Приказ основних података о документу
Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
dc.creator | Kuzminac, Ivana | |
dc.creator | Ćelić, Andjelka S. | |
dc.creator | Bekić, Sofija S. | |
dc.creator | Kojić, Vesna | |
dc.creator | Savić, Marina P. | |
dc.creator | Ignjatović, Nenad | |
dc.date.accessioned | 2022-06-22T12:24:45Z | |
dc.date.available | 2022-06-22T12:24:45Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | https://dais.sanu.ac.rs/123456789/13030 | |
dc.description.abstract | Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy. | |
dc.publisher | Elsevier BV | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200175/RS// | |
dc.relation | Provincial Secretariat for Higher Education and Scientific Research of the Autonomous Province of Vojvodina, Project: New steroid derivatives - potential chemotherapeutics, No. 142–451-2667/2021 | |
dc.relation | COST Action CA 17140 “Cancer Nanomedicine from the Bench to the Bedside” | |
dc.relation.isversionof | https://hdl.handle.net/21.15107/rcub_dais_13030 | |
dc.rights | restrictedAccess | |
dc.source | Colloids and Surfaces B: Biointerfaces | en |
dc.subject | androstane | |
dc.subject | D-homo lactone | |
dc.subject | nanocarriers | |
dc.subject | estrogen | |
dc.subject | androgen receptors | |
dc.subject | cancer | |
dc.title | Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells | en |
dc.type | article | en |
dc.rights.license | ARR | en |
dc.citation.spage | 112597 | |
dc.citation.volume | 216 | |
dc.identifier.wos | 00080799480000 | |
dc.identifier.doi | 10.1016/j.colsurfb.2022.112597 | |
dc.identifier.scopus | 2-s2.0-85131462166 | |
dc.description.other | Peer-reviewed manuscript: [https://hdl.handle.net/21.15107/rcub_dais_13030] | |
dc.type.version | publishedVersion | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_dais_13030 |