Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression
Authors
Ušjak, Dušan
Dinić, Miroslav

Novović, Katarina

Ivković, Branka

Filipović, Nenad

Stevanović, Magdalena

Milenković, Marina T.

Article (Accepted Version)
Metadata
Show full item recordAbstract
An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusi...on is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
Keywords:
Acinetobacter baumannii / nosocomial pathogens / biofilms / gene expression / polymerase chain reaction / virulence factorsSource:
Chemistry & Biodiversity, 2021, 18, 1, e2000786-Publisher:
- Wiley
Note:
- This is the peer reviewed version of the article: Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M., Milenković, M.T., n.d. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. Chemistry & Biodiversity n/a. https://doi.org/10.1002/cbdv.202000786
- Poster: https://hdl.handle.net/21.15107/rcub_dais_10086
- Supporting information: https://hdl.handle.net/21.15107/rcub_dais_11879
Related info:
- Version of
https://doi.org/10.1002/cbdv.202000786 - Version of
https://hdl.handle.net/21.15107/rcub_dais_11878 - Referenced by
https://hdl.handle.net/21.15107/rcub_dais_10086 - Referenced by
https://hdl.handle.net/21.15107/rcub_dais_11879
DOI: 10.1002/cbdv.202000786
ISSN: 1612-1872; 1612-1880
WoS: 000596697700001
Scopus: 2-s2.0-85097239451
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ušjak, Dušan AU - Dinić, Miroslav AU - Novović, Katarina AU - Ivković, Branka AU - Filipović, Nenad AU - Stevanović, Magdalena AU - Milenković, Marina T. PY - 2021 UR - https://dais.sanu.ac.rs/123456789/10034 AB - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility. PB - Wiley T2 - Chemistry & Biodiversity T1 - Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression SP - e2000786 VL - 18 IS - 1 DO - 10.1002/cbdv.202000786 UR - https://hdl.handle.net/21.15107/rcub_dais_10034 ER -
@article{ author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.", year = "2021", abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.", publisher = "Wiley", journal = "Chemistry & Biodiversity", title = "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression", pages = "e2000786", volume = "18", number = "1", doi = "10.1002/cbdv.202000786", url = "https://hdl.handle.net/21.15107/rcub_dais_10034" }
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2021). Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity Wiley., 18(1), e2000786. https://doi.org/10.1002/cbdv.202000786 https://hdl.handle.net/21.15107/rcub_dais_10034
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity. 2021;18(1):e2000786. doi:10.1002/cbdv.202000786 https://hdl.handle.net/21.15107/rcub_dais_10034 .
Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786 ., https://hdl.handle.net/21.15107/rcub_dais_10034 .