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Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples

Authorized Users Only
2023
Authors
Stanković, Mira
Bartolić, Dragana
Mutavdžić, Dragosav
Marković, Smilja
Grubić, Saša
Jovanović, Nemanja M.
Radotić, Ksenija
Article (Published version)
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Abstract
In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R2 was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS... could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.

Keywords:
Apis mellifera / differential scanning calorimetry / fluorescence / honey / infection / MCR-ALS method
Source:
Journal of Apicultural Research, 2023, 62, 3, 507-513
Publisher:
  • Taylor and Francis
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-200175)

DOI: 10.1080/00218839.2021.1889803

ISSN: 0021-8839

WoS: 000625791800001

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_dais_12336
URI
https://dais.sanu.ac.rs/123456789/12336
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Mutavdžić, Dragosav
AU  - Marković, Smilja
AU  - Grubić, Saša
AU  - Jovanović, Nemanja M.
AU  - Radotić, Ksenija
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/12336
AB  - In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R2 was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples
SP  - 507
EP  - 513
VL  - 62
IS  - 3
DO  - 10.1080/00218839.2021.1889803
UR  - https://hdl.handle.net/21.15107/rcub_dais_12336
ER  - 
@article{
author = "Stanković, Mira and Bartolić, Dragana and Mutavdžić, Dragosav and Marković, Smilja and Grubić, Saša and Jovanović, Nemanja M. and Radotić, Ksenija",
year = "2023",
abstract = "In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R2 was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.",
publisher = "Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples",
pages = "507-513",
volume = "62",
number = "3",
doi = "10.1080/00218839.2021.1889803",
url = "https://hdl.handle.net/21.15107/rcub_dais_12336"
}
Stanković, M., Bartolić, D., Mutavdžić, D., Marković, S., Grubić, S., Jovanović, N. M.,& Radotić, K.. (2023). Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research
Taylor and Francis., 62(3), 507-513.
https://doi.org/10.1080/00218839.2021.1889803
https://hdl.handle.net/21.15107/rcub_dais_12336
Stanković M, Bartolić D, Mutavdžić D, Marković S, Grubić S, Jovanović NM, Radotić K. Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research. 2023;62(3):507-513.
doi:10.1080/00218839.2021.1889803
https://hdl.handle.net/21.15107/rcub_dais_12336 .
Stanković, Mira, Bartolić, Dragana, Mutavdžić, Dragosav, Marković, Smilja, Grubić, Saša, Jovanović, Nemanja M., Radotić, Ksenija, "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples" in Journal of Apicultural Research, 62, no. 3 (2023):507-513,
https://doi.org/10.1080/00218839.2021.1889803 .,
https://hdl.handle.net/21.15107/rcub_dais_12336 .

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