Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition
Authors
Pantović-Pavlović, Marijana
Pavlović, Miroslav

Kovačina, Jovanka
Stanojević, Boris
Stevanović, Jasmina

Panić, Vladimir

Ignjatović, Nenad

Article (Published version)
Metadata
Show full item recordAbstract
The cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line was elucidated. ACP/TiO2 and ACP/TiO2/ /ChOL were deposited onto Ti by a novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells exposed to composite samples without chitosan lactate, while there was an increase in the number of viable cells in the sample containing ChOL. These findings show that there was improved cell proliferation, differentiation and cell viability in the ChOL-containing sample, which makes ACP/TiO2/ChOL coating a good candidate for applications in medicine and stomatology.
Keywords:
cytotoxicity / dye exclusion test / colorimetric tests / tetrazolium salts / amorphous materials / calcium phosphate / chitosan oligolactateSource:
Journal of the Serbian Chemical Society, 2021, 86, 6, 555-559Publisher:
- Belgrade : Serbian Chemical Society
Funding / projects:
- http://dx.doi.org/10.13039/501100004564
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-200175)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
DOI: 10.2298/JSC210211024P
ISSN: 0352-5139; 1820-7421
WoS: 000669486500002
Scopus: 2-s2.0-85110726365
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Pantović-Pavlović, Marijana AU - Pavlović, Miroslav AU - Kovačina, Jovanka AU - Stanojević, Boris AU - Stevanović, Jasmina AU - Panić, Vladimir AU - Ignjatović, Nenad PY - 2021 UR - https://dais.sanu.ac.rs/123456789/11738 AB - The cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line was elucidated. ACP/TiO2 and ACP/TiO2/ /ChOL were deposited onto Ti by a novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells exposed to composite samples without chitosan lactate, while there was an increase in the number of viable cells in the sample containing ChOL. These findings show that there was improved cell proliferation, differentiation and cell viability in the ChOL-containing sample, which makes ACP/TiO2/ChOL coating a good candidate for applications in medicine and stomatology. PB - Belgrade : Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition SP - 555 EP - 559 VL - 86 IS - 6 DO - 10.2298/JSC210211024P UR - https://hdl.handle.net/21.15107/rcub_dais_11738 ER -
@article{ author = "Pantović-Pavlović, Marijana and Pavlović, Miroslav and Kovačina, Jovanka and Stanojević, Boris and Stevanović, Jasmina and Panić, Vladimir and Ignjatović, Nenad", year = "2021", abstract = "The cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line was elucidated. ACP/TiO2 and ACP/TiO2/ /ChOL were deposited onto Ti by a novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells exposed to composite samples without chitosan lactate, while there was an increase in the number of viable cells in the sample containing ChOL. These findings show that there was improved cell proliferation, differentiation and cell viability in the ChOL-containing sample, which makes ACP/TiO2/ChOL coating a good candidate for applications in medicine and stomatology.", publisher = "Belgrade : Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition", pages = "555-559", volume = "86", number = "6", doi = "10.2298/JSC210211024P", url = "https://hdl.handle.net/21.15107/rcub_dais_11738" }
Pantović-Pavlović, M., Pavlović, M., Kovačina, J., Stanojević, B., Stevanović, J., Panić, V.,& Ignjatović, N.. (2021). Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition. in Journal of the Serbian Chemical Society Belgrade : Serbian Chemical Society., 86(6), 555-559. https://doi.org/10.2298/JSC210211024P https://hdl.handle.net/21.15107/rcub_dais_11738
Pantović-Pavlović M, Pavlović M, Kovačina J, Stanojević B, Stevanović J, Panić V, Ignjatović N. Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition. in Journal of the Serbian Chemical Society. 2021;86(6):555-559. doi:10.2298/JSC210211024P https://hdl.handle.net/21.15107/rcub_dais_11738 .
Pantović-Pavlović, Marijana, Pavlović, Miroslav, Kovačina, Jovanka, Stanojević, Boris, Stevanović, Jasmina, Panić, Vladimir, Ignjatović, Nenad, "Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition" in Journal of the Serbian Chemical Society, 86, no. 6 (2021):555-559, https://doi.org/10.2298/JSC210211024P ., https://hdl.handle.net/21.15107/rcub_dais_11738 .