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Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression

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2020
10.1002@cbdv.202000786.pdf (2.360Mb)
Authors
Ušjak, Dušan
Dinić, Miroslav
Novović, Katarina
Ivković, Branka
Filipović, Nenad
Stevanović, Magdalena
Milenković, Marina T.
Article (Accepted Version)
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Abstract
An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusi...on is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.

Keywords:
Acinetobacter baumannii / nosocomial pathogens / biofilms / gene expression / polymerase chain reaction / virulence factors
Source:
Chemistry & Biodiversity, 2020
Publisher:
  • Wiley
Note:
  • This is the peer reviewed version of the article: Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M., Milenković, M.T., n.d. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. Chemistry & Biodiversity n/a. https://doi.org/10.1002/cbdv.202000786
  • Poster: https://dais.sanu.ac.rs/handle/123456789/10086

DOI: 10.1002/cbdv.202000786

ISSN: 1612-1872; 1612-1880

WoS: 000596697700001

Scopus: 2-s2.0-85097239451
[ Google Scholar ]
URI
https://dais.sanu.ac.rs/123456789/10034
Collections
  • ITN SANU - Opšta kolekcija / ITS SASA - General collection
Institution
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina T.
PY  - 2020
UR  - https://dais.sanu.ac.rs/123456789/10034
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley
T2  - Chemistry & Biodiversity
T1  - Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression
DO  - 10.1002/cbdv.202000786
ER  - 
@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.",
year = "2020",
url = "https://dais.sanu.ac.rs/123456789/10034",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity",
title = "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression",
doi = "10.1002/cbdv.202000786"
}
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression. Chemistry & Biodiversity. 2020;
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T. (2020). Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression.
Chemistry & BiodiversityWiley., null. 
https://doi.org/10.1002/cbdv.202000786
Ušjak Dušan, Dinić Miroslav, Novović Katarina, Ivković Branka, Filipović Nenad, Stevanović Magdalena, Milenković Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression" null (2020),
https://doi.org/10.1002/cbdv.202000786 .

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