Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression
Аутори
Ušjak, DušanDinić, Miroslav
Novović, Katarina
Ivković, Branka
Filipović, Nenad
Stevanović, Magdalena
Milenković, Marina T.
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusi...on is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
Кључне речи:
Acinetobacter baumannii / nosocomial pathogens / biofilms / gene expression / polymerase chain reaction / virulence factorsИзвор:
Chemistry & Biodiversity, 2021, 18, 1, e2000786-Издавач:
- Wiley
Напомена:
- This is the peer reviewed version of the article: Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M., Milenković, M.T., n.d. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. Chemistry & Biodiversity n/a. https://doi.org/10.1002/cbdv.202000786
- Poster: https://hdl.handle.net/21.15107/rcub_dais_10086
- Supporting information: https://hdl.handle.net/21.15107/rcub_dais_11879
Повезане информације:
- Друга верзија
https://doi.org/10.1002/cbdv.202000786 - Друга верзија
https://hdl.handle.net/21.15107/rcub_dais_11878 - Повезани садржај
https://hdl.handle.net/21.15107/rcub_dais_10086 - Повезани садржај
https://hdl.handle.net/21.15107/rcub_dais_11879
DOI: 10.1002/cbdv.202000786
ISSN: 1612-1872; 1612-1880
WoS: 000596697700001
Scopus: 2-s2.0-85097239451
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ušjak, Dušan AU - Dinić, Miroslav AU - Novović, Katarina AU - Ivković, Branka AU - Filipović, Nenad AU - Stevanović, Magdalena AU - Milenković, Marina T. PY - 2021 UR - https://dais.sanu.ac.rs/123456789/10034 AB - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility. PB - Wiley T2 - Chemistry & Biodiversity T1 - Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression SP - e2000786 VL - 18 IS - 1 DO - 10.1002/cbdv.202000786 UR - https://hdl.handle.net/21.15107/rcub_dais_10034 ER -
@article{ author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.", year = "2021", abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.", publisher = "Wiley", journal = "Chemistry & Biodiversity", title = "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression", pages = "e2000786", volume = "18", number = "1", doi = "10.1002/cbdv.202000786", url = "https://hdl.handle.net/21.15107/rcub_dais_10034" }
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2021). Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity Wiley., 18(1), e2000786. https://doi.org/10.1002/cbdv.202000786 https://hdl.handle.net/21.15107/rcub_dais_10034
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity. 2021;18(1):e2000786. doi:10.1002/cbdv.202000786 https://hdl.handle.net/21.15107/rcub_dais_10034 .
Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786 ., https://hdl.handle.net/21.15107/rcub_dais_10034 .