TY  - JOUR
AU  - Vukelić, Marija Đ.
AU  - Mitić, Žarko
AU  - Miljković, Miroslav
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Živanov Čurlis, Jelena Z.
AU  - Vasiljević, Perica
AU  - Najman, Stevo
PY  - 2012
UR  - https://dais.sanu.ac.rs/123456789/489
AB  - Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.
PB  - Milano : Wichtig Publishing
T2  - Journal of Applied Biomaterials and Functional Materials
T1  - Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid
SP  - 43
EP  - 48
DO  - 10.5301/JABFM.2012.9274
UR  - https://hdl.handle.net/21.15107/rcub_dais_489
ER  - 

@article{
author = "Vukelić, Marija Đ. and Mitić, Žarko and Miljković, Miroslav and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Živanov Čurlis, Jelena Z. and Vasiljević, Perica and Najman, Stevo",
year = "2012",
abstract = "Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.",
publisher = "Milano : Wichtig Publishing",
journal = "Journal of Applied Biomaterials and Functional Materials",
title = "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid",
pages = "43-48",
doi = "10.5301/JABFM.2012.9274",
url = "https://hdl.handle.net/21.15107/rcub_dais_489"
}

Vukelić, M. Đ., Mitić, Ž., Miljković, M., Živković, J. M., Ignjatović, N., Uskoković, D., Živanov Čurlis, J. Z., Vasiljević, P.,& Najman, S.. (2012). Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials
Milano : Wichtig Publishing., 43-48.
https://doi.org/10.5301/JABFM.2012.9274
https://hdl.handle.net/21.15107/rcub_dais_489

Vukelić MĐ, Mitić Ž, Miljković M, Živković JM, Ignjatović N, Uskoković D, Živanov Čurlis JZ, Vasiljević P, Najman S. Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials. 2012;:43-48.
doi:10.5301/JABFM.2012.9274
https://hdl.handle.net/21.15107/rcub_dais_489 .

Vukelić, Marija Đ., Mitić, Žarko, Miljković, Miroslav, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Živanov Čurlis, Jelena Z., Vasiljević, Perica, Najman, Stevo, "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid" in Journal of Applied Biomaterials and Functional Materials (2012):43-48,
https://doi.org/10.5301/JABFM.2012.9274 .,
https://hdl.handle.net/21.15107/rcub_dais_489 .

TY  - JOUR
AU  - Vasiljević, Perica
AU  - Najman, Stevo
AU  - Đorđević, Ljubiša
AU  - Savić, Vojin P.
AU  - Vukelić, Marija Đ.
AU  - Živanov Čurlis, Jelena Z.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2009
UR  - http://www.ncbi.nlm.nih.gov/pubmed/0367-598X0904301V
UR  - https://dais.sanu.ac.rs/123456789/3362
AB  - Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone.
AB  - Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.
PB  - Belgrade : Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold
T1  - Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA
SP  - 301
EP  - 307
VL  - 63
IS  - 4
DO  - 10.2298/HEMIND0904301V
UR  - https://hdl.handle.net/21.15107/rcub_dais_3362
ER  - 

@article{
author = "Vasiljević, Perica and Najman, Stevo and Đorđević, Ljubiša and Savić, Vojin P. and Vukelić, Marija Đ. and Živanov Čurlis, Jelena Z. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2009",
abstract = "Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone., Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.",
publisher = "Belgrade : Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold, Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA",
pages = "301-307",
volume = "63",
number = "4",
doi = "10.2298/HEMIND0904301V",
url = "https://hdl.handle.net/21.15107/rcub_dais_3362"
}

Vasiljević, P., Najman, S., Đorđević, L., Savić, V. P., Vukelić, M. Đ., Živanov Čurlis, J. Z., Ignjatović, N.,& Uskoković, D.. (2009). Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold. in Hemijska industrija
Belgrade : Association of the Chemical Engineers of Serbia., 63(4), 301-307.
https://doi.org/10.2298/HEMIND0904301V
https://hdl.handle.net/21.15107/rcub_dais_3362

Vasiljević P, Najman S, Đorđević L, Savić VP, Vukelić MĐ, Živanov Čurlis JZ, Ignjatović N, Uskoković D. Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold. in Hemijska industrija. 2009;63(4):301-307.
doi:10.2298/HEMIND0904301V
https://hdl.handle.net/21.15107/rcub_dais_3362 .

Vasiljević, Perica, Najman, Stevo, Đorđević, Ljubiša, Savić, Vojin P., Vukelić, Marija Đ., Živanov Čurlis, Jelena Z., Ignjatović, Nenad, Uskoković, Dragan, "Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold" in Hemijska industrija, 63, no. 4 (2009):301-307,
https://doi.org/10.2298/HEMIND0904301V .,
https://hdl.handle.net/21.15107/rcub_dais_3362 .