Photo-driven upconversion in NaYF4:Yb,Er@chitosane particles for cancer cells bioimaging. in Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and Engineering, December 6-8, 2017, Belgrade, Serbia Belgrade : Institute of Technical Sciences of SASA., 2-2. https://hdl.handle.net/21.15107/rcub_dais_15448
Dinić I, Đukić Vuković A, Nikolić MG, Milošević O, Mančić L. Photo-driven upconversion in NaYF4:Yb,Er@chitosane particles for cancer cells bioimaging. in Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and Engineering, December 6-8, 2017, Belgrade, Serbia. 2017;:2-2. https://hdl.handle.net/21.15107/rcub_dais_15448 .
Dinić, Ivana, Đukić Vuković, Aleksandra, Nikolić, Marko G., Milošević, Olivera, Mančić, Lidija, "Photo-driven upconversion in NaYF4:Yb,Er@chitosane particles for cancer cells bioimaging" in Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and Engineering, December 6-8, 2017, Belgrade, Serbia (2017):2-2, https://hdl.handle.net/21.15107/rcub_dais_15448 .