Virtual human osteoarticular system and its application in preclinical and clinical practice

Link to this page

info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41017/RS//

Virtual human osteoarticular system and its application in preclinical and clinical practice (en)
Виртуелни коштано зглобни систем човека и његова примена у претклиничкој и клиничкој пракси (sr)
Virtuelni koštano zglobni sistem čoveka i njegova primena u pretkliničkoj i kliničkoj praksi (sr_RS)
Authors

Publications

Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro

Tomić, Milica; Stojanović, Sanja; Ignjatović, Nenad; Najman, Stevo

(Belgrade : Institute of Technical Sciences of SASA, 2019)

TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/6969
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
T1  - Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro
SP  - 4
EP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_dais_6969
ER  - 
@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2019",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia",
title = "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro",
pages = "4-4",
url = "https://hdl.handle.net/21.15107/rcub_dais_6969"
}
Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2019). Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 4-4.
https://hdl.handle.net/21.15107/rcub_dais_6969
Tomić M, Stojanović S, Ignjatović N, Najman S. Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:4-4.
https://hdl.handle.net/21.15107/rcub_dais_6969 .
Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro" in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia (2019):4-4,
https://hdl.handle.net/21.15107/rcub_dais_6969 .

In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay

Stojanović, Sanja; Bajić, Lazar; Cvetković, Vladimir; Ignjatović, Nenad; Najman, Stevo

(Belgrade : Institute of Technical Sciences of SASA, 2018)

TY  - CONF
AU  - Stojanović, Sanja
AU  - Bajić, Lazar
AU  - Cvetković, Vladimir
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2018
UR  - http://dais.sanu.ac.rs/123456789/4723
AB  - Nanomaterials represent a relatively new and developing class of materials that are widely used because of their unique mechanical and physiochemical properties. Due to the particles’ size and the ability to easily enter the cells and interact with different cellular components, nanoparticles can exhibit toxic effects in biological systems, so it is necessary to conduct a thorough in vitro biocompatibility testing. Our aim was to examine the in vitro genotoxic and apoptotic potential of two different synthesized nanomaterials intended for bone tissue engineering and regenerative applications: biphasic calcium phosphate coated with poly- D,L-lactide-co-glycolide (CP/PLGA) and cobalt-substituted hydroxyapatite (CoHAp), by DNA fragmentation assay. NB4 cells (acute promyelocytic leukemia cell line) were incubated with different concentrations of CP/PLGA and CoHAp nanomaterials for 24 hours. Cells cultured in standard medium, without nanomaterials, were used as negative control and cells treated with hydrogen peroxide were used as positive control. After incubation with materials the genomic DNA was extracted from the cells and applied on horizontal agarose gel electrophoresis. The results of DNA fragmentation assay were observed on gel documentation system. Higher examined concentrations of CP/PLGA caused DNA fragmentation at some extent with DNA laddering at approximately 180 bp which is characteristic for later stages of apoptosis, while DNA laddering was not seen in the case of CoHAp at the same concentrations. Although both materials are in the form of nanoparticles, a different effect in apoptotic DNA fragmentation was observed which suggests that the chemical composition of nanomaterials notably affects the genotoxicity and apoptosis potential in addition to the particle size.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
T1  - In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_dais_4723
ER  - 
@conference{
author = "Stojanović, Sanja and Bajić, Lazar and Cvetković, Vladimir and Ignjatović, Nenad and Najman, Stevo",
year = "2018",
abstract = "Nanomaterials represent a relatively new and developing class of materials that are widely used because of their unique mechanical and physiochemical properties. Due to the particles’ size and the ability to easily enter the cells and interact with different cellular components, nanoparticles can exhibit toxic effects in biological systems, so it is necessary to conduct a thorough in vitro biocompatibility testing. Our aim was to examine the in vitro genotoxic and apoptotic potential of two different synthesized nanomaterials intended for bone tissue engineering and regenerative applications: biphasic calcium phosphate coated with poly- D,L-lactide-co-glycolide (CP/PLGA) and cobalt-substituted hydroxyapatite (CoHAp), by DNA fragmentation assay. NB4 cells (acute promyelocytic leukemia cell line) were incubated with different concentrations of CP/PLGA and CoHAp nanomaterials for 24 hours. Cells cultured in standard medium, without nanomaterials, were used as negative control and cells treated with hydrogen peroxide were used as positive control. After incubation with materials the genomic DNA was extracted from the cells and applied on horizontal agarose gel electrophoresis. The results of DNA fragmentation assay were observed on gel documentation system. Higher examined concentrations of CP/PLGA caused DNA fragmentation at some extent with DNA laddering at approximately 180 bp which is characteristic for later stages of apoptosis, while DNA laddering was not seen in the case of CoHAp at the same concentrations. Although both materials are in the form of nanoparticles, a different effect in apoptotic DNA fragmentation was observed which suggests that the chemical composition of nanomaterials notably affects the genotoxicity and apoptosis potential in addition to the particle size.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia",
title = "In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_dais_4723"
}
Stojanović, S., Bajić, L., Cvetković, V., Ignjatović, N.,& Najman, S.. (2018). In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 69-69.
https://hdl.handle.net/21.15107/rcub_dais_4723
Stojanović S, Bajić L, Cvetković V, Ignjatović N, Najman S. In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia. 2018;:69-69.
https://hdl.handle.net/21.15107/rcub_dais_4723 .
Stojanović, Sanja, Bajić, Lazar, Cvetković, Vladimir, Ignjatović, Nenad, Najman, Stevo, "In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay" in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia (2018):69-69,
https://hdl.handle.net/21.15107/rcub_dais_4723 .

The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells

Lee, Bill; Gadow, Rainer; Mitić, Vojislav; Najdanović, Jelena; Najman, Stevo; Stojanović, Sanja; Živković, Jelena M.; Ignjatović, Nenad; Uskoković, Dragan; Trajanović, Miroslav

(Paris : Atlantis Press, 2017)

TY  - CONF
AU  - Najdanović, Jelena
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Trajanović, Miroslav
PY  - 2017
UR  - http://dais.sanu.ac.rs/123456789/2379
AB  - Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.
PB  - Paris : Atlantis Press
C3  - Proceedings of the IV Advanced Ceramics and Applications Conference
T1  - The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells
SP  - 387
EP  - 400
DO  - 10.2991/978-94-6239-213-7_27
UR  - https://hdl.handle.net/21.15107/rcub_dais_2379
ER  - 
@conference{
editor = "Lee, Bill, Gadow, Rainer, Mitić, Vojislav",
author = "Najdanović, Jelena and Najman, Stevo and Stojanović, Sanja and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Trajanović, Miroslav",
year = "2017",
abstract = "Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.",
publisher = "Paris : Atlantis Press",
journal = "Proceedings of the IV Advanced Ceramics and Applications Conference",
title = "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells",
pages = "387-400",
doi = "10.2991/978-94-6239-213-7_27",
url = "https://hdl.handle.net/21.15107/rcub_dais_2379"
}
Lee, B., Gadow, R., Mitić, V., Najdanović, J., Najman, S., Stojanović, S., Živković, J. M., Ignjatović, N., Uskoković, D.,& Trajanović, M.. (2017). The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference
Paris : Atlantis Press., 387-400.
https://doi.org/10.2991/978-94-6239-213-7_27
https://hdl.handle.net/21.15107/rcub_dais_2379
Lee B, Gadow R, Mitić V, Najdanović J, Najman S, Stojanović S, Živković JM, Ignjatović N, Uskoković D, Trajanović M. The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference. 2017;:387-400.
doi:10.2991/978-94-6239-213-7_27
https://hdl.handle.net/21.15107/rcub_dais_2379 .
Lee, Bill, Gadow, Rainer, Mitić, Vojislav, Najdanović, Jelena, Najman, Stevo, Stojanović, Sanja, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Trajanović, Miroslav, "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells" in Proceedings of the IV Advanced Ceramics and Applications Conference (2017):387-400,
https://doi.org/10.2991/978-94-6239-213-7_27 .,
https://hdl.handle.net/21.15107/rcub_dais_2379 .
1

Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review

Mitić, Žarko; Stolić, Aleksandra; Stojanović, Sanja; Najman, Stevo; Ignjatović, Nenad; Nikolić, Goran; Trajanović, Miroslav

(Elsevier, 2017)

TY  - JOUR
AU  - Mitić, Žarko
AU  - Stolić, Aleksandra
AU  - Stojanović, Sanja
AU  - Najman, Stevo
AU  - Ignjatović, Nenad
AU  - Nikolić, Goran
AU  - Trajanović, Miroslav
PY  - 2017
UR  - http://dais.sanu.ac.rs/123456789/2365
AB  - A review of recent advances in instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue is presented in this paper. In recent years, biomaterials attracted great attention primarily because of the wide range of biomedical applications. This paper focuses on the practical aspects of instrumental methods and techniques that were most often applied (X-ray methods, vibrational spectroscopy (IR and Raman), magnetic-resonance spectroscopy (NMR and ESR), mass spectrometry (MS), atomic absorption spectrometry (AAS) and inductively coupled plasma-atomic emission spectrometry (ICP-AES), thermogravimetry (TG), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), scanning electron microscopy (SEM), transmission electron microscopy (TEM)) in the structural investigation and physicochemical characterization of biomaterials and bone tissue. The application of some other physicochemical methods was also discussed. Hands-on information is provided about these valuable research tools, emphasizing practical aspects such as typical measurement conditions, their limitations and advantages, interpretation of results and practical applications.
PB  - Elsevier
T2  - Materials Science and Engineering: C
T1  - Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review
SP  - 930
EP  - 949
VL  - 79
DO  - 10.1016/j.msec.2017.05.127
UR  - https://hdl.handle.net/21.15107/rcub_dais_2365
ER  - 
@article{
author = "Mitić, Žarko and Stolić, Aleksandra and Stojanović, Sanja and Najman, Stevo and Ignjatović, Nenad and Nikolić, Goran and Trajanović, Miroslav",
year = "2017",
abstract = "A review of recent advances in instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue is presented in this paper. In recent years, biomaterials attracted great attention primarily because of the wide range of biomedical applications. This paper focuses on the practical aspects of instrumental methods and techniques that were most often applied (X-ray methods, vibrational spectroscopy (IR and Raman), magnetic-resonance spectroscopy (NMR and ESR), mass spectrometry (MS), atomic absorption spectrometry (AAS) and inductively coupled plasma-atomic emission spectrometry (ICP-AES), thermogravimetry (TG), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), scanning electron microscopy (SEM), transmission electron microscopy (TEM)) in the structural investigation and physicochemical characterization of biomaterials and bone tissue. The application of some other physicochemical methods was also discussed. Hands-on information is provided about these valuable research tools, emphasizing practical aspects such as typical measurement conditions, their limitations and advantages, interpretation of results and practical applications.",
publisher = "Elsevier",
journal = "Materials Science and Engineering: C",
title = "Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review",
pages = "930-949",
volume = "79",
doi = "10.1016/j.msec.2017.05.127",
url = "https://hdl.handle.net/21.15107/rcub_dais_2365"
}
Mitić, Ž., Stolić, A., Stojanović, S., Najman, S., Ignjatović, N., Nikolić, G.,& Trajanović, M.. (2017). Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review. in Materials Science and Engineering: C
Elsevier., 79, 930-949.
https://doi.org/10.1016/j.msec.2017.05.127
https://hdl.handle.net/21.15107/rcub_dais_2365
Mitić Ž, Stolić A, Stojanović S, Najman S, Ignjatović N, Nikolić G, Trajanović M. Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review. in Materials Science and Engineering: C. 2017;79:930-949.
doi:10.1016/j.msec.2017.05.127
https://hdl.handle.net/21.15107/rcub_dais_2365 .
Mitić, Žarko, Stolić, Aleksandra, Stojanović, Sanja, Najman, Stevo, Ignjatović, Nenad, Nikolić, Goran, Trajanović, Miroslav, "Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review" in Materials Science and Engineering: C, 79 (2017):930-949,
https://doi.org/10.1016/j.msec.2017.05.127 .,
https://hdl.handle.net/21.15107/rcub_dais_2365 .
45
37
41

In Vitro Evaluation of Nanoscale Hydroxyapatite-Based Bone Reconstructive Materials with Antimicrobial Properties

Ajduković, Zorica; Mihajilov Krstev, Tatjana; Ignjatović, Nenad; Stojanović, Zoran S.; Mladenović Antić, Snežana; Kocić, Branislava; Najman, Stevo; Petrović, Nenad D.; Uskoković, Dragan

(American Scientific Publishers, 2016)

TY  - JOUR
AU  - Ajduković, Zorica
AU  - Mihajilov Krstev, Tatjana
AU  - Ignjatović, Nenad
AU  - Stojanović, Zoran S.
AU  - Mladenović Antić, Snežana
AU  - Kocić, Branislava
AU  - Najman, Stevo
AU  - Petrović, Nenad D.
AU  - Uskoković, Dragan
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/2385
AB  - In the field of oral implantology the loss of bone tissue prevents adequate patient care, and calls for the use of synthetic biomaterials with properties that resemble natural bone. Special attention is paid to the risk of infection after the implantation of these materials. Studies have suggested that some nanocontructs containing metal ions have antimicrobial properties. The aim of this study was to examine the antimicrobial and hemolytic activity of cobalt-substituted hydroxyapatite nanoparticles, compared to hydroxyapatite and hydroxyapatite/poly-lactide-co-glycolide. The antibacterial effects of these powders were tested against two pathogenic bacterial strains: Escherichia coi (ATCC 25922) and Staphylococcus aureus (ATCC 25923), using the disc diffusion method and the quantitative antimicrobial test in a liquid medium. The quantitative antimicrobial test showed that all of the tested biomaterials have some antibacterial properties. The effects of both tests were more prominent in case of S. aureus than in E coli. A higher percentage of cobalt in the crystal structure of cobalt-substituted hydroxyapatite nanoparticles led to an increased antimicrobial activity. All of the presented biomaterial samples were found to be non-hemolytic. Having in mind that the tested of cobalt-substituted hydroxyapatite (Ca/Co-HAp) material in given concentrations shows good hemocompatibility and antimicrobial effects, along with its previously studied biological properties, the conclusion can be reached that it is a potential candidate that could substitute calcium hydroxyapatite as the material of choice for use in bone tissue engineering and clinical practices in orthopedic, oral and maxillofacial surgery.
PB  - American Scientific Publishers
T2  - Journal of Nanoscience and Nanotechnology
T1  - In Vitro Evaluation of Nanoscale Hydroxyapatite-Based Bone Reconstructive Materials with Antimicrobial Properties
SP  - 1420
EP  - 1428
VL  - 16
IS  - 2
DO  - 10.1166/jnn.2016.10699
UR  - https://hdl.handle.net/21.15107/rcub_dais_2385
ER  - 
@article{
author = "Ajduković, Zorica and Mihajilov Krstev, Tatjana and Ignjatović, Nenad and Stojanović, Zoran S. and Mladenović Antić, Snežana and Kocić, Branislava and Najman, Stevo and Petrović, Nenad D. and Uskoković, Dragan",
year = "2016",
abstract = "In the field of oral implantology the loss of bone tissue prevents adequate patient care, and calls for the use of synthetic biomaterials with properties that resemble natural bone. Special attention is paid to the risk of infection after the implantation of these materials. Studies have suggested that some nanocontructs containing metal ions have antimicrobial properties. The aim of this study was to examine the antimicrobial and hemolytic activity of cobalt-substituted hydroxyapatite nanoparticles, compared to hydroxyapatite and hydroxyapatite/poly-lactide-co-glycolide. The antibacterial effects of these powders were tested against two pathogenic bacterial strains: Escherichia coi (ATCC 25922) and Staphylococcus aureus (ATCC 25923), using the disc diffusion method and the quantitative antimicrobial test in a liquid medium. The quantitative antimicrobial test showed that all of the tested biomaterials have some antibacterial properties. The effects of both tests were more prominent in case of S. aureus than in E coli. A higher percentage of cobalt in the crystal structure of cobalt-substituted hydroxyapatite nanoparticles led to an increased antimicrobial activity. All of the presented biomaterial samples were found to be non-hemolytic. Having in mind that the tested of cobalt-substituted hydroxyapatite (Ca/Co-HAp) material in given concentrations shows good hemocompatibility and antimicrobial effects, along with its previously studied biological properties, the conclusion can be reached that it is a potential candidate that could substitute calcium hydroxyapatite as the material of choice for use in bone tissue engineering and clinical practices in orthopedic, oral and maxillofacial surgery.",
publisher = "American Scientific Publishers",
journal = "Journal of Nanoscience and Nanotechnology",
title = "In Vitro Evaluation of Nanoscale Hydroxyapatite-Based Bone Reconstructive Materials with Antimicrobial Properties",
pages = "1420-1428",
volume = "16",
number = "2",
doi = "10.1166/jnn.2016.10699",
url = "https://hdl.handle.net/21.15107/rcub_dais_2385"
}
Ajduković, Z., Mihajilov Krstev, T., Ignjatović, N., Stojanović, Z. S., Mladenović Antić, S., Kocić, B., Najman, S., Petrović, N. D.,& Uskoković, D.. (2016). In Vitro Evaluation of Nanoscale Hydroxyapatite-Based Bone Reconstructive Materials with Antimicrobial Properties. in Journal of Nanoscience and Nanotechnology
American Scientific Publishers., 16(2), 1420-1428.
https://doi.org/10.1166/jnn.2016.10699
https://hdl.handle.net/21.15107/rcub_dais_2385
Ajduković Z, Mihajilov Krstev T, Ignjatović N, Stojanović ZS, Mladenović Antić S, Kocić B, Najman S, Petrović ND, Uskoković D. In Vitro Evaluation of Nanoscale Hydroxyapatite-Based Bone Reconstructive Materials with Antimicrobial Properties. in Journal of Nanoscience and Nanotechnology. 2016;16(2):1420-1428.
doi:10.1166/jnn.2016.10699
https://hdl.handle.net/21.15107/rcub_dais_2385 .
Ajduković, Zorica, Mihajilov Krstev, Tatjana, Ignjatović, Nenad, Stojanović, Zoran S., Mladenović Antić, Snežana, Kocić, Branislava, Najman, Stevo, Petrović, Nenad D., Uskoković, Dragan, "In Vitro Evaluation of Nanoscale Hydroxyapatite-Based Bone Reconstructive Materials with Antimicrobial Properties" in Journal of Nanoscience and Nanotechnology, 16, no. 2 (2016):1420-1428,
https://doi.org/10.1166/jnn.2016.10699 .,
https://hdl.handle.net/21.15107/rcub_dais_2385 .
3
3
4

Scanning electron microscopy analysis of changes of hydroxiapatite/poly-l-lactide with different molecular weight of PLLA after intraperitoneal implantation

Đorđević, Ljubiša; Najman, Stevo; Vasiljević, Perica; Miljković, Miroslav; Ignjatović, Nenad; Uskoković, Dragan; Plavšić, Milenko

(2016)

TY  - JOUR
AU  - Đorđević, Ljubiša
AU  - Najman, Stevo
AU  - Vasiljević, Perica
AU  - Miljković, Miroslav
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Plavšić, Milenko
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/15457
AB  - Implantation of a biomaterial is one of the important trends in solving the problem of bone tissue loss. Calcium hydroxiapatite (HAp), as the most representative bone component is a serious candidate for such implantations. The synthetic polymer poly-L-lactide (PLLA) in HAp/PLLA is often used as a polymeric material, with a role in the substitution of bone tissue collagen fibers. Fibers of PLLA may strengthen HAp and its good bioresorption provides space for tissue remodeling. Differences in porosity, microstructure, compressive consistency as well as bioresorbility of HAp/PLLA may be achieved by using PLLA with different molecular weights. In this study HAp/PLLA composites with PLLA of different molecular weights (50,000; 160,000 and 430,000) were implanted in mouse peritoneum in order to examine the influence of the molecular weight of PLLA on morphology changes. Microstructural changes of biomaterial (HAp/PLLA) surface were analyzed one week, three weeks and four months after their implantation using Scanning Electron Microscopy. The results showed a significant difference in tissue reactions on the applied biocomposites, depending on their molecular weight. The most intense proliferation of cells was induced by HAp/PLLA 50,000 compared to HAp/PLLA 430,000 and HAp/PLLA 160,000. In the vicinity of HAp/PLLA 430,000 abundant erythrocytes were observed. The differences in biological reactions on the examined biocomposites are significant for their practical applications. HAp/PLLA composite biomaterials of different types and resorption rates require specific designing and programming to become suitable for particular purposes in an organism.
T2  - Acta Veterinaria
T1  - Scanning electron microscopy analysis of changes of hydroxiapatite/poly-l-lactide with different molecular weight of PLLA after intraperitoneal implantation
SP  - 234
EP  - 244
VL  - 66
IS  - 2
DO  - 10.1515/acve-2016-0020
UR  - https://hdl.handle.net/21.15107/rcub_dais_15457
ER  - 
@article{
author = "Đorđević, Ljubiša and Najman, Stevo and Vasiljević, Perica and Miljković, Miroslav and Ignjatović, Nenad and Uskoković, Dragan and Plavšić, Milenko",
year = "2016",
abstract = "Implantation of a biomaterial is one of the important trends in solving the problem of bone tissue loss. Calcium hydroxiapatite (HAp), as the most representative bone component is a serious candidate for such implantations. The synthetic polymer poly-L-lactide (PLLA) in HAp/PLLA is often used as a polymeric material, with a role in the substitution of bone tissue collagen fibers. Fibers of PLLA may strengthen HAp and its good bioresorption provides space for tissue remodeling. Differences in porosity, microstructure, compressive consistency as well as bioresorbility of HAp/PLLA may be achieved by using PLLA with different molecular weights. In this study HAp/PLLA composites with PLLA of different molecular weights (50,000; 160,000 and 430,000) were implanted in mouse peritoneum in order to examine the influence of the molecular weight of PLLA on morphology changes. Microstructural changes of biomaterial (HAp/PLLA) surface were analyzed one week, three weeks and four months after their implantation using Scanning Electron Microscopy. The results showed a significant difference in tissue reactions on the applied biocomposites, depending on their molecular weight. The most intense proliferation of cells was induced by HAp/PLLA 50,000 compared to HAp/PLLA 430,000 and HAp/PLLA 160,000. In the vicinity of HAp/PLLA 430,000 abundant erythrocytes were observed. The differences in biological reactions on the examined biocomposites are significant for their practical applications. HAp/PLLA composite biomaterials of different types and resorption rates require specific designing and programming to become suitable for particular purposes in an organism.",
journal = "Acta Veterinaria",
title = "Scanning electron microscopy analysis of changes of hydroxiapatite/poly-l-lactide with different molecular weight of PLLA after intraperitoneal implantation",
pages = "234-244",
volume = "66",
number = "2",
doi = "10.1515/acve-2016-0020",
url = "https://hdl.handle.net/21.15107/rcub_dais_15457"
}
Đorđević, L., Najman, S., Vasiljević, P., Miljković, M., Ignjatović, N., Uskoković, D.,& Plavšić, M.. (2016). Scanning electron microscopy analysis of changes of hydroxiapatite/poly-l-lactide with different molecular weight of PLLA after intraperitoneal implantation. in Acta Veterinaria, 66(2), 234-244.
https://doi.org/10.1515/acve-2016-0020
https://hdl.handle.net/21.15107/rcub_dais_15457
Đorđević L, Najman S, Vasiljević P, Miljković M, Ignjatović N, Uskoković D, Plavšić M. Scanning electron microscopy analysis of changes of hydroxiapatite/poly-l-lactide with different molecular weight of PLLA after intraperitoneal implantation. in Acta Veterinaria. 2016;66(2):234-244.
doi:10.1515/acve-2016-0020
https://hdl.handle.net/21.15107/rcub_dais_15457 .
Đorđević, Ljubiša, Najman, Stevo, Vasiljević, Perica, Miljković, Miroslav, Ignjatović, Nenad, Uskoković, Dragan, Plavšić, Milenko, "Scanning electron microscopy analysis of changes of hydroxiapatite/poly-l-lactide with different molecular weight of PLLA after intraperitoneal implantation" in Acta Veterinaria, 66, no. 2 (2016):234-244,
https://doi.org/10.1515/acve-2016-0020 .,
https://hdl.handle.net/21.15107/rcub_dais_15457 .

Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period

Mitić, Žarko; Najman, Stevo; Cakić, Milorad; Ajduković, Zorica; Ignjatović, Nenad; Nikolić, Ružica; Nikolić, Goran; Stojanović, Sanja; Vukelić, Marija Đ.; Trajanović, Miroslav

(Elsevier, 2014)

TY  - JOUR
AU  - Mitić, Žarko
AU  - Najman, Stevo
AU  - Cakić, Milorad
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Nikolić, Ružica
AU  - Nikolić, Goran
AU  - Stojanović, Sanja
AU  - Vukelić, Marija Đ.
AU  - Trajanović, Miroslav
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/554
AB  - The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period
SP  - 315
EP  - 320
VL  - 1074
DO  - 10.1016/j.molstruc.2014.06.006
UR  - https://hdl.handle.net/21.15107/rcub_dais_554
ER  - 
@article{
author = "Mitić, Žarko and Najman, Stevo and Cakić, Milorad and Ajduković, Zorica and Ignjatović, Nenad and Nikolić, Ružica and Nikolić, Goran and Stojanović, Sanja and Vukelić, Marija Đ. and Trajanović, Miroslav",
year = "2014",
abstract = "The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period",
pages = "315-320",
volume = "1074",
doi = "10.1016/j.molstruc.2014.06.006",
url = "https://hdl.handle.net/21.15107/rcub_dais_554"
}
Mitić, Ž., Najman, S., Cakić, M., Ajduković, Z., Ignjatović, N., Nikolić, R., Nikolić, G., Stojanović, S., Vukelić, M. Đ.,& Trajanović, M.. (2014). Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. in Journal of Molecular Structure
Elsevier., 1074, 315-320.
https://doi.org/10.1016/j.molstruc.2014.06.006
https://hdl.handle.net/21.15107/rcub_dais_554
Mitić Ž, Najman S, Cakić M, Ajduković Z, Ignjatović N, Nikolić R, Nikolić G, Stojanović S, Vukelić MĐ, Trajanović M. Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. in Journal of Molecular Structure. 2014;1074:315-320.
doi:10.1016/j.molstruc.2014.06.006
https://hdl.handle.net/21.15107/rcub_dais_554 .
Mitić, Žarko, Najman, Stevo, Cakić, Milorad, Ajduković, Zorica, Ignjatović, Nenad, Nikolić, Ružica, Nikolić, Goran, Stojanović, Sanja, Vukelić, Marija Đ., Trajanović, Miroslav, "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period" in Journal of Molecular Structure, 1074 (2014):315-320,
https://doi.org/10.1016/j.molstruc.2014.06.006 .,
https://hdl.handle.net/21.15107/rcub_dais_554 .
5
2
5

Interaction of nanoparticles and biological fluids

Ajduković, Zorica; Ignjatović, Nenad; Petrović, Nenad; Rajković, Jelena; Kenić Marinković, Dragana; Najman, Stevo; Mihailović, Dragan; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2014)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Petrović, Nenad
AU  - Rajković, Jelena
AU  - Kenić Marinković, Dragana
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/558
AB  - Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomolecules such are proteins, adsorbs these molecules, and leads to formation of nanoparticle-biomoelcular complex known as “protein corona”. This protein corona changes the properties of nanoparticles and their behavior in vivo. In this work three types of nanomaterial based on Hap and polymers were incubated in rats plasma, and the identification and quantification of proteins in protein corona that had formed around these nanoparticles, was done by means of electrophoresis and mass spectrophotometery.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
T1  - Interaction of nanoparticles and biological fluids
SP  - 113
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_dais_558
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad and Petrović, Nenad and Rajković, Jelena and Kenić Marinković, Dragana and Najman, Stevo and Mihailović, Dragan and Uskoković, Dragan",
year = "2014",
abstract = "Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomolecules such are proteins, adsorbs these molecules, and leads to formation of nanoparticle-biomoelcular complex known as “protein corona”. This protein corona changes the properties of nanoparticles and their behavior in vivo. In this work three types of nanomaterial based on Hap and polymers were incubated in rats plasma, and the identification and quantification of proteins in protein corona that had formed around these nanoparticles, was done by means of electrophoresis and mass spectrophotometery.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts",
title = "Interaction of nanoparticles and biological fluids",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_dais_558"
}
Ajduković, Z., Ignjatović, N., Petrović, N., Rajković, J., Kenić Marinković, D., Najman, S., Mihailović, D.,& Uskoković, D.. (2014). Interaction of nanoparticles and biological fluids. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 113-113.
https://hdl.handle.net/21.15107/rcub_dais_558
Ajduković Z, Ignjatović N, Petrović N, Rajković J, Kenić Marinković D, Najman S, Mihailović D, Uskoković D. Interaction of nanoparticles and biological fluids. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:113-113.
https://hdl.handle.net/21.15107/rcub_dais_558 .
Ajduković, Zorica, Ignjatović, Nenad, Petrović, Nenad, Rajković, Jelena, Kenić Marinković, Dragana, Najman, Stevo, Mihailović, Dragan, Uskoković, Dragan, "Interaction of nanoparticles and biological fluids" in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts (2014):113-113,
https://hdl.handle.net/21.15107/rcub_dais_558 .

Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta

Ajduković, Zorica; Ignjatović, Nenad; Petrović, N.; Rajković, J.; Kenić Marinković, Dragana; Najman, Stevo; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2013)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Petrović, N.
AU  - Rajković, J.
AU  - Kenić Marinković, Dragana
AU  - Najman, Stevo
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/388
AB  - The lack of bone in the jaw tegmenta inflicts major problem and leads to an inability to adequately treat stomatoprosthetic patients. If the bone tissue damage is minor, the balanced activities of osteoblasts and bone osteoclasts can repair it independently. If the damage is bigger it is necessary to support the biological potential to repair the bone, and for that reason nanoparticle
biomaterial Ca / Co-HAp was used in this study. The research was done on rats with uniform anatomical and physiological characteristics. Assessment of repair and consolidation of the jaw bone tegmenta was performed by istomorphometric and SEM analysis. The best results were obtained in the experimental group of animals where the Ca / Co-Hap was mixed with autologous
plasma. Following the implementation of the above mentioned nanocomposites, a significant formation of new bone was evident on the SEM analysis, as well as the rising of histomorphometric parameters of bone formation, which indicates that the Ca / Co-HAp nanocomposite is the material of choice for the rapid regeneration and repair of bone jaw tegmenta.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta
SP  - 147
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_dais_388
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad and Petrović, N. and Rajković, J. and Kenić Marinković, Dragana and Najman, Stevo and Uskoković, Dragan",
year = "2013",
abstract = "The lack of bone in the jaw tegmenta inflicts major problem and leads to an inability to adequately treat stomatoprosthetic patients. If the bone tissue damage is minor, the balanced activities of osteoblasts and bone osteoclasts can repair it independently. If the damage is bigger it is necessary to support the biological potential to repair the bone, and for that reason nanoparticle
biomaterial Ca / Co-HAp was used in this study. The research was done on rats with uniform anatomical and physiological characteristics. Assessment of repair and consolidation of the jaw bone tegmenta was performed by istomorphometric and SEM analysis. The best results were obtained in the experimental group of animals where the Ca / Co-Hap was mixed with autologous
plasma. Following the implementation of the above mentioned nanocomposites, a significant formation of new bone was evident on the SEM analysis, as well as the rising of histomorphometric parameters of bone formation, which indicates that the Ca / Co-HAp nanocomposite is the material of choice for the rapid regeneration and repair of bone jaw tegmenta.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_dais_388"
}
Ajduković, Z., Ignjatović, N., Petrović, N., Rajković, J., Kenić Marinković, D., Najman, S.,& Uskoković, D.. (2013). Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 147-147.
https://hdl.handle.net/21.15107/rcub_dais_388
Ajduković Z, Ignjatović N, Petrović N, Rajković J, Kenić Marinković D, Najman S, Uskoković D. Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:147-147.
https://hdl.handle.net/21.15107/rcub_dais_388 .
Ajduković, Zorica, Ignjatović, Nenad, Petrović, N., Rajković, J., Kenić Marinković, Dragana, Najman, Stevo, Uskoković, Dragan, "Nanoparticles Ca/Co-HAp in the treatment of weakened bones jaw tegmenta" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):147-147,
https://hdl.handle.net/21.15107/rcub_dais_388 .

Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles

Najman, Stevo; Petrović, Dragan; Vučković, I.; Živković, Jelena M.; Stojanović, S.; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2013)

TY  - CONF
AU  - Najman, Stevo
AU  - Petrović, Dragan
AU  - Vučković, I.
AU  - Živković, Jelena M.
AU  - Stojanović, S.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/400
AB  - The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles
SP  - 45
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_dais_400
ER  - 
@conference{
author = "Najman, Stevo and Petrović, Dragan and Vučković, I. and Živković, Jelena M. and Stojanović, S. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2013",
abstract = "The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles",
pages = "45-45",
url = "https://hdl.handle.net/21.15107/rcub_dais_400"
}
Najman, S., Petrović, D., Vučković, I., Živković, J. M., Stojanović, S., Ignjatović, N.,& Uskoković, D.. (2013). Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 45-45.
https://hdl.handle.net/21.15107/rcub_dais_400
Najman S, Petrović D, Vučković I, Živković JM, Stojanović S, Ignjatović N, Uskoković D. Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:45-45.
https://hdl.handle.net/21.15107/rcub_dais_400 .
Najman, Stevo, Petrović, Dragan, Vučković, I., Živković, Jelena M., Stojanović, S., Ignjatović, Nenad, Uskoković, Dragan, "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):45-45,
https://hdl.handle.net/21.15107/rcub_dais_400 .

Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions

Stojanović, S.; Mitić, Ž.; Najman, Stevo; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2013)

TY  - CONF
AU  - Stojanović, S.
AU  - Mitić, Ž.
AU  - Najman, Stevo
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/406
AB  - In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions
SP  - 149
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_dais_406
ER  - 
@conference{
author = "Stojanović, S. and Mitić, Ž. and Najman, Stevo and Ignjatović, Nenad and Uskoković, Dragan",
year = "2013",
abstract = "In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions",
pages = "149-149",
url = "https://hdl.handle.net/21.15107/rcub_dais_406"
}
Stojanović, S., Mitić, Ž., Najman, S., Ignjatović, N.,& Uskoković, D.. (2013). Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 149-149.
https://hdl.handle.net/21.15107/rcub_dais_406
Stojanović S, Mitić Ž, Najman S, Ignjatović N, Uskoković D. Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:149-149.
https://hdl.handle.net/21.15107/rcub_dais_406 .
Stojanović, S., Mitić, Ž., Najman, Stevo, Ignjatović, Nenad, Uskoković, Dragan, "Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):149-149,
https://hdl.handle.net/21.15107/rcub_dais_406 .

Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells

Najman, Stevo; Stojanović, Sanja; Mitrić, Žarko; Ajduković, Zorica; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Mitrić, Žarko
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/519
AB  - We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_dais_519
ER  - 
@conference{
author = "Najman, Stevo and Stojanović, Sanja and Mitrić, Žarko and Ajduković, Zorica and Ignjatović, Nenad and Uskoković, Dragan",
year = "2012",
abstract = "We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_dais_519"
}
Najman, S., Stojanović, S., Mitrić, Ž., Ajduković, Z., Ignjatović, N.,& Uskoković, D.. (2012). Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 110-110.
https://hdl.handle.net/21.15107/rcub_dais_519
Najman S, Stojanović S, Mitrić Ž, Ajduković Z, Ignjatović N, Uskoković D. Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:110-110.
https://hdl.handle.net/21.15107/rcub_dais_519 .
Najman, Stevo, Stojanović, Sanja, Mitrić, Žarko, Ajduković, Zorica, Ignjatović, Nenad, Uskoković, Dragan, "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells" in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):110-110,
https://hdl.handle.net/21.15107/rcub_dais_519 .

Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid

Vukelić, Marija Đ.; Mitić, Žarko; Miljković, Miroslav; Živković, Jelena M.; Ignjatović, Nenad; Uskoković, Dragan; Živanov Čurlis, Jelena Z.; Vasiljević, Perica; Najman, Stevo

(Milano : Wichtig Publishing, 2012)

TY  - JOUR
AU  - Vukelić, Marija Đ.
AU  - Mitić, Žarko
AU  - Miljković, Miroslav
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Živanov Čurlis, Jelena Z.
AU  - Vasiljević, Perica
AU  - Najman, Stevo
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/489
AB  - Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.
PB  - Milano : Wichtig Publishing
T2  - Journal of Applied Biomaterials and Functional Materials
T1  - Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid
SP  - 43
EP  - 48
DO  - 10.5301/JABFM.2012.9274
UR  - https://hdl.handle.net/21.15107/rcub_dais_489
ER  - 
@article{
author = "Vukelić, Marija Đ. and Mitić, Žarko and Miljković, Miroslav and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Živanov Čurlis, Jelena Z. and Vasiljević, Perica and Najman, Stevo",
year = "2012",
abstract = "Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.",
publisher = "Milano : Wichtig Publishing",
journal = "Journal of Applied Biomaterials and Functional Materials",
title = "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid",
pages = "43-48",
doi = "10.5301/JABFM.2012.9274",
url = "https://hdl.handle.net/21.15107/rcub_dais_489"
}
Vukelić, M. Đ., Mitić, Ž., Miljković, M., Živković, J. M., Ignjatović, N., Uskoković, D., Živanov Čurlis, J. Z., Vasiljević, P.,& Najman, S.. (2012). Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials
Milano : Wichtig Publishing., 43-48.
https://doi.org/10.5301/JABFM.2012.9274
https://hdl.handle.net/21.15107/rcub_dais_489
Vukelić MĐ, Mitić Ž, Miljković M, Živković JM, Ignjatović N, Uskoković D, Živanov Čurlis JZ, Vasiljević P, Najman S. Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials. 2012;:43-48.
doi:10.5301/JABFM.2012.9274
https://hdl.handle.net/21.15107/rcub_dais_489 .
Vukelić, Marija Đ., Mitić, Žarko, Miljković, Miroslav, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Živanov Čurlis, Jelena Z., Vasiljević, Perica, Najman, Stevo, "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid" in Journal of Applied Biomaterials and Functional Materials (2012):43-48,
https://doi.org/10.5301/JABFM.2012.9274 .,
https://hdl.handle.net/21.15107/rcub_dais_489 .
7
6
9

Hemolytic activity of bioactive nanocomposites

Ajduković, Zorica; Ignjatović, Nenad; Petrović, N.; Najman, Stevo; Rajković, J.; Kenić Marinković, Dragana; Krstić, V.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Petrović, N.
AU  - Najman, Stevo
AU  - Rajković, J.
AU  - Kenić Marinković, Dragana
AU  - Krstić, V.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/497
AB  - Huge range of tested biomaterials in recent decades has emerged as an ideal scaffold for cell growth, but few have demonstrated clinical efficacy. Among them, synthetic hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is the most promising because of its biocompatibility, bioactivity, and osteoconductivity. Biocompatibility represents the primary concern for any material to be used as a substitute for natural tissue. Hydroxyapatite particles interact with numerous cellular systems in vivo, and some of these interactions may lead to cell damage and to stimulate platelet activation, coagulation and thrombus formation. The aim of this work was to examine the hemocompatibility of nanocalcium hydroxyapatite substituted with 5% and 12% cobalt (Ca /CoHAp) and hydroxyapatite/polylactidcoglicolid (HAp / PLGA) in relation to pure HAp by testing their hemolytic activities. The results show the discrepancy in hemolytic activity of implanted matherials. The degree of crystallinity of samples had a more dominant influence on hemolysis than the percentage of substituted cobalt. Hemolysis ratios of the nanocalcium hydroxyapatite substituted with cobalt samples were below 3%, indicating good blood compatibility and that they are promising for medical application.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Hemolytic activity of bioactive nanocomposites
SP  - 49
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_dais_497
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad and Petrović, N. and Najman, Stevo and Rajković, J. and Kenić Marinković, Dragana and Krstić, V. and Uskoković, Dragan",
year = "2012",
abstract = "Huge range of tested biomaterials in recent decades has emerged as an ideal scaffold for cell growth, but few have demonstrated clinical efficacy. Among them, synthetic hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is the most promising because of its biocompatibility, bioactivity, and osteoconductivity. Biocompatibility represents the primary concern for any material to be used as a substitute for natural tissue. Hydroxyapatite particles interact with numerous cellular systems in vivo, and some of these interactions may lead to cell damage and to stimulate platelet activation, coagulation and thrombus formation. The aim of this work was to examine the hemocompatibility of nanocalcium hydroxyapatite substituted with 5% and 12% cobalt (Ca /CoHAp) and hydroxyapatite/polylactidcoglicolid (HAp / PLGA) in relation to pure HAp by testing their hemolytic activities. The results show the discrepancy in hemolytic activity of implanted matherials. The degree of crystallinity of samples had a more dominant influence on hemolysis than the percentage of substituted cobalt. Hemolysis ratios of the nanocalcium hydroxyapatite substituted with cobalt samples were below 3%, indicating good blood compatibility and that they are promising for medical application.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Hemolytic activity of bioactive nanocomposites",
pages = "49-49",
url = "https://hdl.handle.net/21.15107/rcub_dais_497"
}
Ajduković, Z., Ignjatović, N., Petrović, N., Najman, S., Rajković, J., Kenić Marinković, D., Krstić, V.,& Uskoković, D.. (2012). Hemolytic activity of bioactive nanocomposites. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 49-49.
https://hdl.handle.net/21.15107/rcub_dais_497
Ajduković Z, Ignjatović N, Petrović N, Najman S, Rajković J, Kenić Marinković D, Krstić V, Uskoković D. Hemolytic activity of bioactive nanocomposites. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:49-49.
https://hdl.handle.net/21.15107/rcub_dais_497 .
Ajduković, Zorica, Ignjatović, Nenad, Petrović, N., Najman, Stevo, Rajković, J., Kenić Marinković, Dragana, Krstić, V., Uskoković, Dragan, "Hemolytic activity of bioactive nanocomposites" in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):49-49,
https://hdl.handle.net/21.15107/rcub_dais_497 .

Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges

Ajduković, Zorica; Ignjatović, Nenad; Rajković, Jelena; Najman, Stevo; Mihailović, Dragan; Petrović, D.; Kenić Marinković, Dragana; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Rajković, Jelena
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Petrović, D.
AU  - Kenić Marinković, Dragana
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/498
AB  - Bone loss during the systemic osteoporosis has an important role in dentistry and medicine. The aim of the study was the application of bioactive micro and nanocomposite materials, alone, and in combination with autologous plasma in osteoporotic jaw bones of rats with artificially induced osteoporosis. The effect of these composites was measured by histomorphometric and atomic absorption spectrophotometric analysis. According to the best obtained results in regeneration and recovery of the resorbed alveolar bone, it can be concluded that nanocomposite combined with autologous plasma may be the material of choice to replace the osteoporotic damaged jaw bone.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges
SP  - 103
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_dais_498
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad and Rajković, Jelena and Najman, Stevo and Mihailović, Dragan and Petrović, D. and Kenić Marinković, Dragana and Uskoković, Dragan",
year = "2012",
abstract = "Bone loss during the systemic osteoporosis has an important role in dentistry and medicine. The aim of the study was the application of bioactive micro and nanocomposite materials, alone, and in combination with autologous plasma in osteoporotic jaw bones of rats with artificially induced osteoporosis. The effect of these composites was measured by histomorphometric and atomic absorption spectrophotometric analysis. According to the best obtained results in regeneration and recovery of the resorbed alveolar bone, it can be concluded that nanocomposite combined with autologous plasma may be the material of choice to replace the osteoporotic damaged jaw bone.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges",
pages = "103-103",
url = "https://hdl.handle.net/21.15107/rcub_dais_498"
}
Ajduković, Z., Ignjatović, N., Rajković, J., Najman, S., Mihailović, D., Petrović, D., Kenić Marinković, D.,& Uskoković, D.. (2012). Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 103-103.
https://hdl.handle.net/21.15107/rcub_dais_498
Ajduković Z, Ignjatović N, Rajković J, Najman S, Mihailović D, Petrović D, Kenić Marinković D, Uskoković D. Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:103-103.
https://hdl.handle.net/21.15107/rcub_dais_498 .
Ajduković, Zorica, Ignjatović, Nenad, Rajković, Jelena, Najman, Stevo, Mihailović, Dragan, Petrović, D., Kenić Marinković, Dragana, Uskoković, Dragan, "Bioactive composite materials in regeneration of the resorbed bone of alveolar ridges" in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):103-103,
https://hdl.handle.net/21.15107/rcub_dais_498 .

Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta

Ajduković, Zorica; Ignjatović, Nenad; Savić, Vojin; Najman, Stevo; Mihailović, Dragan; Rajković, J.; Petrović, N.; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Rajković, J.
AU  - Petrović, N.
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/455
AB  - In recent years, calcium hydroxyapatite (HAp) and its substituents are increasingly used in dentistry and medicine. The influence of nanoparticles Ca / Co-HAp in strengthening weak osteoporotic bone jaw tegmenta was tested in an experimental model. The study was conducted on Wistar soy rats, aged 6-8 weeks. The biomaterial was implanted in the osteoporoticly weakened mandible of these animals. The best results in the strengthening of the lower jaw bone tegmenta were achieved 24 weeks after implantation of hydroxyapatite nanoparticles in which the calcium ion was substituted with 12% of cobalt ions. Histochemical parameters of bone synthesis were in a statistically significant increase. SEM analysis showed a high degree of osteogenetic ability of nano-particulate material implanted in the bone defect.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta
SP  - 121
EP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_dais_455
ER  - 
@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Rajković, J. and Petrović, N. and Uskoković, Dragan",
year = "2012",
abstract = "In recent years, calcium hydroxyapatite (HAp) and its substituents are increasingly used in dentistry and medicine. The influence of nanoparticles Ca / Co-HAp in strengthening weak osteoporotic bone jaw tegmenta was tested in an experimental model. The study was conducted on Wistar soy rats, aged 6-8 weeks. The biomaterial was implanted in the osteoporoticly weakened mandible of these animals. The best results in the strengthening of the lower jaw bone tegmenta were achieved 24 weeks after implantation of hydroxyapatite nanoparticles in which the calcium ion was substituted with 12% of cobalt ions. Histochemical parameters of bone synthesis were in a statistically significant increase. SEM analysis showed a high degree of osteogenetic ability of nano-particulate material implanted in the bone defect.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_dais_455"
}
Ajduković, Z., Ignjatović, N., Savić, V., Najman, S., Mihailović, D., Rajković, J., Petrović, N.,& Uskoković, D.. (2012). Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 121-121.
https://hdl.handle.net/21.15107/rcub_dais_455
Ajduković Z, Ignjatović N, Savić V, Najman S, Mihailović D, Rajković J, Petrović N, Uskoković D. Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:121-121.
https://hdl.handle.net/21.15107/rcub_dais_455 .
Ajduković, Zorica, Ignjatović, Nenad, Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Rajković, J., Petrović, N., Uskoković, Dragan, "Hydroxyapatite and hydroxyapatite substituents in strengthening of the jaw bone tegmenta" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):121-121,
https://hdl.handle.net/21.15107/rcub_dais_455 .

Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells

Stojanović, S.; Najman, Stevo; Ajduković, Zorica; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Stojanović, S.
AU  - Najman, Stevo
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/458
AB  - The aim of this study was to compare the effects of two types of nanomaterials, cobalt-exchanged hydroxyapatite (CoHAp) and calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA), on the viability of Saos-2 osteoblast-like cells, using MTT test. We have examined the suspension of this materials in the following concentrations: 1.6, 8, 40, 200, 1000 and 2500 μg/ml, as well as extracts in concentration range from 2,5% to 100%. Both materials showed cytotoxic effect at higher concentrations of suspension and extract, respectively, but they were not cytotoxic at lower concentrations. CP/PLGA acted stronger cytotoxic compared to CoHAp, regardless of whether it is examined suspension or extract. CoHAp in small concentrations of suspension and extract acted slightly stimulatory on cells. This suggests that CoHAp may have advantage for use in the in vivo systems.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells
SP  - 126
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_dais_458
ER  - 
@conference{
author = "Stojanović, S. and Najman, Stevo and Ajduković, Zorica and Ignjatović, Nenad and Uskoković, Dragan",
year = "2012",
abstract = "The aim of this study was to compare the effects of two types of nanomaterials, cobalt-exchanged hydroxyapatite (CoHAp) and calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA), on the viability of Saos-2 osteoblast-like cells, using MTT test. We have examined the suspension of this materials in the following concentrations: 1.6, 8, 40, 200, 1000 and 2500 μg/ml, as well as extracts in concentration range from 2,5% to 100%. Both materials showed cytotoxic effect at higher concentrations of suspension and extract, respectively, but they were not cytotoxic at lower concentrations. CP/PLGA acted stronger cytotoxic compared to CoHAp, regardless of whether it is examined suspension or extract. CoHAp in small concentrations of suspension and extract acted slightly stimulatory on cells. This suggests that CoHAp may have advantage for use in the in vivo systems.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_dais_458"
}
Stojanović, S., Najman, S., Ajduković, Z., Ignjatović, N.,& Uskoković, D.. (2012). Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 126-126.
https://hdl.handle.net/21.15107/rcub_dais_458
Stojanović S, Najman S, Ajduković Z, Ignjatović N, Uskoković D. Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:126-126.
https://hdl.handle.net/21.15107/rcub_dais_458 .
Stojanović, S., Najman, Stevo, Ajduković, Zorica, Ignjatović, Nenad, Uskoković, Dragan, "Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):126-126,
https://hdl.handle.net/21.15107/rcub_dais_458 .

Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density

Ajduković, Zorica; Petrović, Milica B.; Milićević, Jelena; Đorđević, Nadica; Ignjatović, Nenad; Savić, Vojin; Najman, Stevo; Mihailović, Dragan; Vasiljević, Perica; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2011)

TY  - CONF
AU  - Ajduković, Zorica
AU  - Petrović, Milica B.
AU  - Milićević, Jelena
AU  - Đorđević, Nadica
AU  - Ignjatović, Nenad
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, Perica
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/674
AB  - One of the trends in the treatment of reduced bone density is in oral implantology through the use of new biomaterials with paramagnetic properties. In this in vivo study the effect of Ca /Co-HAp nanoparticles (calcium/cobalt-hydroxiapatite) in the regeneration of the reduced alveolar bone density in experimental animals was examined by analyzing both the biochemical and histochemical markers and through analysis of alveolar bone density. The research was carried out on female Westar rats, aged 6-8 weeks, whom have been implanted nanobiomaterial in reduced alveolar bone density. Good results in regeneration of reduced bone density were achieved in six weeks after implantation of the nanoparticles with paramagnetic properties. Biochemical markers of osteogenesis showed statistically significant rise. Histological analysis revealed high level reparatory skills of the biocomposite implanted in the bone defect, while X-ray analyses evidenced rise of bone density. Implantation of Ca/Co-Hap enables rapid new formation, thus becoming the material of choice for accelerated reduced bone density regeneration.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
T1  - Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density
SP  - 161
EP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_dais_674
ER  - 
@conference{
author = "Ajduković, Zorica and Petrović, Milica B. and Milićević, Jelena and Đorđević, Nadica and Ignjatović, Nenad and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Uskoković, Dragan",
year = "2011",
abstract = "One of the trends in the treatment of reduced bone density is in oral implantology through the use of new biomaterials with paramagnetic properties. In this in vivo study the effect of Ca /Co-HAp nanoparticles (calcium/cobalt-hydroxiapatite) in the regeneration of the reduced alveolar bone density in experimental animals was examined by analyzing both the biochemical and histochemical markers and through analysis of alveolar bone density. The research was carried out on female Westar rats, aged 6-8 weeks, whom have been implanted nanobiomaterial in reduced alveolar bone density. Good results in regeneration of reduced bone density were achieved in six weeks after implantation of the nanoparticles with paramagnetic properties. Biochemical markers of osteogenesis showed statistically significant rise. Histological analysis revealed high level reparatory skills of the biocomposite implanted in the bone defect, while X-ray analyses evidenced rise of bone density. Implantation of Ca/Co-Hap enables rapid new formation, thus becoming the material of choice for accelerated reduced bone density regeneration.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts",
title = "Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density",
pages = "161-161",
url = "https://hdl.handle.net/21.15107/rcub_dais_674"
}
Ajduković, Z., Petrović, M. B., Milićević, J., Đorđević, N., Ignjatović, N., Savić, V., Najman, S., Mihailović, D., Vasiljević, P.,& Uskoković, D.. (2011). Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density. in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 161-161.
https://hdl.handle.net/21.15107/rcub_dais_674
Ajduković Z, Petrović MB, Milićević J, Đorđević N, Ignjatović N, Savić V, Najman S, Mihailović D, Vasiljević P, Uskoković D. Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density. in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts. 2011;:161-161.
https://hdl.handle.net/21.15107/rcub_dais_674 .
Ajduković, Zorica, Petrović, Milica B., Milićević, Jelena, Đorđević, Nadica, Ignjatović, Nenad, Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Vasiljević, Perica, Uskoković, Dragan, "Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density" in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts (2011):161-161,
https://hdl.handle.net/21.15107/rcub_dais_674 .

Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines

Najman, Stevo; Stojanović, Sanja; Najdanović, Jelena; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Institute of Technical Sciences of SASA, 2011)

TY  - CONF
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Najdanović, Jelena
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/698
AB  - The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
T1  - Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines
SP  - 6
EP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_dais_698
ER  - 
@conference{
author = "Najman, Stevo and Stojanović, Sanja and Najdanović, Jelena and Ignjatović, Nenad and Uskoković, Dragan",
year = "2011",
abstract = "The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia",
title = "Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines",
pages = "6-6",
url = "https://hdl.handle.net/21.15107/rcub_dais_698"
}
Najman, S., Stojanović, S., Najdanović, J., Ignjatović, N.,& Uskoković, D.. (2011). Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 6-6.
https://hdl.handle.net/21.15107/rcub_dais_698
Najman S, Stojanović S, Najdanović J, Ignjatović N, Uskoković D. Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:6-6.
https://hdl.handle.net/21.15107/rcub_dais_698 .
Najman, Stevo, Stojanović, Sanja, Najdanović, Jelena, Ignjatović, Nenad, Uskoković, Dragan, "Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines" in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):6-6,
https://hdl.handle.net/21.15107/rcub_dais_698 .