TY  - JOUR
AU  - Lazarević, Miloš
AU  - Petrović, Sanja
AU  - Pierfelice, Tania Vanessa
AU  - Ignjatović, Nenad
AU  - Piattelli, Adriano
AU  - Vlajić Tovilović, Tamara
AU  - Radunović, Milena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14287
AB  - Collagen membranes are routinely used in oral surgery for bone regeneration. Despite their numerous advantages, such as stimulating bone growth, bacterial contamination still remains one of the disadvantages of membrane use. Thus, we assessed the biocompatibility and osteogenic and antibacterial properties of a collagen membrane (OsteoBiol) modified with chitosan (CHI) and hydroxyapatite nanoparticles (HApNPs). Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FT-IR), X-ray powder diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) were performed for membrane characterization. Biocompatibility was assessed on dental pulp stem cells (DPSCs) by an MTT assay, while the osteogenic effect was assessed by an ALP activity assay and qPCR analysis of osteogenic markers (BMP4, ALP, RUNX2, and OCN). Antimicrobial properties were investigated by counting colony-forming units (CFUs) of Streptococcus mitis, Porphyromonas gingivalis, and Fusobaterium nucleatum on membranes and in the surrounding medium. Membranes showed no cytotoxicity. ALP activity was higher and ALP, BMP4, and OCN genes were up-regulated in DPSCs on modified membranes compared to unmodified membranes. The CFUs were reduced on modified membranes and in the medium. Modified membranes showed great biocompatibility and a high osteoinductive effect. Additionally, they showed antimicrobial and antibiofilm effects against periopathogens. It can be concluded that the incorporation of CHI and hydroxyapatite nanoparticles in collagen membranes may be advantageous to promote osteogenesis and reduce bacterial adhesion.
PB  - Basel : MDPI AG
T2  - Biomolecules
T1  - Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite
SP  - 579
VL  - 13
IS  - 4
DO  - 10.3390/biom13040579
UR  - https://hdl.handle.net/21.15107/rcub_dais_14287
ER  - 

@article{
author = "Lazarević, Miloš and Petrović, Sanja and Pierfelice, Tania Vanessa and Ignjatović, Nenad and Piattelli, Adriano and Vlajić Tovilović, Tamara and Radunović, Milena",
year = "2023",
abstract = "Collagen membranes are routinely used in oral surgery for bone regeneration. Despite their numerous advantages, such as stimulating bone growth, bacterial contamination still remains one of the disadvantages of membrane use. Thus, we assessed the biocompatibility and osteogenic and antibacterial properties of a collagen membrane (OsteoBiol) modified with chitosan (CHI) and hydroxyapatite nanoparticles (HApNPs). Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FT-IR), X-ray powder diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) were performed for membrane characterization. Biocompatibility was assessed on dental pulp stem cells (DPSCs) by an MTT assay, while the osteogenic effect was assessed by an ALP activity assay and qPCR analysis of osteogenic markers (BMP4, ALP, RUNX2, and OCN). Antimicrobial properties were investigated by counting colony-forming units (CFUs) of Streptococcus mitis, Porphyromonas gingivalis, and Fusobaterium nucleatum on membranes and in the surrounding medium. Membranes showed no cytotoxicity. ALP activity was higher and ALP, BMP4, and OCN genes were up-regulated in DPSCs on modified membranes compared to unmodified membranes. The CFUs were reduced on modified membranes and in the medium. Modified membranes showed great biocompatibility and a high osteoinductive effect. Additionally, they showed antimicrobial and antibiofilm effects against periopathogens. It can be concluded that the incorporation of CHI and hydroxyapatite nanoparticles in collagen membranes may be advantageous to promote osteogenesis and reduce bacterial adhesion.",
publisher = "Basel : MDPI AG",
journal = "Biomolecules",
title = "Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite",
pages = "579",
volume = "13",
number = "4",
doi = "10.3390/biom13040579",
url = "https://hdl.handle.net/21.15107/rcub_dais_14287"
}

Lazarević, M., Petrović, S., Pierfelice, T. V., Ignjatović, N., Piattelli, A., Vlajić Tovilović, T.,& Radunović, M.. (2023). Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite. in Biomolecules
Basel : MDPI AG., 13(4), 579.
https://doi.org/10.3390/biom13040579
https://hdl.handle.net/21.15107/rcub_dais_14287

Lazarević M, Petrović S, Pierfelice TV, Ignjatović N, Piattelli A, Vlajić Tovilović T, Radunović M. Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite. in Biomolecules. 2023;13(4):579.
doi:10.3390/biom13040579
https://hdl.handle.net/21.15107/rcub_dais_14287 .

Lazarević, Miloš, Petrović, Sanja, Pierfelice, Tania Vanessa, Ignjatović, Nenad, Piattelli, Adriano, Vlajić Tovilović, Tamara, Radunović, Milena, "Antimicrobial and Osteogenic Effects of Collagen Membrane Decorated with Chitosan–Nano-Hydroxyapatite" in Biomolecules, 13, no. 4 (2023):579,
https://doi.org/10.3390/biom13040579 .,
https://hdl.handle.net/21.15107/rcub_dais_14287 .