Filipović, Nenad

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Authority KeyName Variants
orcid::0000-0003-2261-8066
  • Filipović, Nenad (39)
Projects
Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them Italian Ministry of Foreign Affairs and International Cooperation (MAECI) within the collaboration framework between Italy and the Republic of Serbia (project PGR02952, call “Grande Rilevanza”)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200175 (Institute of Technical Sciences of SASA, Belgrade) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200116 (University of Belgrade, Faculty of Agriculture)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200125 (University of Novi Sad, Faculty of Science) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200135 (University of Belgrade, Faculty of Technology and Metallurgy)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) Matériaux hiérarchiques et fonctionnels pour la santé, l’environnement et l’énergie (HiFunMat)
Bilateral collaboration between Serbia and Slovenia (BI-RS/16-17-039) European Science Foundation COST Action CA15114
Microbial diversity study and characterization of beneficial environmental microorganisms Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden
Slovenian Research Agency: Program P1-02456 Functional, Functionalized and Advanced Nanomaterials
Italian Ministry of University and Education (PRIN-2010 n. 2010B5B2NL) Ministry of Foreign Affairs and International Cooperation (Research Project of Particular Relevance between Italy and Serbia—PGR02952)
Serbian-German bilateral project no 451-03-01858/20 13-09/2 (DAAD project-ID 57060741) German Academic Exchange Service (DAAD project number 57514776)
Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan Examination of mechanisms of action, toxicity and interactions of adjuvant analgesics
Italian Ministry of University and Education, PRIN-2010 n. 2010B5B2NL

Author's Bibliography

2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior

Ristić, Predrag; Filipović, Nenad; Blagojević, Vladimir A.; Ćirković, Jovana; Holló, Berta Barta; Đokić, Veljko R.; Donnard, Morgan; Gulea, Mihaela; Marjanović, Ivana; Klisurić, Olivera R.; Todorović, Tamara R.

(Royal Society of Chemistry (RSC), 2021)

TY  - JOUR
AU  - Ristić, Predrag
AU  - Filipović, Nenad
AU  - Blagojević, Vladimir A.
AU  - Ćirković, Jovana
AU  - Holló, Berta Barta
AU  - Đokić, Veljko R.
AU  - Donnard, Morgan
AU  - Gulea, Mihaela
AU  - Marjanović, Ivana
AU  - Klisurić, Olivera R.
AU  - Todorović, Tamara R.
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11757
AB  - Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min−1. The best result was obtained for compound 4, which can be correlated to its largest lattice volume.
PB  - Royal Society of Chemistry (RSC)
T2  - CrystEngComm
T1  - 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior
SP  - 4799
EP  - 4815
VL  - 23
IS  - 27
DO  - 10.1039/D1CE00394A
UR  - https://hdl.handle.net/21.15107/rcub_dais_11757
ER  - 
@article{
author = "Ristić, Predrag and Filipović, Nenad and Blagojević, Vladimir A. and Ćirković, Jovana and Holló, Berta Barta and Đokić, Veljko R. and Donnard, Morgan and Gulea, Mihaela and Marjanović, Ivana and Klisurić, Olivera R. and Todorović, Tamara R.",
year = "2021",
abstract = "Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min−1. The best result was obtained for compound 4, which can be correlated to its largest lattice volume.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "CrystEngComm",
title = "2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior",
pages = "4799-4815",
volume = "23",
number = "27",
doi = "10.1039/D1CE00394A",
url = "https://hdl.handle.net/21.15107/rcub_dais_11757"
}
Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R.. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. in CrystEngComm
Royal Society of Chemistry (RSC)., 23(27), 4799-4815.
https://doi.org/10.1039/D1CE00394A
https://hdl.handle.net/21.15107/rcub_dais_11757
Ristić P, Filipović N, Blagojević VA, Ćirković J, Holló BB, Đokić VR, Donnard M, Gulea M, Marjanović I, Klisurić OR, Todorović TR. 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. in CrystEngComm. 2021;23(27):4799-4815.
doi:10.1039/D1CE00394A
https://hdl.handle.net/21.15107/rcub_dais_11757 .
Ristić, Predrag, Filipović, Nenad, Blagojević, Vladimir A., Ćirković, Jovana, Holló, Berta Barta, Đokić, Veljko R., Donnard, Morgan, Gulea, Mihaela, Marjanović, Ivana, Klisurić, Olivera R., Todorović, Tamara R., "2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior" in CrystEngComm, 23, no. 27 (2021):4799-4815,
https://doi.org/10.1039/D1CE00394A .,
https://hdl.handle.net/21.15107/rcub_dais_11757 .
4

2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior

Ristić, Predrag; Filipović, Nenad; Blagojević, Vladimir A.; Ćirković, Jovana; Holló, Berta Barta; Đokić, Veljko R.; Donnard, Morgan; Gulea, Mihaela; Marjanović, Ivana; Klisurić, Olivera R.; Todorović, Tamara R.

(Royal Society of Chemistry (RSC), 2021)

TY  - JOUR
AU  - Ristić, Predrag
AU  - Filipović, Nenad
AU  - Blagojević, Vladimir A.
AU  - Ćirković, Jovana
AU  - Holló, Berta Barta
AU  - Đokić, Veljko R.
AU  - Donnard, Morgan
AU  - Gulea, Mihaela
AU  - Marjanović, Ivana
AU  - Klisurić, Olivera R.
AU  - Todorović, Tamara R.
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11753
AB  - Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min−1. The best result was obtained for compound 4, which can be correlated to its largest lattice volume.
PB  - Royal Society of Chemistry (RSC)
T2  - CrystEngComm
T1  - 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior
SP  - 4799
EP  - 4815
VL  - 23
IS  - 27
DO  - 10.1039/D1CE00394A
UR  - https://hdl.handle.net/21.15107/rcub_dais_11753
ER  - 
@article{
author = "Ristić, Predrag and Filipović, Nenad and Blagojević, Vladimir A. and Ćirković, Jovana and Holló, Berta Barta and Đokić, Veljko R. and Donnard, Morgan and Gulea, Mihaela and Marjanović, Ivana and Klisurić, Olivera R. and Todorović, Tamara R.",
year = "2021",
abstract = "Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min−1. The best result was obtained for compound 4, which can be correlated to its largest lattice volume.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "CrystEngComm",
title = "2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior",
pages = "4799-4815",
volume = "23",
number = "27",
doi = "10.1039/D1CE00394A",
url = "https://hdl.handle.net/21.15107/rcub_dais_11753"
}
Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R.. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. in CrystEngComm
Royal Society of Chemistry (RSC)., 23(27), 4799-4815.
https://doi.org/10.1039/D1CE00394A
https://hdl.handle.net/21.15107/rcub_dais_11753
Ristić P, Filipović N, Blagojević VA, Ćirković J, Holló BB, Đokić VR, Donnard M, Gulea M, Marjanović I, Klisurić OR, Todorović TR. 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. in CrystEngComm. 2021;23(27):4799-4815.
doi:10.1039/D1CE00394A
https://hdl.handle.net/21.15107/rcub_dais_11753 .
Ristić, Predrag, Filipović, Nenad, Blagojević, Vladimir A., Ćirković, Jovana, Holló, Berta Barta, Đokić, Veljko R., Donnard, Morgan, Gulea, Mihaela, Marjanović, Ivana, Klisurić, Olivera R., Todorović, Tamara R., "2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior" in CrystEngComm, 23, no. 27 (2021):4799-4815,
https://doi.org/10.1039/D1CE00394A .,
https://hdl.handle.net/21.15107/rcub_dais_11753 .
4

Supporting information for Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786

Dinić, Miroslav; Novović, Katarina; Ivković, Branka; Filipović, Nenad; Stevanović, Magdalena; Milenković, Marina

(Wiley-VCH Verlag, 2021)

TY  - DATA
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11879
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley-VCH Verlag
T2  - Chemistry and Biodiversity
T1  - Supporting information for Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786
VL  - 18
IS  - 1
UR  - https://hdl.handle.net/21.15107/rcub_dais_11879
ER  - 
@misc{
author = "Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2021",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley-VCH Verlag",
journal = "Chemistry and Biodiversity",
title = "Supporting information for Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786",
volume = "18",
number = "1",
url = "https://hdl.handle.net/21.15107/rcub_dais_11879"
}
Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2021). Supporting information for Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786. in Chemistry and Biodiversity
Wiley-VCH Verlag., 18(1).
https://hdl.handle.net/21.15107/rcub_dais_11879
Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Supporting information for Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786. in Chemistry and Biodiversity. 2021;18(1).
https://hdl.handle.net/21.15107/rcub_dais_11879 .
Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Supporting information for Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786, https://doi.org/10.1002/cbdv.202000786" in Chemistry and Biodiversity, 18, no. 1 (2021),
https://hdl.handle.net/21.15107/rcub_dais_11879 .

Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,Adhesion and Surface Motility of Acinetobacter baumannii byInhibiting ompA Gene Expression

Ušjak, Dušan; Dinić, Miroslav; Novović, Katarina; Ivković, Branka; Filipović, Nenad; Stevanović, Magdalena; Milenković, Marina

(Wiley-VCH, 2021)

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11878
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley-VCH
T2  - Chemistry and Biodiversity
T1  - Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,Adhesion and Surface Motility of Acinetobacter baumannii byInhibiting ompA Gene Expression
SP  - e2000786
VL  - 18
IS  - 1
DO  - 10.1002/cbdv.202000786
UR  - https://hdl.handle.net/21.15107/rcub_dais_11878
ER  - 
@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2021",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley-VCH",
journal = "Chemistry and Biodiversity",
title = "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,Adhesion and Surface Motility of Acinetobacter baumannii byInhibiting ompA Gene Expression",
pages = "e2000786",
volume = "18",
number = "1",
doi = "10.1002/cbdv.202000786",
url = "https://hdl.handle.net/21.15107/rcub_dais_11878"
}
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2021). Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,Adhesion and Surface Motility of Acinetobacter baumannii byInhibiting ompA Gene Expression. in Chemistry and Biodiversity
Wiley-VCH., 18(1), e2000786.
https://doi.org/10.1002/cbdv.202000786
https://hdl.handle.net/21.15107/rcub_dais_11878
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,Adhesion and Surface Motility of Acinetobacter baumannii byInhibiting ompA Gene Expression. in Chemistry and Biodiversity. 2021;18(1):e2000786.
doi:10.1002/cbdv.202000786
https://hdl.handle.net/21.15107/rcub_dais_11878 .
Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,Adhesion and Surface Motility of Acinetobacter baumannii byInhibiting ompA Gene Expression" in Chemistry and Biodiversity, 18, no. 1 (2021):e2000786,
https://doi.org/10.1002/cbdv.202000786 .,
https://hdl.handle.net/21.15107/rcub_dais_11878 .
2
1
1

Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression

Ušjak, Dušan; Dinić, Miroslav; Novović, Katarina; Ivković, Branka; Filipović, Nenad; Stevanović, Magdalena; Milenković, Marina T.

(Wiley, 2021)

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina T.
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/10034
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley
T2  - Chemistry & Biodiversity
T1  - Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression
SP  - e2000786
VL  - 18
IS  - 1
DO  - 10.1002/cbdv.202000786
UR  - https://hdl.handle.net/21.15107/rcub_dais_10034
ER  - 
@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.",
year = "2021",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity",
title = "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression",
pages = "e2000786",
volume = "18",
number = "1",
doi = "10.1002/cbdv.202000786",
url = "https://hdl.handle.net/21.15107/rcub_dais_10034"
}
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2021). Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression. in Chemistry & Biodiversity
Wiley., 18(1), e2000786.
https://doi.org/10.1002/cbdv.202000786
https://hdl.handle.net/21.15107/rcub_dais_10034
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression. in Chemistry & Biodiversity. 2021;18(1):e2000786.
doi:10.1002/cbdv.202000786
https://hdl.handle.net/21.15107/rcub_dais_10034 .
Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021):e2000786,
https://doi.org/10.1002/cbdv.202000786 .,
https://hdl.handle.net/21.15107/rcub_dais_10034 .
2
1
1

Crystallographic Information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A

Ristić, Predrag; Filipović, Nenad; Blagojević, Vladimir A.; Ćirković, Jovana; Holló, Berta Barta; Đokić, Veljko R.; Donnard, Morgan; Gulea, Mihaela; Marjanović, Ivana; Klisurić, Olivera R.; Todorović, Tamara R.

(Royal Society of Chemistry (RSC), 2021)

TY  - DATA
AU  - Ristić, Predrag
AU  - Filipović, Nenad
AU  - Blagojević, Vladimir A.
AU  - Ćirković, Jovana
AU  - Holló, Berta Barta
AU  - Đokić, Veljko R.
AU  - Donnard, Morgan
AU  - Gulea, Mihaela
AU  - Marjanović, Ivana
AU  - Klisurić, Olivera R.
AU  - Todorović, Tamara R.
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11761
AB  - Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min−1. The best result was obtained for compound 4, which can be correlated to its largest lattice volume.
PB  - Royal Society of Chemistry (RSC)
T2  - CrystEngComm
T1  - Crystallographic Information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A
VL  - 23
IS  - 27
UR  - https://hdl.handle.net/21.15107/rcub_dais_11761
ER  - 
@misc{
author = "Ristić, Predrag and Filipović, Nenad and Blagojević, Vladimir A. and Ćirković, Jovana and Holló, Berta Barta and Đokić, Veljko R. and Donnard, Morgan and Gulea, Mihaela and Marjanović, Ivana and Klisurić, Olivera R. and Todorović, Tamara R.",
year = "2021",
abstract = "Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. Topological analysis in standard representations suggests that underlying nets in 1 and 2 have an sql topology, while 3 and 4 exhibit a dia topology. Thermal analysis shows that 3 loses crystalline water at room temperature, while other compounds show good thermal stability. All compounds show good photocatalytic activity for photocatalytic degradation of the mordant blue 9 dye, with reaction rates in the range 0.029 to 0.061 min−1. The best result was obtained for compound 4, which can be correlated to its largest lattice volume.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "CrystEngComm",
title = "Crystallographic Information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A",
volume = "23",
number = "27",
url = "https://hdl.handle.net/21.15107/rcub_dais_11761"
}
Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R.. (2021). Crystallographic Information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A. in CrystEngComm
Royal Society of Chemistry (RSC)., 23(27).
https://hdl.handle.net/21.15107/rcub_dais_11761
Ristić P, Filipović N, Blagojević VA, Ćirković J, Holló BB, Đokić VR, Donnard M, Gulea M, Marjanović I, Klisurić OR, Todorović TR. Crystallographic Information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A. in CrystEngComm. 2021;23(27).
https://hdl.handle.net/21.15107/rcub_dais_11761 .
Ristić, Predrag, Filipović, Nenad, Blagojević, Vladimir A., Ćirković, Jovana, Holló, Berta Barta, Đokić, Veljko R., Donnard, Morgan, Gulea, Mihaela, Marjanović, Ivana, Klisurić, Olivera R., Todorović, Tamara R., "Crystallographic Information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A" in CrystEngComm, 23, no. 27 (2021),
https://hdl.handle.net/21.15107/rcub_dais_11761 .

Supplementary information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A

Ristić, Predrag; Filipović, Nenad; Blagojević, Vladimir A.; Ćirković, Jovana; Holló, Berta Barta; Đokić, Veljko R.; Donnard, Morgan; Gulea, Mihaela; Marjanović, Ivana; Klisurić, Olivera R.; Todorović, Tamara R.

(Royal Society of Chemistry (RSC), 2021)

TY  - DATA
AU  - Ristić, Predrag
AU  - Filipović, Nenad
AU  - Blagojević, Vladimir A.
AU  - Ćirković, Jovana
AU  - Holló, Berta Barta
AU  - Đokić, Veljko R.
AU  - Donnard, Morgan
AU  - Gulea, Mihaela
AU  - Marjanović, Ivana
AU  - Klisurić, Olivera R.
AU  - Todorović, Tamara R.
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11759
AB  - Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. The supplementary ifnormation contains: Selected hydrogen-bond parameters for 3, solid-state optical absorption spectra for crystalline samples of 1–4, plots of concentration ratios against irradiation time for 1–4 and Degussa P25, the changes in photodegradation percentage of MB9 after three successive cycles for 4, IR spectra of pristine and CP 4 after the third cycle and UV–vis spectra for the MB9 solution in the presence of 4 and quenchers. CCDC numbers are 2068533–2068536 for compounds 1–4, respectively.
PB  - Royal Society of Chemistry (RSC)
T2  - CrystEngComm
T1  - Supplementary information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A
VL  - 23
IS  - 27
UR  - https://hdl.handle.net/21.15107/rcub_dais_11759
ER  - 
@misc{
author = "Ristić, Predrag and Filipović, Nenad and Blagojević, Vladimir A. and Ćirković, Jovana and Holló, Berta Barta and Đokić, Veljko R. and Donnard, Morgan and Gulea, Mihaela and Marjanović, Ivana and Klisurić, Olivera R. and Todorović, Tamara R.",
year = "2021",
abstract = "Four silver-based coordination polymers, {[Ag(L1)2]NO3}∞ (1), {[Ag(L1)2]ClO4}∞ (2), {[Ag(L2)2]NO3·H2O}∞ (3) and {[Ag(L2)2]ClO4}∞ (4), were synthesized using the thiomorpholine-4-carbonitrile (L1) and piperazine-1,4-dicarbonitrile (L2) ligands. Compounds 1 and 2 are two-dimensional, while 3 and 4 are three-dimensional. L1 and L2 are 1,4-bis-monodentate ligands in all compounds, while Ag(I) ions are four-coordinated in a slightly distorted tetrahedral geometry. The supplementary ifnormation contains: Selected hydrogen-bond parameters for 3, solid-state optical absorption spectra for crystalline samples of 1–4, plots of concentration ratios against irradiation time for 1–4 and Degussa P25, the changes in photodegradation percentage of MB9 after three successive cycles for 4, IR spectra of pristine and CP 4 after the third cycle and UV–vis spectra for the MB9 solution in the presence of 4 and quenchers. CCDC numbers are 2068533–2068536 for compounds 1–4, respectively.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "CrystEngComm",
title = "Supplementary information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A",
volume = "23",
number = "27",
url = "https://hdl.handle.net/21.15107/rcub_dais_11759"
}
Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R.. (2021). Supplementary information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A. in CrystEngComm
Royal Society of Chemistry (RSC)., 23(27).
https://hdl.handle.net/21.15107/rcub_dais_11759
Ristić P, Filipović N, Blagojević VA, Ćirković J, Holló BB, Đokić VR, Donnard M, Gulea M, Marjanović I, Klisurić OR, Todorović TR. Supplementary information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A. in CrystEngComm. 2021;23(27).
https://hdl.handle.net/21.15107/rcub_dais_11759 .
Ristić, Predrag, Filipović, Nenad, Blagojević, Vladimir A., Ćirković, Jovana, Holló, Berta Barta, Đokić, Veljko R., Donnard, Morgan, Gulea, Mihaela, Marjanović, Ivana, Klisurić, Olivera R., Todorović, Tamara R., "Supplementary information for the article: Ristić, P., Filipović, N., Blagojević, V. A., Ćirković, J., Holló, B. B., Đokić, V. R., Donnard, M., Gulea, M., Marjanović, I., Klisurić, O. R.,& Todorović, T. R. (2021). 2D and 3D silver-based coordination polymers with thiomorpholine-4-carbonitrile and piperazine-1,4-dicarbonitrile: structure, intermolecular interactions, photocatalysis, and thermal behavior. CrystEngComm, Royal Society of Chemistry (RSC), 23(27), 4799-4815. https://doi.org/10.1039/D1CE00394A" in CrystEngComm, 23, no. 27 (2021),
https://hdl.handle.net/21.15107/rcub_dais_11759 .

Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure

Filipović, Nenad; Ušjak, Dušan; Milenković, Marina; Zheng, Kai; Liverani, Liliana; Boccaccini, Aldo; Stevanović, Magdalena

(Frontiers Media SA, 2021)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Liverani, Liliana
AU  - Boccaccini, Aldo
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11631
AB  - Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.
PB  - Frontiers Media SA
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure
VL  - 8
DO  - 10.3389/fbioe.2020.624621
UR  - https://hdl.handle.net/21.15107/rcub_dais_11631
ER  - 
@article{
author = "Filipović, Nenad and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Liverani, Liliana and Boccaccini, Aldo and Stevanović, Magdalena",
year = "2021",
abstract = "Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure",
volume = "8",
doi = "10.3389/fbioe.2020.624621",
url = "https://hdl.handle.net/21.15107/rcub_dais_11631"
}
Filipović, N., Ušjak, D., Milenković, M., Zheng, K., Liverani, L., Boccaccini, A.,& Stevanović, M.. (2021). Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology
Frontiers Media SA., 8.
https://doi.org/10.3389/fbioe.2020.624621
https://hdl.handle.net/21.15107/rcub_dais_11631
Filipović N, Ušjak D, Milenković M, Zheng K, Liverani L, Boccaccini A, Stevanović M. Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology. 2021;8.
doi:10.3389/fbioe.2020.624621
https://hdl.handle.net/21.15107/rcub_dais_11631 .
Filipović, Nenad, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Liverani, Liliana, Boccaccini, Aldo, Stevanović, Magdalena, "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure" in Frontiers in Bioengineering and Biotechnology, 8 (2021),
https://doi.org/10.3389/fbioe.2020.624621 .,
https://hdl.handle.net/21.15107/rcub_dais_11631 .
3
8
4
7

Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression

Ušjak, Dušan; Dinić, Miroslav; Novović, Katarina; Ivković, Branka; Filipović, Nenad; Stevanović, Magdalena; Milenković, Marina

(2020)

TY  - CONF
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3758
UR  - https://dais.sanu.ac.rs/123456789/10086
AB  - Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena.
AB  - Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.
C3  - FEMS Online Conference on Microbiology
T1  - Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression
UR  - https://hdl.handle.net/21.15107/rcub_dais_10086
ER  - 
@conference{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2020",
abstract = "Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena., Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.",
journal = "FEMS Online Conference on Microbiology",
title = "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression",
url = "https://hdl.handle.net/21.15107/rcub_dais_10086"
}
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2020). Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. in FEMS Online Conference on Microbiology.
https://hdl.handle.net/21.15107/rcub_dais_10086
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. in FEMS Online Conference on Microbiology. 2020;.
https://hdl.handle.net/21.15107/rcub_dais_10086 .
Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression" in FEMS Online Conference on Microbiology (2020),
https://hdl.handle.net/21.15107/rcub_dais_10086 .

Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(2019)

TY  - DATA
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/5972
T2  - Materials Science and Engineering C
T1  - Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073
UR  - https://hdl.handle.net/21.15107/rcub_dais_5972
ER  - 
@misc{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
journal = "Materials Science and Engineering C",
title = "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073",
url = "https://hdl.handle.net/21.15107/rcub_dais_5972"
}
Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073. in Materials Science and Engineering C.
https://hdl.handle.net/21.15107/rcub_dais_5972
Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073. in Materials Science and Engineering C. 2019;.
https://hdl.handle.net/21.15107/rcub_dais_5972 .
Filipović, Nenad, Veselinović, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipič, Metka, Žegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073" in Materials Science and Engineering C (2019),
https://hdl.handle.net/21.15107/rcub_dais_5972 .

Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(Elsevier, 2019)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/4600
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
SP  - 776
EP  - 789
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
UR  - https://hdl.handle.net/21.15107/rcub_dais_4600
ER  - 
@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "776-789",
volume = "96",
doi = "10.1016/j.msec.2018.11.073",
url = "https://hdl.handle.net/21.15107/rcub_dais_4600"
}
Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C
Elsevier., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073
https://hdl.handle.net/21.15107/rcub_dais_4600
Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073
https://hdl.handle.net/21.15107/rcub_dais_4600 .
Filipović, Nenad, Veselinović, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipič, Metka, Žegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science and Engineering C, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 .,
https://hdl.handle.net/21.15107/rcub_dais_4600 .
1
9
10
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Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(Elsevier, 2019)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/4590
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
SP  - 776
EP  - 789
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
UR  - https://hdl.handle.net/21.15107/rcub_dais_4590
ER  - 
@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "776-789",
volume = "96",
doi = "10.1016/j.msec.2018.11.073",
url = "https://hdl.handle.net/21.15107/rcub_dais_4590"
}
Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C
Elsevier., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073
https://hdl.handle.net/21.15107/rcub_dais_4590
Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073
https://hdl.handle.net/21.15107/rcub_dais_4590 .
Filipović, Nenad, Veselinović, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipič, Metka, Žegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science and Engineering C, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 .,
https://hdl.handle.net/21.15107/rcub_dais_4590 .
1
9
10
12

Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging

Catanzaro, Valeria; Digilio, Giuseppe; Capuana, Federico; Padovan, Sergio; Cutrin, Juan C.; Carniato, Fabio; Porta, Stefano; Grange, Cristina; Filipović, Nenad; Stevanović, Magdalena

(Basel : MDPI, 2019)

TY  - DATA
AU  - Catanzaro, Valeria
AU  - Digilio, Giuseppe
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/7044
AB  - Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)
PB  - Basel : MDPI
T2  - Journal of Functional Biomaterials
T1  - Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
VL  - 10
IS  - 3
UR  - https://hdl.handle.net/21.15107/rcub_dais_7044
ER  - 
@misc{
author = "Catanzaro, Valeria and Digilio, Giuseppe and Capuana, Federico and Padovan, Sergio and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
abstract = "Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)",
publisher = "Basel : MDPI",
journal = "Journal of Functional Biomaterials",
title = "Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging",
volume = "10",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_dais_7044"
}
Catanzaro, V., Digilio, G., Capuana, F., Padovan, S., Cutrin, J. C., Carniato, F., Porta, S., Grange, C., Filipović, N.,& Stevanović, M.. (2019). Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. in Journal of Functional Biomaterials
Basel : MDPI., 10(3).
https://hdl.handle.net/21.15107/rcub_dais_7044
Catanzaro V, Digilio G, Capuana F, Padovan S, Cutrin JC, Carniato F, Porta S, Grange C, Filipović N, Stevanović M. Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. in Journal of Functional Biomaterials. 2019;10(3).
https://hdl.handle.net/21.15107/rcub_dais_7044 .
Catanzaro, Valeria, Digilio, Giuseppe, Capuana, Federico, Padovan, Sergio, Cutrin, Juan C., Carniato, Fabio, Porta, Stefano, Grange, Cristina, Filipović, Nenad, Stevanović, Magdalena, "Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging" in Journal of Functional Biomaterials, 10, no. 3 (2019),
https://hdl.handle.net/21.15107/rcub_dais_7044 .

Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging

Catanzaro, Valeria; Digilio, Giuseppe; Capuana, Federico; Padovan, Sergio; Cutrin, Juan C.; Carniato, Fabio; Porta, Stefano; Grange, Cristina; Filipović, Nenad; Stevanović, Magdalena

(Basel : MDPI, 2019)

TY  - JOUR
AU  - Catanzaro, Valeria
AU  - Digilio, Giuseppe
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/6689
AB  - Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.
PB  - Basel : MDPI
T2  - Journal of Functional Biomaterials
T1  - Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
SP  - 28
VL  - 10
IS  - 3
DO  - 10.3390/jfb10030028
UR  - https://hdl.handle.net/21.15107/rcub_dais_6689
ER  - 
@article{
author = "Catanzaro, Valeria and Digilio, Giuseppe and Capuana, Federico and Padovan, Sergio and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
abstract = "Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.",
publisher = "Basel : MDPI",
journal = "Journal of Functional Biomaterials",
title = "Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging",
pages = "28",
volume = "10",
number = "3",
doi = "10.3390/jfb10030028",
url = "https://hdl.handle.net/21.15107/rcub_dais_6689"
}
Catanzaro, V., Digilio, G., Capuana, F., Padovan, S., Cutrin, J. C., Carniato, F., Porta, S., Grange, C., Filipović, N.,& Stevanović, M.. (2019). Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. in Journal of Functional Biomaterials
Basel : MDPI., 10(3), 28.
https://doi.org/10.3390/jfb10030028
https://hdl.handle.net/21.15107/rcub_dais_6689
Catanzaro V, Digilio G, Capuana F, Padovan S, Cutrin JC, Carniato F, Porta S, Grange C, Filipović N, Stevanović M. Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. in Journal of Functional Biomaterials. 2019;10(3):28.
doi:10.3390/jfb10030028
https://hdl.handle.net/21.15107/rcub_dais_6689 .
Catanzaro, Valeria, Digilio, Giuseppe, Capuana, Federico, Padovan, Sergio, Cutrin, Juan C., Carniato, Fabio, Porta, Stefano, Grange, Cristina, Filipović, Nenad, Stevanović, Magdalena, "Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging" in Journal of Functional Biomaterials, 10, no. 3 (2019):28,
https://doi.org/10.3390/jfb10030028 .,
https://hdl.handle.net/21.15107/rcub_dais_6689 .
3
3
3

Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives

Stevanović, Magdalena; Lukić, Miodrag J.; Stanković, Ana; Filipović, Nenad; Kuzmanović, Maja; Janićijević, Željko

(Elsevier, 2019)

TY  - CHAP
AU  - Stevanović, Magdalena
AU  - Lukić, Miodrag J.
AU  - Stanković, Ana
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Janićijević, Željko
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/B9780081028148000019
UR  - https://dais.sanu.ac.rs/123456789/5760
AB  - Nanotechnology has great potential in the biomedical field. Among other nanomaterials, inorganic nanoparticles have become extremely important since they possess unique physicochemical properties influenced by their specific surface structure. Consequently, inorganic nanoparticles exhibit enhanced functionalities such as biological response, antibacterial and antiviral properties, as well as optical, magnetic, and electrical responses. They have found applications in medicine, pharmacy, controlled drug delivery, optics, electronics, etc. In this chapter, reports on obtaining different metallic and ceramic inorganic nanoparticles such as gold, silver, selenium, copper, iron, zinc oxide, and hydroxyapatite for biomedical applications will be addressed. For each of these nanosystems, the main challenges regarding the currently achieved functional properties and further perspectives will also be presented.
PB  - Elsevier
T2  - Materials for Biomedical Engineering
T1  - Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives
SP  - 1
EP  - 46
DO  - 10.1016/B978-0-08-102814-8.00001-9
UR  - https://hdl.handle.net/21.15107/rcub_dais_5760
ER  - 
@inbook{
author = "Stevanović, Magdalena and Lukić, Miodrag J. and Stanković, Ana and Filipović, Nenad and Kuzmanović, Maja and Janićijević, Željko",
year = "2019",
abstract = "Nanotechnology has great potential in the biomedical field. Among other nanomaterials, inorganic nanoparticles have become extremely important since they possess unique physicochemical properties influenced by their specific surface structure. Consequently, inorganic nanoparticles exhibit enhanced functionalities such as biological response, antibacterial and antiviral properties, as well as optical, magnetic, and electrical responses. They have found applications in medicine, pharmacy, controlled drug delivery, optics, electronics, etc. In this chapter, reports on obtaining different metallic and ceramic inorganic nanoparticles such as gold, silver, selenium, copper, iron, zinc oxide, and hydroxyapatite for biomedical applications will be addressed. For each of these nanosystems, the main challenges regarding the currently achieved functional properties and further perspectives will also be presented.",
publisher = "Elsevier",
journal = "Materials for Biomedical Engineering",
booktitle = "Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives",
pages = "1-46",
doi = "10.1016/B978-0-08-102814-8.00001-9",
url = "https://hdl.handle.net/21.15107/rcub_dais_5760"
}
Stevanović, M., Lukić, M. J., Stanković, A., Filipović, N., Kuzmanović, M.,& Janićijević, Ž.. (2019). Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives. in Materials for Biomedical Engineering
Elsevier., 1-46.
https://doi.org/10.1016/B978-0-08-102814-8.00001-9
https://hdl.handle.net/21.15107/rcub_dais_5760
Stevanović M, Lukić MJ, Stanković A, Filipović N, Kuzmanović M, Janićijević Ž. Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives. in Materials for Biomedical Engineering. 2019;:1-46.
doi:10.1016/B978-0-08-102814-8.00001-9
https://hdl.handle.net/21.15107/rcub_dais_5760 .
Stevanović, Magdalena, Lukić, Miodrag J., Stanković, Ana, Filipović, Nenad, Kuzmanović, Maja, Janićijević, Željko, "Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives" in Materials for Biomedical Engineering (2019):1-46,
https://doi.org/10.1016/B978-0-08-102814-8.00001-9 .,
https://hdl.handle.net/21.15107/rcub_dais_5760 .
2
3

Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels

Padovan, Sergio; Catanzaro, Valeria; Capuana, Federico; Grange, Cristina; Koni, Malvina; Carrera, Carla; Filipović, Nenad; Stevanović, Magdalena

(2019)

TY  - CONF
AU  - Padovan, Sergio
AU  - Catanzaro, Valeria
AU  - Capuana, Federico
AU  - Grange, Cristina
AU  - Koni, Malvina
AU  - Carrera, Carla
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://eventclass.org/contxt_emim2019/online-program/session?s=PS+22#e83
UR  - https://dais.sanu.ac.rs/123456789/5251
AB  - *Introduction*In regenerative medicine, biocompatible hydrogels are increasingly used to encapsulate therapeutic cells prior to transplantation into the host to enhance their long term survival. Cell embedding within bioengineered hydrogels can shield cells from immune response and provide an optimal life-sustaining microenvironment to therapeutic cells. In addition, cell embedding offers the outstanding opportunity to insert microenvironment-responsive imaging labels within the hydrogel, paving the way for non-invasive monitoring of the extracellular microenvironment within the hydrogel. We have inserted redox-responsive MRI labels within cell-embedding hydrogels to follow-up the microenvironment redox state.*Methods*High molecular weight chitosan polymers were chemically conjugated with a Gd-HPDO3A-chelate through a disulfide bond, and interspersed within alginate-based hydrogel capsules. Human mesenchymal stem cells (hMSCs) as model therapeutic cells were embedded into such imaging labelled hydrogel. Embedded cells were incubated under simulated hypoxiaconditions, while being followed-up by T1-weighted MRI at 7T.*Results*Under reducing conditions, reductive cleavage of the disulfide bond in the Gd-chitosan probe yields a low molecular weight Gd-chelate that eventually diffuses out of the hydrogel capsule. The resulting change of MRI contrast enhancement along time is very sensitive to the oxygenation level within cell capsules. The kinetics of clearance of contrast enhancement is an indirect indicator of the survival of encapsulated cells.*Conclusions*The Gd-chitosan probe we developed is promising to follow-up non-invasively the redox microenvironment within cellembedding hydrogels. This approach will find useful application to monitor whether transplanted cells succeed to restore normal tissue oxygenation levels, especially in regenerative medicine approaches to ischemic diseases.
C3  - European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program
T1  - Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels
UR  - https://hdl.handle.net/21.15107/rcub_dais_5251
ER  - 
@conference{
author = "Padovan, Sergio and Catanzaro, Valeria and Capuana, Federico and Grange, Cristina and Koni, Malvina and Carrera, Carla and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
abstract = "*Introduction*In regenerative medicine, biocompatible hydrogels are increasingly used to encapsulate therapeutic cells prior to transplantation into the host to enhance their long term survival. Cell embedding within bioengineered hydrogels can shield cells from immune response and provide an optimal life-sustaining microenvironment to therapeutic cells. In addition, cell embedding offers the outstanding opportunity to insert microenvironment-responsive imaging labels within the hydrogel, paving the way for non-invasive monitoring of the extracellular microenvironment within the hydrogel. We have inserted redox-responsive MRI labels within cell-embedding hydrogels to follow-up the microenvironment redox state.*Methods*High molecular weight chitosan polymers were chemically conjugated with a Gd-HPDO3A-chelate through a disulfide bond, and interspersed within alginate-based hydrogel capsules. Human mesenchymal stem cells (hMSCs) as model therapeutic cells were embedded into such imaging labelled hydrogel. Embedded cells were incubated under simulated hypoxiaconditions, while being followed-up by T1-weighted MRI at 7T.*Results*Under reducing conditions, reductive cleavage of the disulfide bond in the Gd-chitosan probe yields a low molecular weight Gd-chelate that eventually diffuses out of the hydrogel capsule. The resulting change of MRI contrast enhancement along time is very sensitive to the oxygenation level within cell capsules. The kinetics of clearance of contrast enhancement is an indirect indicator of the survival of encapsulated cells.*Conclusions*The Gd-chitosan probe we developed is promising to follow-up non-invasively the redox microenvironment within cellembedding hydrogels. This approach will find useful application to monitor whether transplanted cells succeed to restore normal tissue oxygenation levels, especially in regenerative medicine approaches to ischemic diseases.",
journal = "European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program",
title = "Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels",
url = "https://hdl.handle.net/21.15107/rcub_dais_5251"
}
Padovan, S., Catanzaro, V., Capuana, F., Grange, C., Koni, M., Carrera, C., Filipović, N.,& Stevanović, M.. (2019). Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels. in European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program.
https://hdl.handle.net/21.15107/rcub_dais_5251
Padovan S, Catanzaro V, Capuana F, Grange C, Koni M, Carrera C, Filipović N, Stevanović M. Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels. in European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program. 2019;.
https://hdl.handle.net/21.15107/rcub_dais_5251 .
Padovan, Sergio, Catanzaro, Valeria, Capuana, Federico, Grange, Cristina, Koni, Malvina, Carrera, Carla, Filipović, Nenad, Stevanović, Magdalena, "Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels" in European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program (2019),
https://hdl.handle.net/21.15107/rcub_dais_5251 .

Sinteza i karakterizacija biokompozita poli (є-kaprolakton) / nanočestice selena

Filipović, Nenad

(Univerzitet u Beogradu, Fakultet za fizičku hemiju, 2018)

TY  - THES
AU  - Filipović, Nenad
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6260
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18889/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50755599
UR  - http://nardus.mpn.gov.rs/123456789/10286
UR  - https://dais.sanu.ac.rs/123456789/4589
AB  - Ova doktorska disertacija predstavlja multidisciplinarno istraživanje u okviru kojeg su utvrđeni optimalni uslovi sinteze sfernih čestica poli (ε-kaprolaktona) (PCL-a) sa inkorporiranim, sintetisanim nanočesticama selena; izvršena je detaljna karakterizacija novodobijenog biokompozita različitim metodama fizičkohemijske analize; i ispitana su biološka svojstva značajna za njegovu potencijalnu primenu. Istraživanja su realizovana kroz tri faze...
AB  - This doctoral dissertation represents a multidisciplinary study in which optimal synthesis conditions of poly-(ε-caprolactone) (PCL) spherical particles with incorporated selenium nanoparticles were determined, comprehensive characterization of obtained systems by various physicochemical methods was conducted and investigations of biological properties significant for the potential application of this biocomposite material were performed. In order to complete these tasks investigations were carried out into three consequent stages...
PB  - Univerzitet u Beogradu, Fakultet za fizičku hemiju
T2  - Универзитет у Београду
T1  - Sinteza i karakterizacija biokompozita poli (є-kaprolakton) / nanočestice selena
UR  - https://hdl.handle.net/21.15107/rcub_dais_4589
ER  - 
@phdthesis{
author = "Filipović, Nenad",
year = "2018",
abstract = "Ova doktorska disertacija predstavlja multidisciplinarno istraživanje u okviru kojeg su utvrđeni optimalni uslovi sinteze sfernih čestica poli (ε-kaprolaktona) (PCL-a) sa inkorporiranim, sintetisanim nanočesticama selena; izvršena je detaljna karakterizacija novodobijenog biokompozita različitim metodama fizičkohemijske analize; i ispitana su biološka svojstva značajna za njegovu potencijalnu primenu. Istraživanja su realizovana kroz tri faze..., This doctoral dissertation represents a multidisciplinary study in which optimal synthesis conditions of poly-(ε-caprolactone) (PCL) spherical particles with incorporated selenium nanoparticles were determined, comprehensive characterization of obtained systems by various physicochemical methods was conducted and investigations of biological properties significant for the potential application of this biocomposite material were performed. In order to complete these tasks investigations were carried out into three consequent stages...",
publisher = "Univerzitet u Beogradu, Fakultet za fizičku hemiju",
journal = "Универзитет у Београду",
title = "Sinteza i karakterizacija biokompozita poli (є-kaprolakton) / nanočestice selena",
url = "https://hdl.handle.net/21.15107/rcub_dais_4589"
}
Filipović, N.. (2018). Sinteza i karakterizacija biokompozita poli (є-kaprolakton) / nanočestice selena. in Универзитет у Београду
Univerzitet u Beogradu, Fakultet za fizičku hemiju..
https://hdl.handle.net/21.15107/rcub_dais_4589
Filipović N. Sinteza i karakterizacija biokompozita poli (є-kaprolakton) / nanočestice selena. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_dais_4589 .
Filipović, Nenad, "Sinteza i karakterizacija biokompozita poli (є-kaprolakton) / nanočestice selena" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_dais_4589 .

Biodegradable microparticles as scaffolds for cell therapy

Filipović, Nenad; Digilio, Giuseppe; Catanzaro, Valeria; Capuana, Federico; Cutrin, Juan C.; Carniato, Fabio; Porta, Stefano; Grange, Cristina; Stevanović, Magdalena

(Belgrade : Institute of Technical Sciences of SASA, 2018)

TY  - CONF
AU  - Filipović, Nenad
AU  - Digilio, Giuseppe
AU  - Catanzaro, Valeria
AU  - Capuana, Federico
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Stevanović, Magdalena
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4721
AB  - Cell therapy is promising strategy that has attracted a lot of attention recently regarding regeneration of diverse tissues and treatment of various pathological conditions. Despite its great potential, several issues still need to be addressed. Among them administration route and dose, microenvironment conditions and host immune response are recognized as a major causes which lead to cells transplantation failure. In this work it is presented novel microstructural system based on biodegradable polymer poly(lactide-co-glycolide) (PLGA) and combination of biocompatible polyvinyl alcohol (PVA) and chitosan, as a scaffold for human mesenchymal stem cells (hMSCs) growth. The obtained microparticles with diameter 200-600 μm showed full biocompatibility with human hMSCs. Besides serving as a solid support, polymeric particles provided controlled release of contrast agent - gadolinium fluoride nanoparticles (Gd-NP) up to 5 weeks. The release of Gd-NP is enhanced by acidic conditions. Magnetic Resonance Imaging (MRI) of the samples embedded in 1% agar showed that contrast enhancement in T1-weighted (T1w) MR images is influenced by the amount of released Gd-NP. Based on these preliminary results, presented theranostic system could be considered for cells grafting.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
T1  - Biodegradable microparticles as scaffolds for cell therapy
SP  - 7
EP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_dais_4721
ER  - 
@conference{
author = "Filipović, Nenad and Digilio, Giuseppe and Catanzaro, Valeria and Capuana, Federico and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Stevanović, Magdalena",
year = "2018",
abstract = "Cell therapy is promising strategy that has attracted a lot of attention recently regarding regeneration of diverse tissues and treatment of various pathological conditions. Despite its great potential, several issues still need to be addressed. Among them administration route and dose, microenvironment conditions and host immune response are recognized as a major causes which lead to cells transplantation failure. In this work it is presented novel microstructural system based on biodegradable polymer poly(lactide-co-glycolide) (PLGA) and combination of biocompatible polyvinyl alcohol (PVA) and chitosan, as a scaffold for human mesenchymal stem cells (hMSCs) growth. The obtained microparticles with diameter 200-600 μm showed full biocompatibility with human hMSCs. Besides serving as a solid support, polymeric particles provided controlled release of contrast agent - gadolinium fluoride nanoparticles (Gd-NP) up to 5 weeks. The release of Gd-NP is enhanced by acidic conditions. Magnetic Resonance Imaging (MRI) of the samples embedded in 1% agar showed that contrast enhancement in T1-weighted (T1w) MR images is influenced by the amount of released Gd-NP. Based on these preliminary results, presented theranostic system could be considered for cells grafting.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia",
title = "Biodegradable microparticles as scaffolds for cell therapy",
pages = "7-7",
url = "https://hdl.handle.net/21.15107/rcub_dais_4721"
}
Filipović, N., Digilio, G., Catanzaro, V., Capuana, F., Cutrin, J. C., Carniato, F., Porta, S., Grange, C.,& Stevanović, M.. (2018). Biodegradable microparticles as scaffolds for cell therapy. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 7-7.
https://hdl.handle.net/21.15107/rcub_dais_4721
Filipović N, Digilio G, Catanzaro V, Capuana F, Cutrin JC, Carniato F, Porta S, Grange C, Stevanović M. Biodegradable microparticles as scaffolds for cell therapy. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia. 2018;:7-7.
https://hdl.handle.net/21.15107/rcub_dais_4721 .
Filipović, Nenad, Digilio, Giuseppe, Catanzaro, Valeria, Capuana, Federico, Cutrin, Juan C., Carniato, Fabio, Porta, Stefano, Grange, Cristina, Stevanović, Magdalena, "Biodegradable microparticles as scaffolds for cell therapy" in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia (2018):7-7,
https://hdl.handle.net/21.15107/rcub_dais_4721 .

Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants

Capuana, Federico; Padovan, Sergio; Grange, Cristina; Catanzaro, Valeria; Cutrin, J. C.; Stevanović, Magdalena; Filipović, Nenad; Digilio, G.

(European Society for Molecular Imaging, 2018)

TY  - CONF
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Grange, Cristina
AU  - Catanzaro, Valeria
AU  - Cutrin, J. C.
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Digilio, G.
PY  - 2018
UR  - http://eventclass.org/contxt_emim2018/online-program/session?s=101#153
UR  - https://dais.sanu.ac.rs/123456789/4667
AB  - Introduction: Cell encapsulation by hydrogels is intended to shield transplanted cells from the host hostile environment by preventing the infiltration of host immune cells. Cell scaffolding by solid biocompatible microparticles is intended to provide a structural support to implanted cells and to mimic the extracellular matrix, allowing cells to proliferate and/or differentiate in the desired way. We present strategies to label scaffolding biomaterials with microenvironment responsive MRI probes, for applications in the follow-up of cell transplants.

Methods: Microparticles (MPs) based on PLGA/chitosan were incorporated with gadolinium fluoride nanoparticles (GdNPs), as the MRI T1-contrast agent. The system is designed such to release Gd-NPs in the extracellular matrix (ECM), thus activating MRI contrast, unless MPs are attacked by the immune system (Foreign Body Response, FBR). To proof the concept, PLGA-based MPs were seeded with hMSCs and implanted into either immunocompetent or immunocompromised mice, and the transplants were followed-up by MRI for three weeks. Ex-vivo histologic assessment was carried out at the end of the follow-up.

Results/Discussion: Immunocompetent mice showed poor activation, if any, of MRI contrast within the cell graft. Immunocompromised mice, on the other hand, showed a progressive activation of MRI contrast. Ex-vivo histology showed extensive FBR directed against microparticles in immunocompetent mice, with some surviving hMSCs in the ECM but not on the scaffold surface. No significant FBR was detected in immunocompromised mice, and hMSCs were still adhering to the scaffolds.

Conclusions: The proposed system is able to assess whether or not cell grafts are subjected to innate immune response, an event that is likely correlated to the loss of transplanted cells.
PB  - European Society for Molecular Imaging
C3  - European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program
T1  - Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants
UR  - https://hdl.handle.net/21.15107/rcub_dais_4667
ER  - 
@conference{
author = "Capuana, Federico and Padovan, Sergio and Grange, Cristina and Catanzaro, Valeria and Cutrin, J. C. and Stevanović, Magdalena and Filipović, Nenad and Digilio, G.",
year = "2018",
abstract = "Introduction: Cell encapsulation by hydrogels is intended to shield transplanted cells from the host hostile environment by preventing the infiltration of host immune cells. Cell scaffolding by solid biocompatible microparticles is intended to provide a structural support to implanted cells and to mimic the extracellular matrix, allowing cells to proliferate and/or differentiate in the desired way. We present strategies to label scaffolding biomaterials with microenvironment responsive MRI probes, for applications in the follow-up of cell transplants.

Methods: Microparticles (MPs) based on PLGA/chitosan were incorporated with gadolinium fluoride nanoparticles (GdNPs), as the MRI T1-contrast agent. The system is designed such to release Gd-NPs in the extracellular matrix (ECM), thus activating MRI contrast, unless MPs are attacked by the immune system (Foreign Body Response, FBR). To proof the concept, PLGA-based MPs were seeded with hMSCs and implanted into either immunocompetent or immunocompromised mice, and the transplants were followed-up by MRI for three weeks. Ex-vivo histologic assessment was carried out at the end of the follow-up.

Results/Discussion: Immunocompetent mice showed poor activation, if any, of MRI contrast within the cell graft. Immunocompromised mice, on the other hand, showed a progressive activation of MRI contrast. Ex-vivo histology showed extensive FBR directed against microparticles in immunocompetent mice, with some surviving hMSCs in the ECM but not on the scaffold surface. No significant FBR was detected in immunocompromised mice, and hMSCs were still adhering to the scaffolds.

Conclusions: The proposed system is able to assess whether or not cell grafts are subjected to innate immune response, an event that is likely correlated to the loss of transplanted cells.",
publisher = "European Society for Molecular Imaging",
journal = "European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program",
title = "Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants",
url = "https://hdl.handle.net/21.15107/rcub_dais_4667"
}
Capuana, F., Padovan, S., Grange, C., Catanzaro, V., Cutrin, J. C., Stevanović, M., Filipović, N.,& Digilio, G.. (2018). Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants. in European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program
European Society for Molecular Imaging..
https://hdl.handle.net/21.15107/rcub_dais_4667
Capuana F, Padovan S, Grange C, Catanzaro V, Cutrin JC, Stevanović M, Filipović N, Digilio G. Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants. in European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program. 2018;.
https://hdl.handle.net/21.15107/rcub_dais_4667 .
Capuana, Federico, Padovan, Sergio, Grange, Cristina, Catanzaro, Valeria, Cutrin, J. C., Stevanović, Magdalena, Filipović, Nenad, Digilio, G., "Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants" in European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program (2018),
https://hdl.handle.net/21.15107/rcub_dais_4667 .

Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity

Lojpur, Vesna; Krstić, Jelena; Kačarević-Popović, Zorica; Filipović, Nenad; Validžić, Ivana

(Springer, 2018)

TY  - JOUR
AU  - Lojpur, Vesna
AU  - Krstić, Jelena
AU  - Kačarević-Popović, Zorica
AU  - Filipović, Nenad
AU  - Validžić, Ivana
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4637
AB  - Producing green and efficient energy sources is a major challenge. As a consequence, the use of photovoltaic devices for conversion of light into electricity is growing worldwide. A lot of effort had been invested to create high-efficient solar cells, but their durability, stability, flexibility and efficiency at low light intensities are still unexplored. Here, we built a flexible solar cell made of p-doped, amorphized a-undoped and n-doped Sb2S3 solid carrier loaded with electrolyte. Indium tin oxide glass was the working electrode, and aluminium was the counter electrode. Every (p–a–n) flexible Sb2S3/solid carrier layers were obtained using a cheap casting/solvent evaporation technique, from a blend consisted of chitosan, polyethylene glycol and electrolyte containing 0.5 M potassium iodide and 0.05 M iodine, and corresponding synthesized amorphized a-undoped and p and n-doped Sb2S3 semiconductor. Results show that flexible Sb2S3 solar cell possesses good stability and efficiency of about 10% at 5% sun. Overall, our findings demonstrate for the first time that flexible solar cell can be made and used for low light intensity applications. © 2018, Springer International Publishing AG, part of Springer Nature.
PB  - Springer
T2  - Environmental Chemistry Letters
T1  - Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity
SP  - 659
EP  - 664
VL  - 16
IS  - 2
DO  - 10.1007/s10311-017-0702-7
UR  - https://hdl.handle.net/21.15107/rcub_dais_4637
ER  - 
@article{
author = "Lojpur, Vesna and Krstić, Jelena and Kačarević-Popović, Zorica and Filipović, Nenad and Validžić, Ivana",
year = "2018",
abstract = "Producing green and efficient energy sources is a major challenge. As a consequence, the use of photovoltaic devices for conversion of light into electricity is growing worldwide. A lot of effort had been invested to create high-efficient solar cells, but their durability, stability, flexibility and efficiency at low light intensities are still unexplored. Here, we built a flexible solar cell made of p-doped, amorphized a-undoped and n-doped Sb2S3 solid carrier loaded with electrolyte. Indium tin oxide glass was the working electrode, and aluminium was the counter electrode. Every (p–a–n) flexible Sb2S3/solid carrier layers were obtained using a cheap casting/solvent evaporation technique, from a blend consisted of chitosan, polyethylene glycol and electrolyte containing 0.5 M potassium iodide and 0.05 M iodine, and corresponding synthesized amorphized a-undoped and p and n-doped Sb2S3 semiconductor. Results show that flexible Sb2S3 solar cell possesses good stability and efficiency of about 10% at 5% sun. Overall, our findings demonstrate for the first time that flexible solar cell can be made and used for low light intensity applications. © 2018, Springer International Publishing AG, part of Springer Nature.",
publisher = "Springer",
journal = "Environmental Chemistry Letters",
title = "Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity",
pages = "659-664",
volume = "16",
number = "2",
doi = "10.1007/s10311-017-0702-7",
url = "https://hdl.handle.net/21.15107/rcub_dais_4637"
}
Lojpur, V., Krstić, J., Kačarević-Popović, Z., Filipović, N.,& Validžić, I.. (2018). Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity. in Environmental Chemistry Letters
Springer., 16(2), 659-664.
https://doi.org/10.1007/s10311-017-0702-7
https://hdl.handle.net/21.15107/rcub_dais_4637
Lojpur V, Krstić J, Kačarević-Popović Z, Filipović N, Validžić I. Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity. in Environmental Chemistry Letters. 2018;16(2):659-664.
doi:10.1007/s10311-017-0702-7
https://hdl.handle.net/21.15107/rcub_dais_4637 .
Lojpur, Vesna, Krstić, Jelena, Kačarević-Popović, Zorica, Filipović, Nenad, Validžić, Ivana, "Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity" in Environmental Chemistry Letters, 16, no. 2 (2018):659-664,
https://doi.org/10.1007/s10311-017-0702-7 .,
https://hdl.handle.net/21.15107/rcub_dais_4637 .
4
2
4

Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity

Lojpur, Vesna; Krstić, Jelena; Kačarević-Popović, Zorica; Filipović, Nenad; Validžić, Ivana

(Springer, 2018)

TY  - JOUR
AU  - Lojpur, Vesna
AU  - Krstić, Jelena
AU  - Kačarević-Popović, Zorica
AU  - Filipović, Nenad
AU  - Validžić, Ivana
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/3754
AB  - Producing green and efficient energy sources is a major challenge. As a consequence, the use of photovoltaic devices for conversion of light into electricity is growing worldwide. A lot of effort had been invested to create high-efficient solar cells, but their durability, stability, flexibility and efficiency at low light intensities are still unexplored. Here, we built a flexible solar cell made of p-doped, amorphized a-undoped and n-doped Sb2S3 solid carrier loaded with electrolyte. Indium tin oxide glass was the working electrode, and aluminium was the counter electrode. Every (p–a–n) flexible Sb2S3/solid carrier layers were obtained using a cheap casting/solvent evaporation technique, from a blend consisted of chitosan, polyethylene glycol and electrolyte containing 0.5 M potassium iodide and 0.05 M iodine, and corresponding synthesized amorphized a-undoped and p and n-doped Sb2S3 semiconductor. Results show that flexible Sb2S3 solar cell possesses good stability and efficiency of about 10% at 5% sun. Overall, our findings demonstrate for the first time that flexible solar cell can be made and used for low light intensity applications. © 2018, Springer International Publishing AG, part of Springer Nature.
PB  - Springer
T2  - Environmental Chemistry Letters
T1  - Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity
SP  - 659
EP  - 664
VL  - 16
IS  - 2
DO  - 10.1007/s10311-017-0702-7
UR  - https://hdl.handle.net/21.15107/rcub_dais_3754
ER  - 
@article{
author = "Lojpur, Vesna and Krstić, Jelena and Kačarević-Popović, Zorica and Filipović, Nenad and Validžić, Ivana",
year = "2018",
abstract = "Producing green and efficient energy sources is a major challenge. As a consequence, the use of photovoltaic devices for conversion of light into electricity is growing worldwide. A lot of effort had been invested to create high-efficient solar cells, but their durability, stability, flexibility and efficiency at low light intensities are still unexplored. Here, we built a flexible solar cell made of p-doped, amorphized a-undoped and n-doped Sb2S3 solid carrier loaded with electrolyte. Indium tin oxide glass was the working electrode, and aluminium was the counter electrode. Every (p–a–n) flexible Sb2S3/solid carrier layers were obtained using a cheap casting/solvent evaporation technique, from a blend consisted of chitosan, polyethylene glycol and electrolyte containing 0.5 M potassium iodide and 0.05 M iodine, and corresponding synthesized amorphized a-undoped and p and n-doped Sb2S3 semiconductor. Results show that flexible Sb2S3 solar cell possesses good stability and efficiency of about 10% at 5% sun. Overall, our findings demonstrate for the first time that flexible solar cell can be made and used for low light intensity applications. © 2018, Springer International Publishing AG, part of Springer Nature.",
publisher = "Springer",
journal = "Environmental Chemistry Letters",
title = "Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity",
pages = "659-664",
volume = "16",
number = "2",
doi = "10.1007/s10311-017-0702-7",
url = "https://hdl.handle.net/21.15107/rcub_dais_3754"
}
Lojpur, V., Krstić, J., Kačarević-Popović, Z., Filipović, N.,& Validžić, I.. (2018). Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity. in Environmental Chemistry Letters
Springer., 16(2), 659-664.
https://doi.org/10.1007/s10311-017-0702-7
https://hdl.handle.net/21.15107/rcub_dais_3754
Lojpur V, Krstić J, Kačarević-Popović Z, Filipović N, Validžić I. Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity. in Environmental Chemistry Letters. 2018;16(2):659-664.
doi:10.1007/s10311-017-0702-7
https://hdl.handle.net/21.15107/rcub_dais_3754 .
Lojpur, Vesna, Krstić, Jelena, Kačarević-Popović, Zorica, Filipović, Nenad, Validžić, Ivana, "Flexible and high-efficiency Sb2S3/solid carrier solar cell at low light intensity" in Environmental Chemistry Letters, 16, no. 2 (2018):659-664,
https://doi.org/10.1007/s10311-017-0702-7 .,
https://hdl.handle.net/21.15107/rcub_dais_3754 .
4
2
3

Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats

Dinić, Miroslav; Pecikoza, Uroš; Đokić, Jelena; Stepanović Petrović, Radica; Milenković, Marina; Stevanović, Magdalena; Filipović, Nenad; Begović, Jelena; Golić, Nataša; Lukić, Jovanka

(Lausanne : Frontiers, 2018)

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Pecikoza, Uroš
AU  - Đokić, Jelena
AU  - Stepanović Petrović, Radica
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Lukić, Jovanka
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/2347
AB  - The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1β and iNOS mRNAs in rat’s paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1β, TNF-α and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.
PB  - Lausanne : Frontiers
T2  - Frontiers in Pharmacology
T1  - Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats
SP  - Article 1
VL  - 9
DO  - 10.3389/fphar.2018.00001
UR  - https://hdl.handle.net/21.15107/rcub_dais_2347
ER  - 
@article{
author = "Dinić, Miroslav and Pecikoza, Uroš and Đokić, Jelena and Stepanović Petrović, Radica and Milenković, Marina and Stevanović, Magdalena and Filipović, Nenad and Begović, Jelena and Golić, Nataša and Lukić, Jovanka",
year = "2018",
abstract = "The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1β and iNOS mRNAs in rat’s paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1β, TNF-α and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.",
publisher = "Lausanne : Frontiers",
journal = "Frontiers in Pharmacology",
title = "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats",
pages = "Article 1",
volume = "9",
doi = "10.3389/fphar.2018.00001",
url = "https://hdl.handle.net/21.15107/rcub_dais_2347"
}
Dinić, M., Pecikoza, U., Đokić, J., Stepanović Petrović, R., Milenković, M., Stevanović, M., Filipović, N., Begović, J., Golić, N.,& Lukić, J.. (2018). Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology
Lausanne : Frontiers., 9, Article 1.
https://doi.org/10.3389/fphar.2018.00001
https://hdl.handle.net/21.15107/rcub_dais_2347
Dinić M, Pecikoza U, Đokić J, Stepanović Petrović R, Milenković M, Stevanović M, Filipović N, Begović J, Golić N, Lukić J. Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology. 2018;9:Article 1.
doi:10.3389/fphar.2018.00001
https://hdl.handle.net/21.15107/rcub_dais_2347 .
Dinić, Miroslav, Pecikoza, Uroš, Đokić, Jelena, Stepanović Petrović, Radica, Milenković, Marina, Stevanović, Magdalena, Filipović, Nenad, Begović, Jelena, Golić, Nataša, Lukić, Jovanka, "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats" in Frontiers in Pharmacology, 9 (2018):Article 1,
https://doi.org/10.3389/fphar.2018.00001 .,
https://hdl.handle.net/21.15107/rcub_dais_2347 .
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Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums

Filipović, Nenad; Jeremić, Sanja; Đokić, Lidija; Ražić, Slavica; Stevanović, Magdalena

(Belgrade : Institute of Technical Sciences of SASA, 2016)

TY  - CONF
AU  - Filipović, Nenad
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Ražić, Slavica
AU  - Stevanović, Magdalena
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/886
AB  - One of the most prominent properties of poly (є-caprolactone) (PCL) as a biodegradable polymer is slow degradation rate. Due to this advantage the PCL is often used in versatile systems for drug delivery or tissue engineering. When it comes to drug delivery systems, this property of PCL provides the slow release of encapsulated medicaments in order to avoid acute toxicity i.e. to enhance therapeutic efficiency, or protects medicaments from "aggressive" environment and ensures prolonged effect. Selenium nanoparticles (SeNp) recently gained attention as a potential candidate for cancer therapy and prevention with antibacterial properties as well. The major drawback of SeNp is substantial risk of toxicity. Degradation itself is a function of several material properties as well as the nature of surrounding medium. In this work it is examined the release of SeNp from PCL microparticles during the degradation in four different mediums: phosphate buffered saline (PBS), solution of lipase isolated from porcine pancreas in PBS, 0.1 M hydrochloric acid (HCL) and Psseudomonas aeruginosa cell free extract in PBS. The main idea was to compare the release of the selenium nanoparticles in physiological conditions (the first three medium) and in the pathological conditions (the fourth medium), respectively. Firstly, the PCL/SeNp were suspended in adequate medium and placed in water bath at 37 °C. At exact times, samples were collected and examined by different techniques: X-ray diffraction (XRD), inductively coupled plasma-atomic emission spectroscopy (ICP-AES), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), differential scanning calorimetry (DSC). The release of selenium nanoparticles in physiological conditions occurred in a very slow manner without burst release while in the presence of bacterial extract the release was much more pronounced, even after 24 h.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade
T1  - Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums
SP  - 8
EP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_dais_886
ER  - 
@conference{
author = "Filipović, Nenad and Jeremić, Sanja and Đokić, Lidija and Ražić, Slavica and Stevanović, Magdalena",
year = "2016",
abstract = "One of the most prominent properties of poly (є-caprolactone) (PCL) as a biodegradable polymer is slow degradation rate. Due to this advantage the PCL is often used in versatile systems for drug delivery or tissue engineering. When it comes to drug delivery systems, this property of PCL provides the slow release of encapsulated medicaments in order to avoid acute toxicity i.e. to enhance therapeutic efficiency, or protects medicaments from "aggressive" environment and ensures prolonged effect. Selenium nanoparticles (SeNp) recently gained attention as a potential candidate for cancer therapy and prevention with antibacterial properties as well. The major drawback of SeNp is substantial risk of toxicity. Degradation itself is a function of several material properties as well as the nature of surrounding medium. In this work it is examined the release of SeNp from PCL microparticles during the degradation in four different mediums: phosphate buffered saline (PBS), solution of lipase isolated from porcine pancreas in PBS, 0.1 M hydrochloric acid (HCL) and Psseudomonas aeruginosa cell free extract in PBS. The main idea was to compare the release of the selenium nanoparticles in physiological conditions (the first three medium) and in the pathological conditions (the fourth medium), respectively. Firstly, the PCL/SeNp were suspended in adequate medium and placed in water bath at 37 °C. At exact times, samples were collected and examined by different techniques: X-ray diffraction (XRD), inductively coupled plasma-atomic emission spectroscopy (ICP-AES), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), differential scanning calorimetry (DSC). The release of selenium nanoparticles in physiological conditions occurred in a very slow manner without burst release while in the presence of bacterial extract the release was much more pronounced, even after 24 h.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade",
title = "Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums",
pages = "8-8",
url = "https://hdl.handle.net/21.15107/rcub_dais_886"
}
Filipović, N., Jeremić, S., Đokić, L., Ražić, S.,& Stevanović, M.. (2016). Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums. in Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade
Belgrade : Institute of Technical Sciences of SASA., 8-8.
https://hdl.handle.net/21.15107/rcub_dais_886
Filipović N, Jeremić S, Đokić L, Ražić S, Stevanović M. Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums. in Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade. 2016;:8-8.
https://hdl.handle.net/21.15107/rcub_dais_886 .
Filipović, Nenad, Jeremić, Sanja, Đokić, Lidija, Ražić, Slavica, Stevanović, Magdalena, "Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums" in Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade (2016):8-8,
https://hdl.handle.net/21.15107/rcub_dais_886 .

45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity

Stevanović, Magdalena; Filipović, Nenad; Đurđević, Jelena; Lukić, Miodrag J.; Milenković, Marina; Boccaccini, Aldo

(2015)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Đurđević, Jelena
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
AU  - Boccaccini, Aldo
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/4670
AB  - n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity
SP  - 208
EP  - 215
VL  - 132
DO  - 10.1016/j.colsurfb.2015.05.024
UR  - https://hdl.handle.net/21.15107/rcub_dais_4670
ER  - 
@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Đurđević, Jelena and Lukić, Miodrag J. and Milenković, Marina and Boccaccini, Aldo",
year = "2015",
abstract = "n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity",
pages = "208-215",
volume = "132",
doi = "10.1016/j.colsurfb.2015.05.024",
url = "https://hdl.handle.net/21.15107/rcub_dais_4670"
}
Stevanović, M., Filipović, N., Đurđević, J., Lukić, M. J., Milenković, M.,& Boccaccini, A.. (2015). 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. in Colloids and Surfaces B: Biointerfaces, 132, 208-215.
https://doi.org/10.1016/j.colsurfb.2015.05.024
https://hdl.handle.net/21.15107/rcub_dais_4670
Stevanović M, Filipović N, Đurđević J, Lukić MJ, Milenković M, Boccaccini A. 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. in Colloids and Surfaces B: Biointerfaces. 2015;132:208-215.
doi:10.1016/j.colsurfb.2015.05.024
https://hdl.handle.net/21.15107/rcub_dais_4670 .
Stevanović, Magdalena, Filipović, Nenad, Đurđević, Jelena, Lukić, Miodrag J., Milenković, Marina, Boccaccini, Aldo, "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity" in Colloids and Surfaces B: Biointerfaces, 132 (2015):208-215,
https://doi.org/10.1016/j.colsurfb.2015.05.024 .,
https://hdl.handle.net/21.15107/rcub_dais_4670 .
1
59
52
53

Selenium nanoparticles as a potential candidate in cancer treatment

Filipović, Nenad; Ninić, Jana; Filipič, Metka; Filipović, Miloš; Stevanović, Magdalena

(Rovinj : International Association of Physical Chemists, 2015)

TY  - CONF
AU  - Filipović, Nenad
AU  - Ninić, Jana
AU  - Filipič, Metka
AU  - Filipović, Miloš
AU  - Stevanović, Magdalena
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/858
AB  - The broad spectrum of selenium applications in pharmacy and medicine has been known for a while and strongly depends on its chemical form, size and shape. However, the use of Se often requires consumption over the long period, so the toxicity of Se is always a crucial concern. Majority of available pharmaceutical products contain organic forms of selenium or its salts, but recently, when it comes to cancer treatment, elemental selenium nanoparticles (SeNPs) have emerged as a novel selenium source with the advantage of reduced risk of selenium toxicity, but with same bioavailability and efficacy in increasing the activities of selenoenzimes. 
In this work we are presenting the fast, reproducible method for producing stable colloidal suspension of amorphous SeNPs (<80 nm). These SeNPS were successful incorporated within PCL microspheres by combining the high speed homogenization and the precipitation in a solvent/non-solvent system. The obtained PCL/SeNPs were characterized by Fourier transform infrared spectroscopy (FTIR), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and thermal analysis methods (TGA-DTA). The cytotoxicity and the formation of intracellular reactive oxygen species of SeNPs as well as of PCL/SeNPs were investigated employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and using a fluorescent probe (DCFDA test) respectively. Both systems have shown good biocompatibility. The anticancer activity of SeNPs was examined on the HeLa cell line and it was demonstrated that SeNPs exhibits strong, a dose dependent, anticancer activity by preventing further HeLa cells growth and division. Bearing in mind that PCL is well known biodegradable polymer with low degradation rate, it is our opinion that PCL/SeNPs possess a great potential for cancer treatment.
PB  - Rovinj : International Association of Physical Chemists
C3  - Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK
T1  - Selenium nanoparticles as a potential candidate in cancer treatment
UR  - https://hdl.handle.net/21.15107/rcub_dais_858
ER  - 
@conference{
author = "Filipović, Nenad and Ninić, Jana and Filipič, Metka and Filipović, Miloš and Stevanović, Magdalena",
year = "2015",
abstract = "The broad spectrum of selenium applications in pharmacy and medicine has been known for a while and strongly depends on its chemical form, size and shape. However, the use of Se often requires consumption over the long period, so the toxicity of Se is always a crucial concern. Majority of available pharmaceutical products contain organic forms of selenium or its salts, but recently, when it comes to cancer treatment, elemental selenium nanoparticles (SeNPs) have emerged as a novel selenium source with the advantage of reduced risk of selenium toxicity, but with same bioavailability and efficacy in increasing the activities of selenoenzimes. 
In this work we are presenting the fast, reproducible method for producing stable colloidal suspension of amorphous SeNPs (<80 nm). These SeNPS were successful incorporated within PCL microspheres by combining the high speed homogenization and the precipitation in a solvent/non-solvent system. The obtained PCL/SeNPs were characterized by Fourier transform infrared spectroscopy (FTIR), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and thermal analysis methods (TGA-DTA). The cytotoxicity and the formation of intracellular reactive oxygen species of SeNPs as well as of PCL/SeNPs were investigated employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and using a fluorescent probe (DCFDA test) respectively. Both systems have shown good biocompatibility. The anticancer activity of SeNPs was examined on the HeLa cell line and it was demonstrated that SeNPs exhibits strong, a dose dependent, anticancer activity by preventing further HeLa cells growth and division. Bearing in mind that PCL is well known biodegradable polymer with low degradation rate, it is our opinion that PCL/SeNPs possess a great potential for cancer treatment.",
publisher = "Rovinj : International Association of Physical Chemists",
journal = "Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK",
title = "Selenium nanoparticles as a potential candidate in cancer treatment",
url = "https://hdl.handle.net/21.15107/rcub_dais_858"
}
Filipović, N., Ninić, J., Filipič, M., Filipović, M.,& Stevanović, M.. (2015). Selenium nanoparticles as a potential candidate in cancer treatment. in Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK
Rovinj : International Association of Physical Chemists..
https://hdl.handle.net/21.15107/rcub_dais_858
Filipović N, Ninić J, Filipič M, Filipović M, Stevanović M. Selenium nanoparticles as a potential candidate in cancer treatment. in Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK. 2015;.
https://hdl.handle.net/21.15107/rcub_dais_858 .
Filipović, Nenad, Ninić, Jana, Filipič, Metka, Filipović, Miloš, Stevanović, Magdalena, "Selenium nanoparticles as a potential candidate in cancer treatment" in Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK (2015),
https://hdl.handle.net/21.15107/rcub_dais_858 .