TY  - JOUR
AU  - Degli Esposti, Lorenzo
AU  - Marković, Smilja
AU  - Ignjatović, Nenad
AU  - Panseri, Silvia
AU  - Montesi, Monica
AU  - Adamiano, Alessio
AU  - Fosca, Marco
AU  - Rau, Julietta V.
AU  - Uskoković, Vuk
AU  - Iafisco, Michele
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11639
AB  - Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics
SP  - 4832
EP  - 4845
VL  - 9
IS  - 24
DO  - 10.1039/D1TB00601K
UR  - https://hdl.handle.net/21.15107/rcub_dais_11639
ER  - 

@article{
author = "Degli Esposti, Lorenzo and Marković, Smilja and Ignjatović, Nenad and Panseri, Silvia and Montesi, Monica and Adamiano, Alessio and Fosca, Marco and Rau, Julietta V. and Uskoković, Vuk and Iafisco, Michele",
year = "2021",
abstract = "Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics",
pages = "4832-4845",
volume = "9",
number = "24",
doi = "10.1039/D1TB00601K",
url = "https://hdl.handle.net/21.15107/rcub_dais_11639"
}

Degli Esposti, L., Marković, S., Ignjatović, N., Panseri, S., Montesi, M., Adamiano, A., Fosca, M., Rau, J. V., Uskoković, V.,& Iafisco, M.. (2021). Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B
Royal Society of Chemistry., 9(24), 4832-4845.
https://doi.org/10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11639

Degli Esposti L, Marković S, Ignjatović N, Panseri S, Montesi M, Adamiano A, Fosca M, Rau JV, Uskoković V, Iafisco M. Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B. 2021;9(24):4832-4845.
doi:10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11639 .

Degli Esposti, Lorenzo, Marković, Smilja, Ignjatović, Nenad, Panseri, Silvia, Montesi, Monica, Adamiano, Alessio, Fosca, Marco, Rau, Julietta V., Uskoković, Vuk, Iafisco, Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics" in Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845,
https://doi.org/10.1039/D1TB00601K .,
https://hdl.handle.net/21.15107/rcub_dais_11639 .

TY  - JOUR
AU  - Degli Esposti, Lorenzo
AU  - Marković, Smilja
AU  - Ignjatović, Nenad
AU  - Panseri, Silvia
AU  - Montesi, Monica
AU  - Adamiano, Alessio
AU  - Fosca, Marco
AU  - Rau, Julietta V.
AU  - Uskoković, Vuk
AU  - Iafisco, Michele
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11640
AB  - Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics
SP  - 4832
EP  - 4845
VL  - 9
IS  - 24
DO  - 10.1039/D1TB00601K
UR  - https://hdl.handle.net/21.15107/rcub_dais_11640
ER  - 

@article{
author = "Degli Esposti, Lorenzo and Marković, Smilja and Ignjatović, Nenad and Panseri, Silvia and Montesi, Monica and Adamiano, Alessio and Fosca, Marco and Rau, Julietta V. and Uskoković, Vuk and Iafisco, Michele",
year = "2021",
abstract = "Amorphous calcium phosphate (ACP) is a material of high interest for dentistry, orthopedics, and other biomedical sectors. Being intrinsically metastable, the process of transformation of ACP into a crystalline phase upon heating is of high relevance for the development of innovative bioceramics. Here we have first studied the thermal behavior of a citrate-stabilized ACP (Cit-ACP) also doped with fluoride ions (Cit-FACP) prepared at three different nominal Cit/Ca ratios (i.e. 4, 2, 1) by differential thermal analysis. Next, the physico-chemical features of the crystalline products as well as the in vitro cell response to the materials were investigated. A citrate and fluoride free ACP sample was also tested as the blank. We have found that the activation energy of crystallization of Cit-(F)ACP samples is lower in comparison to the blank ACP and this is influenced by the nominal Cit/Ca molar ratio. Interestingly, we have discovered that the thermal treatment of Cit-(F)ACP at 800 °C yields hydroxyapatite (HA) or fluorapatite (FHA) as the main products differently from blank ACP that, like most of the ACPs reported in the literature, yields β-tricalcium phosphate. This was attributed to the Ca/P ratio of Cit-(F)ACP, which is similar to HA. A study of the crystalline products has revealed that all the (F)HA samples were non-cytotoxic, and retained carbonate ions in the crystal structure despite the heat treatment that should have induced decarbonation. The morphology of the products is influenced by the nominal Cit/Ca ratio and the presence of fluoride, ranging from spherical nanoparticles to micrometric hexagonal rods. Overall, our results prove that the thermal crystallization of Cit-(F)ACP is markedly different from classic ACP based materials and the thermal treatment of Cit-(F)ACP represents an attractive route for producing pure bioactive HA ceramics.",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics",
pages = "4832-4845",
volume = "9",
number = "24",
doi = "10.1039/D1TB00601K",
url = "https://hdl.handle.net/21.15107/rcub_dais_11640"
}

Degli Esposti, L., Marković, S., Ignjatović, N., Panseri, S., Montesi, M., Adamiano, A., Fosca, M., Rau, J. V., Uskoković, V.,& Iafisco, M.. (2021). Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B
Royal Society of Chemistry., 9(24), 4832-4845.
https://doi.org/10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11640

Degli Esposti L, Marković S, Ignjatović N, Panseri S, Montesi M, Adamiano A, Fosca M, Rau JV, Uskoković V, Iafisco M. Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics. in Journal of Materials Chemistry B. 2021;9(24):4832-4845.
doi:10.1039/D1TB00601K
https://hdl.handle.net/21.15107/rcub_dais_11640 .

Degli Esposti, Lorenzo, Marković, Smilja, Ignjatović, Nenad, Panseri, Silvia, Montesi, Monica, Adamiano, Alessio, Fosca, Marco, Rau, Julietta V., Uskoković, Vuk, Iafisco, Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics" in Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845,
https://doi.org/10.1039/D1TB00601K .,
https://hdl.handle.net/21.15107/rcub_dais_11640 .

TY  - DATA
AU  - Degli Esposti, Lorenzo
AU  - Marković, Smilja
AU  - Ignjatović, Nenad
AU  - Panseri, Silvia
AU  - Montesi, Monica
AU  - Adamiano, Alessio
AU  - Fosca, Marco
AU  - Rau, Julietta V.
AU  - Uskoković, Vuk
AU  - Iafisco, Michele
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11642
AB  - Figure S1. The shift in the crystallization peak for (A) Cit-ACP-1 and (B) Cit-FACP-1 to higher temperatures in direct proportion with the heating rate; Figure S2. TGA curves of calcined Cit-ACP-4, Cit-ACP-2, and Cit-ACP-1; Figure S3. Pictures of calcined (A) Cit-ACP-4 and (B) Cit-ACP-1
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K
VL  - 9
IS  - 24
UR  - https://hdl.handle.net/21.15107/rcub_dais_11642
ER  - 

@misc{
author = "Degli Esposti, Lorenzo and Marković, Smilja and Ignjatović, Nenad and Panseri, Silvia and Montesi, Monica and Adamiano, Alessio and Fosca, Marco and Rau, Julietta V. and Uskoković, Vuk and Iafisco, Michele",
year = "2021",
abstract = "Figure S1. The shift in the crystallization peak for (A) Cit-ACP-1 and (B) Cit-FACP-1 to higher temperatures in direct proportion with the heating rate; Figure S2. TGA curves of calcined Cit-ACP-4, Cit-ACP-2, and Cit-ACP-1; Figure S3. Pictures of calcined (A) Cit-ACP-4 and (B) Cit-ACP-1",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K",
volume = "9",
number = "24",
url = "https://hdl.handle.net/21.15107/rcub_dais_11642"
}

Degli Esposti, L., Marković, S., Ignjatović, N., Panseri, S., Montesi, M., Adamiano, A., Fosca, M., Rau, J. V., Uskoković, V.,& Iafisco, M.. (2021). Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K. in Journal of Materials Chemistry B
Royal Society of Chemistry., 9(24).
https://hdl.handle.net/21.15107/rcub_dais_11642

Degli Esposti L, Marković S, Ignjatović N, Panseri S, Montesi M, Adamiano A, Fosca M, Rau JV, Uskoković V, Iafisco M. Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K. in Journal of Materials Chemistry B. 2021;9(24).
https://hdl.handle.net/21.15107/rcub_dais_11642 .

Degli Esposti, Lorenzo, Marković, Smilja, Ignjatović, Nenad, Panseri, Silvia, Montesi, Monica, Adamiano, Alessio, Fosca, Marco, Rau, Julietta V., Uskoković, Vuk, Iafisco, Michele, "Electronic Supplementary Information associated with the article: Degli Esposti Lorenzo, Marković Smilja, Ignjatović Nenad, Panseri Silvia, Montesi Monica, Adamiano Alessio, Fosca Marco, Rau Julietta V., Uskoković Vuk, Iafisco Michele, "Thermal crystallization of amorphous calcium phosphate combined with citrate and fluoride doping: a novel route to produce hydroxyapatite bioceramics." Journal of Materials Chemistry B, 9, no. 24 (2021):4832-4845, https://doi.org/10.1039/D1TB00601K" in Journal of Materials Chemistry B, 9, no. 24 (2021),
https://hdl.handle.net/21.15107/rcub_dais_11642 .