Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells
Authors
Brdarić, EmilijaSoković Bajić, Svetlana
Đokić, Jelena
Đurđić, Slađana
Ruas-Madiedo, Patricia
Stevanović, Magdalena

Tolinački, Maja
Dinić, Miroslav

Mutić, Jelena
Golić, Nataša
Živković, Milica
Article (Published version)
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Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.
Source:
Frontiers in Microbiology, 2021, 12, 3222-Publisher:
- Frontiers
Funding / projects:
DOI: 10.3389/fmicb.2021.759378
ISSN: 1664-302X
WoS: 000719997300001
Scopus: 2-s2.0-85119152907
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Brdarić, Emilija AU - Soković Bajić, Svetlana AU - Đokić, Jelena AU - Đurđić, Slađana AU - Ruas-Madiedo, Patricia AU - Stevanović, Magdalena AU - Tolinački, Maja AU - Dinić, Miroslav AU - Mutić, Jelena AU - Golić, Nataša AU - Živković, Milica PY - 2021 UR - https://dais.sanu.ac.rs/123456789/12357 AB - Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage. PB - Frontiers T2 - Frontiers in Microbiology T1 - Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells SP - 3222 VL - 12 DO - 10.3389/fmicb.2021.759378 UR - https://hdl.handle.net/21.15107/rcub_dais_12357 ER -
@article{ author = "Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Đurđić, Slađana and Ruas-Madiedo, Patricia and Stevanović, Magdalena and Tolinački, Maja and Dinić, Miroslav and Mutić, Jelena and Golić, Nataša and Živković, Milica", year = "2021", abstract = "Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.", publisher = "Frontiers", journal = "Frontiers in Microbiology", title = "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells", pages = "3222", volume = "12", doi = "10.3389/fmicb.2021.759378", url = "https://hdl.handle.net/21.15107/rcub_dais_12357" }
Brdarić, E., Soković Bajić, S., Đokić, J., Đurđić, S., Ruas-Madiedo, P., Stevanović, M., Tolinački, M., Dinić, M., Mutić, J., Golić, N.,& Živković, M.. (2021). Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology Frontiers., 12, 3222. https://doi.org/10.3389/fmicb.2021.759378 https://hdl.handle.net/21.15107/rcub_dais_12357
Brdarić E, Soković Bajić S, Đokić J, Đurđić S, Ruas-Madiedo P, Stevanović M, Tolinački M, Dinić M, Mutić J, Golić N, Živković M. Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology. 2021;12:3222. doi:10.3389/fmicb.2021.759378 https://hdl.handle.net/21.15107/rcub_dais_12357 .
Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Đurđić, Slađana, Ruas-Madiedo, Patricia, Stevanović, Magdalena, Tolinački, Maja, Dinić, Miroslav, Mutić, Jelena, Golić, Nataša, Živković, Milica, "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells" in Frontiers in Microbiology, 12 (2021):3222, https://doi.org/10.3389/fmicb.2021.759378 ., https://hdl.handle.net/21.15107/rcub_dais_12357 .