TY  - JOUR
AU  - Pantović-Pavlović, Marijana R.
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Mirkov, Ivana I.
AU  - Kulaš, Jelena B.
AU  - Malešević, Anastasija Lj.
AU  - Pavlović, Miroslav M.
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14406
AB  - The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants
SP  - 227
VL  - 14
IS  - 4
DO  - 10.3390/jfb14040227
UR  - https://hdl.handle.net/21.15107/rcub_dais_14406
ER  - 

@article{
author = "Pantović-Pavlović, Marijana R. and Ignjatović, Nenad and Panić, Vladimir V. and Mirkov, Ivana I. and Kulaš, Jelena B. and Malešević, Anastasija Lj. and Pavlović, Miroslav M.",
year = "2023",
abstract = "The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants",
pages = "227",
volume = "14",
number = "4",
doi = "10.3390/jfb14040227",
url = "https://hdl.handle.net/21.15107/rcub_dais_14406"
}

Pantović-Pavlović, M. R., Ignjatović, N., Panić, V. V., Mirkov, I. I., Kulaš, J. B., Malešević, A. Lj.,& Pavlović, M. M.. (2023). Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials
Basel : MDPI AG., 14(4), 227.
https://doi.org/10.3390/jfb14040227
https://hdl.handle.net/21.15107/rcub_dais_14406

Pantović-Pavlović MR, Ignjatović N, Panić VV, Mirkov II, Kulaš JB, Malešević AL, Pavlović MM. Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials. 2023;14(4):227.
doi:10.3390/jfb14040227
https://hdl.handle.net/21.15107/rcub_dais_14406 .

Pantović-Pavlović, Marijana R., Ignjatović, Nenad, Panić, Vladimir V., Mirkov, Ivana I., Kulaš, Jelena B., Malešević, Anastasija Lj., Pavlović, Miroslav M., "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants" in Journal of Functional Biomaterials, 14, no. 4 (2023):227,
https://doi.org/10.3390/jfb14040227 .,
https://hdl.handle.net/21.15107/rcub_dais_14406 .