Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200129 (University of Belgrade, Faculty of Dental Medicine)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200129 (University of Belgrade, Faculty of Dental Medicine) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200129 (Univerzitet u Beogradu, Stomatološki fakultet) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200129 (Универзитет у Београду, Стоматолошки факултет) (sr)
Authors

Publications

Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles

Rabasović, Mihailo D.; Dinić, Ivana; Đukić Vuković, Aleksandra; Lazarević, Miloš; Nikolić, Marko G.; Krmpot, Aleksandar; Mančić, Lidija

(Belgrade : Serbian Ceramic Society, 2021)

TY  - CONF
AU  - Rabasović, Mihailo D.
AU  - Dinić, Ivana
AU  - Đukić Vuković, Aleksandra
AU  - Lazarević, Miloš
AU  - Nikolić, Marko G.
AU  - Krmpot, Aleksandar
AU  - Mančić, Lidija
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11912
AB  - The Nonlinear Laser Scanning Microscopy (NLSM) contributes to the cell labeling through addressing two main issues: photobleaching and phototoxicity. Moreover, an increase of the penetration depth and a reduction of background autofluorescence are achieved.We have used a multidisciplinary approach combining expertise in material science, nanoparticles synthesis and characterization, cancer cell and tissue labeling, and high resolution imaging, in order to accomplish in vitro imaging of the cancer cells. We have imaged the oral squamous carcinoma cells and human gingival cells. We have demonstrated that we are able to take high contrast images. We have shown position of the nanoparticles in cells, through colocalization of the cell auto-fluorescence and the nanoparticles up-conversion.We plan to improve our abilities through further optimization of the up-converting nanoparticles (smaller and brighter particles) and microscopy technique.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36
T1  - Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles
SP  - 35
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_dais_11912
ER  - 
@conference{
author = "Rabasović, Mihailo D. and Dinić, Ivana and Đukić Vuković, Aleksandra and Lazarević, Miloš and Nikolić, Marko G. and Krmpot, Aleksandar and Mančić, Lidija",
year = "2021",
abstract = "The Nonlinear Laser Scanning Microscopy (NLSM) contributes to the cell labeling through addressing two main issues: photobleaching and phototoxicity. Moreover, an increase of the penetration depth and a reduction of background autofluorescence are achieved.We have used a multidisciplinary approach combining expertise in material science, nanoparticles synthesis and characterization, cancer cell and tissue labeling, and high resolution imaging, in order to accomplish in vitro imaging of the cancer cells. We have imaged the oral squamous carcinoma cells and human gingival cells. We have demonstrated that we are able to take high contrast images. We have shown position of the nanoparticles in cells, through colocalization of the cell auto-fluorescence and the nanoparticles up-conversion.We plan to improve our abilities through further optimization of the up-converting nanoparticles (smaller and brighter particles) and microscopy technique.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36",
title = "Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles",
pages = "35-35",
url = "https://hdl.handle.net/21.15107/rcub_dais_11912"
}
Rabasović, M. D., Dinić, I., Đukić Vuković, A., Lazarević, M., Nikolić, M. G., Krmpot, A.,& Mančić, L.. (2021). Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36
Belgrade : Serbian Ceramic Society., 35-35.
https://hdl.handle.net/21.15107/rcub_dais_11912
Rabasović MD, Dinić I, Đukić Vuković A, Lazarević M, Nikolić MG, Krmpot A, Mančić L. Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36. 2021;:35-35.
https://hdl.handle.net/21.15107/rcub_dais_11912 .
Rabasović, Mihailo D., Dinić, Ivana, Đukić Vuković, Aleksandra, Lazarević, Miloš, Nikolić, Marko G., Krmpot, Aleksandar, Mančić, Lidija, "Nonlinear laser scanning microscopy for imaging of the cells labeled by upconverting NaYF4:Yb,Er nanoparticles" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application IX : New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 20-21. September 2021, 2021, 36-36 (2021):35-35,
https://hdl.handle.net/21.15107/rcub_dais_11912 .