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dc.creatorUšjak, Dušan
dc.creatorDinić, Miroslav
dc.creatorNovović, Katarina
dc.creatorIvković, Branka
dc.creatorFilipović, Nenad
dc.creatorStevanović, Magdalena
dc.creatorMilenković, Marina T.
dc.date.accessioned2020-12-22T04:51:40Z
dc.date.available2020-12-22T04:51:40Z
dc.date.issued2021
dc.identifier.issn1612-1872
dc.identifier.issn1612-1880
dc.identifier.urihttps://dais.sanu.ac.rs/123456789/10034
dc.description.abstractAn increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.en
dc.publisherWileyen
dc.relation.isversionofhttps://doi.org/10.1002/cbdv.202000786
dc.relation.isversionofhttps://hdl.handle.net/21.15107/rcub_dais_11878
dc.relation.isreferencedbyhttps://hdl.handle.net/21.15107/rcub_dais_10086
dc.relation.isreferencedbyhttps://hdl.handle.net/21.15107/rcub_dais_11879
dc.relation.replaceshttps://hdl.handle.net/21.15107/rcub_dais_10086
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceChemistry & Biodiversityen
dc.subjectAcinetobacter baumannii
dc.subjectnosocomial pathogens
dc.subjectbiofilms
dc.subjectgene expression
dc.subjectpolymerase chain reaction
dc.subjectvirulence factors
dc.titleMethoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expressionen
dc.typearticleen
dc.rights.licenseBY
dcterms.abstractСтевановић, Магдалена; Ушјак, Душан; Филиповић, Ненад; Миленковић, Марина Т.; Динић, Мирослав; Нововић, Катарина; Ивковић, Бранка;
dc.citation.spagee2000786
dc.citation.volume18
dc.citation.issue1
dc.identifier.wos000596697700001
dc.identifier.doi10.1002/cbdv.202000786
dc.identifier.scopus2-s2.0-85097239451
dc.description.otherThis is the peer reviewed version of the article: Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M., Milenković, M.T., n.d. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. Chemistry & Biodiversity n/a. [https://doi.org/10.1002/cbdv.202000786]
dc.description.otherPoster: [https://hdl.handle.net/21.15107/rcub_dais_10086]
dc.description.otherSupporting information: [https://hdl.handle.net/21.15107/rcub_dais_11879]
dc.type.versionacceptedVersion
dc.identifier.fulltexthttps://dais.sanu.ac.rs/bitstream/id/41328/10.1002@cbdv.202000786.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_dais_10034


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