TY  - JOUR
AU  - Ganić, Tea
AU  - Vuletić, Stefana
AU  - Nikolić, Biljana
AU  - Stevanović, Magdalena
AU  - Kuzmanović, Maja
AU  - Kekić, Dušan
AU  - Đurović, Saša
AU  - Cvetković, Stefana
AU  - Mitić-Ćulafić, Dragana
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13509
AB  - Acinetobacter baumannii is an emerging nosocomial pathogen resistant to a wide spectrum of antibiotics, with great potential to form a biofilm, which further aggravates treatment of infections caused by it. Therefore, searching for new potent agents that are efficient against A. baumannii seems to be a necessity. One of them, which has already been proven to possess a wide spectrum of biological activities, including antimicrobial effect, is cinnamon essential oil. Still, further increase of antibacterial efficacy and improvement of bioavailability of cinnamon oil is possible by emulsification process. The aim of this study was comparative analysis of cinnamon essential oil and its emulsion against biofilm forming A. baumannii clinical isolates. Furthermore, the investigation of toxicological aspects of possible applications of essential oil and emulsion was done as well. Gas chromatography–mass spectrometry of essential oil indicated trans-cinnamaldehyde as the most abundant component. The cinnamon emulsion was synthesized from cinnamon essential oil by combining modified low- and high- energy methods. Synthesized emulsion was characterized with Fourier-transform infrared spectroscopy and photon correlation spectroscopy. Both substances exhibited significant antibacterial (minimal inhibitory concentrations in the range 0.125–0.5 mg/ml) and antibiofilm effects (inhibitions of formation and reduction of pre-formed biofilm were 47–81 and 30–62%, respectively). Compared to essential oil, the efficacy of emulsion was even stronger considering the small share of pure oil (20%) in the emulsion. The result of biofilm eradication assay was confirmed by scanning electron microscopy. Even though the cytotoxicity was high especially for the emulsion, genotoxicity was not determined. In conclusion, strong antibacterial/antibiofilm effect against A. baumannii of the cinnamon essential oil and the fact that emulsification even potentiated the activity, seems to be of great significance. Observed cytotoxicity implicated that further analysis is needed in order to clearly determine active principles being responsible for obtained antibacterial/antibiofilm and cytotoxic properties. Copyright © 2022 Ganić, Vuletić, Nikolić, Stevanović, Kuzmanović, Kekić, Đurović, Cvetković and Mitić-Ćulafić.
T2  - Frontiers in Microbiology
T1  - Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii
VL  - 13
DO  - 10.3389/fmicb.2022.989667
UR  - https://hdl.handle.net/21.15107/rcub_dais_13509
ER  - 

@article{
author = "Ganić, Tea and Vuletić, Stefana and Nikolić, Biljana and Stevanović, Magdalena and Kuzmanović, Maja and Kekić, Dušan and Đurović, Saša and Cvetković, Stefana and Mitić-Ćulafić, Dragana",
year = "2022",
abstract = "Acinetobacter baumannii is an emerging nosocomial pathogen resistant to a wide spectrum of antibiotics, with great potential to form a biofilm, which further aggravates treatment of infections caused by it. Therefore, searching for new potent agents that are efficient against A. baumannii seems to be a necessity. One of them, which has already been proven to possess a wide spectrum of biological activities, including antimicrobial effect, is cinnamon essential oil. Still, further increase of antibacterial efficacy and improvement of bioavailability of cinnamon oil is possible by emulsification process. The aim of this study was comparative analysis of cinnamon essential oil and its emulsion against biofilm forming A. baumannii clinical isolates. Furthermore, the investigation of toxicological aspects of possible applications of essential oil and emulsion was done as well. Gas chromatography–mass spectrometry of essential oil indicated trans-cinnamaldehyde as the most abundant component. The cinnamon emulsion was synthesized from cinnamon essential oil by combining modified low- and high- energy methods. Synthesized emulsion was characterized with Fourier-transform infrared spectroscopy and photon correlation spectroscopy. Both substances exhibited significant antibacterial (minimal inhibitory concentrations in the range 0.125–0.5 mg/ml) and antibiofilm effects (inhibitions of formation and reduction of pre-formed biofilm were 47–81 and 30–62%, respectively). Compared to essential oil, the efficacy of emulsion was even stronger considering the small share of pure oil (20%) in the emulsion. The result of biofilm eradication assay was confirmed by scanning electron microscopy. Even though the cytotoxicity was high especially for the emulsion, genotoxicity was not determined. In conclusion, strong antibacterial/antibiofilm effect against A. baumannii of the cinnamon essential oil and the fact that emulsification even potentiated the activity, seems to be of great significance. Observed cytotoxicity implicated that further analysis is needed in order to clearly determine active principles being responsible for obtained antibacterial/antibiofilm and cytotoxic properties. Copyright © 2022 Ganić, Vuletić, Nikolić, Stevanović, Kuzmanović, Kekić, Đurović, Cvetković and Mitić-Ćulafić.",
journal = "Frontiers in Microbiology",
title = "Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii",
volume = "13",
doi = "10.3389/fmicb.2022.989667",
url = "https://hdl.handle.net/21.15107/rcub_dais_13509"
}

Ganić, T., Vuletić, S., Nikolić, B., Stevanović, M., Kuzmanović, M., Kekić, D., Đurović, S., Cvetković, S.,& Mitić-Ćulafić, D.. (2022). Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii. in Frontiers in Microbiology, 13.
https://doi.org/10.3389/fmicb.2022.989667
https://hdl.handle.net/21.15107/rcub_dais_13509

Ganić T, Vuletić S, Nikolić B, Stevanović M, Kuzmanović M, Kekić D, Đurović S, Cvetković S, Mitić-Ćulafić D. Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii. in Frontiers in Microbiology. 2022;13.
doi:10.3389/fmicb.2022.989667
https://hdl.handle.net/21.15107/rcub_dais_13509 .

Ganić, Tea, Vuletić, Stefana, Nikolić, Biljana, Stevanović, Magdalena, Kuzmanović, Maja, Kekić, Dušan, Đurović, Saša, Cvetković, Stefana, Mitić-Ćulafić, Dragana, "Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii" in Frontiers in Microbiology, 13 (2022),
https://doi.org/10.3389/fmicb.2022.989667 .,
https://hdl.handle.net/21.15107/rcub_dais_13509 .

TY  - CONF
AU  - Ganić, Tea
AU  - Vuletić, Stefana
AU  - Stevanović, Magdalena
AU  - Kuzmanović, Maja
AU  - Cvetković, Stefana
AU  - Nikolić, Biljana
AU  - Đurović, Saša
AU  - Kekić, Dušan
AU  - Mitić-Ćulafić, Dragana
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13610
AB  - Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication.
Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon correlation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification.
Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%.
Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii.
PB  - Wroclaw : College of Physiotherapy
C3  - Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022
T1  - Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_dais_13610
ER  - 

@conference{
author = "Ganić, Tea and Vuletić, Stefana and Stevanović, Magdalena and Kuzmanović, Maja and Cvetković, Stefana and Nikolić, Biljana and Đurović, Saša and Kekić, Dušan and Mitić-Ćulafić, Dragana",
year = "2022",
abstract = "Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication.
Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon correlation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification.
Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%.
Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii.",
publisher = "Wroclaw : College of Physiotherapy",
journal = "Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022",
title = "Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_dais_13610"
}

Ganić, T., Vuletić, S., Stevanović, M., Kuzmanović, M., Cvetković, S., Nikolić, B., Đurović, S., Kekić, D.,& Mitić-Ćulafić, D.. (2022). Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates. in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022
Wroclaw : College of Physiotherapy., 85-85.
https://hdl.handle.net/21.15107/rcub_dais_13610

Ganić T, Vuletić S, Stevanović M, Kuzmanović M, Cvetković S, Nikolić B, Đurović S, Kekić D, Mitić-Ćulafić D. Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates. in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022. 2022;:85-85.
https://hdl.handle.net/21.15107/rcub_dais_13610 .

Ganić, Tea, Vuletić, Stefana, Stevanović, Magdalena, Kuzmanović, Maja, Cvetković, Stefana, Nikolić, Biljana, Đurović, Saša, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates" in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022 (2022):85-85,
https://hdl.handle.net/21.15107/rcub_dais_13610 .

TY  - CONF
AU  - Ганић, Теа
AU  - Вулетић, Стефана
AU  - Цветковић, Стефана
AU  - Стевановић, Магдалена
AU  - Кузмановић, Маја
AU  - Ђуровић, Саша
AU  - Николић, Биљана
AU  - Митић-Ћулафић, Драгана
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13611
AB  - Антимикробна резистенција представља велики проблем за јавно здравље због ограничених терапијских могућности. Acinetobacter baumannii је опортунистички патоген, отпоран на многе групе антибиотика и доводи до појаве болничких инфекција. Светска здравствена организација прогласила је A. baumannii за патогенa који ефикасно избегава ефекат конвенционалних антибиотика, тако да је потреба за новим антимикробним средствима евидентна. Природни производи укључујући етарска уља су препозната као добри антимикробни агенси. Цимет је вековима познат зачин који има широку примену и за етарско уље (ЕУ цимета) је показано да поседује различите биолошке активности. Циљ ове студије је било испитивање ефекта ЕУ цимета и његове новосинтетисане наноемулзије (НА) на формирање биофилма A. baumannii. ЕУ цимета је хемијски окарактерисано применом GC/MS анализе. Величина капи и полидисперзитет припремљених НА испитани су фотонском корелационом спектроскопијом. МИК вредности су одређене микродилуционом методом, а антибиофилм активност бојењем кристал виолетом. GC/MS анализа је показала да је најзаступљенија компонента испитиваног ЕУ транс-цинамалдехид (61,9%), док НА показују мултимодалну расподелу величине честица. МИК вредности су се кретале у опсегу 0,25-0,5 mg/mL за EУ и 0,125-0,25 mg/mL за НА. Инхибиција формирања биофилма се кретала до 59% и 69%, за ЕУ и НА. Добијени резултати показују да НА има јачи антимикробни и антибиофилм ефекат, па се може рећи да би емулзификација могла бити приступ ка ефикаснијем сузбијању инфекција А. baumannii.
PB  - Београд : Српско биолошко друштво
C3  - Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
T1  - Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii
SP  - 255
EP  - 255
UR  - https://hdl.handle.net/21.15107/rcub_dais_13611
ER  - 

@conference{
author = "Ганић, Теа and Вулетић, Стефана and Цветковић, Стефана and Стевановић, Магдалена and Кузмановић, Маја and Ђуровић, Саша and Николић, Биљана and Митић-Ћулафић, Драгана",
year = "2022",
abstract = "Антимикробна резистенција представља велики проблем за јавно здравље због ограничених терапијских могућности. Acinetobacter baumannii је опортунистички патоген, отпоран на многе групе антибиотика и доводи до појаве болничких инфекција. Светска здравствена организација прогласила је A. baumannii за патогенa који ефикасно избегава ефекат конвенционалних антибиотика, тако да је потреба за новим антимикробним средствима евидентна. Природни производи укључујући етарска уља су препозната као добри антимикробни агенси. Цимет је вековима познат зачин који има широку примену и за етарско уље (ЕУ цимета) је показано да поседује различите биолошке активности. Циљ ове студије је било испитивање ефекта ЕУ цимета и његове новосинтетисане наноемулзије (НА) на формирање биофилма A. baumannii. ЕУ цимета је хемијски окарактерисано применом GC/MS анализе. Величина капи и полидисперзитет припремљених НА испитани су фотонском корелационом спектроскопијом. МИК вредности су одређене микродилуционом методом, а антибиофилм активност бојењем кристал виолетом. GC/MS анализа је показала да је најзаступљенија компонента испитиваног ЕУ транс-цинамалдехид (61,9%), док НА показују мултимодалну расподелу величине честица. МИК вредности су се кретале у опсегу 0,25-0,5 mg/mL за EУ и 0,125-0,25 mg/mL за НА. Инхибиција формирања биофилма се кретала до 59% и 69%, за ЕУ и НА. Добијени резултати показују да НА има јачи антимикробни и антибиофилм ефекат, па се може рећи да би емулзификација могла бити приступ ка ефикаснијем сузбијању инфекција А. baumannii.",
publisher = "Београд : Српско биолошко друштво",
journal = "Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.",
title = "Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii",
pages = "255-255",
url = "https://hdl.handle.net/21.15107/rcub_dais_13611"
}

Ганић, Т., Вулетић, С., Цветковић, С., Стевановић, М., Кузмановић, М., Ђуровић, С., Николић, Б.,& Митић-Ћулафић, Д.. (2022). Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
Београд : Српско биолошко друштво., 255-255.
https://hdl.handle.net/21.15107/rcub_dais_13611

Ганић Т, Вулетић С, Цветковић С, Стевановић М, Кузмановић М, Ђуровић С, Николић Б, Митић-Ћулафић Д. Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.. 2022;:255-255.
https://hdl.handle.net/21.15107/rcub_dais_13611 .

Ганић, Теа, Вулетић, Стефана, Цветковић, Стефана, Стевановић, Магдалена, Кузмановић, Маја, Ђуровић, Саша, Николић, Биљана, Митић-Ћулафић, Драгана, "Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii" in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022. (2022):255-255,
https://hdl.handle.net/21.15107/rcub_dais_13611 .