TY  - JOUR
AU  - Pantović-Pavlović, Marijana R.
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Mirkov, Ivana I.
AU  - Kulaš, Jelena B.
AU  - Malešević, Anastasija Lj.
AU  - Pavlović, Miroslav M.
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14406
AB  - The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants
SP  - 227
VL  - 14
IS  - 4
DO  - 10.3390/jfb14040227
UR  - https://hdl.handle.net/21.15107/rcub_dais_14406
ER  - 

@article{
author = "Pantović-Pavlović, Marijana R. and Ignjatović, Nenad and Panić, Vladimir V. and Mirkov, Ivana I. and Kulaš, Jelena B. and Malešević, Anastasija Lj. and Pavlović, Miroslav M.",
year = "2023",
abstract = "The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants",
pages = "227",
volume = "14",
number = "4",
doi = "10.3390/jfb14040227",
url = "https://hdl.handle.net/21.15107/rcub_dais_14406"
}

Pantović-Pavlović, M. R., Ignjatović, N., Panić, V. V., Mirkov, I. I., Kulaš, J. B., Malešević, A. Lj.,& Pavlović, M. M.. (2023). Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials
Basel : MDPI AG., 14(4), 227.
https://doi.org/10.3390/jfb14040227
https://hdl.handle.net/21.15107/rcub_dais_14406

Pantović-Pavlović MR, Ignjatović N, Panić VV, Mirkov II, Kulaš JB, Malešević AL, Pavlović MM. Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants. in Journal of Functional Biomaterials. 2023;14(4):227.
doi:10.3390/jfb14040227
https://hdl.handle.net/21.15107/rcub_dais_14406 .

Pantović-Pavlović, Marijana R., Ignjatović, Nenad, Panić, Vladimir V., Mirkov, Ivana I., Kulaš, Jelena B., Malešević, Anastasija Lj., Pavlović, Miroslav M., "Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants" in Journal of Functional Biomaterials, 14, no. 4 (2023):227,
https://doi.org/10.3390/jfb14040227 .,
https://hdl.handle.net/21.15107/rcub_dais_14406 .

TY  - CONF
AU  - Pantović-Pavlović, Marijana
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Pavlović, Miroslav M.
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13652
AB  - This paper deals with the deposition of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium with improved properties by in situ anodization/anaphoretic deposition process. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and post-treatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: FE-SEM, XRD, FTIR, cytotoxicity and in vivo testing.
PB  - Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM
C3  - Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia
T1  - Novel Calcium Phosphate Coatings with Selenum on Titanium
SP  - poster-102
UR  - https://hdl.handle.net/21.15107/rcub_dais_13652
ER  - 

@conference{
author = "Pantović-Pavlović, Marijana and Ignjatović, Nenad and Panić, Vladimir V. and Pavlović, Miroslav M.",
year = "2022",
abstract = "This paper deals with the deposition of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium with improved properties by in situ anodization/anaphoretic deposition process. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and post-treatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: FE-SEM, XRD, FTIR, cytotoxicity and in vivo testing.",
publisher = "Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM",
journal = "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia",
title = "Novel Calcium Phosphate Coatings with Selenum on Titanium",
pages = "poster-102",
url = "https://hdl.handle.net/21.15107/rcub_dais_13652"
}

Pantović-Pavlović, M., Ignjatović, N., Panić, V. V.,& Pavlović, M. M.. (2022). Novel Calcium Phosphate Coatings with Selenum on Titanium. in Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia
Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM., poster-102.
https://hdl.handle.net/21.15107/rcub_dais_13652

Pantović-Pavlović M, Ignjatović N, Panić VV, Pavlović MM. Novel Calcium Phosphate Coatings with Selenum on Titanium. in Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia. 2022;:poster-102.
https://hdl.handle.net/21.15107/rcub_dais_13652 .

Pantović-Pavlović, Marijana, Ignjatović, Nenad, Panić, Vladimir V., Pavlović, Miroslav M., "Novel Calcium Phosphate Coatings with Selenum on Titanium" in Meeting point of the science and practice in the fields of corrosion, materials and environmental protection : proceedings = Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine : knjiga radova / XXIII YuCorr International Conference = XXIII YuCorr Međunarodna konferencija, May 16-19, 2022, Divčibare, Serbia (2022):poster-102,
https://hdl.handle.net/21.15107/rcub_dais_13652 .

TY  - CONF
AU  - Pantović-Pavlović, Marijana
AU  - Ignjatović, Nenad
AU  - Panić, Vladimir V.
AU  - Pavlović, Miroslav M.
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13653
AB  - This paper deals with the issue of defining a new method of anodizing/anaphoretic deposition for the application of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium and anodized titanium coatings with improved properties. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and posttreatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. It primarily deals with the creation and optimization of a new in situ anodizing/anaphoretic deposition process for obtaining multifunctional composite biomaterials. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The aim of the research was to define new in situ anodizing/anaphoretic deposition process and adequate modification of process parameters for application of composite calcium phosphate coatings on titanium and its alloys, wherein innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: AFM, SEM, FE-SEM, roughness testing, XRD, FTIR, bioactivity, biocompatibility, cytotoxicity, antimicrobial activity and in vivo testing.
PB  - Split : Central and EasternEuropeanCommittee for Thermal Analysis and Calorimetry (CEEC-TAC)
C3  - Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1))
T1  - Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_dais_13653
ER  - 

@conference{
author = "Pantović-Pavlović, Marijana and Ignjatović, Nenad and Panić, Vladimir V. and Pavlović, Miroslav M.",
year = "2022",
abstract = "This paper deals with the issue of defining a new method of anodizing/anaphoretic deposition for the application of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium and anodized titanium coatings with improved properties. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and posttreatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. It primarily deals with the creation and optimization of a new in situ anodizing/anaphoretic deposition process for obtaining multifunctional composite biomaterials. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The aim of the research was to define new in situ anodizing/anaphoretic deposition process and adequate modification of process parameters for application of composite calcium phosphate coatings on titanium and its alloys, wherein innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: AFM, SEM, FE-SEM, roughness testing, XRD, FTIR, bioactivity, biocompatibility, cytotoxicity, antimicrobial activity and in vivo testing.",
publisher = "Split : Central and EasternEuropeanCommittee for Thermal Analysis and Calorimetry (CEEC-TAC)",
journal = "Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1))",
title = "Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_dais_13653"
}

Pantović-Pavlović, M., Ignjatović, N., Panić, V. V.,& Pavlović, M. M.. (2022). Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications. in Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1))
Split : Central and EasternEuropeanCommittee for Thermal Analysis and Calorimetry (CEEC-TAC)., 44-44.
https://hdl.handle.net/21.15107/rcub_dais_13653

Pantović-Pavlović M, Ignjatović N, Panić VV, Pavlović MM. Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications. in Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1)). 2022;:44-44.
https://hdl.handle.net/21.15107/rcub_dais_13653 .

Pantović-Pavlović, Marijana, Ignjatović, Nenad, Panić, Vladimir V., Pavlović, Miroslav M., "Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications" in Book of abstracts of the ISt Central and Eastern European Conference on Physical Chemistry and Materials Science (CEEC-PCMS1)) (2022):44-44,
https://hdl.handle.net/21.15107/rcub_dais_13653 .

TY  - JOUR
AU  - Pantović Pavlović, Marijana R.
AU  - Stanojević, Boris P.
AU  - Pavlović, Miroslav M.
AU  - Mihailović, Marija D.
AU  - Stevanović, Jasmina
AU  - Panić, Vladimir V.
AU  - Ignjatović, Nenad
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11739
AB  - The aim of this work was to investigate corrosion resistivity, bioactivity, and antibacterial activity of novel nano-amorphous calcium phosphate (ACP) potentially multifunctional composite coatings with and without chitosan oligosaccharide lactate (ChOL), ACP + ChOL/TiO2 and ACP/TiO2 ACP + ChOL/TiO2, respectively, on the titanium substrate. The coatings were obtained by new single-step in situ anodization of the substrate to generate TiO2 and the anaphoretic electrodeposition process of ACP and ChOL. The obtained coatings were around 300 ± 15 μm thick and consisted of two phases, namely, TiO2 and hybrid composite phases. Both ACP/TiO2 and ACP + ChOL/TiO2 have improved corrosion stability, whereas the ACP + ChOL/TiO2 coating showed better corrosion stability. It was shown that at the very start of the deposition process, the formation of the ChOL/TiO2 layer takes place predominantly, which is followed by the inclusion of ChOL into ACP with simultaneous growth of TiO2. This deposition mechanism resulted in the formation of strongly bonded uniform stable coating with high corrosion resistance. In vitro bioactivity was investigated by immersion of the samples in simulated body fluid (SBF). There is in-bone-like apatite formation on both ACP/TiO2 and ACP + ChOL/TiO2 surfaces upon immersion into SBF, which was proven by X-ray diffraction and Fourier transform infrared spectroscopy. While ACP/TiO2 shows no antibacterial activity, ACP + ChOL/TiO2 samples exhibited three- to fourfold decreases in the number of Staphylococcus aureus and Pseudomonas aeruginosa, respectively, after 420 min. The probable mechanism is binding ChOL with the bacterial cell wall, inhibiting its growth, altering the permeability of the cell membrane, and leading to bacterial death.
PB  - American Chemical Society (ACS)
T2  - ACS Biomaterials Science & Engineering
T1  - Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties
SP  - 3088
EP  - 3102
VL  - 7
IS  - 7
DO  - 10.1021/acsbiomaterials.1c00035
UR  - https://hdl.handle.net/21.15107/rcub_dais_11739
ER  - 

@article{
author = "Pantović Pavlović, Marijana R. and Stanojević, Boris P. and Pavlović, Miroslav M. and Mihailović, Marija D. and Stevanović, Jasmina and Panić, Vladimir V. and Ignjatović, Nenad",
year = "2021",
abstract = "The aim of this work was to investigate corrosion resistivity, bioactivity, and antibacterial activity of novel nano-amorphous calcium phosphate (ACP) potentially multifunctional composite coatings with and without chitosan oligosaccharide lactate (ChOL), ACP + ChOL/TiO2 and ACP/TiO2 ACP + ChOL/TiO2, respectively, on the titanium substrate. The coatings were obtained by new single-step in situ anodization of the substrate to generate TiO2 and the anaphoretic electrodeposition process of ACP and ChOL. The obtained coatings were around 300 ± 15 μm thick and consisted of two phases, namely, TiO2 and hybrid composite phases. Both ACP/TiO2 and ACP + ChOL/TiO2 have improved corrosion stability, whereas the ACP + ChOL/TiO2 coating showed better corrosion stability. It was shown that at the very start of the deposition process, the formation of the ChOL/TiO2 layer takes place predominantly, which is followed by the inclusion of ChOL into ACP with simultaneous growth of TiO2. This deposition mechanism resulted in the formation of strongly bonded uniform stable coating with high corrosion resistance. In vitro bioactivity was investigated by immersion of the samples in simulated body fluid (SBF). There is in-bone-like apatite formation on both ACP/TiO2 and ACP + ChOL/TiO2 surfaces upon immersion into SBF, which was proven by X-ray diffraction and Fourier transform infrared spectroscopy. While ACP/TiO2 shows no antibacterial activity, ACP + ChOL/TiO2 samples exhibited three- to fourfold decreases in the number of Staphylococcus aureus and Pseudomonas aeruginosa, respectively, after 420 min. The probable mechanism is binding ChOL with the bacterial cell wall, inhibiting its growth, altering the permeability of the cell membrane, and leading to bacterial death.",
publisher = "American Chemical Society (ACS)",
journal = "ACS Biomaterials Science & Engineering",
title = "Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties",
pages = "3088-3102",
volume = "7",
number = "7",
doi = "10.1021/acsbiomaterials.1c00035",
url = "https://hdl.handle.net/21.15107/rcub_dais_11739"
}

Pantović Pavlović, M. R., Stanojević, B. P., Pavlović, M. M., Mihailović, M. D., Stevanović, J., Panić, V. V.,& Ignjatović, N.. (2021). Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties. in ACS Biomaterials Science & Engineering
American Chemical Society (ACS)., 7(7), 3088-3102.
https://doi.org/10.1021/acsbiomaterials.1c00035
https://hdl.handle.net/21.15107/rcub_dais_11739

Pantović Pavlović MR, Stanojević BP, Pavlović MM, Mihailović MD, Stevanović J, Panić VV, Ignjatović N. Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties. in ACS Biomaterials Science & Engineering. 2021;7(7):3088-3102.
doi:10.1021/acsbiomaterials.1c00035
https://hdl.handle.net/21.15107/rcub_dais_11739 .

Pantović Pavlović, Marijana R., Stanojević, Boris P., Pavlović, Miroslav M., Mihailović, Marija D., Stevanović, Jasmina, Panić, Vladimir V., Ignjatović, Nenad, "Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties" in ACS Biomaterials Science & Engineering, 7, no. 7 (2021):3088-3102,
https://doi.org/10.1021/acsbiomaterials.1c00035 .,
https://hdl.handle.net/21.15107/rcub_dais_11739 .

TY  - JOUR
AU  - Pantović Pavlović, Marijana R.
AU  - Stanojević, Boris P.
AU  - Pavlović, Miroslav M.
AU  - Mihailović, Marija D.
AU  - Stevanović, Jasmina
AU  - Panić, Vladimir V.
AU  - Ignjatović, Nenad
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11784
AB  - The aim of this work was to investigate corrosion resistivity, bioactivity, and antibacterial activity of novel nano-amorphous calcium phosphate (ACP) potentially multifunctional composite coatings with and without chitosan oligosaccharide lactate (ChOL), ACP + ChOL/TiO2 and ACP/TiO2 ACP + ChOL/TiO2, respectively, on the titanium substrate. The coatings were obtained by new single-step in situ anodization of the substrate to generate TiO2 and the anaphoretic electrodeposition process of ACP and ChOL. The obtained coatings were around 300 ± 15 μm thick and consisted of two phases, namely, TiO2 and hybrid composite phases. Both ACP/TiO2 and ACP + ChOL/TiO2 have improved corrosion stability, whereas the ACP + ChOL/TiO2 coating showed better corrosion stability. It was shown that at the very start of the deposition process, the formation of the ChOL/TiO2 layer takes place predominantly, which is followed by the inclusion of ChOL into ACP with simultaneous growth of TiO2. This deposition mechanism resulted in the formation of strongly bonded uniform stable coating with high corrosion resistance. In vitro bioactivity was investigated by immersion of the samples in simulated body fluid (SBF). There is in-bone-like apatite formation on both ACP/TiO2 and ACP + ChOL/TiO2 surfaces upon immersion into SBF, which was proven by X-ray diffraction and Fourier transform infrared spectroscopy. While ACP/TiO2 shows no antibacterial activity, ACP + ChOL/TiO2 samples exhibited three- to fourfold decreases in the number of Staphylococcus aureus and Pseudomonas aeruginosa, respectively, after 420 min. The probable mechanism is binding ChOL with the bacterial cell wall, inhibiting its growth, altering the permeability of the cell membrane, and leading to bacterial death.
PB  - American Chemical Society (ACS)
T2  - ACS Biomaterials Science & Engineering
T1  - Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties
SP  - 3088
EP  - 3102
VL  - 7
IS  - 7
DO  - 10.1021/acsbiomaterials.1c00035
UR  - https://hdl.handle.net/21.15107/rcub_dais_11784
ER  - 

@article{
author = "Pantović Pavlović, Marijana R. and Stanojević, Boris P. and Pavlović, Miroslav M. and Mihailović, Marija D. and Stevanović, Jasmina and Panić, Vladimir V. and Ignjatović, Nenad",
year = "2021",
abstract = "The aim of this work was to investigate corrosion resistivity, bioactivity, and antibacterial activity of novel nano-amorphous calcium phosphate (ACP) potentially multifunctional composite coatings with and without chitosan oligosaccharide lactate (ChOL), ACP + ChOL/TiO2 and ACP/TiO2 ACP + ChOL/TiO2, respectively, on the titanium substrate. The coatings were obtained by new single-step in situ anodization of the substrate to generate TiO2 and the anaphoretic electrodeposition process of ACP and ChOL. The obtained coatings were around 300 ± 15 μm thick and consisted of two phases, namely, TiO2 and hybrid composite phases. Both ACP/TiO2 and ACP + ChOL/TiO2 have improved corrosion stability, whereas the ACP + ChOL/TiO2 coating showed better corrosion stability. It was shown that at the very start of the deposition process, the formation of the ChOL/TiO2 layer takes place predominantly, which is followed by the inclusion of ChOL into ACP with simultaneous growth of TiO2. This deposition mechanism resulted in the formation of strongly bonded uniform stable coating with high corrosion resistance. In vitro bioactivity was investigated by immersion of the samples in simulated body fluid (SBF). There is in-bone-like apatite formation on both ACP/TiO2 and ACP + ChOL/TiO2 surfaces upon immersion into SBF, which was proven by X-ray diffraction and Fourier transform infrared spectroscopy. While ACP/TiO2 shows no antibacterial activity, ACP + ChOL/TiO2 samples exhibited three- to fourfold decreases in the number of Staphylococcus aureus and Pseudomonas aeruginosa, respectively, after 420 min. The probable mechanism is binding ChOL with the bacterial cell wall, inhibiting its growth, altering the permeability of the cell membrane, and leading to bacterial death.",
publisher = "American Chemical Society (ACS)",
journal = "ACS Biomaterials Science & Engineering",
title = "Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties",
pages = "3088-3102",
volume = "7",
number = "7",
doi = "10.1021/acsbiomaterials.1c00035",
url = "https://hdl.handle.net/21.15107/rcub_dais_11784"
}

Pantović Pavlović, M. R., Stanojević, B. P., Pavlović, M. M., Mihailović, M. D., Stevanović, J., Panić, V. V.,& Ignjatović, N.. (2021). Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties. in ACS Biomaterials Science & Engineering
American Chemical Society (ACS)., 7(7), 3088-3102.
https://doi.org/10.1021/acsbiomaterials.1c00035
https://hdl.handle.net/21.15107/rcub_dais_11784

Pantović Pavlović MR, Stanojević BP, Pavlović MM, Mihailović MD, Stevanović J, Panić VV, Ignjatović N. Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties. in ACS Biomaterials Science & Engineering. 2021;7(7):3088-3102.
doi:10.1021/acsbiomaterials.1c00035
https://hdl.handle.net/21.15107/rcub_dais_11784 .

Pantović Pavlović, Marijana R., Stanojević, Boris P., Pavlović, Miroslav M., Mihailović, Marija D., Stevanović, Jasmina, Panić, Vladimir V., Ignjatović, Nenad, "Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties" in ACS Biomaterials Science & Engineering, 7, no. 7 (2021):3088-3102,
https://doi.org/10.1021/acsbiomaterials.1c00035 .,
https://hdl.handle.net/21.15107/rcub_dais_11784 .

TY  - DATA
AU  - Pantović-Pavlović, Marijana
AU  - Stanojević, Boris
AU  - Pavlović, Miroslav M.
AU  - Mihailović, Marija D.
AU  - Stevanović, Jasmina
AU  - Panić, Vladimir V.
AU  - Ignjatović, Nenad
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11740
AB  - The aim of this work was to investigate corrosion resistivity, bioactivity, and antibacterial activity of novel nano-amorphous calcium phosphate (ACP) potentially multifunctional composite coatings with and without chitosan oligosaccharide lactate (ChOL), ACP + ChOL/TiO2 and ACP/TiO2 ACP + ChOL/TiO2, respectively, on the titanium substrate. Supplementary information: The ANOVA results of antimicrobial activity on S. aureus are shown in Table S1 and the ANOVA results of antimicrobial activity on P. aeruginosa are shown in Table S2. The analysis showed significant difference at the 5% level of confidence.
PB  - American Chemical Society (ACS)
T2  - ACS Biomaterials Science & Engineering
T1  - Supplementary information for the article: Pantović Pavlović, Marijana R., Boris P. Stanojević, Miroslav M. Pavlović, Marija D. Mihailović, Jasmina S. Stevanović, Vladimir V. Panić, and Nenad L. Ignjatović. 2021. “Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties.” ACS Biomaterials Science & Engineering 7 (7): 3088–3102. https://doi.org/10.1021/acsbiomaterials.1c00035
VL  - 7
IS  - 7
UR  - https://hdl.handle.net/21.15107/rcub_dais_11740
ER  - 

@misc{
author = "Pantović-Pavlović, Marijana and Stanojević, Boris and Pavlović, Miroslav M. and Mihailović, Marija D. and Stevanović, Jasmina and Panić, Vladimir V. and Ignjatović, Nenad",
year = "2021",
abstract = "The aim of this work was to investigate corrosion resistivity, bioactivity, and antibacterial activity of novel nano-amorphous calcium phosphate (ACP) potentially multifunctional composite coatings with and without chitosan oligosaccharide lactate (ChOL), ACP + ChOL/TiO2 and ACP/TiO2 ACP + ChOL/TiO2, respectively, on the titanium substrate. Supplementary information: The ANOVA results of antimicrobial activity on S. aureus are shown in Table S1 and the ANOVA results of antimicrobial activity on P. aeruginosa are shown in Table S2. The analysis showed significant difference at the 5% level of confidence.",
publisher = "American Chemical Society (ACS)",
journal = "ACS Biomaterials Science & Engineering",
title = "Supplementary information for the article: Pantović Pavlović, Marijana R., Boris P. Stanojević, Miroslav M. Pavlović, Marija D. Mihailović, Jasmina S. Stevanović, Vladimir V. Panić, and Nenad L. Ignjatović. 2021. “Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties.” ACS Biomaterials Science & Engineering 7 (7): 3088–3102. https://doi.org/10.1021/acsbiomaterials.1c00035",
volume = "7",
number = "7",
url = "https://hdl.handle.net/21.15107/rcub_dais_11740"
}

Pantović-Pavlović, M., Stanojević, B., Pavlović, M. M., Mihailović, M. D., Stevanović, J., Panić, V. V.,& Ignjatović, N.. (2021). Supplementary information for the article: Pantović Pavlović, Marijana R., Boris P. Stanojević, Miroslav M. Pavlović, Marija D. Mihailović, Jasmina S. Stevanović, Vladimir V. Panić, and Nenad L. Ignjatović. 2021. “Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties.” ACS Biomaterials Science & Engineering 7 (7): 3088–3102. https://doi.org/10.1021/acsbiomaterials.1c00035. in ACS Biomaterials Science & Engineering
American Chemical Society (ACS)., 7(7).
https://hdl.handle.net/21.15107/rcub_dais_11740

Pantović-Pavlović M, Stanojević B, Pavlović MM, Mihailović MD, Stevanović J, Panić VV, Ignjatović N. Supplementary information for the article: Pantović Pavlović, Marijana R., Boris P. Stanojević, Miroslav M. Pavlović, Marija D. Mihailović, Jasmina S. Stevanović, Vladimir V. Panić, and Nenad L. Ignjatović. 2021. “Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties.” ACS Biomaterials Science & Engineering 7 (7): 3088–3102. https://doi.org/10.1021/acsbiomaterials.1c00035. in ACS Biomaterials Science & Engineering. 2021;7(7).
https://hdl.handle.net/21.15107/rcub_dais_11740 .

Pantović-Pavlović, Marijana, Stanojević, Boris, Pavlović, Miroslav M., Mihailović, Marija D., Stevanović, Jasmina, Panić, Vladimir V., Ignjatović, Nenad, "Supplementary information for the article: Pantović Pavlović, Marijana R., Boris P. Stanojević, Miroslav M. Pavlović, Marija D. Mihailović, Jasmina S. Stevanović, Vladimir V. Panić, and Nenad L. Ignjatović. 2021. “Anodizing/Anaphoretic Electrodeposition of Nano-Calcium Phosphate/Chitosan Lactate Multifunctional Coatings on Titanium with Advanced Corrosion Resistance, Bioactivity, and Antibacterial Properties.” ACS Biomaterials Science & Engineering 7 (7): 3088–3102. https://doi.org/10.1021/acsbiomaterials.1c00035" in ACS Biomaterials Science & Engineering, 7, no. 7 (2021),
https://hdl.handle.net/21.15107/rcub_dais_11740 .