Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles
Authors
Rabasović, Mihailo D.
Dinić, Ivana

Đukić Vuković, Aleksandra

Lazarević, Miloš
Nikolić, Marko G.

Krmpot, Aleksandar

Mančić, Lidija

Conference object (Published version)
Metadata
Show full item recordAbstract
The Nonlinear Laser Scanning Microscopy (NLSM) contributes to the cell labeling through addressing two main issues: photobleaching and phototoxicity. Moreover, an increase of the penetration depth and a reduction of background autofluorescence are achieved.We have used a multidisciplinary approach combining expertise in material science, nanoparticles synthesis and characterization, cancer cell and tissue labeling, and high resolution imaging, in order to accomplish in vitro imaging of the cancer cells. We have imaged the oral squamous carcinoma cells and human gingival cells. We have demonstrated that we are able to take high contrast images. We have shown position of the nanoparticles in cells, through colocalization of the cell auto-fluorescence and the nanoparticles up-conversion.We plan to improve our abilities through further optimization of the up-converting nanoparticles (smaller and brighter particles) and microscopy technique.
Keywords:
Nonlinear Laser Scanning Microscopy / NLSM / cell labelling / photobleaching / phototoxicity / up-converting nanoparticlesSource:
Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36, 2021, 35-35Publisher:
- Belgrade : Serbian Ceramic Society
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200024 (University of Belgrade, Institute of Physics, Belgrade-Zemun) (RS-200024)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-200175)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-200135)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200129 (University of Belgrade, Faculty of Dental Medicine) (RS-200129)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Rabasović, Mihailo D. AU - Dinić, Ivana AU - Đukić Vuković, Aleksandra AU - Lazarević, Miloš AU - Nikolić, Marko G. AU - Krmpot, Aleksandar AU - Mančić, Lidija PY - 2021 UR - https://dais.sanu.ac.rs/123456789/11912 AB - The Nonlinear Laser Scanning Microscopy (NLSM) contributes to the cell labeling through addressing two main issues: photobleaching and phototoxicity. Moreover, an increase of the penetration depth and a reduction of background autofluorescence are achieved.We have used a multidisciplinary approach combining expertise in material science, nanoparticles synthesis and characterization, cancer cell and tissue labeling, and high resolution imaging, in order to accomplish in vitro imaging of the cancer cells. We have imaged the oral squamous carcinoma cells and human gingival cells. We have demonstrated that we are able to take high contrast images. We have shown position of the nanoparticles in cells, through colocalization of the cell auto-fluorescence and the nanoparticles up-conversion.We plan to improve our abilities through further optimization of the up-converting nanoparticles (smaller and brighter particles) and microscopy technique. PB - Belgrade : Serbian Ceramic Society C3 - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36 T1 - Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles SP - 35 EP - 35 UR - https://hdl.handle.net/21.15107/rcub_dais_11912 ER -
@conference{ author = "Rabasović, Mihailo D. and Dinić, Ivana and Đukić Vuković, Aleksandra and Lazarević, Miloš and Nikolić, Marko G. and Krmpot, Aleksandar and Mančić, Lidija", year = "2021", abstract = "The Nonlinear Laser Scanning Microscopy (NLSM) contributes to the cell labeling through addressing two main issues: photobleaching and phototoxicity. Moreover, an increase of the penetration depth and a reduction of background autofluorescence are achieved.We have used a multidisciplinary approach combining expertise in material science, nanoparticles synthesis and characterization, cancer cell and tissue labeling, and high resolution imaging, in order to accomplish in vitro imaging of the cancer cells. We have imaged the oral squamous carcinoma cells and human gingival cells. We have demonstrated that we are able to take high contrast images. We have shown position of the nanoparticles in cells, through colocalization of the cell auto-fluorescence and the nanoparticles up-conversion.We plan to improve our abilities through further optimization of the up-converting nanoparticles (smaller and brighter particles) and microscopy technique.", publisher = "Belgrade : Serbian Ceramic Society", journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36", title = "Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles", pages = "35-35", url = "https://hdl.handle.net/21.15107/rcub_dais_11912" }
Rabasović, M. D., Dinić, I., Đukić Vuković, A., Lazarević, M., Nikolić, M. G., Krmpot, A.,& Mančić, L.. (2021). Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36 Belgrade : Serbian Ceramic Society., 35-35. https://hdl.handle.net/21.15107/rcub_dais_11912
Rabasović MD, Dinić I, Đukić Vuković A, Lazarević M, Nikolić MG, Krmpot A, Mančić L. Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36. 2021;:35-35. https://hdl.handle.net/21.15107/rcub_dais_11912 .
Rabasović, Mihailo D., Dinić, Ivana, Đukić Vuković, Aleksandra, Lazarević, Miloš, Nikolić, Marko G., Krmpot, Aleksandar, Mančić, Lidija, "Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36 (2021):35-35, https://hdl.handle.net/21.15107/rcub_dais_11912 .