On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation
Article (Published version)
Metadata
Show full item recordAbstract
Nucleation of minerals in the presence of additives is critical for achieving control over the formation of solids in biomineralization processes or during syntheses of advanced hybrid materials. Herein, we investigated the early stages of Fe(III) (oxy)(hydr)oxide formation with/without polyglutamic acid (pGlu) at low driving force for phase separation (pH 2.0 to 3.0). We employed an advanced pH-constant titration assay, X-ray diffraction, thermal analysis with mass spectrometry, Fourier Transform infrared spectroscopy, and scanning electron microscopy. Three stages were observed: initial binding, stabilization of Fe(III) pre-nucleation clusters (PNCs), and phase separation, yielding Fe(III) (oxy)(hydr)oxide. The data suggest that organic–inorganic interactions occurred via binding of olation Fe(III) PNC species. Fourier Transform Infrared Spectroscopy (FTIR) analyses revealed a plausible interaction motif and a conformational adaptation of the polypeptide. The stabilization of the aqu...eous Fe(III) system against nucleation by pGlu contrasts with the previously reported influence of poly-aspartic acid (pAsp). While this is difficult to explain based on classical nucleation theory, alternative notions such as the so-called PNC pathway provide a possible rationale. Developing a nucleation theory that successfully explains and predicts distinct influences for chemically similar additives like pAsp and pGlu is the Holy Grail toward advancing the knowledge of nucleation, early growth, and structure formation.
Keywords:
pre-nucleation clusters / iron (III) hydrolysis / poly-L-glutamic acid / nucleation / additive-controlled mineralizationSource:
Minerals, 2021, 11, 7, 715-Publisher:
- Basel : MDPI AG
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-200175)
- Deutscher Akademischer Austauschdienst http://dx.doi.org/10.13039/501100001655 : IASTE stipend
- IAESTE Belgrade
DOI: 10.3390/min11070715
ISSN: 2075-163X
WoS: 000676411100001
Scopus: 2-s2.0-85108945942
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Lukić, Miodrag J. AU - Lücke, Felix AU - Ilić, Teodora AU - Petrović, Katharina AU - Gebauer, Denis PY - 2021 UR - https://dais.sanu.ac.rs/123456789/11802 AB - Nucleation of minerals in the presence of additives is critical for achieving control over the formation of solids in biomineralization processes or during syntheses of advanced hybrid materials. Herein, we investigated the early stages of Fe(III) (oxy)(hydr)oxide formation with/without polyglutamic acid (pGlu) at low driving force for phase separation (pH 2.0 to 3.0). We employed an advanced pH-constant titration assay, X-ray diffraction, thermal analysis with mass spectrometry, Fourier Transform infrared spectroscopy, and scanning electron microscopy. Three stages were observed: initial binding, stabilization of Fe(III) pre-nucleation clusters (PNCs), and phase separation, yielding Fe(III) (oxy)(hydr)oxide. The data suggest that organic–inorganic interactions occurred via binding of olation Fe(III) PNC species. Fourier Transform Infrared Spectroscopy (FTIR) analyses revealed a plausible interaction motif and a conformational adaptation of the polypeptide. The stabilization of the aqueous Fe(III) system against nucleation by pGlu contrasts with the previously reported influence of poly-aspartic acid (pAsp). While this is difficult to explain based on classical nucleation theory, alternative notions such as the so-called PNC pathway provide a possible rationale. Developing a nucleation theory that successfully explains and predicts distinct influences for chemically similar additives like pAsp and pGlu is the Holy Grail toward advancing the knowledge of nucleation, early growth, and structure formation. PB - Basel : MDPI AG T2 - Minerals T1 - On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation SP - 715 VL - 11 IS - 7 DO - 10.3390/min11070715 UR - https://hdl.handle.net/21.15107/rcub_dais_11802 ER -
@article{ author = "Lukić, Miodrag J. and Lücke, Felix and Ilić, Teodora and Petrović, Katharina and Gebauer, Denis", year = "2021", abstract = "Nucleation of minerals in the presence of additives is critical for achieving control over the formation of solids in biomineralization processes or during syntheses of advanced hybrid materials. Herein, we investigated the early stages of Fe(III) (oxy)(hydr)oxide formation with/without polyglutamic acid (pGlu) at low driving force for phase separation (pH 2.0 to 3.0). We employed an advanced pH-constant titration assay, X-ray diffraction, thermal analysis with mass spectrometry, Fourier Transform infrared spectroscopy, and scanning electron microscopy. Three stages were observed: initial binding, stabilization of Fe(III) pre-nucleation clusters (PNCs), and phase separation, yielding Fe(III) (oxy)(hydr)oxide. The data suggest that organic–inorganic interactions occurred via binding of olation Fe(III) PNC species. Fourier Transform Infrared Spectroscopy (FTIR) analyses revealed a plausible interaction motif and a conformational adaptation of the polypeptide. The stabilization of the aqueous Fe(III) system against nucleation by pGlu contrasts with the previously reported influence of poly-aspartic acid (pAsp). While this is difficult to explain based on classical nucleation theory, alternative notions such as the so-called PNC pathway provide a possible rationale. Developing a nucleation theory that successfully explains and predicts distinct influences for chemically similar additives like pAsp and pGlu is the Holy Grail toward advancing the knowledge of nucleation, early growth, and structure formation.", publisher = "Basel : MDPI AG", journal = "Minerals", title = "On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation", pages = "715", volume = "11", number = "7", doi = "10.3390/min11070715", url = "https://hdl.handle.net/21.15107/rcub_dais_11802" }
Lukić, M. J., Lücke, F., Ilić, T., Petrović, K.,& Gebauer, D.. (2021). On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation. in Minerals Basel : MDPI AG., 11(7), 715. https://doi.org/10.3390/min11070715 https://hdl.handle.net/21.15107/rcub_dais_11802
Lukić MJ, Lücke F, Ilić T, Petrović K, Gebauer D. On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation. in Minerals. 2021;11(7):715. doi:10.3390/min11070715 https://hdl.handle.net/21.15107/rcub_dais_11802 .
Lukić, Miodrag J., Lücke, Felix, Ilić, Teodora, Petrović, Katharina, Gebauer, Denis, "On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation" in Minerals, 11, no. 7 (2021):715, https://doi.org/10.3390/min11070715 ., https://hdl.handle.net/21.15107/rcub_dais_11802 .