TY  - JOUR
AU  - Lazarević, Miloš M.
AU  - Ignjatović, Nenad
AU  - Mahlet, Qene
AU  - Bumah, Violet V.
AU  - Radunović, Milena
AU  - Milašin, Jelena
AU  - Uskoković, Dragan
AU  - Uskoković, Vuk
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16513
AB  - Hydroxyapatite (HAp) has been the main protagonist in the quest for an ideal biomaterial for regenerative medicine over the last half a century. To control its properties, this material has commonly been doped with chemical elements other than its natural stoichiometric constituents: Ca, O, P, and H. Here, we report on the first analysis of the biological response to germanium-doped hydroxyapatite (Ge-HAp). Cytotoxicity, osteogenic differentiation induction, and colony formation potential were measured on dental pulp stem cells, while the antimicrobial effect was assessed against Gram-negative Escherichia coli, Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), and Candida albicans. All analyses were run in comparison to Ge-free HAp. Cell viability was inversely dependent on the nanoparticle concentration and incubation time. Adding Ge to HAp reduced cell viability relative to HAp after 24–72 h incubation periods, but the effect was reversed after longer incubations, when the viability of cells treated with low doses of Ge-HAp exceeded that of HAp-treated cells and became comparable with control culture. Both HAp and Ge-HAp induced mineral formation in the cell culture, but the effect was more pronounced for Ge-HAp. Likewise, relative to both control cells and cells exposed to HAp, Ge-HAp upregulated the expression of all three osteogenic markers analyzed, namely, alkaline phosphatase, RUNX2, and osteocalcin, exerting the key influence on osteogenesis in its early, differentiation stage. The colony formation capacity of stem cells, however, was impaired by HAp and even more so by Ge-HAp. The antimicrobial effect was dependent on the microorganisms tested. Thus, whereas the antimicrobial activity was absent against E. coli, it was evident against MRSA and C. albicans. While the antibacterial activity against MRSA was weakened by the addition of Ge to HAp, the antimycotic activity against C. albicans was intensified with the addition of Ge. These findings demonstrate a significant potential of Ge-doped HAp nanoparticles in regenerative medicine due to their pronounced biocompatibility, osteoinductivity, and antimicrobial activity.
PB  - American Chemical Society (ACS)
T2  - ACS Applied Nano Materials
T1  - Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity
DO  - 10.1021/acsanm.3c05974
UR  - https://hdl.handle.net/21.15107/rcub_dais_16513
ER  - 

@article{
author = "Lazarević, Miloš M. and Ignjatović, Nenad and Mahlet, Qene and Bumah, Violet V. and Radunović, Milena and Milašin, Jelena and Uskoković, Dragan and Uskoković, Vuk",
year = "2024",
abstract = "Hydroxyapatite (HAp) has been the main protagonist in the quest for an ideal biomaterial for regenerative medicine over the last half a century. To control its properties, this material has commonly been doped with chemical elements other than its natural stoichiometric constituents: Ca, O, P, and H. Here, we report on the first analysis of the biological response to germanium-doped hydroxyapatite (Ge-HAp). Cytotoxicity, osteogenic differentiation induction, and colony formation potential were measured on dental pulp stem cells, while the antimicrobial effect was assessed against Gram-negative Escherichia coli, Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), and Candida albicans. All analyses were run in comparison to Ge-free HAp. Cell viability was inversely dependent on the nanoparticle concentration and incubation time. Adding Ge to HAp reduced cell viability relative to HAp after 24–72 h incubation periods, but the effect was reversed after longer incubations, when the viability of cells treated with low doses of Ge-HAp exceeded that of HAp-treated cells and became comparable with control culture. Both HAp and Ge-HAp induced mineral formation in the cell culture, but the effect was more pronounced for Ge-HAp. Likewise, relative to both control cells and cells exposed to HAp, Ge-HAp upregulated the expression of all three osteogenic markers analyzed, namely, alkaline phosphatase, RUNX2, and osteocalcin, exerting the key influence on osteogenesis in its early, differentiation stage. The colony formation capacity of stem cells, however, was impaired by HAp and even more so by Ge-HAp. The antimicrobial effect was dependent on the microorganisms tested. Thus, whereas the antimicrobial activity was absent against E. coli, it was evident against MRSA and C. albicans. While the antibacterial activity against MRSA was weakened by the addition of Ge to HAp, the antimycotic activity against C. albicans was intensified with the addition of Ge. These findings demonstrate a significant potential of Ge-doped HAp nanoparticles in regenerative medicine due to their pronounced biocompatibility, osteoinductivity, and antimicrobial activity.",
publisher = "American Chemical Society (ACS)",
journal = "ACS Applied Nano Materials",
title = "Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity",
doi = "10.1021/acsanm.3c05974",
url = "https://hdl.handle.net/21.15107/rcub_dais_16513"
}

Lazarević, M. M., Ignjatović, N., Mahlet, Q., Bumah, V. V., Radunović, M., Milašin, J., Uskoković, D.,& Uskoković, V.. (2024). Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity. in ACS Applied Nano Materials
American Chemical Society (ACS)..
https://doi.org/10.1021/acsanm.3c05974
https://hdl.handle.net/21.15107/rcub_dais_16513

Lazarević MM, Ignjatović N, Mahlet Q, Bumah VV, Radunović M, Milašin J, Uskoković D, Uskoković V. Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity. in ACS Applied Nano Materials. 2024;.
doi:10.1021/acsanm.3c05974
https://hdl.handle.net/21.15107/rcub_dais_16513 .

Lazarević, Miloš M., Ignjatović, Nenad, Mahlet, Qene, Bumah, Violet V., Radunović, Milena, Milašin, Jelena, Uskoković, Dragan, Uskoković, Vuk, "Biocompatible Germanium-Doped Hydroxyapatite Nanoparticles for Promoting Osteogenic Differentiation and Antimicrobial Activity" in ACS Applied Nano Materials (2024),
https://doi.org/10.1021/acsanm.3c05974 .,
https://hdl.handle.net/21.15107/rcub_dais_16513 .

TY  - JOUR
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Rabanal, Maria Eugenia
AU  - Milošević, Milica
AU  - Bukumira, Marta
AU  - Tomić, Nina
AU  - Tomić, Miloš
AU  - Mančić, Lidija
AU  - Ignjatović, Nenad
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16211
AB  - Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals
SP  - 6
VL  - 15
IS  - 1
DO  - 10.3390/jfb15010006
UR  - https://hdl.handle.net/21.15107/rcub_dais_16211
ER  - 

@article{
author = "Dinić, Ivana and Vuković, Marina and Rabanal, Maria Eugenia and Milošević, Milica and Bukumira, Marta and Tomić, Nina and Tomić, Miloš and Mančić, Lidija and Ignjatović, Nenad",
year = "2024",
abstract = "Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals",
pages = "6",
volume = "15",
number = "1",
doi = "10.3390/jfb15010006",
url = "https://hdl.handle.net/21.15107/rcub_dais_16211"
}

Dinić, I., Vuković, M., Rabanal, M. E., Milošević, M., Bukumira, M., Tomić, N., Tomić, M., Mančić, L.,& Ignjatović, N.. (2024). Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials
Basel : MDPI AG., 15(1), 6.
https://doi.org/10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211

Dinić I, Vuković M, Rabanal ME, Milošević M, Bukumira M, Tomić N, Tomić M, Mančić L, Ignjatović N. Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials. 2024;15(1):6.
doi:10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211 .

Dinić, Ivana, Vuković, Marina, Rabanal, Maria Eugenia, Milošević, Milica, Bukumira, Marta, Tomić, Nina, Tomić, Miloš, Mančić, Lidija, Ignjatović, Nenad, "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals" in Journal of Functional Biomaterials, 15, no. 1 (2024):6,
https://doi.org/10.3390/jfb15010006 .,
https://hdl.handle.net/21.15107/rcub_dais_16211 .

TY  - JOUR
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Živković, Jelena
AU  - Najdanović, Jelena
AU  - Radenković, Milena
AU  - Vasiljević, Perica
AU  - Ignjatović, Nenad
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14751
AB  - Regenerative medicine (RM) exploits the innate potential of the human body to effectively repair and regenerate damaged tissues and organs with the help of various biomaterials. Tissue engineering (TE) makes it possible to replace damaged tissues and organs with new ones. Research in the field of biomaterials has significantly improved the area of RM and TE. Biomaterials are used as orthopedic, dental, cardiovascular implants, medical devices, in the fields of reconstructive and regenerative medicine, among others. Important preconditions for the biomaterial to be used for implantation are its biocompatibility and biofunctionality. Biomaterials should enable adhesion, migration, proliferation and differentiation of cells. The biological properties of biomaterials are a reflection of their physicochemical properties, such as internal architecture, surface characteristics and charge. Biomaterials used in tissue regeneration should mimic the natural structure of the extracellular matrix and represent a physiological microenvironment for normal cellular functions. These biomaterials should also have adequate biodegradability properties to facilitate the formation and growth of new tissue. Biomaterials for use in RM can be of natural or synthetic origin, polymers by structural properties, ceramic and composite type, and based on bioreactivity they can be bioinert or bioactive. In RM and TE, polymers of different classes, natural and synthetic, are used, which can be made as intelligent materials. The structure of hydrogels in the form of a porous network represents a good matrix for cell activity. Ceramic biomaterials based on hydroxyapatite (HAp) are selected for use in RM and TE, especially solid tissues. Properties, such as composition, particle size, material shape, porosity, surface charge, topography, etc., are relevant for the proper use of HAp materials. The properties of HAp allow modification of its structure, surface, particle size design at the micro and nano level, hybridization with polymers, metals, etc. which is very important for its applications. Designed micro-nanohybrid HAp structure is most similar to the bone structure, making the cell environment closest to natural. Bone tissue engineering (BTE) is based on the combined use of cells, osteostimulating (osteoinductive) factors and biomaterials as a scaffolds and carriers for bone regeneration and defects repair. In BTE adipose-derived mesenchymal stem cells (ADSCs) are often used that are induced in vitro towards osteogenic cells or endothelial cells, and freshly isolated stromal vascular fraction can also be used. Blood components (PRP, blood plasma or blood clot) can be included in the composition of the BTE construct as a source of osteoinductive factors. In vitro models and methods were used to examine the biocompatibility, immunomodulatory and regenerative potential of biomaterials, as well as their influence on cellular functions. After in vitro methods, and before clinical studies, various in vivo animal models are used to examine the regenerative potential of biomaterials, such as subcutaneous implantations and bone defects in tibia, femur and calvaria in experimental animals (mouse, rat, rabbit).
PB  - Banja Luka : Academy of Sciences and Arts of the Republic of Srpska
T2  - Contemporary Materials
T1  - Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective
VL  - 14
IS  - 1
DO  - 10.7251/COMEN2301001N
UR  - https://hdl.handle.net/21.15107/rcub_dais_14751
ER  - 

@article{
author = "Najman, Stevo and Stojanović, Sanja and Živković, Jelena and Najdanović, Jelena and Radenković, Milena and Vasiljević, Perica and Ignjatović, Nenad",
year = "2023",
abstract = "Regenerative medicine (RM) exploits the innate potential of the human body to effectively repair and regenerate damaged tissues and organs with the help of various biomaterials. Tissue engineering (TE) makes it possible to replace damaged tissues and organs with new ones. Research in the field of biomaterials has significantly improved the area of RM and TE. Biomaterials are used as orthopedic, dental, cardiovascular implants, medical devices, in the fields of reconstructive and regenerative medicine, among others. Important preconditions for the biomaterial to be used for implantation are its biocompatibility and biofunctionality. Biomaterials should enable adhesion, migration, proliferation and differentiation of cells. The biological properties of biomaterials are a reflection of their physicochemical properties, such as internal architecture, surface characteristics and charge. Biomaterials used in tissue regeneration should mimic the natural structure of the extracellular matrix and represent a physiological microenvironment for normal cellular functions. These biomaterials should also have adequate biodegradability properties to facilitate the formation and growth of new tissue. Biomaterials for use in RM can be of natural or synthetic origin, polymers by structural properties, ceramic and composite type, and based on bioreactivity they can be bioinert or bioactive. In RM and TE, polymers of different classes, natural and synthetic, are used, which can be made as intelligent materials. The structure of hydrogels in the form of a porous network represents a good matrix for cell activity. Ceramic biomaterials based on hydroxyapatite (HAp) are selected for use in RM and TE, especially solid tissues. Properties, such as composition, particle size, material shape, porosity, surface charge, topography, etc., are relevant for the proper use of HAp materials. The properties of HAp allow modification of its structure, surface, particle size design at the micro and nano level, hybridization with polymers, metals, etc. which is very important for its applications. Designed micro-nanohybrid HAp structure is most similar to the bone structure, making the cell environment closest to natural. Bone tissue engineering (BTE) is based on the combined use of cells, osteostimulating (osteoinductive) factors and biomaterials as a scaffolds and carriers for bone regeneration and defects repair. In BTE adipose-derived mesenchymal stem cells (ADSCs) are often used that are induced in vitro towards osteogenic cells or endothelial cells, and freshly isolated stromal vascular fraction can also be used. Blood components (PRP, blood plasma or blood clot) can be included in the composition of the BTE construct as a source of osteoinductive factors. In vitro models and methods were used to examine the biocompatibility, immunomodulatory and regenerative potential of biomaterials, as well as their influence on cellular functions. After in vitro methods, and before clinical studies, various in vivo animal models are used to examine the regenerative potential of biomaterials, such as subcutaneous implantations and bone defects in tibia, femur and calvaria in experimental animals (mouse, rat, rabbit).",
publisher = "Banja Luka : Academy of Sciences and Arts of the Republic of Srpska",
journal = "Contemporary Materials",
title = "Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective",
volume = "14",
number = "1",
doi = "10.7251/COMEN2301001N",
url = "https://hdl.handle.net/21.15107/rcub_dais_14751"
}

Najman, S., Stojanović, S., Živković, J., Najdanović, J., Radenković, M., Vasiljević, P.,& Ignjatović, N.. (2023). Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective. in Contemporary Materials
Banja Luka : Academy of Sciences and Arts of the Republic of Srpska., 14(1).
https://doi.org/10.7251/COMEN2301001N
https://hdl.handle.net/21.15107/rcub_dais_14751

Najman S, Stojanović S, Živković J, Najdanović J, Radenković M, Vasiljević P, Ignjatović N. Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective. in Contemporary Materials. 2023;14(1).
doi:10.7251/COMEN2301001N
https://hdl.handle.net/21.15107/rcub_dais_14751 .

Najman, Stevo, Stojanović, Sanja, Živković, Jelena, Najdanović, Jelena, Radenković, Milena, Vasiljević, Perica, Ignjatović, Nenad, "Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective" in Contemporary Materials, 14, no. 1 (2023),
https://doi.org/10.7251/COMEN2301001N .,
https://hdl.handle.net/21.15107/rcub_dais_14751 .

TY  - CONF
AU  - Lazarević, Miloš M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Uskoković, Vuk
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15765
AB  - Most of the applications for various forms of ion-doped hydroxyapatite have been geared towards pharmacy, dentistry and medicine. This study presents the first biological, response to germanium-doped hydroxyapatite (Ge-HAp). Viability, osteogenic differentiation induction and colony formation potential of dental pulp stem cells (DPSCs) in the presence of Ge-HAp and pure, Ge-free HAp nanoparticles was assessed. DPSCs were isolated from semiimpacted wisdom teeth extracted from systemically sound patients. MTT was used to determine cell viability after 1, 3, and 7 days of incubation. The effect of Ge-HAp on the expression level of osteodifferentiation markers (RUNX2, ALP, and OCN) was determined using RT-qPCR, and mineralized nodule formation was confirmed using Alizarin Red S staining. The colony-forming unit assay was utilized to evaluate the colony-formation potential of the DPSC. Low dosages of Ge-HAp increased cell viability compared to HAp after a week. Ge-HAp increased cell culture mineralization more than HAp. Ge-HAp substantially upregulated all three osteogenic markers relative to control and Ge-free HApexposed cells. HAp and especially Ge-HAp hindered stem cell colony formation. As demonstrated above, Ge-doped HAp nanoparticles have great promise in regenerative medicine due to their biocompatibility and osteoinductivity.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
T1  - The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_dais_15765
ER  - 

@conference{
author = "Lazarević, Miloš M. and Ignjatović, Nenad and Uskoković, Dragan and Uskoković, Vuk",
year = "2023",
abstract = "Most of the applications for various forms of ion-doped hydroxyapatite have been geared towards pharmacy, dentistry and medicine. This study presents the first biological, response to germanium-doped hydroxyapatite (Ge-HAp). Viability, osteogenic differentiation induction and colony formation potential of dental pulp stem cells (DPSCs) in the presence of Ge-HAp and pure, Ge-free HAp nanoparticles was assessed. DPSCs were isolated from semiimpacted wisdom teeth extracted from systemically sound patients. MTT was used to determine cell viability after 1, 3, and 7 days of incubation. The effect of Ge-HAp on the expression level of osteodifferentiation markers (RUNX2, ALP, and OCN) was determined using RT-qPCR, and mineralized nodule formation was confirmed using Alizarin Red S staining. The colony-forming unit assay was utilized to evaluate the colony-formation potential of the DPSC. Low dosages of Ge-HAp increased cell viability compared to HAp after a week. Ge-HAp increased cell culture mineralization more than HAp. Ge-HAp substantially upregulated all three osteogenic markers relative to control and Ge-free HApexposed cells. HAp and especially Ge-HAp hindered stem cell colony formation. As demonstrated above, Ge-doped HAp nanoparticles have great promise in regenerative medicine due to their biocompatibility and osteoinductivity.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023",
title = "The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_dais_15765"
}

Lazarević, M. M., Ignjatović, N., Uskoković, D.,& Uskoković, V.. (2023). The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
Belgrade : Serbian Ceramic Society., 67-67.
https://hdl.handle.net/21.15107/rcub_dais_15765

Lazarević MM, Ignjatović N, Uskoković D, Uskoković V. The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023. 2023;:67-67.
https://hdl.handle.net/21.15107/rcub_dais_15765 .

Lazarević, Miloš M., Ignjatović, Nenad, Uskoković, Dragan, Uskoković, Vuk, "The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 (2023):67-67,
https://hdl.handle.net/21.15107/rcub_dais_15765 .

TY  - JOUR
AU  - Lazarević, Miloš
AU  - Petrović, Sanja
AU  - Pierfelice, Tania Vanessa
AU  - Ignjatović, Nenad
AU  - Piattelli, Adriano
AU  - Vlajić Tovilović, Tamara
AU  - Radunović, Milena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14287
AB  - Collagen membranes are routinely used in oral surgery for bone regeneration. Despite their numerous advantages, such as stimulating bone growth, bacterial contamination still remains one of the disadvantages of membrane use. Thus, we assessed the biocompatibility and osteogenic and antibacterial properties of a collagen membrane (OsteoBiol) modified with chitosan (CHI) and hydroxyapatite nanoparticles (HApNPs). Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FT-IR), X-ray powder diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) were performed for membrane characterization. Biocompatibility was assessed on dental pulp stem cells (DPSCs) by an MTT assay, while the osteogenic effect was assessed by an ALP activity assay and qPCR analysis of osteogenic markers (BMP4, ALP, RUNX2, and OCN). Antimicrobial properties were investigated by counting colony-forming units (CFUs) of Streptococcus mitis, Porphyromonas gingivalis, and Fusobaterium nucleatum on membranes and in the surrounding medium. Membranes showed no cytotoxicity. ALP activity was higher and ALP, BMP4, and OCN genes were up-regulated in DPSCs on modified membranes compared to unmodified membranes. The CFUs were reduced on modified membranes and in the medium. Modified membranes showed great biocompatibility and a high osteoinductive effect. Additionally, they showed antimicrobial and antibiofilm effects against periopathogens. It can be concluded that the incorporation of CHI and hydroxyapatite nanoparticles in collagen membranes may be advantageous to promote osteogenesis and reduce bacterial adhesion.
PB  - Basel : MDPI AG
T2  - Biomolecules
T1  - Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite
SP  - 579
VL  - 13
IS  - 4
DO  - 10.3390/biom13040579
UR  - https://hdl.handle.net/21.15107/rcub_dais_14287
ER  - 

@article{
author = "Lazarević, Miloš and Petrović, Sanja and Pierfelice, Tania Vanessa and Ignjatović, Nenad and Piattelli, Adriano and Vlajić Tovilović, Tamara and Radunović, Milena",
year = "2023",
abstract = "Collagen membranes are routinely used in oral surgery for bone regeneration. Despite their numerous advantages, such as stimulating bone growth, bacterial contamination still remains one of the disadvantages of membrane use. Thus, we assessed the biocompatibility and osteogenic and antibacterial properties of a collagen membrane (OsteoBiol) modified with chitosan (CHI) and hydroxyapatite nanoparticles (HApNPs). Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FT-IR), X-ray powder diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) were performed for membrane characterization. Biocompatibility was assessed on dental pulp stem cells (DPSCs) by an MTT assay, while the osteogenic effect was assessed by an ALP activity assay and qPCR analysis of osteogenic markers (BMP4, ALP, RUNX2, and OCN). Antimicrobial properties were investigated by counting colony-forming units (CFUs) of Streptococcus mitis, Porphyromonas gingivalis, and Fusobaterium nucleatum on membranes and in the surrounding medium. Membranes showed no cytotoxicity. ALP activity was higher and ALP, BMP4, and OCN genes were up-regulated in DPSCs on modified membranes compared to unmodified membranes. The CFUs were reduced on modified membranes and in the medium. Modified membranes showed great biocompatibility and a high osteoinductive effect. Additionally, they showed antimicrobial and antibiofilm effects against periopathogens. It can be concluded that the incorporation of CHI and hydroxyapatite nanoparticles in collagen membranes may be advantageous to promote osteogenesis and reduce bacterial adhesion.",
publisher = "Basel : MDPI AG",
journal = "Biomolecules",
title = "Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite",
pages = "579",
volume = "13",
number = "4",
doi = "10.3390/biom13040579",
url = "https://hdl.handle.net/21.15107/rcub_dais_14287"
}

Lazarević, M., Petrović, S., Pierfelice, T. V., Ignjatović, N., Piattelli, A., Vlajić Tovilović, T.,& Radunović, M.. (2023). Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite. in Biomolecules
Basel : MDPI AG., 13(4), 579.
https://doi.org/10.3390/biom13040579
https://hdl.handle.net/21.15107/rcub_dais_14287

Lazarević M, Petrović S, Pierfelice TV, Ignjatović N, Piattelli A, Vlajić Tovilović T, Radunović M. Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite. in Biomolecules. 2023;13(4):579.
doi:10.3390/biom13040579
https://hdl.handle.net/21.15107/rcub_dais_14287 .

Lazarević, Miloš, Petrović, Sanja, Pierfelice, Tania Vanessa, Ignjatović, Nenad, Piattelli, Adriano, Vlajić Tovilović, Tamara, Radunović, Milena, "Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite" in Biomolecules, 13, no. 4 (2023):579,
https://doi.org/10.3390/biom13040579 .,
https://hdl.handle.net/21.15107/rcub_dais_14287 .

TY  - JOUR
AU  - Canullo, Luigi
AU  - Rakic, Mia
AU  - Corvino, Emilio
AU  - Burton, Maria
AU  - Krumbeck, Janina A.
AU  - Chittoor Prem, Aishani
AU  - Ravidà, Andrea
AU  - Ignjatović, Nenad
AU  - Sculean, Anton
AU  - Menini, Maria
AU  - Pesce, Paolo
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/13750
AB  - Purpose: Biofilm-free implant surface is ultimate prerequisite for successful soft and bone tissue integration. Objective of the study was to estimate the effects of argon plasma healing abutment pre-treatment (PT) on peri-implant soft-tissue phenotype (PiSP), inflammation, plaque accumulation and the microbiome (PiM) between non-treated (NPT) and treated (PT) abutments following 3-months healing period. The hypothesis was that cell-conductive and antimicrobial properties of PT would yield optimal conditions for soft tissue integration.
Material and Methods: Two months following second-phase surgery, microbiological and clinical parameters were assessed around thirty-six healing abutments with two types of microtopography, smooth surface (MACHINED) and ultrathin threaded microsurface (ROUGH). A two level randomization schema was used to achieve equal distribution and abutments were randomly divided into rough and machined groups, and then divided into PT and NPT groups. PiM was assessed using next-generation DNA sequencing.
Results: PiM bacterial composition was highly diverse already two months post-implantation, consisting of key-stone pathogens, early and late colonizers, while the mycobiome was less diverse. PT was associated with lower plaque accumulation and inflammation without significant impact on PiSP, while in NPT clinical parameters were increased and associated with periopathogens. NPT mostly harbored late colonizers, while PT exerted higher abundance of early colonizers suggesting less advanced plaque formation. Interaction analysis in PT demonstrated S. mitis co-occurrence with pro-healthy Rothia dentocariosa and co-exclusion with Parvimonas micra, Porphyromonas endodontalis and Prevotella oris. PiSP parameters were generally similar between the groups, but significant association between PiM and keratinized mucosa width was observed in both groups, with remarkably more expressed diversity in NPT compared to PT. PT resulted in significantly lower BOP and PI around rough and machined abutments, respectively, without specific effect on PiM and PiSP.
Conclusions: PT contributed to significantly the less advanced biofilm accumulation and inflammation without specific effects on PiSP.
PB  - Springer Science and Business Media LLC
T2  - BMC Oral Health
T1  - Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study
VL  - 23
IS  - 1
DO  - 10.1186/s12903-023-02729-1
UR  - https://hdl.handle.net/21.15107/rcub_dais_13750
ER  - 

@article{
author = "Canullo, Luigi and Rakic, Mia and Corvino, Emilio and Burton, Maria and Krumbeck, Janina A. and Chittoor Prem, Aishani and Ravidà, Andrea and Ignjatović, Nenad and Sculean, Anton and Menini, Maria and Pesce, Paolo",
year = "2023",
abstract = "Purpose: Biofilm-free implant surface is ultimate prerequisite for successful soft and bone tissue integration. Objective of the study was to estimate the effects of argon plasma healing abutment pre-treatment (PT) on peri-implant soft-tissue phenotype (PiSP), inflammation, plaque accumulation and the microbiome (PiM) between non-treated (NPT) and treated (PT) abutments following 3-months healing period. The hypothesis was that cell-conductive and antimicrobial properties of PT would yield optimal conditions for soft tissue integration.
Material and Methods: Two months following second-phase surgery, microbiological and clinical parameters were assessed around thirty-six healing abutments with two types of microtopography, smooth surface (MACHINED) and ultrathin threaded microsurface (ROUGH). A two level randomization schema was used to achieve equal distribution and abutments were randomly divided into rough and machined groups, and then divided into PT and NPT groups. PiM was assessed using next-generation DNA sequencing.
Results: PiM bacterial composition was highly diverse already two months post-implantation, consisting of key-stone pathogens, early and late colonizers, while the mycobiome was less diverse. PT was associated with lower plaque accumulation and inflammation without significant impact on PiSP, while in NPT clinical parameters were increased and associated with periopathogens. NPT mostly harbored late colonizers, while PT exerted higher abundance of early colonizers suggesting less advanced plaque formation. Interaction analysis in PT demonstrated S. mitis co-occurrence with pro-healthy Rothia dentocariosa and co-exclusion with Parvimonas micra, Porphyromonas endodontalis and Prevotella oris. PiSP parameters were generally similar between the groups, but significant association between PiM and keratinized mucosa width was observed in both groups, with remarkably more expressed diversity in NPT compared to PT. PT resulted in significantly lower BOP and PI around rough and machined abutments, respectively, without specific effect on PiM and PiSP.
Conclusions: PT contributed to significantly the less advanced biofilm accumulation and inflammation without specific effects on PiSP.",
publisher = "Springer Science and Business Media LLC",
journal = "BMC Oral Health",
title = "Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study",
volume = "23",
number = "1",
doi = "10.1186/s12903-023-02729-1",
url = "https://hdl.handle.net/21.15107/rcub_dais_13750"
}

Canullo, L., Rakic, M., Corvino, E., Burton, M., Krumbeck, J. A., Chittoor Prem, A., Ravidà, A., Ignjatović, N., Sculean, A., Menini, M.,& Pesce, P.. (2023). Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study. in BMC Oral Health
Springer Science and Business Media LLC., 23(1).
https://doi.org/10.1186/s12903-023-02729-1
https://hdl.handle.net/21.15107/rcub_dais_13750

Canullo L, Rakic M, Corvino E, Burton M, Krumbeck JA, Chittoor Prem A, Ravidà A, Ignjatović N, Sculean A, Menini M, Pesce P. Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study. in BMC Oral Health. 2023;23(1).
doi:10.1186/s12903-023-02729-1
https://hdl.handle.net/21.15107/rcub_dais_13750 .

Canullo, Luigi, Rakic, Mia, Corvino, Emilio, Burton, Maria, Krumbeck, Janina A., Chittoor Prem, Aishani, Ravidà, Andrea, Ignjatović, Nenad, Sculean, Anton, Menini, Maria, Pesce, Paolo, "Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study" in BMC Oral Health, 23, no. 1 (2023),
https://doi.org/10.1186/s12903-023-02729-1 .,
https://hdl.handle.net/21.15107/rcub_dais_13750 .

TY  - JOUR
AU  - Dorm, Bruna Carolina
AU  - Iemma, Mônica Rosas Costa
AU  - Neto, Benedito Domingos
AU  - Francisco, Rauany Cristina Lopes
AU  - Dinić, Ivana
AU  - Ignjatović, Nenad
AU  - Marković, Smilja
AU  - Vuković, Marina
AU  - Škapin, Srečo
AU  - Trovatti, Eliane
AU  - Mančić, Lidija
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/13685
AB  - Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.
T2  - Life
T1  - Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles
SP  - 116
VL  - 13
IS  - 1
DO  - 10.3390/life13010116
UR  - https://hdl.handle.net/21.15107/rcub_dais_13685
ER  - 

@article{
author = "Dorm, Bruna Carolina and Iemma, Mônica Rosas Costa and Neto, Benedito Domingos and Francisco, Rauany Cristina Lopes and Dinić, Ivana and Ignjatović, Nenad and Marković, Smilja and Vuković, Marina and Škapin, Srečo and Trovatti, Eliane and Mančić, Lidija",
year = "2023",
abstract = "Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.",
journal = "Life",
title = "Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles",
pages = "116",
volume = "13",
number = "1",
doi = "10.3390/life13010116",
url = "https://hdl.handle.net/21.15107/rcub_dais_13685"
}

Dorm, B. C., Iemma, M. R. C., Neto, B. D., Francisco, R. C. L., Dinić, I., Ignjatović, N., Marković, S., Vuković, M., Škapin, S., Trovatti, E.,& Mančić, L.. (2023). Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles. in Life, 13(1), 116.
https://doi.org/10.3390/life13010116
https://hdl.handle.net/21.15107/rcub_dais_13685

Dorm BC, Iemma MRC, Neto BD, Francisco RCL, Dinić I, Ignjatović N, Marković S, Vuković M, Škapin S, Trovatti E, Mančić L. Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles. in Life. 2023;13(1):116.
doi:10.3390/life13010116
https://hdl.handle.net/21.15107/rcub_dais_13685 .

Dorm, Bruna Carolina, Iemma, Mônica Rosas Costa, Neto, Benedito Domingos, Francisco, Rauany Cristina Lopes, Dinić, Ivana, Ignjatović, Nenad, Marković, Smilja, Vuković, Marina, Škapin, Srečo, Trovatti, Eliane, Mančić, Lidija, "Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles" in Life, 13, no. 1 (2023):116,
https://doi.org/10.3390/life13010116 .,
https://hdl.handle.net/21.15107/rcub_dais_13685 .

TY  - JOUR
AU  - Lović, Jelena
AU  - Eraković Pantović, Sanja
AU  - Rakočević, Lazar
AU  - Ignjatović, Nenad
AU  - Dimitrijević, Silvana B.
AU  - Nikolić, Nebojša D.
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/13684
AB  - Sn-Pd electrocatalysts with a constant atomic ratio of 60 at.% Sn‒40 at.% Pd suitable for potential application in direct ethanol fuel cells were synthesized using a novel two-step electrodeposition method. First, Sn was electrodeposited in various forms of dendrites, from spear-like and needle-like to individual fern-like dendrites to a network of intertwined fern-like dendrites, by varying the cathodic potential and then performing electrodeposition of Pd at a constant current density in the second step. A morphological and elemental analysis of Sn and Sn-Pd electrocatalysts was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) techniques, while the size of Sn dendrites was analyzed using the particle size distribution (PSD) method. Cyclic voltammetry (CV) and chronoamperometry were applied in order to study the catalytic behavior of Sn-Pd electrocatalysts in the ethanol oxidation reaction (EOR), while CO stripping was used to estimate the antipoisoning capability of the electrocatalysts. The Sn surface morphology of the sub-layer was highly correlated with the electrocatalytic activity of the examined Sn-Pd electrocatalysts. The high activity it presented towards the EOR showed the suitability of the Sn-Pd electrocatalyst constructed from individual fern-like Sn dendrites as a sub-layer. Compared to Pd alone, this Sn-Pd catalyst showed more than 3 times higher activity and improved EOR kinetics. This enhancement in the catalytic activity of the Sn-Pd electrocatalysts is attributed to both the morphological characteristics of Sn as a sub-layer and the bifunctional effect.
T2  - Processes
T1  - A Novel Two-Step Electrochemical Deposition Method for Sn-Pd Electrocatalyst Synthesis for a Potential Application in Direct Ethanol Fuel Cells
SP  - 120
VL  - 11
IS  - 1
DO  - 10.3390/pr11010120
UR  - https://hdl.handle.net/21.15107/rcub_dais_13684
ER  - 

@article{
author = "Lović, Jelena and Eraković Pantović, Sanja and Rakočević, Lazar and Ignjatović, Nenad and Dimitrijević, Silvana B. and Nikolić, Nebojša D.",
year = "2023",
abstract = "Sn-Pd electrocatalysts with a constant atomic ratio of 60 at.% Sn‒40 at.% Pd suitable for potential application in direct ethanol fuel cells were synthesized using a novel two-step electrodeposition method. First, Sn was electrodeposited in various forms of dendrites, from spear-like and needle-like to individual fern-like dendrites to a network of intertwined fern-like dendrites, by varying the cathodic potential and then performing electrodeposition of Pd at a constant current density in the second step. A morphological and elemental analysis of Sn and Sn-Pd electrocatalysts was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) techniques, while the size of Sn dendrites was analyzed using the particle size distribution (PSD) method. Cyclic voltammetry (CV) and chronoamperometry were applied in order to study the catalytic behavior of Sn-Pd electrocatalysts in the ethanol oxidation reaction (EOR), while CO stripping was used to estimate the antipoisoning capability of the electrocatalysts. The Sn surface morphology of the sub-layer was highly correlated with the electrocatalytic activity of the examined Sn-Pd electrocatalysts. The high activity it presented towards the EOR showed the suitability of the Sn-Pd electrocatalyst constructed from individual fern-like Sn dendrites as a sub-layer. Compared to Pd alone, this Sn-Pd catalyst showed more than 3 times higher activity and improved EOR kinetics. This enhancement in the catalytic activity of the Sn-Pd electrocatalysts is attributed to both the morphological characteristics of Sn as a sub-layer and the bifunctional effect.",
journal = "Processes",
title = "A Novel Two-Step Electrochemical Deposition Method for Sn-Pd Electrocatalyst Synthesis for a Potential Application in Direct Ethanol Fuel Cells",
pages = "120",
volume = "11",
number = "1",
doi = "10.3390/pr11010120",
url = "https://hdl.handle.net/21.15107/rcub_dais_13684"
}

Lović, J., Eraković Pantović, S., Rakočević, L., Ignjatović, N., Dimitrijević, S. B.,& Nikolić, N. D.. (2023). A Novel Two-Step Electrochemical Deposition Method for Sn-Pd Electrocatalyst Synthesis for a Potential Application in Direct Ethanol Fuel Cells. in Processes, 11(1), 120.
https://doi.org/10.3390/pr11010120
https://hdl.handle.net/21.15107/rcub_dais_13684

Lović J, Eraković Pantović S, Rakočević L, Ignjatović N, Dimitrijević SB, Nikolić ND. A Novel Two-Step Electrochemical Deposition Method for Sn-Pd Electrocatalyst Synthesis for a Potential Application in Direct Ethanol Fuel Cells. in Processes. 2023;11(1):120.
doi:10.3390/pr11010120
https://hdl.handle.net/21.15107/rcub_dais_13684 .

Lović, Jelena, Eraković Pantović, Sanja, Rakočević, Lazar, Ignjatović, Nenad, Dimitrijević, Silvana B., Nikolić, Nebojša D., "A Novel Two-Step Electrochemical Deposition Method for Sn-Pd Electrocatalyst Synthesis for a Potential Application in Direct Ethanol Fuel Cells" in Processes, 11, no. 1 (2023):120,
https://doi.org/10.3390/pr11010120 .,
https://hdl.handle.net/21.15107/rcub_dais_13684 .

TY  - CHAP
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/13683
AB  - Mammal bone tissue is mainly composed of an inorganic component consisting of calcium phosphate (CaPCalcium phosphateCaP) ceramics, predominantly calcium hydroxyapatiteCalcium phosphatecalcium hydroxyapatite (HAp)Calcium phosphateHAp, in addition to the organic component. Properties of HAp enable the modification of its structure, surface, design of particle size at the micro and the macro levels, hybridization with polymers, metals, etc., which makes it a very important biomaterial in the future as well. The properties of HAp can be improved by combination with a large number of therapeuticBiomaterialtherapeuticCalcium phosphateBioceramics and/or diagnosticBiomaterialdiagnosticBioceramicsCalcium phosphate agents. CaP and HAp occupy a significant place in the research of different types of multifunctionalMaterialsmultifunctional and hybridMaterialshybrid materials for potential application in diagnostic, preventiveMaterialspreventive, oncological, reconstructiveMaterialsreconstructiveRegenerative medicine and regenerative medicine. There are numerous data on the properties of HAp and HAp-based biomaterials relevant to their use, such as composition, particle sizeBiomaterialparticle sizeBioceramicsCalcium phosphate, material shape, porosity, surface charge, topography, etc. This chapter also discusses the different properties of hydroxyapatite and hydroxyapatite-based biomaterials important for interactionBiomaterialinteractionTissueCell with cells and tissues. In addition to references to literature data, the results of our research with different hydroxyapatite-based biomaterials in different in vitroModelsin vitroCellMethod and in vivoModelsin vivoMethodTissue engineering experimentalModelsexperimental modelsModels in the field of bone tissue engineeringTissue engineeringbone tissue engineering and regenerative medicine are presented.
PB  - Springer International Publishing
T2  - Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications
T1  - Properties of Hydroxyapatite-Based Biomaterials Important for Interactions with Cells and Tissues
SP  - 115
EP  - 135
DO  - 10.1007/978-3-031-17269-4_6
UR  - https://hdl.handle.net/21.15107/rcub_dais_13683
ER  - 

@inbook{
author = "Živković, Jelena M. and Ignjatović, Nenad and Najman, Stevo",
year = "2023",
abstract = "Mammal bone tissue is mainly composed of an inorganic component consisting of calcium phosphate (CaPCalcium phosphateCaP) ceramics, predominantly calcium hydroxyapatiteCalcium phosphatecalcium hydroxyapatite (HAp)Calcium phosphateHAp, in addition to the organic component. Properties of HAp enable the modification of its structure, surface, design of particle size at the micro and the macro levels, hybridization with polymers, metals, etc., which makes it a very important biomaterial in the future as well. The properties of HAp can be improved by combination with a large number of therapeuticBiomaterialtherapeuticCalcium phosphateBioceramics and/or diagnosticBiomaterialdiagnosticBioceramicsCalcium phosphate agents. CaP and HAp occupy a significant place in the research of different types of multifunctionalMaterialsmultifunctional and hybridMaterialshybrid materials for potential application in diagnostic, preventiveMaterialspreventive, oncological, reconstructiveMaterialsreconstructiveRegenerative medicine and regenerative medicine. There are numerous data on the properties of HAp and HAp-based biomaterials relevant to their use, such as composition, particle sizeBiomaterialparticle sizeBioceramicsCalcium phosphate, material shape, porosity, surface charge, topography, etc. This chapter also discusses the different properties of hydroxyapatite and hydroxyapatite-based biomaterials important for interactionBiomaterialinteractionTissueCell with cells and tissues. In addition to references to literature data, the results of our research with different hydroxyapatite-based biomaterials in different in vitroModelsin vitroCellMethod and in vivoModelsin vivoMethodTissue engineering experimentalModelsexperimental modelsModels in the field of bone tissue engineeringTissue engineeringbone tissue engineering and regenerative medicine are presented.",
publisher = "Springer International Publishing",
journal = "Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications",
booktitle = "Properties of Hydroxyapatite-Based Biomaterials Important for Interactions with Cells and Tissues",
pages = "115-135",
doi = "10.1007/978-3-031-17269-4_6",
url = "https://hdl.handle.net/21.15107/rcub_dais_13683"
}

Živković, J. M., Ignjatović, N.,& Najman, S.. (2023). Properties of Hydroxyapatite-Based Biomaterials Important for Interactions with Cells and Tissues. in Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications
Springer International Publishing., 115-135.
https://doi.org/10.1007/978-3-031-17269-4_6
https://hdl.handle.net/21.15107/rcub_dais_13683

Živković JM, Ignjatović N, Najman S. Properties of Hydroxyapatite-Based Biomaterials Important for Interactions with Cells and Tissues. in Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications. 2023;:115-135.
doi:10.1007/978-3-031-17269-4_6
https://hdl.handle.net/21.15107/rcub_dais_13683 .

Živković, Jelena M., Ignjatović, Nenad, Najman, Stevo, "Properties of Hydroxyapatite-Based Biomaterials Important for Interactions with Cells and Tissues" in Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications (2023):115-135,
https://doi.org/10.1007/978-3-031-17269-4_6 .,
https://hdl.handle.net/21.15107/rcub_dais_13683 .

TY  - CONF
AU  - Nikolić, Nebojša D.
AU  - Lović, Jelena
AU  - Ignjatović, Nenad
AU  - Dimitrijević, Silvana B.
PY  - 2023
UR  - https://annual74.ise-online.org
UR  - https://dais.sanu.ac.rs/123456789/15643
AB  - Sn-Pd electrocatalysts convenient for a possible application in direct ethanol fuel cell were produced by two electrochemical deposition method. In the first step, Sn dendrites of various degree of ramification were electrodeposited by potentiostatically on cathodic potentials of –1200, –1400, –1600 and –1800 mV vs. Ag/AgCl. Electrodeposition of tin was performed from 20 g/l SnCl2 × 2H2O in 250 g/l NaOH electrolyte [1] at the room temperature on Cu electrodes. In the second step, Pd was electrodeposited galvanostatically at a current density of –5 mA cm-2 on the electrodes with electrodeposited Sn dendrites. Electrodeposition of Pd was performed from 1 M NH4Cl and 0.01 M PdCl2 at the room temperature [2]. The processes of electrochemical deposition of Sn and Pd were performed with amounts of the electricity of 400 and 267 mC, giving an atomic ratio synthesized the electrocatalysts of 60 at.% Sn‒40 at.% Pd. Morpological and elemental analysis of the Sn-Pd electrocatalysts was performed by scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS), respectively. The particle size distribution (PSD) was used to estimate a size of Sn dendrites. The electrocatalytic activity of synthesized Sn-Pd electrocatalysts towards ethanol oxidation reaction (EOR) was examined by cyclic voltammetry (CV) and chronoamperometry, using 1 M NaOH with 1 M ethanol solution. Depending on the applied cathodic potentials, the following shapes of dendrites were formed: the needle-like and the spear-like at –1200 mV, an individual fern-like dendrites at –1400 mV, and intertwined network of dendrites of the fern-like shape was formed at cathodic potentials of – 1600 and – 1800 mV vs. Ag/AgCl. The partial coverage of the Sn dendrites through a formation of compact islands of Pd was attained by electrodeposition of Pd on the electrodes with the Sn dendrites. It was shown that morphology of the Sn dendrites as sub-layer strongly correlates the electrocatalytic activity of the Sn-Pd electrocatalysts. The largest electrocatalytic activity showed the Sn- Pd electrocatalyst constructed from the individual fern-like Sn dendrites as sub-layer produced at a cathodic potential of –1400 mV vs. Ag/AgCl. This dendrite type showed more branchy morphology than those obtained at the other cathodic potentials. Formation of individual needle-like and spear-like dendrites at a cathodic potential of – 1200 mV vs. Ag/AgCl did not contribute significantly to the increase of the initial electrode surface area. On the other hand, due to a high nucleation rate, growth a dendrites from one nucleation centre in more directions, as well as the fact that Sn is very soft metal, morphological characteristics of intertwined Sn dendrites obtained at cathodic potentials of −1600 and −1800 mV vs. Ag/AgCl approached to those of compact massive Sn. The partial coverage of Sn dendrites by Pd can be attributed to the current density distribution effect, because Pd electrodeposition process preferentially perfomed at the higher parts of the surface area, i.e. on the dendrites, rather than on the flat part of the electrode surface area. Aside from an influence of degree of branchy of Sn dendrites as sub-layer on the electrocatalytic activity in ethanol oxidation, varios electrocatalytic performance of the Sn-Pd electrocatalysts can also be ascribed to the bifunctional effect.
PB  - Lausanne : International Society of Electrochemistry
C3  - The 74th Annual Meeting of the International Society of Electrochemistry : Bridging Scientific Disciplines to Address the World’s Challenges 3 to 8 September 2023. Lyon, France
T1  - Influence of Morphology of Sn Dendrites as Sub-layer on Electrolcatalytic Performance of Sn-Pd Electrocatalysts
UR  - https://hdl.handle.net/21.15107/rcub_dais_15643
ER  - 

@conference{
author = "Nikolić, Nebojša D. and Lović, Jelena and Ignjatović, Nenad and Dimitrijević, Silvana B.",
year = "2023",
abstract = "Sn-Pd electrocatalysts convenient for a possible application in direct ethanol fuel cell were produced by two electrochemical deposition method. In the first step, Sn dendrites of various degree of ramification were electrodeposited by potentiostatically on cathodic potentials of –1200, –1400, –1600 and –1800 mV vs. Ag/AgCl. Electrodeposition of tin was performed from 20 g/l SnCl2 × 2H2O in 250 g/l NaOH electrolyte [1] at the room temperature on Cu electrodes. In the second step, Pd was electrodeposited galvanostatically at a current density of –5 mA cm-2 on the electrodes with electrodeposited Sn dendrites. Electrodeposition of Pd was performed from 1 M NH4Cl and 0.01 M PdCl2 at the room temperature [2]. The processes of electrochemical deposition of Sn and Pd were performed with amounts of the electricity of 400 and 267 mC, giving an atomic ratio synthesized the electrocatalysts of 60 at.% Sn‒40 at.% Pd. Morpological and elemental analysis of the Sn-Pd electrocatalysts was performed by scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS), respectively. The particle size distribution (PSD) was used to estimate a size of Sn dendrites. The electrocatalytic activity of synthesized Sn-Pd electrocatalysts towards ethanol oxidation reaction (EOR) was examined by cyclic voltammetry (CV) and chronoamperometry, using 1 M NaOH with 1 M ethanol solution. Depending on the applied cathodic potentials, the following shapes of dendrites were formed: the needle-like and the spear-like at –1200 mV, an individual fern-like dendrites at –1400 mV, and intertwined network of dendrites of the fern-like shape was formed at cathodic potentials of – 1600 and – 1800 mV vs. Ag/AgCl. The partial coverage of the Sn dendrites through a formation of compact islands of Pd was attained by electrodeposition of Pd on the electrodes with the Sn dendrites. It was shown that morphology of the Sn dendrites as sub-layer strongly correlates the electrocatalytic activity of the Sn-Pd electrocatalysts. The largest electrocatalytic activity showed the Sn- Pd electrocatalyst constructed from the individual fern-like Sn dendrites as sub-layer produced at a cathodic potential of –1400 mV vs. Ag/AgCl. This dendrite type showed more branchy morphology than those obtained at the other cathodic potentials. Formation of individual needle-like and spear-like dendrites at a cathodic potential of – 1200 mV vs. Ag/AgCl did not contribute significantly to the increase of the initial electrode surface area. On the other hand, due to a high nucleation rate, growth a dendrites from one nucleation centre in more directions, as well as the fact that Sn is very soft metal, morphological characteristics of intertwined Sn dendrites obtained at cathodic potentials of −1600 and −1800 mV vs. Ag/AgCl approached to those of compact massive Sn. The partial coverage of Sn dendrites by Pd can be attributed to the current density distribution effect, because Pd electrodeposition process preferentially perfomed at the higher parts of the surface area, i.e. on the dendrites, rather than on the flat part of the electrode surface area. Aside from an influence of degree of branchy of Sn dendrites as sub-layer on the electrocatalytic activity in ethanol oxidation, varios electrocatalytic performance of the Sn-Pd electrocatalysts can also be ascribed to the bifunctional effect.",
publisher = "Lausanne : International Society of Electrochemistry",
journal = "The 74th Annual Meeting of the International Society of Electrochemistry : Bridging Scientific Disciplines to Address the World’s Challenges 3 to 8 September 2023. Lyon, France",
title = "Influence of Morphology of Sn Dendrites as Sub-layer on Electrolcatalytic Performance of Sn-Pd Electrocatalysts",
url = "https://hdl.handle.net/21.15107/rcub_dais_15643"
}

Nikolić, N. D., Lović, J., Ignjatović, N.,& Dimitrijević, S. B.. (2023). Influence of Morphology of Sn Dendrites as Sub-layer on Electrolcatalytic Performance of Sn-Pd Electrocatalysts. in The 74th Annual Meeting of the International Society of Electrochemistry : Bridging Scientific Disciplines to Address the World’s Challenges 3 to 8 September 2023. Lyon, France
Lausanne : International Society of Electrochemistry..
https://hdl.handle.net/21.15107/rcub_dais_15643

Nikolić ND, Lović J, Ignjatović N, Dimitrijević SB. Influence of Morphology of Sn Dendrites as Sub-layer on Electrolcatalytic Performance of Sn-Pd Electrocatalysts. in The 74th Annual Meeting of the International Society of Electrochemistry : Bridging Scientific Disciplines to Address the World’s Challenges 3 to 8 September 2023. Lyon, France. 2023;.
https://hdl.handle.net/21.15107/rcub_dais_15643 .

Nikolić, Nebojša D., Lović, Jelena, Ignjatović, Nenad, Dimitrijević, Silvana B., "Influence of Morphology of Sn Dendrites as Sub-layer on Electrolcatalytic Performance of Sn-Pd Electrocatalysts" in The 74th Annual Meeting of the International Society of Electrochemistry : Bridging Scientific Disciplines to Address the World’s Challenges 3 to 8 September 2023. Lyon, France (2023),
https://hdl.handle.net/21.15107/rcub_dais_15643 .

TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15642
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that are easy to obtain from any kind of discarded teeth and have the ability to differentiate into several cell types which makes them very popular in researches regarding regenerative dentistry. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry and they become very attractive tool for the treatment of bone tissue defects but also as a carriers of bioactive molecules. Before clinical application, thorough preclinical studies should be conducted regarding biocompatibility and biofunctionality of synthesized nanomaterials. Therefore, the aim of this study was to examine the influence of synthesized nanomaterial, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), on the proliferation of DPSCs during osteogenic differentiation in cell culture in vitro. We isolated the cells from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were incubated with two concentrations of CP/PLGA nanoparticles in standard cell culture media and media for osteogenic differentiation. Cell proliferation rate was assessed using MTT and Crystal violet test after one, three and seven days of cell culturing. The results showed time- and concentration-dependent differences in DPSCs proliferation in the presence of nanomaterial. Differences in cell proliferation were noticed between osteogenic and standard cell culture media as well as in results obtained by performed tests. Increased cell proliferation was observed over time in both media but this effect was dependent on nanomaterial concentration. Calcium phosphate-based nanomaterials and DPSCs, based on observed cell materials interactions, could be a promising tool in bone tissue regeneration and further studies using in vitro as well as in vivo models should be conducted to fully unravel all of its potential.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_dais_15642
ER  - 

@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2023",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that are easy to obtain from any kind of discarded teeth and have the ability to differentiate into several cell types which makes them very popular in researches regarding regenerative dentistry. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry and they become very attractive tool for the treatment of bone tissue defects but also as a carriers of bioactive molecules. Before clinical application, thorough preclinical studies should be conducted regarding biocompatibility and biofunctionality of synthesized nanomaterials. Therefore, the aim of this study was to examine the influence of synthesized nanomaterial, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), on the proliferation of DPSCs during osteogenic differentiation in cell culture in vitro. We isolated the cells from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were incubated with two concentrations of CP/PLGA nanoparticles in standard cell culture media and media for osteogenic differentiation. Cell proliferation rate was assessed using MTT and Crystal violet test after one, three and seven days of cell culturing. The results showed time- and concentration-dependent differences in DPSCs proliferation in the presence of nanomaterial. Differences in cell proliferation were noticed between osteogenic and standard cell culture media as well as in results obtained by performed tests. Increased cell proliferation was observed over time in both media but this effect was dependent on nanomaterial concentration. Calcium phosphate-based nanomaterials and DPSCs, based on observed cell materials interactions, could be a promising tool in bone tissue regeneration and further studies using in vitro as well as in vivo models should be conducted to fully unravel all of its potential.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_dais_15642"
}

Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2023). Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 11-11.
https://hdl.handle.net/21.15107/rcub_dais_15642

Tomić M, Stojanović S, Ignjatović N, Najman S. Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:11-11.
https://hdl.handle.net/21.15107/rcub_dais_15642 .

Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):11-11,
https://hdl.handle.net/21.15107/rcub_dais_15642 .

TY  - JOUR
AU  - Pantović-Pavlović, Marijana R.
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Mirkov, Ivana I.
AU  - Kulaš, Jelena B.
AU  - Malešević, Anastasija Lj.
AU  - Pavlović, Miroslav M.
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14406
AB  - The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants
SP  - 227
VL  - 14
IS  - 4
DO  - 10.3390/jfb14040227
UR  - https://hdl.handle.net/21.15107/rcub_dais_14406
ER  - 

@article{
author = "Pantović-Pavlović, Marijana R. and Ignjatović, Nenad and Panić, Vladimir V. and Mirkov, Ivana I. and Kulaš, Jelena B. and Malešević, Anastasija Lj. and Pavlović, Miroslav M.",
year = "2023",
abstract = "The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants",
pages = "227",
volume = "14",
number = "4",
doi = "10.3390/jfb14040227",
url = "https://hdl.handle.net/21.15107/rcub_dais_14406"
}

Pantović-Pavlović, M. R., Ignjatović, N., Panić, V. V., Mirkov, I. I., Kulaš, J. B., Malešević, A. Lj.,& Pavlović, M. M.. (2023). Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials
Basel : MDPI AG., 14(4), 227.
https://doi.org/10.3390/jfb14040227
https://hdl.handle.net/21.15107/rcub_dais_14406

Pantović-Pavlović MR, Ignjatović N, Panić VV, Mirkov II, Kulaš JB, Malešević AL, Pavlović MM. Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials. 2023;14(4):227.
doi:10.3390/jfb14040227
https://hdl.handle.net/21.15107/rcub_dais_14406 .

Pantović-Pavlović, Marijana R., Ignjatović, Nenad, Panić, Vladimir V., Mirkov, Ivana I., Kulaš, Jelena B., Malešević, Anastasija Lj., Pavlović, Miroslav M., "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants" in Journal of Functional Biomaterials, 14, no. 4 (2023):227,
https://doi.org/10.3390/jfb14040227 .,
https://hdl.handle.net/21.15107/rcub_dais_14406 .

TY  - JOUR
AU  - Marković, Smilja
AU  - Rau, Julietta V.
AU  - De Bonis, Angela
AU  - De Bellis, Giovanni
AU  - Stojanović, Zoran
AU  - Veselinović, Ljiljana
AU  - Mitrić, Miodrag
AU  - Ignjatović, Nenad
AU  - Škapin, Srečo Davor
AU  - Vengust, Damjan
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/12953
AB  - Dense calcium phosphate-based ceramics were fabricated to be used as targets for pulsed laser deposition (PLD). Nanostructured cobalt-substituted hydroxyapatite (Co:HAP) was used as a starting powder. To vary phase composition and microstructure of targets, two sintering approaches were applied, conventional (CS) and two-step sintering (TSS). The obtained results show that in both cases biphasic calcium phosphate (BCP) ceramics (targets) were prepared, with slightly different HAP-to-β–TCP amount ratio and a significantly different microstructure. While the CS method yielded fully dense ceramics with an average grain size of 1.3 μm, the ceramics prepared by TSS had a density of 98.5%, with a predominant grain size below 100 nm. (Ca + Co)P coatings were prepared by PLD of (Ca + Co)P targets. The temperature of the Ti substrate was adjusted to be 25 and 500 °C. The results show that the phase composition of (Ca + Co)P coatings depended on the phase composition of targets as well as on the temperature of the Ti substrate. The coating prepared at 25 °C using CS target consisted of three calcium phosphate phases, HAP, β–TCP and α–TCP; when the TSS target was used, the coating was biphasic, containing HAP and β–TCP. When the substrate was heated to 500 °C, regardless of whether the CS or the TSS target was used, the deposited coatings were composed of HAP and α–TCP. Due to different phase compositions, the (Ca + Co)P coatings deposited at 25 °C showed an improved hardness compared to those deposited at 500 °C. The obtained results confirmed that the phase composition, morphology and mechanical properties of 0.3 μm thick (Ca + Co)P coatings on a Ti substrate can be tailored by employing (Ca + Co)P targets with different microstructures, and also by varying the temperature of the Ti substrate during deposition experiments.
T2  - Surface and Coatings Technology
T2  - Surface and Coatings TechnologySurface and Coatings Technology
T1  - Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium
SP  - 128275
VL  - 437
DO  - 10.1016/j.surfcoat.2022.128275
UR  - https://hdl.handle.net/21.15107/rcub_dais_12953
ER  - 

@article{
author = "Marković, Smilja and Rau, Julietta V. and De Bonis, Angela and De Bellis, Giovanni and Stojanović, Zoran and Veselinović, Ljiljana and Mitrić, Miodrag and Ignjatović, Nenad and Škapin, Srečo Davor and Vengust, Damjan",
year = "2022",
abstract = "Dense calcium phosphate-based ceramics were fabricated to be used as targets for pulsed laser deposition (PLD). Nanostructured cobalt-substituted hydroxyapatite (Co:HAP) was used as a starting powder. To vary phase composition and microstructure of targets, two sintering approaches were applied, conventional (CS) and two-step sintering (TSS). The obtained results show that in both cases biphasic calcium phosphate (BCP) ceramics (targets) were prepared, with slightly different HAP-to-β–TCP amount ratio and a significantly different microstructure. While the CS method yielded fully dense ceramics with an average grain size of 1.3 μm, the ceramics prepared by TSS had a density of 98.5%, with a predominant grain size below 100 nm. (Ca + Co)P coatings were prepared by PLD of (Ca + Co)P targets. The temperature of the Ti substrate was adjusted to be 25 and 500 °C. The results show that the phase composition of (Ca + Co)P coatings depended on the phase composition of targets as well as on the temperature of the Ti substrate. The coating prepared at 25 °C using CS target consisted of three calcium phosphate phases, HAP, β–TCP and α–TCP; when the TSS target was used, the coating was biphasic, containing HAP and β–TCP. When the substrate was heated to 500 °C, regardless of whether the CS or the TSS target was used, the deposited coatings were composed of HAP and α–TCP. Due to different phase compositions, the (Ca + Co)P coatings deposited at 25 °C showed an improved hardness compared to those deposited at 500 °C. The obtained results confirmed that the phase composition, morphology and mechanical properties of 0.3 μm thick (Ca + Co)P coatings on a Ti substrate can be tailored by employing (Ca + Co)P targets with different microstructures, and also by varying the temperature of the Ti substrate during deposition experiments.",
journal = "Surface and Coatings Technology, Surface and Coatings TechnologySurface and Coatings Technology",
title = "Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium",
pages = "128275",
volume = "437",
doi = "10.1016/j.surfcoat.2022.128275",
url = "https://hdl.handle.net/21.15107/rcub_dais_12953"
}

Marković, S., Rau, J. V., De Bonis, A., De Bellis, G., Stojanović, Z., Veselinović, L., Mitrić, M., Ignjatović, N., Škapin, S. D.,& Vengust, D.. (2022). Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium. in Surface and Coatings Technology, 437, 128275.
https://doi.org/10.1016/j.surfcoat.2022.128275
https://hdl.handle.net/21.15107/rcub_dais_12953

Marković S, Rau JV, De Bonis A, De Bellis G, Stojanović Z, Veselinović L, Mitrić M, Ignjatović N, Škapin SD, Vengust D. Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium. in Surface and Coatings Technology. 2022;437:128275.
doi:10.1016/j.surfcoat.2022.128275
https://hdl.handle.net/21.15107/rcub_dais_12953 .

Marković, Smilja, Rau, Julietta V., De Bonis, Angela, De Bellis, Giovanni, Stojanović, Zoran, Veselinović, Ljiljana, Mitrić, Miodrag, Ignjatović, Nenad, Škapin, Srečo Davor, Vengust, Damjan, "Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium" in Surface and Coatings Technology, 437 (2022):128275,
https://doi.org/10.1016/j.surfcoat.2022.128275 .,
https://hdl.handle.net/21.15107/rcub_dais_12953 .

TY  - JOUR
AU  - Marković, Smilja
AU  - Rau, Julietta V.
AU  - De Bonis, Angela
AU  - De Bellis, Giovanni
AU  - Stojanović, Zoran
AU  - Veselinović, Ljiljana
AU  - Mitrić, Miodrag
AU  - Ignjatović, Nenad
AU  - Škapin, Srečo Davor
AU  - Vengust, Damjan
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13034
AB  - Dense calcium phosphate-based ceramics were fabricated to be used as targets for pulsed laser deposition (PLD). Nanostructured cobalt-substituted hydroxyapatite (Co:HAP) was used as a starting powder. To vary phase composition and microstructure of targets, two sintering approaches were applied, conventional (CS) and two-step sintering (TSS). The obtained results show that in both cases biphasic calcium phosphate (BCP) ceramics (targets) were prepared, with slightly different HAP-to-β–TCP amount ratio and a significantly different microstructure. While the CS method yielded fully dense ceramics with an average grain size of 1.3 μm, the ceramics prepared by TSS had a density of 98.5%, with a predominant grain size below 100 nm. (Ca + Co)P coatings were prepared by PLD of (Ca + Co)P targets. The temperature of the Ti substrate was adjusted to be 25 and 500 °C. The results show that the phase composition of (Ca + Co)P coatings depended on the phase composition of targets as well as on the temperature of the Ti substrate. The coating prepared at 25 °C using CS target consisted of three calcium phosphate phases, HAP, β–TCP and α–TCP; when the TSS target was used, the coating was biphasic, containing HAP and β–TCP. When the substrate was heated to 500 °C, regardless of whether the CS or the TSS target was used, the deposited coatings were composed of HAP and α–TCP. Due to different phase compositions, the (Ca + Co)P coatings deposited at 25 °C showed an improved hardness compared to those deposited at 500 °C. The obtained results confirmed that the phase composition, morphology and mechanical properties of 0.3 μm thick (Ca + Co)P coatings on a Ti substrate can be tailored by employing (Ca + Co)P targets with different microstructures, and also by varying the temperature of the Ti substrate during deposition experiments.
T2  - Surface and Coatings Technology
T1  - Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium
SP  - 128275
VL  - 437
DO  - 10.1016/j.surfcoat.2022.128275
UR  - https://hdl.handle.net/21.15107/rcub_dais_13034
ER  - 

@article{
author = "Marković, Smilja and Rau, Julietta V. and De Bonis, Angela and De Bellis, Giovanni and Stojanović, Zoran and Veselinović, Ljiljana and Mitrić, Miodrag and Ignjatović, Nenad and Škapin, Srečo Davor and Vengust, Damjan",
year = "2022",
abstract = "Dense calcium phosphate-based ceramics were fabricated to be used as targets for pulsed laser deposition (PLD). Nanostructured cobalt-substituted hydroxyapatite (Co:HAP) was used as a starting powder. To vary phase composition and microstructure of targets, two sintering approaches were applied, conventional (CS) and two-step sintering (TSS). The obtained results show that in both cases biphasic calcium phosphate (BCP) ceramics (targets) were prepared, with slightly different HAP-to-β–TCP amount ratio and a significantly different microstructure. While the CS method yielded fully dense ceramics with an average grain size of 1.3 μm, the ceramics prepared by TSS had a density of 98.5%, with a predominant grain size below 100 nm. (Ca + Co)P coatings were prepared by PLD of (Ca + Co)P targets. The temperature of the Ti substrate was adjusted to be 25 and 500 °C. The results show that the phase composition of (Ca + Co)P coatings depended on the phase composition of targets as well as on the temperature of the Ti substrate. The coating prepared at 25 °C using CS target consisted of three calcium phosphate phases, HAP, β–TCP and α–TCP; when the TSS target was used, the coating was biphasic, containing HAP and β–TCP. When the substrate was heated to 500 °C, regardless of whether the CS or the TSS target was used, the deposited coatings were composed of HAP and α–TCP. Due to different phase compositions, the (Ca + Co)P coatings deposited at 25 °C showed an improved hardness compared to those deposited at 500 °C. The obtained results confirmed that the phase composition, morphology and mechanical properties of 0.3 μm thick (Ca + Co)P coatings on a Ti substrate can be tailored by employing (Ca + Co)P targets with different microstructures, and also by varying the temperature of the Ti substrate during deposition experiments.",
journal = "Surface and Coatings Technology",
title = "Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium",
pages = "128275",
volume = "437",
doi = "10.1016/j.surfcoat.2022.128275",
url = "https://hdl.handle.net/21.15107/rcub_dais_13034"
}

Marković, S., Rau, J. V., De Bonis, A., De Bellis, G., Stojanović, Z., Veselinović, L., Mitrić, M., Ignjatović, N., Škapin, S. D.,& Vengust, D.. (2022). Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium. in Surface and Coatings Technology, 437, 128275.
https://doi.org/10.1016/j.surfcoat.2022.128275
https://hdl.handle.net/21.15107/rcub_dais_13034

Marković S, Rau JV, De Bonis A, De Bellis G, Stojanović Z, Veselinović L, Mitrić M, Ignjatović N, Škapin SD, Vengust D. Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium. in Surface and Coatings Technology. 2022;437:128275.
doi:10.1016/j.surfcoat.2022.128275
https://hdl.handle.net/21.15107/rcub_dais_13034 .

Marković, Smilja, Rau, Julietta V., De Bonis, Angela, De Bellis, Giovanni, Stojanović, Zoran, Veselinović, Ljiljana, Mitrić, Miodrag, Ignjatović, Nenad, Škapin, Srečo Davor, Vengust, Damjan, "Pathway to tailor the phase composition, microstructure and mechanical properties of pulsed laser deposited cobalt-substituted calcium phosphate coatings on titanium" in Surface and Coatings Technology, 437 (2022):128275,
https://doi.org/10.1016/j.surfcoat.2022.128275 .,
https://hdl.handle.net/21.15107/rcub_dais_13034 .

TY  - CONF
AU  - Vuković, Marina
AU  - Dorm, Bruna Carolina
AU  - Trovatti, Eliane
AU  - Ignjatović, Nenad
AU  - Marković, Smilja
AU  - Škapin, Srečo Davor
AU  - Dinić, Ivana
AU  - Mančić, Lidija
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13630
AB  - Hydroxyapatite (HAp) attracts great attention due to application in reconstructive medicine for hard tissues, mostly bones and teeth, where it is declared to be highly biocompatible material. Its grafting with amino acids further increases biocompatibility and has crucial importance for acceptance of body implants. In this work different methods of grafting were investigated: simple mixing, thermal treatment induction and in situ synthesis/grafting reactions. Two amino acid precursors were separately tested in grafting procedures: pure alanine and alanine methyl ester hydrochloride. The efficiency of grafting was determined based on X-ray powder diffraction (XRPD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and thermal analyses (DSC, TG/DTA) of obtained powders, while complementary UV-VIS spectroscopy of supernatants was additionally performed for quantitative determination of non-grafted nitrogen using ninhydrin standardized procedure.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application X New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 26-27. September 2022.
T1  - Hydroxyapatite grafting with alanine amino acid - efficiency of different methods
SP  - 58
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_dais_13630
ER  - 

@conference{
author = "Vuković, Marina and Dorm, Bruna Carolina and Trovatti, Eliane and Ignjatović, Nenad and Marković, Smilja and Škapin, Srečo Davor and Dinić, Ivana and Mančić, Lidija",
year = "2022",
abstract = "Hydroxyapatite (HAp) attracts great attention due to application in reconstructive medicine for hard tissues, mostly bones and teeth, where it is declared to be highly biocompatible material. Its grafting with amino acids further increases biocompatibility and has crucial importance for acceptance of body implants. In this work different methods of grafting were investigated: simple mixing, thermal treatment induction and in situ synthesis/grafting reactions. Two amino acid precursors were separately tested in grafting procedures: pure alanine and alanine methyl ester hydrochloride. The efficiency of grafting was determined based on X-ray powder diffraction (XRPD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and thermal analyses (DSC, TG/DTA) of obtained powders, while complementary UV-VIS spectroscopy of supernatants was additionally performed for quantitative determination of non-grafted nitrogen using ninhydrin standardized procedure.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application X New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 26-27. September 2022.",
title = "Hydroxyapatite grafting with alanine amino acid - efficiency of different methods",
pages = "58-59",
url = "https://hdl.handle.net/21.15107/rcub_dais_13630"
}

Vuković, M., Dorm, B. C., Trovatti, E., Ignjatović, N., Marković, S., Škapin, S. D., Dinić, I.,& Mančić, L.. (2022). Hydroxyapatite grafting with alanine amino acid - efficiency of different methods. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application X New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 26-27. September 2022.
Belgrade : Serbian Ceramic Society., 58-59.
https://hdl.handle.net/21.15107/rcub_dais_13630

Vuković M, Dorm BC, Trovatti E, Ignjatović N, Marković S, Škapin SD, Dinić I, Mančić L. Hydroxyapatite grafting with alanine amino acid - efficiency of different methods. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application X New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 26-27. September 2022.. 2022;:58-59.
https://hdl.handle.net/21.15107/rcub_dais_13630 .

Vuković, Marina, Dorm, Bruna Carolina, Trovatti, Eliane, Ignjatović, Nenad, Marković, Smilja, Škapin, Srečo Davor, Dinić, Ivana, Mančić, Lidija, "Hydroxyapatite grafting with alanine amino acid - efficiency of different methods" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application X New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, 26-27. September 2022. (2022):58-59,
https://hdl.handle.net/21.15107/rcub_dais_13630 .

TY  - CONF
AU  - Živković, Jelena M.
AU  - Radenković, Milena
AU  - Stojanović, Sanja
AU  - Najdanović, Jelena
AU  - Cvetković, Vladimir
AU  - Vukelić Nikolić, Marija
AU  - Vučković, Ivica
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2022
UR  - http://informatikaubiomedicini.ekonferencije.com/presentation/paperpresentation/679/6441
UR  - https://dais.sanu.ac.rs/123456789/13655
AB  - Данашњи приступ репарацији и регенерацији ткива често подразумева употребу различитих биоматеријала, самостално или у комбинацији са ћелијама и/или факторима раста. Сви материјали, без обзира да ли су природног порекла или синтетисани, изазивају реакцију околног ткива након имплантације. Ова реакција подразумева низ повезаних догађаја од којих коначно зависи да ли ће имплантирани материјал бити добро прихваћен и обавити своју примарну улогу. Сваки материјал за организам представља страно тело и иницира пролазну инфламаторну реакцију, чија дужина трајања и интензитет умногоме одређују његову даљу судбину. На то утиче хемијски састав материјала, величина и облик његових гранула, порозност, компактност, као и степен биодеградабилности. Тако, судбина материјала може кренути једним од два пута: ка доброј интеграцији са околним ткивом, подршци ћелијама са којима интерагује и, код биодеградабилних материјала, замени здравим, функционалним ткивом, или ће пак изазвати снажан и продужен инфламаторни одговор који резултује потпуном изолацијом материјала од остатка ткива дебелом фиброзном капсулом. In vivo испитивања одговора ткива на имплантирани биоматеријал подразумевају истраживања на различитим анималним моделима и у различитим ткивима, са ортопичним или ектопичним имплантацијама. У овом раду ће бити дат преглед наших in vivo истраживања на различитим моделима имплантација. Користили смо ортопичне моделе за формирање кости код пацова и кунића и моделе супкутаних имплантација различитих биоматеријала мишевима и пацовима. За анализу импланата и околног ткива у временским периодима раног и касног одговора коришћене су бројне методе као што су хистолошка бојења, хистоморфометрија, имунохистохемија, СЕМ, радиографске методе, анализа специфичне експресије гена и друге. In vivo анимални модели у сврху претклиничких испитивања важни су за добијање смерница за клиничку примену.
AB  - Today's approach to tissue repair and regeneration often involves application of different biomaterials, alone or in combination with cells and/or growth factors. All materials, regardless of whether they are of natural origin or synthesized, cause a reaction in the surrounding tissue after implantation. This reaction involves a series of related events on which ultimately depends whether the implanted material will be well accepted and perform its primary role. Each material represents a foreign body for the organism and initiates a transient inflammatory reaction, the duration and intensity of which largely determine materil's further fate. Inflammatory reaction is influenced by material's chemical composition, the size and shape of its granules, porosity, compactness, as well as the degree of material's biodegradability. Thus, the fate of the material can go one of two ways: towards good integration with the surrounding tissue, supporting the cells with which it interacts and, in the case of biodegradable materials, replacement by healthy, functional tissue, or it will cause a strong inflammatory response resulting in the complete isolation of the material from the rest of the tissue with thick fibrous capsule. In vivo research of tissue response to implanted biomaterial involves investigation in different animal models and in different tissues, with orthotopic or ectopic implantations. We used orthotopic models for bone formation in rats and rabbits and models of subcutaneous implantation of various biomaterials in mice and rats. Numerous methods were used for the analysis of implants and surrounding tissue in the time periods of early and late response, such as histological staining, histomorphometry, immunohistochemistry, SEM, radiographic methods, analysis of specific gene expression and others. In vivo animal models for the purpose of preclinical studies are important in order to obtain guidelines for clinical application.
PB  - Banja Luka : ANURS
C3  - Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
T1  - Одговор ткива на различите имплантиране биоматеријале у моделима in vivo
T1  - Tissue response to different implanted biomaterials in models in vivo
UR  - https://hdl.handle.net/21.15107/rcub_dais_13655
ER  - 

@conference{
author = "Živković, Jelena M. and Radenković, Milena and Stojanović, Sanja and Najdanović, Jelena and Cvetković, Vladimir and Vukelić Nikolić, Marija and Vučković, Ivica and Ignjatović, Nenad and Najman, Stevo",
year = "2022",
abstract = "Данашњи приступ репарацији и регенерацији ткива често подразумева употребу различитих биоматеријала, самостално или у комбинацији са ћелијама и/или факторима раста. Сви материјали, без обзира да ли су природног порекла или синтетисани, изазивају реакцију околног ткива након имплантације. Ова реакција подразумева низ повезаних догађаја од којих коначно зависи да ли ће имплантирани материјал бити добро прихваћен и обавити своју примарну улогу. Сваки материјал за организам представља страно тело и иницира пролазну инфламаторну реакцију, чија дужина трајања и интензитет умногоме одређују његову даљу судбину. На то утиче хемијски састав материјала, величина и облик његових гранула, порозност, компактност, као и степен биодеградабилности. Тако, судбина материјала може кренути једним од два пута: ка доброј интеграцији са околним ткивом, подршци ћелијама са којима интерагује и, код биодеградабилних материјала, замени здравим, функционалним ткивом, или ће пак изазвати снажан и продужен инфламаторни одговор који резултује потпуном изолацијом материјала од остатка ткива дебелом фиброзном капсулом. In vivo испитивања одговора ткива на имплантирани биоматеријал подразумевају истраживања на различитим анималним моделима и у различитим ткивима, са ортопичним или ектопичним имплантацијама. У овом раду ће бити дат преглед наших in vivo истраживања на различитим моделима имплантација. Користили смо ортопичне моделе за формирање кости код пацова и кунића и моделе супкутаних имплантација различитих биоматеријала мишевима и пацовима. За анализу импланата и околног ткива у временским периодима раног и касног одговора коришћене су бројне методе као што су хистолошка бојења, хистоморфометрија, имунохистохемија, СЕМ, радиографске методе, анализа специфичне експресије гена и друге. In vivo анимални модели у сврху претклиничких испитивања важни су за добијање смерница за клиничку примену., Today's approach to tissue repair and regeneration often involves application of different biomaterials, alone or in combination with cells and/or growth factors. All materials, regardless of whether they are of natural origin or synthesized, cause a reaction in the surrounding tissue after implantation. This reaction involves a series of related events on which ultimately depends whether the implanted material will be well accepted and perform its primary role. Each material represents a foreign body for the organism and initiates a transient inflammatory reaction, the duration and intensity of which largely determine materil's further fate. Inflammatory reaction is influenced by material's chemical composition, the size and shape of its granules, porosity, compactness, as well as the degree of material's biodegradability. Thus, the fate of the material can go one of two ways: towards good integration with the surrounding tissue, supporting the cells with which it interacts and, in the case of biodegradable materials, replacement by healthy, functional tissue, or it will cause a strong inflammatory response resulting in the complete isolation of the material from the rest of the tissue with thick fibrous capsule. In vivo research of tissue response to implanted biomaterial involves investigation in different animal models and in different tissues, with orthotopic or ectopic implantations. We used orthotopic models for bone formation in rats and rabbits and models of subcutaneous implantation of various biomaterials in mice and rats. Numerous methods were used for the analysis of implants and surrounding tissue in the time periods of early and late response, such as histological staining, histomorphometry, immunohistochemistry, SEM, radiographic methods, analysis of specific gene expression and others. In vivo animal models for the purpose of preclinical studies are important in order to obtain guidelines for clinical application.",
publisher = "Banja Luka : ANURS",
journal = "Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.",
title = "Одговор ткива на различите имплантиране биоматеријале у моделима in vivo, Tissue response to different implanted biomaterials in models in vivo",
url = "https://hdl.handle.net/21.15107/rcub_dais_13655"
}

Živković, J. M., Radenković, M., Stojanović, S., Najdanović, J., Cvetković, V., Vukelić Nikolić, M., Vučković, I., Ignjatović, N.,& Najman, S.. (2022). Одговор ткива на различите имплантиране биоматеријале у моделима in vivo. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
Banja Luka : ANURS..
https://hdl.handle.net/21.15107/rcub_dais_13655

Živković JM, Radenković M, Stojanović S, Najdanović J, Cvetković V, Vukelić Nikolić M, Vučković I, Ignjatović N, Najman S. Одговор ткива на различите имплантиране биоматеријале у моделима in vivo. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.. 2022;.
https://hdl.handle.net/21.15107/rcub_dais_13655 .

Živković, Jelena M., Radenković, Milena, Stojanović, Sanja, Najdanović, Jelena, Cvetković, Vladimir, Vukelić Nikolić, Marija, Vučković, Ivica, Ignjatović, Nenad, Najman, Stevo, "Одговор ткива на различите имплантиране биоматеријале у моделима in vivo" in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022. (2022),
https://hdl.handle.net/21.15107/rcub_dais_13655 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Najdanović, Jelena
AU  - Mitić, Žarko
AU  - Vasiljević, Perica
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2022
UR  - http://informatikaubiomedicini.ekonferencije.com/presentation/paperpresentation/679/6440
UR  - https://dais.sanu.ac.rs/123456789/13654
AB  - Тестирање биоматеријала намењених за примену у регенеративној медицини на ћелијским моделима in vitro је неопходан први корак у циљу процене биокомпатибилности биоматеријала али и њихове биофункционалности. Постоје различити начини тестирања биоматеријала, као и различити ћелијски модели на којима се врши тестирање. Одабир адекватног ћелијског модела врши се на основу врсте биоматеријала и његове потенцијалне примене а тестирање се врши најпре стандардним тестовима као што су тестови цитотоксичности и генотоксичности а затим се испитују друге биолошке карактеристике као што су нпр. способност биоматеријала да индукује диференцијацију ћелија у одређени тип или способност активације макрофага ка одређеном фенотипу и индукција њиховог одговора ин витро, и многе друге. У овом раду ће бити преглед резултата наших досадашњих истраживања у области карактеризације биоматеријала и испитивања на ћелијским моделима in vitro. Испитивали смо биоматеријале намењене за регенерацију тврдих ткива, нпр. материјали на бази хидроксиапатита, калцијум фосфата, природног и синтетског порекла, тако и биоматеријале за регенерацију меких ткива, нпр. материјали на бази колагена или других полимера како природног тако и синтетског порекла. Да би се побољшала биофункционалност биоматеријала често им се додају биоактивне супстанце или њихове комбинације, а материјали могу бити у различитим облицима, у виду скафолда, у виду гранула, нано- или микро- димензија, мембрана и разним другим, при чему облик материјала утиче и на одабир ћелијског модела који се користи за тестирање. За карактеризацију биматеријала користили смо методе као што су SEM, EDX, FTIR и друге. Рађени су и тестови цитотоксичности, генотоксичности, пролиферације, миграције, диференцијације, фагоцитозе и многи други, а биоматеријале смо тестирали у директном контакту са ћелијама и у индиректном контакту. Испитивали смо и промене биоматеријала након интеракције са ћелијама као и понашање биоматеријала у различитим условима in vitro.
AB  - Testing biomaterials intended for use in regenerative medicine on cell models in vitro is a necessary first step in order to assess the biocompatibility of biomaterials as well as their biofunctionality. There are different methods of testing biomaterials, as well as different cell models on which testing is performed. The selection of an adequate cell model is made based on the type of biomaterial and its potential application, and testing is performed first using standard tests such as cytotoxicity and genotoxicity tests, and then other biological characteristics are examined such as, for example, the ability of biomaterials to induce the differentiation of cells into a certain type or the ability to activate macrophages towards a certain phenotype and the induction of their response in vitro, and many others. This paper will present the results of our research in the field of characterization of biomaterials and examination on cell models in vitro. We examined biomaterials intended for the regeneration of hard tissues, e.g. materials based on hydroxyapatite, calcium phosphate, of natural and synthetic origin, as well as biomaterials for the regeneration of soft tissues, e.g. materials based on collagen or other polymers of both natural and synthetic origin. In order to improve the biofunctionality of biomaterials, bioactive substances or their combinations are often added, and the biomaterials can be in different forms, in the form of scaffolds, in the form of granules, nano- or micro-dimensions, membranes and many others, whereby the shape of the material also affects the selection of the cell model used for testing. To characterize bimaterials, we used methods such as SEM, EDX, FTIR and others. In addition to cytotoxicity and genotoxicity tests, we performed proliferation, migration, differentiation, phagocytosis and many other tests, and we tested biomaterials both in direct contact with cells and in indirect contact. We also examined changes in biomaterials after interaction with cells, as well as the behavior of biomaterials in various conditions in vitro.
PB  - Banja Luka : ANURS
C3  - Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
T1  - Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације
T1  - Interaction of cells with different biomaterials in vitro and methods of characterization
UR  - https://hdl.handle.net/21.15107/rcub_dais_13654
ER  - 

@conference{
author = "Stojanović, Sanja and Najdanović, Jelena and Mitić, Žarko and Vasiljević, Perica and Ignjatović, Nenad and Najman, Stevo",
year = "2022",
abstract = "Тестирање биоматеријала намењених за примену у регенеративној медицини на ћелијским моделима in vitro је неопходан први корак у циљу процене биокомпатибилности биоматеријала али и њихове биофункционалности. Постоје различити начини тестирања биоматеријала, као и различити ћелијски модели на којима се врши тестирање. Одабир адекватног ћелијског модела врши се на основу врсте биоматеријала и његове потенцијалне примене а тестирање се врши најпре стандардним тестовима као што су тестови цитотоксичности и генотоксичности а затим се испитују друге биолошке карактеристике као што су нпр. способност биоматеријала да индукује диференцијацију ћелија у одређени тип или способност активације макрофага ка одређеном фенотипу и индукција њиховог одговора ин витро, и многе друге. У овом раду ће бити преглед резултата наших досадашњих истраживања у области карактеризације биоматеријала и испитивања на ћелијским моделима in vitro. Испитивали смо биоматеријале намењене за регенерацију тврдих ткива, нпр. материјали на бази хидроксиапатита, калцијум фосфата, природног и синтетског порекла, тако и биоматеријале за регенерацију меких ткива, нпр. материјали на бази колагена или других полимера како природног тако и синтетског порекла. Да би се побољшала биофункционалност биоматеријала често им се додају биоактивне супстанце или њихове комбинације, а материјали могу бити у различитим облицима, у виду скафолда, у виду гранула, нано- или микро- димензија, мембрана и разним другим, при чему облик материјала утиче и на одабир ћелијског модела који се користи за тестирање. За карактеризацију биматеријала користили смо методе као што су SEM, EDX, FTIR и друге. Рађени су и тестови цитотоксичности, генотоксичности, пролиферације, миграције, диференцијације, фагоцитозе и многи други, а биоматеријале смо тестирали у директном контакту са ћелијама и у индиректном контакту. Испитивали смо и промене биоматеријала након интеракције са ћелијама као и понашање биоматеријала у различитим условима in vitro., Testing biomaterials intended for use in regenerative medicine on cell models in vitro is a necessary first step in order to assess the biocompatibility of biomaterials as well as their biofunctionality. There are different methods of testing biomaterials, as well as different cell models on which testing is performed. The selection of an adequate cell model is made based on the type of biomaterial and its potential application, and testing is performed first using standard tests such as cytotoxicity and genotoxicity tests, and then other biological characteristics are examined such as, for example, the ability of biomaterials to induce the differentiation of cells into a certain type or the ability to activate macrophages towards a certain phenotype and the induction of their response in vitro, and many others. This paper will present the results of our research in the field of characterization of biomaterials and examination on cell models in vitro. We examined biomaterials intended for the regeneration of hard tissues, e.g. materials based on hydroxyapatite, calcium phosphate, of natural and synthetic origin, as well as biomaterials for the regeneration of soft tissues, e.g. materials based on collagen or other polymers of both natural and synthetic origin. In order to improve the biofunctionality of biomaterials, bioactive substances or their combinations are often added, and the biomaterials can be in different forms, in the form of scaffolds, in the form of granules, nano- or micro-dimensions, membranes and many others, whereby the shape of the material also affects the selection of the cell model used for testing. To characterize bimaterials, we used methods such as SEM, EDX, FTIR and others. In addition to cytotoxicity and genotoxicity tests, we performed proliferation, migration, differentiation, phagocytosis and many other tests, and we tested biomaterials both in direct contact with cells and in indirect contact. We also examined changes in biomaterials after interaction with cells, as well as the behavior of biomaterials in various conditions in vitro.",
publisher = "Banja Luka : ANURS",
journal = "Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.",
title = "Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације, Interaction of cells with different biomaterials in vitro and methods of characterization",
url = "https://hdl.handle.net/21.15107/rcub_dais_13654"
}

Stojanović, S., Najdanović, J., Mitić, Ž., Vasiljević, P., Ignjatović, N.,& Najman, S.. (2022). Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
Banja Luka : ANURS..
https://hdl.handle.net/21.15107/rcub_dais_13654

Stojanović S, Najdanović J, Mitić Ž, Vasiljević P, Ignjatović N, Najman S. Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.. 2022;.
https://hdl.handle.net/21.15107/rcub_dais_13654 .

Stojanović, Sanja, Najdanović, Jelena, Mitić, Žarko, Vasiljević, Perica, Ignjatović, Nenad, Najman, Stevo, "Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације" in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022. (2022),
https://hdl.handle.net/21.15107/rcub_dais_13654 .

TY  - CONF
AU  - Pantović-Pavlović, Marijana
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Pavlović, Miroslav M.
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13652
AB  - This paper deals with the deposition of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium with improved properties by in situ anodization/anaphoretic deposition process. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and post-treatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: FE-SEM, XRD, FTIR, cytotoxicity and in vivo testing.
PB  - Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM
C3  - Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia
T1  - Novel Calcium Phosphate Coatings with Selenum on Titanium
SP  - poster-102
UR  - https://hdl.handle.net/21.15107/rcub_dais_13652
ER  - 

@conference{
author = "Pantović-Pavlović, Marijana and Ignjatović, Nenad and Panić, Vladimir V. and Pavlović, Miroslav M.",
year = "2022",
abstract = "This paper deals with the deposition of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium with improved properties by in situ anodization/anaphoretic deposition process. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and post-treatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: FE-SEM, XRD, FTIR, cytotoxicity and in vivo testing.",
publisher = "Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM",
journal = "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia",
title = "Novel Calcium Phosphate Coatings with Selenum on Titanium",
pages = "poster-102",
url = "https://hdl.handle.net/21.15107/rcub_dais_13652"
}

Pantović-Pavlović, M., Ignjatović, N., Panić, V. V.,& Pavlović, M. M.. (2022). Novel Calcium Phosphate Coatings with Selenum on Titanium. in Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia
Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM., poster-102.
https://hdl.handle.net/21.15107/rcub_dais_13652

Pantović-Pavlović M, Ignjatović N, Panić VV, Pavlović MM. Novel Calcium Phosphate Coatings with Selenum on Titanium. in Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia. 2022;:poster-102.
https://hdl.handle.net/21.15107/rcub_dais_13652 .

Pantović-Pavlović, Marijana, Ignjatović, Nenad, Panić, Vladimir V., Pavlović, Miroslav M., "Novel Calcium Phosphate Coatings with Selenum on Titanium" in Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia (2022):poster-102,
https://hdl.handle.net/21.15107/rcub_dais_13652 .

TY  - CONF
AU  - Pantović-Pavlović, Marijana
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Pavlović, Miroslav M.
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13653
AB  - This paper deals with the issue of defining a new method of anodizing/anaphoretic deposition for the application of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium and anodized titanium coatings with improved properties. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and posttreatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. It primarily deals with the creation and optimization of a new in situ anodizing/anaphoretic deposition process for obtaining multifunctional composite biomaterials. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The aim of the research was to define new in situ anodizing/anaphoretic deposition process and adequate modification of process parameters for application of composite calcium phosphate coatings on titanium and its alloys, wherein innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: AFM, SEM, FE-SEM, roughness testing, XRD, FTIR, bioactivity, biocompatibility, cytotoxicity, antimicrobial activity and in vivo testing.
PB  - Split : Central and EasternEuropeanCommittee for Thermal Analysis and Calorimetry (CEEC-TAC)
C3  - Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1))
T1  - Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_dais_13653
ER  - 

@conference{
author = "Pantović-Pavlović, Marijana and Ignjatović, Nenad and Panić, Vladimir V. and Pavlović, Miroslav M.",
year = "2022",
abstract = "This paper deals with the issue of defining a new method of anodizing/anaphoretic deposition for the application of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium and anodized titanium coatings with improved properties. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and posttreatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. It primarily deals with the creation and optimization of a new in situ anodizing/anaphoretic deposition process for obtaining multifunctional composite biomaterials. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The aim of the research was to define new in situ anodizing/anaphoretic deposition process and adequate modification of process parameters for application of composite calcium phosphate coatings on titanium and its alloys, wherein innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: AFM, SEM, FE-SEM, roughness testing, XRD, FTIR, bioactivity, biocompatibility, cytotoxicity, antimicrobial activity and in vivo testing.",
publisher = "Split : Central and EasternEuropeanCommittee for Thermal Analysis and Calorimetry (CEEC-TAC)",
journal = "Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1))",
title = "Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_dais_13653"
}

Pantović-Pavlović, M., Ignjatović, N., Panić, V. V.,& Pavlović, M. M.. (2022). Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications. in Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1))
Split : Central and EasternEuropeanCommittee for Thermal Analysis and Calorimetry (CEEC-TAC)., 44-44.
https://hdl.handle.net/21.15107/rcub_dais_13653

Pantović-Pavlović M, Ignjatović N, Panić VV, Pavlović MM. Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications. in Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1)). 2022;:44-44.
https://hdl.handle.net/21.15107/rcub_dais_13653 .

Pantović-Pavlović, Marijana, Ignjatović, Nenad, Panić, Vladimir V., Pavlović, Miroslav M., "Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications" in Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1)) (2022):44-44,
https://hdl.handle.net/21.15107/rcub_dais_13653 .

TY  - JOUR
AU  - Uskoković, Vuk
AU  - Ignjatović, Nenad
AU  - Škapin, Srečo
AU  - Uskoković, Dragan
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13563
AB  - Hydroxyapatite (HAp) is the major component of all boney tissues in mammals. Because of this omnipresence in the living world, HAp possesses an exceptional biocompatibility. The downside of this omnipresence, however, comes in the form of its mild to moderate biological activities. One means of augmenting these activities involves the doping of HAp with foreign ions. Here, the first synthesis and characterization of HAp doped with germanium ions is being reported. Germanium was deliberately integrated into the crystal lattice of HAp in the form of germanate anions. Approximately two thirds of the germanate ions introduced into the hydrothermal solution got incorporated into the HAp lattice, yielding the approximate stoichiometry of Ca10-x(PO4)5.62+y(GeO3)0.38(OH)2-z. Germanates replaced the phosphates of stoichiometric HAp and induced the expansion of the HAp lattice both along the screw axis of the calcium ion hexagons and in the direction parallel to the basal plane. Simultaneously, the larger size and the triple valency of the germanate ion as compared to the smaller and trivalent phosphates prompted the bond distortion and charge compensation through defect formation, which reduced the crystallinity and increased the microstrain of the HAp lattice. Vibrational spectroscopic analyses corroborated these crystallographic effects by demonstrating the enhanced heterogeneity of the environments surrounding the active modes after germanate ions were incorporated into HAp. Conforming to La Châtelier's principle, this reduction of the crystallographic order increased the capacity of the material for integration of adventitious carbonates. However, the inclusion of germanate ions induced a partial shift of these carbonates to the hydroxyl channel sites, thus decreasing the ratio of the B-type carbonation to the A-type carbonation. Introduced into HAp, germanium acted as a superb regulator of the particle size and morphology, enhancing their fineness and uniformity. Inclusion of germanate ions also increased the electrophoretic mobility and hydrodynamic surface charge density of the particles by reducing their size and by inducing a more stochastic distribution of terminal ionic groups due to the bending of the crystal facets. Overall, the doping of HAp with germanate ions facilitated the production of narrowly dispersed nanorods with a moderately enhanced structural disorder and with a pronounced potential for the biomedical niche. © 2022 The Author(s)
T2  - Ceramics International
T1  - Germanium-doped hydroxyapatite: Synthesis and characterization of a new substituted apatite
SP  - 27693
EP  - 27702
VL  - 48
IS  - 19
DO  - 10.1016/j.ceramint.2022.06.068
UR  - https://hdl.handle.net/21.15107/rcub_dais_13563
ER  - 

@article{
author = "Uskoković, Vuk and Ignjatović, Nenad and Škapin, Srečo and Uskoković, Dragan",
year = "2022",
abstract = "Hydroxyapatite (HAp) is the major component of all boney tissues in mammals. Because of this omnipresence in the living world, HAp possesses an exceptional biocompatibility. The downside of this omnipresence, however, comes in the form of its mild to moderate biological activities. One means of augmenting these activities involves the doping of HAp with foreign ions. Here, the first synthesis and characterization of HAp doped with germanium ions is being reported. Germanium was deliberately integrated into the crystal lattice of HAp in the form of germanate anions. Approximately two thirds of the germanate ions introduced into the hydrothermal solution got incorporated into the HAp lattice, yielding the approximate stoichiometry of Ca10-x(PO4)5.62+y(GeO3)0.38(OH)2-z. Germanates replaced the phosphates of stoichiometric HAp and induced the expansion of the HAp lattice both along the screw axis of the calcium ion hexagons and in the direction parallel to the basal plane. Simultaneously, the larger size and the triple valency of the germanate ion as compared to the smaller and trivalent phosphates prompted the bond distortion and charge compensation through defect formation, which reduced the crystallinity and increased the microstrain of the HAp lattice. Vibrational spectroscopic analyses corroborated these crystallographic effects by demonstrating the enhanced heterogeneity of the environments surrounding the active modes after germanate ions were incorporated into HAp. Conforming to La Châtelier's principle, this reduction of the crystallographic order increased the capacity of the material for integration of adventitious carbonates. However, the inclusion of germanate ions induced a partial shift of these carbonates to the hydroxyl channel sites, thus decreasing the ratio of the B-type carbonation to the A-type carbonation. Introduced into HAp, germanium acted as a superb regulator of the particle size and morphology, enhancing their fineness and uniformity. Inclusion of germanate ions also increased the electrophoretic mobility and hydrodynamic surface charge density of the particles by reducing their size and by inducing a more stochastic distribution of terminal ionic groups due to the bending of the crystal facets. Overall, the doping of HAp with germanate ions facilitated the production of narrowly dispersed nanorods with a moderately enhanced structural disorder and with a pronounced potential for the biomedical niche. © 2022 The Author(s)",
journal = "Ceramics International",
title = "Germanium-doped hydroxyapatite: Synthesis and characterization of a new substituted apatite",
pages = "27693-27702",
volume = "48",
number = "19",
doi = "10.1016/j.ceramint.2022.06.068",
url = "https://hdl.handle.net/21.15107/rcub_dais_13563"
}

Uskoković, V., Ignjatović, N., Škapin, S.,& Uskoković, D.. (2022). Germanium-doped hydroxyapatite: Synthesis and characterization of a new substituted apatite. in Ceramics International, 48(19), 27693-27702.
https://doi.org/10.1016/j.ceramint.2022.06.068
https://hdl.handle.net/21.15107/rcub_dais_13563

Uskoković V, Ignjatović N, Škapin S, Uskoković D. Germanium-doped hydroxyapatite: Synthesis and characterization of a new substituted apatite. in Ceramics International. 2022;48(19):27693-27702.
doi:10.1016/j.ceramint.2022.06.068
https://hdl.handle.net/21.15107/rcub_dais_13563 .

Uskoković, Vuk, Ignjatović, Nenad, Škapin, Srečo, Uskoković, Dragan, "Germanium-doped hydroxyapatite: Synthesis and characterization of a new substituted apatite" in Ceramics International, 48, no. 19 (2022):27693-27702,
https://doi.org/10.1016/j.ceramint.2022.06.068 .,
https://hdl.handle.net/21.15107/rcub_dais_13563 .

TY  - JOUR
AU  - Kuzminac, Ivana
AU  - Ćelić, Andjelka S.
AU  - Bekić, Sofija S.
AU  - Kojić, Vesna
AU  - Savić, Marina P.
AU  - Ignjatović, Nenad
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13031
AB  - Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.
PB  - Elsevier BV
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
SP  - 112597
VL  - 216
DO  - 10.1016/j.colsurfb.2022.112597
UR  - https://hdl.handle.net/21.15107/rcub_dais_13031
ER  - 

@article{
author = "Kuzminac, Ivana and Ćelić, Andjelka S. and Bekić, Sofija S. and Kojić, Vesna and Savić, Marina P. and Ignjatović, Nenad",
year = "2022",
abstract = "Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.",
publisher = "Elsevier BV",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells",
pages = "112597",
volume = "216",
doi = "10.1016/j.colsurfb.2022.112597",
url = "https://hdl.handle.net/21.15107/rcub_dais_13031"
}

Kuzminac, I., Ćelić, A. S., Bekić, S. S., Kojić, V., Savić, M. P.,& Ignjatović, N.. (2022). Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces
Elsevier BV., 216, 112597.
https://doi.org/10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13031

Kuzminac I, Ćelić AS, Bekić SS, Kojić V, Savić MP, Ignjatović N. Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces. 2022;216:112597.
doi:10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13031 .

Kuzminac, Ivana, Ćelić, Andjelka S., Bekić, Sofija S., Kojić, Vesna, Savić, Marina P., Ignjatović, Nenad, "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells" in Colloids and Surfaces B: Biointerfaces, 216 (2022):112597,
https://doi.org/10.1016/j.colsurfb.2022.112597 .,
https://hdl.handle.net/21.15107/rcub_dais_13031 .

TY  - JOUR
AU  - Kuzminac, Ivana
AU  - Ćelić, Andjelka S.
AU  - Bekić, Sofija S.
AU  - Kojić, Vesna
AU  - Savić, Marina P.
AU  - Ignjatović, Nenad
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13030
AB  - Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.
PB  - Elsevier BV
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
SP  - 112597
VL  - 216
DO  - 10.1016/j.colsurfb.2022.112597
UR  - https://hdl.handle.net/21.15107/rcub_dais_13030
ER  - 

@article{
author = "Kuzminac, Ivana and Ćelić, Andjelka S. and Bekić, Sofija S. and Kojić, Vesna and Savić, Marina P. and Ignjatović, Nenad",
year = "2022",
abstract = "Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.",
publisher = "Elsevier BV",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells",
pages = "112597",
volume = "216",
doi = "10.1016/j.colsurfb.2022.112597",
url = "https://hdl.handle.net/21.15107/rcub_dais_13030"
}

Kuzminac, I., Ćelić, A. S., Bekić, S. S., Kojić, V., Savić, M. P.,& Ignjatović, N.. (2022). Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces
Elsevier BV., 216, 112597.
https://doi.org/10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13030

Kuzminac I, Ćelić AS, Bekić SS, Kojić V, Savić MP, Ignjatović N. Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces. 2022;216:112597.
doi:10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13030 .

Kuzminac, Ivana, Ćelić, Andjelka S., Bekić, Sofija S., Kojić, Vesna, Savić, Marina P., Ignjatović, Nenad, "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells" in Colloids and Surfaces B: Biointerfaces, 216 (2022):112597,
https://doi.org/10.1016/j.colsurfb.2022.112597 .,
https://hdl.handle.net/21.15107/rcub_dais_13030 .

TY  - JOUR
AU  - Degli Esposti, Lorenzo
AU  - Marković, Smilja
AU  - Ignjatović, Nenad
AU  - Panseri, Silvia
AU  - Montesi, Monica
AU  - Adamiano, Alessio
AU  - Fosca, Marco
AU  - Rau, Julietta V.
AU  - Uskoković, Vuk
AU  - Iafisco, Michele
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11639
AB  - Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics
SP  - 4832
EP  - 4845
VL  - 9
IS  - 24
DO  - 10.1039/D1TB00601K
UR  - https://hdl.handle.net/21.15107/rcub_dais_11639
ER  - 

@article{
author = "Degli Esposti, Lorenzo and Marković, Smilja and Ignjatović, Nenad and Panseri, Silvia and Montesi, Monica and Adamiano, Alessio and Fosca, Marco and Rau, Julietta V. and Uskoković, Vuk and Iafisco, Michele",
year = "2021",
abstract = "Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics",
pages = "4832-4845",
volume = "9",
number = "24",
doi = "10.1039/D1TB00601K",
url = "https://hdl.handle.net/21.15107/rcub_dais_11639"
}

Degli Esposti, L., Marković, S., Ignjatović, N., Panseri, S., Montesi, M., Adamiano, A., Fosca, M., Rau, J. V., Uskoković, V.,& Iafisco, M.. (2021). Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B
Royal Society of Chemistry., 9(24), 4832-4845.
https://doi.org/10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11639

Degli Esposti L, Marković S, Ignjatović N, Panseri S, Montesi M, Adamiano A, Fosca M, Rau JV, Uskoković V, Iafisco M. Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B. 2021;9(24):4832-4845.
doi:10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11639 .

Degli Esposti, Lorenzo, Marković, Smilja, Ignjatović, Nenad, Panseri, Silvia, Montesi, Monica, Adamiano, Alessio, Fosca, Marco, Rau, Julietta V., Uskoković, Vuk, Iafisco, Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics" in Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845,
https://doi.org/10.1039/D1TB00601K .,
https://hdl.handle.net/21.15107/rcub_dais_11639 .

TY  - JOUR
AU  - Degli Esposti, Lorenzo
AU  - Marković, Smilja
AU  - Ignjatović, Nenad
AU  - Panseri, Silvia
AU  - Montesi, Monica
AU  - Adamiano, Alessio
AU  - Fosca, Marco
AU  - Rau, Julietta V.
AU  - Uskoković, Vuk
AU  - Iafisco, Michele
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11640
AB  - Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics
SP  - 4832
EP  - 4845
VL  - 9
IS  - 24
DO  - 10.1039/D1TB00601K
UR  - https://hdl.handle.net/21.15107/rcub_dais_11640
ER  - 

@article{
author = "Degli Esposti, Lorenzo and Marković, Smilja and Ignjatović, Nenad and Panseri, Silvia and Montesi, Monica and Adamiano, Alessio and Fosca, Marco and Rau, Julietta V. and Uskoković, Vuk and Iafisco, Michele",
year = "2021",
abstract = "Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics",
pages = "4832-4845",
volume = "9",
number = "24",
doi = "10.1039/D1TB00601K",
url = "https://hdl.handle.net/21.15107/rcub_dais_11640"
}

Degli Esposti, L., Marković, S., Ignjatović, N., Panseri, S., Montesi, M., Adamiano, A., Fosca, M., Rau, J. V., Uskoković, V.,& Iafisco, M.. (2021). Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B
Royal Society of Chemistry., 9(24), 4832-4845.
https://doi.org/10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11640

Degli Esposti L, Marković S, Ignjatović N, Panseri S, Montesi M, Adamiano A, Fosca M, Rau JV, Uskoković V, Iafisco M. Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B. 2021;9(24):4832-4845.
doi:10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11640 .

Degli Esposti, Lorenzo, Marković, Smilja, Ignjatović, Nenad, Panseri, Silvia, Montesi, Monica, Adamiano, Alessio, Fosca, Marco, Rau, Julietta V., Uskoković, Vuk, Iafisco, Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics" in Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845,
https://doi.org/10.1039/D1TB00601K .,
https://hdl.handle.net/21.15107/rcub_dais_11640 .

TY  - DATA
AU  - Degli Esposti, Lorenzo
AU  - Marković, Smilja
AU  - Ignjatović, Nenad
AU  - Panseri, Silvia
AU  - Montesi, Monica
AU  - Adamiano, Alessio
AU  - Fosca, Marco
AU  - Rau, Julietta V.
AU  - Uskoković, Vuk
AU  - Iafisco, Michele
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11642
AB  - Figure S1. The shift in the crystallization peak for (A) Cit-ACP-1 and (B) Cit-FACP-1 to higher temperatures in direct proportion with the heating rate; Figure S2. TGA curves of calcined Cit-ACP-4, Cit-ACP-2, and Cit-ACP-1; Figure S3. Pictures of calcined (A) Cit-ACP-4 and (B) Cit-ACP-1
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K
VL  - 9
IS  - 24
UR  - https://hdl.handle.net/21.15107/rcub_dais_11642
ER  - 

@misc{
author = "Degli Esposti, Lorenzo and Marković, Smilja and Ignjatović, Nenad and Panseri, Silvia and Montesi, Monica and Adamiano, Alessio and Fosca, Marco and Rau, Julietta V. and Uskoković, Vuk and Iafisco, Michele",
year = "2021",
abstract = "Figure S1. The shift in the crystallization peak for (A) Cit-ACP-1 and (B) Cit-FACP-1 to higher temperatures in direct proportion with the heating rate; Figure S2. TGA curves of calcined Cit-ACP-4, Cit-ACP-2, and Cit-ACP-1; Figure S3. Pictures of calcined (A) Cit-ACP-4 and (B) Cit-ACP-1",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K",
volume = "9",
number = "24",
url = "https://hdl.handle.net/21.15107/rcub_dais_11642"
}

Degli Esposti, L., Marković, S., Ignjatović, N., Panseri, S., Montesi, M., Adamiano, A., Fosca, M., Rau, J. V., Uskoković, V.,& Iafisco, M.. (2021). Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K. in Journal of Materials Chemistry B
Royal Society of Chemistry., 9(24).
https://hdl.handle.net/21.15107/rcub_dais_11642

Degli Esposti L, Marković S, Ignjatović N, Panseri S, Montesi M, Adamiano A, Fosca M, Rau JV, Uskoković V, Iafisco M. Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K. in Journal of Materials Chemistry B. 2021;9(24).
https://hdl.handle.net/21.15107/rcub_dais_11642 .

Degli Esposti, Lorenzo, Marković, Smilja, Ignjatović, Nenad, Panseri, Silvia, Montesi, Monica, Adamiano, Alessio, Fosca, Marco, Rau, Julietta V., Uskoković, Vuk, Iafisco, Michele, "Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K" in Journal of Materials Chemistry B, 9, no. 24 (2021),
https://hdl.handle.net/21.15107/rcub_dais_11642 .