TY  - JOUR
AU  - Lukić, Miodrag J.
AU  - Veselinović, Ljiljana
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Dražić, Goran
AU  - Marković, Smilja
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/306
AB  - Hydroxyapatite nanopowders were prepared in the presence of different concentrations of zirconium ions. Such crystallization conditions yielded significantly reduced particle size and increased specific surface area. Cell viability and oxidative stress studies showed that biocompatibility was not impaired when compared to pure hydroxyapatite. Non-isothermal sintering implied the possibility for suppressing the reaction between hydroxyapatite and zirconia by limiting it to only calcium phosphates. Stress-induced transformation of tetragonal to monoclinic zirconia is facilitated by total hydroxyapatite to β-tricalcium phosphate phase transformation.
PB  - Elsevier
T2  - Materials Letters
T1  - Hydroxyapatite nanopowders prepared in the presence of zirconium ions
SP  - 296
EP  - 300
VL  - 122
DO  - 10.1016/j.matlet.2014.02.072
UR  - https://hdl.handle.net/21.15107/rcub_dais_306
ER  - 

@article{
author = "Lukić, Miodrag J. and Veselinović, Ljiljana and Stevanović, Magdalena and Nunić, Jana and Dražić, Goran and Marković, Smilja and Uskoković, Dragan",
year = "2014",
abstract = "Hydroxyapatite nanopowders were prepared in the presence of different concentrations of zirconium ions. Such crystallization conditions yielded significantly reduced particle size and increased specific surface area. Cell viability and oxidative stress studies showed that biocompatibility was not impaired when compared to pure hydroxyapatite. Non-isothermal sintering implied the possibility for suppressing the reaction between hydroxyapatite and zirconia by limiting it to only calcium phosphates. Stress-induced transformation of tetragonal to monoclinic zirconia is facilitated by total hydroxyapatite to β-tricalcium phosphate phase transformation.",
publisher = "Elsevier",
journal = "Materials Letters",
title = "Hydroxyapatite nanopowders prepared in the presence of zirconium ions",
pages = "296-300",
volume = "122",
doi = "10.1016/j.matlet.2014.02.072",
url = "https://hdl.handle.net/21.15107/rcub_dais_306"
}

Lukić, M. J., Veselinović, L., Stevanović, M., Nunić, J., Dražić, G., Marković, S.,& Uskoković, D.. (2014). Hydroxyapatite nanopowders prepared in the presence of zirconium ions. in Materials Letters
Elsevier., 122, 296-300.
https://doi.org/10.1016/j.matlet.2014.02.072
https://hdl.handle.net/21.15107/rcub_dais_306

Lukić MJ, Veselinović L, Stevanović M, Nunić J, Dražić G, Marković S, Uskoković D. Hydroxyapatite nanopowders prepared in the presence of zirconium ions. in Materials Letters. 2014;122:296-300.
doi:10.1016/j.matlet.2014.02.072
https://hdl.handle.net/21.15107/rcub_dais_306 .

Lukić, Miodrag J., Veselinović, Ljiljana, Stevanović, Magdalena, Nunić, Jana, Dražić, Goran, Marković, Smilja, Uskoković, Dragan, "Hydroxyapatite nanopowders prepared in the presence of zirconium ions" in Materials Letters, 122 (2014):296-300,
https://doi.org/10.1016/j.matlet.2014.02.072 .,
https://hdl.handle.net/21.15107/rcub_dais_306 .

TY  - CONF
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Choi, Jonghoon
AU  - Filipović, Miloš
AU  - Uskoković, Dragan
AU  - Tsotakos, Theodore
AU  - Fragogeorgi, Eirini
AU  - Psimadas, Dimitris
AU  - Palamaris, Lazaros
AU  - Loudos, George
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/584
AB  - Selenium (Se) is an essential trace element with important physiological functions and extensive pharmacological actions. The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials. Uniform, stable, amorphous selenium nanoparticles (SeNps) have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell. Synthesized particles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
T1  - Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality
SP  - 38
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_dais_584
ER  - 

@conference{
author = "Stevanović, Magdalena and Nunić, Jana and Choi, Jonghoon and Filipović, Miloš and Uskoković, Dragan and Tsotakos, Theodore and Fragogeorgi, Eirini and Psimadas, Dimitris and Palamaris, Lazaros and Loudos, George",
year = "2014",
abstract = "Selenium (Se) is an essential trace element with important physiological functions and extensive pharmacological actions. The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials. Uniform, stable, amorphous selenium nanoparticles (SeNps) have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell. Synthesized particles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts",
title = "Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality",
pages = "38-38",
url = "https://hdl.handle.net/21.15107/rcub_dais_584"
}

Stevanović, M., Nunić, J., Choi, J., Filipović, M., Uskoković, D., Tsotakos, T., Fragogeorgi, E., Psimadas, D., Palamaris, L.,& Loudos, G.. (2014). Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 38-38.
https://hdl.handle.net/21.15107/rcub_dais_584

Stevanović M, Nunić J, Choi J, Filipović M, Uskoković D, Tsotakos T, Fragogeorgi E, Psimadas D, Palamaris L, Loudos G. Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:38-38.
https://hdl.handle.net/21.15107/rcub_dais_584 .

Stevanović, Magdalena, Nunić, Jana, Choi, Jonghoon, Filipović, Miloš, Uskoković, Dragan, Tsotakos, Theodore, Fragogeorgi, Eirini, Psimadas, Dimitris, Palamaris, Lazaros, Loudos, George, "Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality" in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts (2014):38-38,
https://hdl.handle.net/21.15107/rcub_dais_584 .

TY  - JOUR
AU  - Stupar, Petar
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/4671
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Paris : Association de pharmacie galenique industrielle
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
SP  - 191
EP  - 197
VL  - 24
IS  - 2
DO  - 10.1016/S1773-2247(14)50031-7
UR  - https://hdl.handle.net/21.15107/rcub_dais_4671
ER  - 

@article{
author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena",
year = "2014",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Paris : Association de pharmacie galenique industrielle",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "191-197",
volume = "24",
number = "2",
doi = "10.1016/S1773-2247(14)50031-7",
url = "https://hdl.handle.net/21.15107/rcub_dais_4671"
}

Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology
Paris : Association de pharmacie galenique industrielle., 24(2), 191-197.
https://doi.org/10.1016/S1773-2247(14)50031-7
https://hdl.handle.net/21.15107/rcub_dais_4671

Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197.
doi:10.1016/S1773-2247(14)50031-7
https://hdl.handle.net/21.15107/rcub_dais_4671 .

Stupar, Petar, Pavlović, Vladimir B., Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Stevanović, Magdalena, "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197,
https://doi.org/10.1016/S1773-2247(14)50031-7 .,
https://hdl.handle.net/21.15107/rcub_dais_4671 .

TY  - JOUR
AU  - Stupar, Petar
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/425
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Paris : Association de pharmacie galenique industrielle
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
SP  - 191
EP  - 197
VL  - 24
IS  - 2
DO  - 10.1016/S1773-2247(14)50031-7
UR  - https://hdl.handle.net/21.15107/rcub_dais_425
ER  - 

@article{
author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena",
year = "2014",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Paris : Association de pharmacie galenique industrielle",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "191-197",
volume = "24",
number = "2",
doi = "10.1016/S1773-2247(14)50031-7",
url = "https://hdl.handle.net/21.15107/rcub_dais_425"
}

Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology
Paris : Association de pharmacie galenique industrielle., 24(2), 191-197.
https://doi.org/10.1016/S1773-2247(14)50031-7
https://hdl.handle.net/21.15107/rcub_dais_425

Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197.
doi:10.1016/S1773-2247(14)50031-7
https://hdl.handle.net/21.15107/rcub_dais_425 .

Stupar, Petar, Pavlović, Vladimir B., Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Stevanović, Magdalena, "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197,
https://doi.org/10.1016/S1773-2247(14)50031-7 .,
https://hdl.handle.net/21.15107/rcub_dais_425 .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Bračko, Ines
AU  - Milenković, Marina
AU  - Filipović, Nenad
AU  - Nunić, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/574
AB  - A water-soluble antioxidant (ascorbic acid, vitamin C) was encapsulated together with poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) within a poly(lactide-co-glycolide) (PLGA) polymeric matrix and their synergistic effects were studied. The PLGA/AgNpPGA/ascorbic acid particles synthesized by a physicochemical method with solvent/non-solvent systems are spherical, have a mean diameter of 775 nm and a narrow size distribution with a polydispersity index of 0.158. The encapsulation efficiency of AgNpPGA/ascorbic acid within PLGA was determined to be >90%. The entire amount of encapsulated ascorbic acid was released in 68 days, and the entire amount of AgNpPGAs was released in 87 days of degradation. The influence of PLGA/AgNpPGA/ascorbic acid on cell viability, generation of reactive oxygen species (ROS) in HepG2 cells, as well as antimicrobial activity against seven different pathogens was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGA/ascorbic acid particles. We measured the kinetics of ROS formation in HepG2 cells by a DCFH-DA assay, and found that PLGA/AgNpPGA/ascorbic acid caused a significant decrease in DCF fluorescence intensity, which was 2-fold lower than that in control cells after a 5 h exposure. This indicates that the PLGA/AgNpPGA/ascorbic acid microspheres either act as scavengers of intracellular ROS and/or reduce their formation. Also, the results of antimicrobial activity of PLGA/AgNpPGA/ascorbic acid obtained by the broth microdilution method showed superior and extended activity of these particles. The samples were characterized using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, zeta potential and particle size analysis. This paper presents a new approach to the treatment of infection that at the same time offers a very pronounced antioxidant effect.
PB  - Elsevier
T2  - Acta Biomaterialia
T1  - Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity
SP  - 151
EP  - 162
VL  - 10
IS  - 1
DO  - 10.1016/j.actbio.2013.08.030
UR  - https://hdl.handle.net/21.15107/rcub_dais_574
ER  - 

@article{
author = "Stevanović, Magdalena and Bračko, Ines and Milenković, Marina and Filipović, Nenad and Nunić, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2014",
abstract = "A water-soluble antioxidant (ascorbic acid, vitamin C) was encapsulated together with poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) within a poly(lactide-co-glycolide) (PLGA) polymeric matrix and their synergistic effects were studied. The PLGA/AgNpPGA/ascorbic acid particles synthesized by a physicochemical method with solvent/non-solvent systems are spherical, have a mean diameter of 775 nm and a narrow size distribution with a polydispersity index of 0.158. The encapsulation efficiency of AgNpPGA/ascorbic acid within PLGA was determined to be >90%. The entire amount of encapsulated ascorbic acid was released in 68 days, and the entire amount of AgNpPGAs was released in 87 days of degradation. The influence of PLGA/AgNpPGA/ascorbic acid on cell viability, generation of reactive oxygen species (ROS) in HepG2 cells, as well as antimicrobial activity against seven different pathogens was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGA/ascorbic acid particles. We measured the kinetics of ROS formation in HepG2 cells by a DCFH-DA assay, and found that PLGA/AgNpPGA/ascorbic acid caused a significant decrease in DCF fluorescence intensity, which was 2-fold lower than that in control cells after a 5 h exposure. This indicates that the PLGA/AgNpPGA/ascorbic acid microspheres either act as scavengers of intracellular ROS and/or reduce their formation. Also, the results of antimicrobial activity of PLGA/AgNpPGA/ascorbic acid obtained by the broth microdilution method showed superior and extended activity of these particles. The samples were characterized using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, zeta potential and particle size analysis. This paper presents a new approach to the treatment of infection that at the same time offers a very pronounced antioxidant effect.",
publisher = "Elsevier",
journal = "Acta Biomaterialia",
title = "Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity",
pages = "151-162",
volume = "10",
number = "1",
doi = "10.1016/j.actbio.2013.08.030",
url = "https://hdl.handle.net/21.15107/rcub_dais_574"
}

Stevanović, M., Bračko, I., Milenković, M., Filipović, N., Nunić, J., Filipič, M.,& Uskoković, D.. (2014). Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity. in Acta Biomaterialia
Elsevier., 10(1), 151-162.
https://doi.org/10.1016/j.actbio.2013.08.030
https://hdl.handle.net/21.15107/rcub_dais_574

Stevanović M, Bračko I, Milenković M, Filipović N, Nunić J, Filipič M, Uskoković D. Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity. in Acta Biomaterialia. 2014;10(1):151-162.
doi:10.1016/j.actbio.2013.08.030
https://hdl.handle.net/21.15107/rcub_dais_574 .

Stevanović, Magdalena, Bračko, Ines, Milenković, Marina, Filipović, Nenad, Nunić, Jana, Filipič, Metka, Uskoković, Dragan, "Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity" in Acta Biomaterialia, 10, no. 1 (2014):151-162,
https://doi.org/10.1016/j.actbio.2013.08.030 .,
https://hdl.handle.net/21.15107/rcub_dais_574 .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/367
AB  - Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.
PB  - Elsevier
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells
SP  - 414
EP  - 424
VL  - 117
IS  - 1
DO  - 10.1016/j.colsurfb.2014.03.015
UR  - https://hdl.handle.net/21.15107/rcub_dais_367
ER  - 

@article{
author = "Filipović, Nenad and Stevanović, Magdalena and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2014",
abstract = "Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.",
publisher = "Elsevier",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells",
pages = "414-424",
volume = "117",
number = "1",
doi = "10.1016/j.colsurfb.2014.03.015",
url = "https://hdl.handle.net/21.15107/rcub_dais_367"
}

Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D.. (2014). Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. in Colloids and Surfaces B: Biointerfaces
Elsevier., 117(1), 414-424.
https://doi.org/10.1016/j.colsurfb.2014.03.015
https://hdl.handle.net/21.15107/rcub_dais_367

Filipović N, Stevanović M, Nunić J, Cundrič S, Filipič M, Uskoković D. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. in Colloids and Surfaces B: Biointerfaces. 2014;117(1):414-424.
doi:10.1016/j.colsurfb.2014.03.015
https://hdl.handle.net/21.15107/rcub_dais_367 .

Filipović, Nenad, Stevanović, Magdalena, Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Uskoković, Dragan, "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells" in Colloids and Surfaces B: Biointerfaces, 117, no. 1 (2014):414-424,
https://doi.org/10.1016/j.colsurfb.2014.03.015 .,
https://hdl.handle.net/21.15107/rcub_dais_367 .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/330
AB  - Nanospheres of poly (ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.
PB  - Elsevier
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells
SP  - 414
EP  - 424
VL  - 117
IS  - 1
DO  - 10.1016/j.colsurfb.2014.03.015
UR  - https://hdl.handle.net/21.15107/rcub_dais_330
ER  - 

@article{
author = "Filipović, Nenad and Stevanović, Magdalena and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2014",
abstract = "Nanospheres of poly (ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.",
publisher = "Elsevier",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells",
pages = "414-424",
volume = "117",
number = "1",
doi = "10.1016/j.colsurfb.2014.03.015",
url = "https://hdl.handle.net/21.15107/rcub_dais_330"
}

Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D.. (2014). Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. in Colloids and Surfaces B: Biointerfaces
Elsevier., 117(1), 414-424.
https://doi.org/10.1016/j.colsurfb.2014.03.015
https://hdl.handle.net/21.15107/rcub_dais_330

Filipović N, Stevanović M, Nunić J, Cundrič S, Filipič M, Uskoković D. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. in Colloids and Surfaces B: Biointerfaces. 2014;117(1):414-424.
doi:10.1016/j.colsurfb.2014.03.015
https://hdl.handle.net/21.15107/rcub_dais_330 .

Filipović, Nenad, Stevanović, Magdalena, Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Uskoković, Dragan, "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells" in Colloids and Surfaces B: Biointerfaces, 117, no. 1 (2014):414-424,
https://doi.org/10.1016/j.colsurfb.2014.03.015 .,
https://hdl.handle.net/21.15107/rcub_dais_330 .

TY  - JOUR
AU  - Lukić, Miodrag J.
AU  - Veselinović, Ljiljana
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Dražić, Goran
AU  - Marković, Smilja
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/307
AB  - Hydroxyapatite nanopowders were prepared in the presence of different concentrations of zirconium ions. Such crystallization conditions yielded significantly reduced particle size and increased specific surface area. Cell viability and oxidative stress studies showed that biocompatibility was not impaired when compared to pure hydroxyapatite. Non-isothermal sintering implied the possibility for suppressing the reaction between hydroxyapatite and zirconia by limiting it to only calcium phosphates. Stress-induced transformation of tetragonal to monoclinic zirconia is facilitated by total hydroxyapatite to β-tricalcium phosphate phase transformation.
PB  - Elsevier
T2  - Materials Letters
T1  - Hydroxyapatite nanopowders prepared in the presence of zirconium ions
SP  - 296
EP  - 300
VL  - 122
DO  - 10.1016/j.matlet.2014.02.072
UR  - https://hdl.handle.net/21.15107/rcub_dais_307
ER  - 

@article{
author = "Lukić, Miodrag J. and Veselinović, Ljiljana and Stevanović, Magdalena and Nunić, Jana and Dražić, Goran and Marković, Smilja and Uskoković, Dragan",
year = "2014",
abstract = "Hydroxyapatite nanopowders were prepared in the presence of different concentrations of zirconium ions. Such crystallization conditions yielded significantly reduced particle size and increased specific surface area. Cell viability and oxidative stress studies showed that biocompatibility was not impaired when compared to pure hydroxyapatite. Non-isothermal sintering implied the possibility for suppressing the reaction between hydroxyapatite and zirconia by limiting it to only calcium phosphates. Stress-induced transformation of tetragonal to monoclinic zirconia is facilitated by total hydroxyapatite to β-tricalcium phosphate phase transformation.",
publisher = "Elsevier",
journal = "Materials Letters",
title = "Hydroxyapatite nanopowders prepared in the presence of zirconium ions",
pages = "296-300",
volume = "122",
doi = "10.1016/j.matlet.2014.02.072",
url = "https://hdl.handle.net/21.15107/rcub_dais_307"
}

Lukić, M. J., Veselinović, L., Stevanović, M., Nunić, J., Dražić, G., Marković, S.,& Uskoković, D.. (2014). Hydroxyapatite nanopowders prepared in the presence of zirconium ions. in Materials Letters
Elsevier., 122, 296-300.
https://doi.org/10.1016/j.matlet.2014.02.072
https://hdl.handle.net/21.15107/rcub_dais_307

Lukić MJ, Veselinović L, Stevanović M, Nunić J, Dražić G, Marković S, Uskoković D. Hydroxyapatite nanopowders prepared in the presence of zirconium ions. in Materials Letters. 2014;122:296-300.
doi:10.1016/j.matlet.2014.02.072
https://hdl.handle.net/21.15107/rcub_dais_307 .

Lukić, Miodrag J., Veselinović, Ljiljana, Stevanović, Magdalena, Nunić, Jana, Dražić, Goran, Marković, Smilja, Uskoković, Dragan, "Hydroxyapatite nanopowders prepared in the presence of zirconium ions" in Materials Letters, 122 (2014):296-300,
https://doi.org/10.1016/j.matlet.2014.02.072 .,
https://hdl.handle.net/21.15107/rcub_dais_307 .

TY  - CONF
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Choi, Jonghoon
AU  - Filipović, Miloš
AU  - Uskoković, Dragan
AU  - Tsotakos, Theodore
AU  - Fragogeorgi, Eirini
AU  - Psimadas, Dimitris
AU  - Palamaris, Lazaros
AU  - Loudos, George
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/855
AB  - The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system.
Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium.
SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanced release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell.
The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.

Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004.

References
1. Popova, N. V. Cancer Lett. 2002, 179, 39–42.
2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038.
3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.
PB  - Athens
C3  - Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013
T1  - In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases
UR  - https://hdl.handle.net/21.15107/rcub_dais_855
ER  - 

@conference{
author = "Stevanović, Magdalena and Nunić, Jana and Choi, Jonghoon and Filipović, Miloš and Uskoković, Dragan and Tsotakos, Theodore and Fragogeorgi, Eirini and Psimadas, Dimitris and Palamaris, Lazaros and Loudos, George",
year = "2013",
abstract = "The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system.
Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium.
SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanced release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell.
The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.

Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004.

References
1. Popova, N. V. Cancer Lett. 2002, 179, 39–42.
2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038.
3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.",
publisher = "Athens",
journal = "Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013",
title = "In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases",
url = "https://hdl.handle.net/21.15107/rcub_dais_855"
}

Stevanović, M., Nunić, J., Choi, J., Filipović, M., Uskoković, D., Tsotakos, T., Fragogeorgi, E., Psimadas, D., Palamaris, L.,& Loudos, G.. (2013). In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases. in Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013
Athens..
https://hdl.handle.net/21.15107/rcub_dais_855

Stevanović M, Nunić J, Choi J, Filipović M, Uskoković D, Tsotakos T, Fragogeorgi E, Psimadas D, Palamaris L, Loudos G. In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases. in Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013. 2013;.
https://hdl.handle.net/21.15107/rcub_dais_855 .

Stevanović, Magdalena, Nunić, Jana, Choi, Jonghoon, Filipović, Miloš, Uskoković, Dragan, Tsotakos, Theodore, Fragogeorgi, Eirini, Psimadas, Dimitris, Palamaris, Lazaros, Loudos, George, "In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases" in Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013 (2013),
https://hdl.handle.net/21.15107/rcub_dais_855 .

TY  - CONF
AU  - Stupar, Petar
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - https://dais.sanu.ac.rs/123456789/531
AB  - A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres
SP  - 104
EP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_dais_531
ER  - 

@conference{
author = "Stupar, Petar and Stevanović, Magdalena and Filipović, Nenad and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2012",
abstract = "A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres",
pages = "104-104",
url = "https://hdl.handle.net/21.15107/rcub_dais_531"
}

Stupar, P., Stevanović, M., Filipović, N., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D.. (2012). Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 104-104.
https://hdl.handle.net/21.15107/rcub_dais_531

Stupar P, Stevanović M, Filipović N, Pavlović VB, Nunić J, Cundrič S, Filipič M, Uskoković D. Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:104-104.
https://hdl.handle.net/21.15107/rcub_dais_531 .

Stupar, Petar, Stevanović, Magdalena, Filipović, Nenad, Pavlović, Vladimir B., Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Uskoković, Dragan, "Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres" in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):104-104,
https://hdl.handle.net/21.15107/rcub_dais_531 .