TY  - JOUR
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Živković, Jelena
AU  - Najdanović, Jelena
AU  - Radenković, Milena
AU  - Vasiljević, Perica
AU  - Ignjatović, Nenad
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14751
AB  - Regenerative medicine (RM) exploits the innate potential of the human body to effectively repair and regenerate damaged tissues and organs with the help of various biomaterials. Tissue engineering (TE) makes it possible to replace damaged tissues and organs with new ones. Research in the field of biomaterials has significantly improved the area of RM and TE. Biomaterials are used as orthopedic, dental, cardiovascular implants, medical devices, in the fields of reconstructive and regenerative medicine, among others. Important preconditions for the biomaterial to be used for implantation are its biocompatibility and biofunctionality. Biomaterials should enable adhesion, migration, proliferation and differentiation of cells. The biological properties of biomaterials are a reflection of their physicochemical properties, such as internal architecture, surface characteristics and charge. Biomaterials used in tissue regeneration should mimic the natural structure of the extracellular matrix and represent a physiological microenvironment for normal cellular functions. These biomaterials should also have adequate biodegradability properties to facilitate the formation and growth of new tissue. Biomaterials for use in RM can be of natural or synthetic origin, polymers by structural properties, ceramic and composite type, and based on bioreactivity they can be bioinert or bioactive. In RM and TE, polymers of different classes, natural and synthetic, are used, which can be made as intelligent materials. The structure of hydrogels in the form of a porous network represents a good matrix for cell activity. Ceramic biomaterials based on hydroxyapatite (HAp) are selected for use in RM and TE, especially solid tissues. Properties, such as composition, particle size, material shape, porosity, surface charge, topography, etc., are relevant for the proper use of HAp materials. The properties of HAp allow modification of its structure, surface, particle size design at the micro and nano level, hybridization with polymers, metals, etc. which is very important for its applications. Designed micro-nanohybrid HAp structure is most similar to the bone structure, making the cell environment closest to natural. Bone tissue engineering (BTE) is based on the combined use of cells, osteostimulating (osteoinductive) factors and biomaterials as a scaffolds and carriers for bone regeneration and defects repair. In BTE adipose-derived mesenchymal stem cells (ADSCs) are often used that are induced in vitro towards osteogenic cells or endothelial cells, and freshly isolated stromal vascular fraction can also be used. Blood components (PRP, blood plasma or blood clot) can be included in the composition of the BTE construct as a source of osteoinductive factors. In vitro models and methods were used to examine the biocompatibility, immunomodulatory and regenerative potential of biomaterials, as well as their influence on cellular functions. After in vitro methods, and before clinical studies, various in vivo animal models are used to examine the regenerative potential of biomaterials, such as subcutaneous implantations and bone defects in tibia, femur and calvaria in experimental animals (mouse, rat, rabbit).
PB  - Banja Luka : Academy of Sciences and Arts of the Republic of Srpska
T2  - Contemporary Materials
T1  - Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective
VL  - 14
IS  - 1
DO  - 10.7251/COMEN2301001N
UR  - https://hdl.handle.net/21.15107/rcub_dais_14751
ER  - 

@article{
author = "Najman, Stevo and Stojanović, Sanja and Živković, Jelena and Najdanović, Jelena and Radenković, Milena and Vasiljević, Perica and Ignjatović, Nenad",
year = "2023",
abstract = "Regenerative medicine (RM) exploits the innate potential of the human body to effectively repair and regenerate damaged tissues and organs with the help of various biomaterials. Tissue engineering (TE) makes it possible to replace damaged tissues and organs with new ones. Research in the field of biomaterials has significantly improved the area of RM and TE. Biomaterials are used as orthopedic, dental, cardiovascular implants, medical devices, in the fields of reconstructive and regenerative medicine, among others. Important preconditions for the biomaterial to be used for implantation are its biocompatibility and biofunctionality. Biomaterials should enable adhesion, migration, proliferation and differentiation of cells. The biological properties of biomaterials are a reflection of their physicochemical properties, such as internal architecture, surface characteristics and charge. Biomaterials used in tissue regeneration should mimic the natural structure of the extracellular matrix and represent a physiological microenvironment for normal cellular functions. These biomaterials should also have adequate biodegradability properties to facilitate the formation and growth of new tissue. Biomaterials for use in RM can be of natural or synthetic origin, polymers by structural properties, ceramic and composite type, and based on bioreactivity they can be bioinert or bioactive. In RM and TE, polymers of different classes, natural and synthetic, are used, which can be made as intelligent materials. The structure of hydrogels in the form of a porous network represents a good matrix for cell activity. Ceramic biomaterials based on hydroxyapatite (HAp) are selected for use in RM and TE, especially solid tissues. Properties, such as composition, particle size, material shape, porosity, surface charge, topography, etc., are relevant for the proper use of HAp materials. The properties of HAp allow modification of its structure, surface, particle size design at the micro and nano level, hybridization with polymers, metals, etc. which is very important for its applications. Designed micro-nanohybrid HAp structure is most similar to the bone structure, making the cell environment closest to natural. Bone tissue engineering (BTE) is based on the combined use of cells, osteostimulating (osteoinductive) factors and biomaterials as a scaffolds and carriers for bone regeneration and defects repair. In BTE adipose-derived mesenchymal stem cells (ADSCs) are often used that are induced in vitro towards osteogenic cells or endothelial cells, and freshly isolated stromal vascular fraction can also be used. Blood components (PRP, blood plasma or blood clot) can be included in the composition of the BTE construct as a source of osteoinductive factors. In vitro models and methods were used to examine the biocompatibility, immunomodulatory and regenerative potential of biomaterials, as well as their influence on cellular functions. After in vitro methods, and before clinical studies, various in vivo animal models are used to examine the regenerative potential of biomaterials, such as subcutaneous implantations and bone defects in tibia, femur and calvaria in experimental animals (mouse, rat, rabbit).",
publisher = "Banja Luka : Academy of Sciences and Arts of the Republic of Srpska",
journal = "Contemporary Materials",
title = "Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective",
volume = "14",
number = "1",
doi = "10.7251/COMEN2301001N",
url = "https://hdl.handle.net/21.15107/rcub_dais_14751"
}

Najman, S., Stojanović, S., Živković, J., Najdanović, J., Radenković, M., Vasiljević, P.,& Ignjatović, N.. (2023). Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective. in Contemporary Materials
Banja Luka : Academy of Sciences and Arts of the Republic of Srpska., 14(1).
https://doi.org/10.7251/COMEN2301001N
https://hdl.handle.net/21.15107/rcub_dais_14751

Najman S, Stojanović S, Živković J, Najdanović J, Radenković M, Vasiljević P, Ignjatović N. Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective. in Contemporary Materials. 2023;14(1).
doi:10.7251/COMEN2301001N
https://hdl.handle.net/21.15107/rcub_dais_14751 .

Najman, Stevo, Stojanović, Sanja, Živković, Jelena, Najdanović, Jelena, Radenković, Milena, Vasiljević, Perica, Ignjatović, Nenad, "Applications of biomaterials in regenerative medicine and tissue engineering – concepts and perspective" in Contemporary Materials, 14, no. 1 (2023),
https://doi.org/10.7251/COMEN2301001N .,
https://hdl.handle.net/21.15107/rcub_dais_14751 .

TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15642
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that are easy to obtain from any kind of discarded teeth and have the ability to differentiate into several cell types which makes them very popular in researches regarding regenerative dentistry. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry and they become very attractive tool for the treatment of bone tissue defects but also as a carriers of bioactive molecules. Before clinical application, thorough preclinical studies should be conducted regarding biocompatibility and biofunctionality of synthesized nanomaterials. Therefore, the aim of this study was to examine the influence of synthesized nanomaterial, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), on the proliferation of DPSCs during osteogenic differentiation in cell culture in vitro. We isolated the cells from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were incubated with two concentrations of CP/PLGA nanoparticles in standard cell culture media and media for osteogenic differentiation. Cell proliferation rate was assessed using MTT and Crystal violet test after one, three and seven days of cell culturing. The results showed time- and concentration-dependent differences in DPSCs proliferation in the presence of nanomaterial. Differences in cell proliferation were noticed between osteogenic and standard cell culture media as well as in results obtained by performed tests. Increased cell proliferation was observed over time in both media but this effect was dependent on nanomaterial concentration. Calcium phosphate-based nanomaterials and DPSCs, based on observed cell materials interactions, could be a promising tool in bone tissue regeneration and further studies using in vitro as well as in vivo models should be conducted to fully unravel all of its potential.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_dais_15642
ER  - 

@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2023",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that are easy to obtain from any kind of discarded teeth and have the ability to differentiate into several cell types which makes them very popular in researches regarding regenerative dentistry. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry and they become very attractive tool for the treatment of bone tissue defects but also as a carriers of bioactive molecules. Before clinical application, thorough preclinical studies should be conducted regarding biocompatibility and biofunctionality of synthesized nanomaterials. Therefore, the aim of this study was to examine the influence of synthesized nanomaterial, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), on the proliferation of DPSCs during osteogenic differentiation in cell culture in vitro. We isolated the cells from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were incubated with two concentrations of CP/PLGA nanoparticles in standard cell culture media and media for osteogenic differentiation. Cell proliferation rate was assessed using MTT and Crystal violet test after one, three and seven days of cell culturing. The results showed time- and concentration-dependent differences in DPSCs proliferation in the presence of nanomaterial. Differences in cell proliferation were noticed between osteogenic and standard cell culture media as well as in results obtained by performed tests. Increased cell proliferation was observed over time in both media but this effect was dependent on nanomaterial concentration. Calcium phosphate-based nanomaterials and DPSCs, based on observed cell materials interactions, could be a promising tool in bone tissue regeneration and further studies using in vitro as well as in vivo models should be conducted to fully unravel all of its potential.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_dais_15642"
}

Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2023). Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 11-11.
https://hdl.handle.net/21.15107/rcub_dais_15642

Tomić M, Stojanović S, Ignjatović N, Najman S. Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:11-11.
https://hdl.handle.net/21.15107/rcub_dais_15642 .

Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):11-11,
https://hdl.handle.net/21.15107/rcub_dais_15642 .

TY  - CONF
AU  - Živković, Jelena M.
AU  - Radenković, Milena
AU  - Stojanović, Sanja
AU  - Najdanović, Jelena
AU  - Cvetković, Vladimir
AU  - Vukelić Nikolić, Marija
AU  - Vučković, Ivica
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2022
UR  - http://informatikaubiomedicini.ekonferencije.com/presentation/paperpresentation/679/6441
UR  - https://dais.sanu.ac.rs/123456789/13655
AB  - Данашњи приступ репарацији и регенерацији ткива често подразумева употребу различитих биоматеријала, самостално или у комбинацији са ћелијама и/или факторима раста. Сви материјали, без обзира да ли су природног порекла или синтетисани, изазивају реакцију околног ткива након имплантације. Ова реакција подразумева низ повезаних догађаја од којих коначно зависи да ли ће имплантирани материјал бити добро прихваћен и обавити своју примарну улогу. Сваки материјал за организам представља страно тело и иницира пролазну инфламаторну реакцију, чија дужина трајања и интензитет умногоме одређују његову даљу судбину. На то утиче хемијски састав материјала, величина и облик његових гранула, порозност, компактност, као и степен биодеградабилности. Тако, судбина материјала може кренути једним од два пута: ка доброј интеграцији са околним ткивом, подршци ћелијама са којима интерагује и, код биодеградабилних материјала, замени здравим, функционалним ткивом, или ће пак изазвати снажан и продужен инфламаторни одговор који резултује потпуном изолацијом материјала од остатка ткива дебелом фиброзном капсулом. In vivo испитивања одговора ткива на имплантирани биоматеријал подразумевају истраживања на различитим анималним моделима и у различитим ткивима, са ортопичним или ектопичним имплантацијама. У овом раду ће бити дат преглед наших in vivo истраживања на различитим моделима имплантација. Користили смо ортопичне моделе за формирање кости код пацова и кунића и моделе супкутаних имплантација различитих биоматеријала мишевима и пацовима. За анализу импланата и околног ткива у временским периодима раног и касног одговора коришћене су бројне методе као што су хистолошка бојења, хистоморфометрија, имунохистохемија, СЕМ, радиографске методе, анализа специфичне експресије гена и друге. In vivo анимални модели у сврху претклиничких испитивања важни су за добијање смерница за клиничку примену.
AB  - Today's approach to tissue repair and regeneration often involves application of different biomaterials, alone or in combination with cells and/or growth factors. All materials, regardless of whether they are of natural origin or synthesized, cause a reaction in the surrounding tissue after implantation. This reaction involves a series of related events on which ultimately depends whether the implanted material will be well accepted and perform its primary role. Each material represents a foreign body for the organism and initiates a transient inflammatory reaction, the duration and intensity of which largely determine materil's further fate. Inflammatory reaction is influenced by material's chemical composition, the size and shape of its granules, porosity, compactness, as well as the degree of material's biodegradability. Thus, the fate of the material can go one of two ways: towards good integration with the surrounding tissue, supporting the cells with which it interacts and, in the case of biodegradable materials, replacement by healthy, functional tissue, or it will cause a strong inflammatory response resulting in the complete isolation of the material from the rest of the tissue with thick fibrous capsule. In vivo research of tissue response to implanted biomaterial involves investigation in different animal models and in different tissues, with orthotopic or ectopic implantations. We used orthotopic models for bone formation in rats and rabbits and models of subcutaneous implantation of various biomaterials in mice and rats. Numerous methods were used for the analysis of implants and surrounding tissue in the time periods of early and late response, such as histological staining, histomorphometry, immunohistochemistry, SEM, radiographic methods, analysis of specific gene expression and others. In vivo animal models for the purpose of preclinical studies are important in order to obtain guidelines for clinical application.
PB  - Banja Luka : ANURS
C3  - Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
T1  - Одговор ткива на различите имплантиране биоматеријале у моделима in vivo
T1  - Tissue response to different implanted biomaterials in models in vivo
UR  - https://hdl.handle.net/21.15107/rcub_dais_13655
ER  - 

@conference{
author = "Živković, Jelena M. and Radenković, Milena and Stojanović, Sanja and Najdanović, Jelena and Cvetković, Vladimir and Vukelić Nikolić, Marija and Vučković, Ivica and Ignjatović, Nenad and Najman, Stevo",
year = "2022",
abstract = "Данашњи приступ репарацији и регенерацији ткива често подразумева употребу различитих биоматеријала, самостално или у комбинацији са ћелијама и/или факторима раста. Сви материјали, без обзира да ли су природног порекла или синтетисани, изазивају реакцију околног ткива након имплантације. Ова реакција подразумева низ повезаних догађаја од којих коначно зависи да ли ће имплантирани материјал бити добро прихваћен и обавити своју примарну улогу. Сваки материјал за организам представља страно тело и иницира пролазну инфламаторну реакцију, чија дужина трајања и интензитет умногоме одређују његову даљу судбину. На то утиче хемијски састав материјала, величина и облик његових гранула, порозност, компактност, као и степен биодеградабилности. Тако, судбина материјала може кренути једним од два пута: ка доброј интеграцији са околним ткивом, подршци ћелијама са којима интерагује и, код биодеградабилних материјала, замени здравим, функционалним ткивом, или ће пак изазвати снажан и продужен инфламаторни одговор који резултује потпуном изолацијом материјала од остатка ткива дебелом фиброзном капсулом. In vivo испитивања одговора ткива на имплантирани биоматеријал подразумевају истраживања на различитим анималним моделима и у различитим ткивима, са ортопичним или ектопичним имплантацијама. У овом раду ће бити дат преглед наших in vivo истраживања на различитим моделима имплантација. Користили смо ортопичне моделе за формирање кости код пацова и кунића и моделе супкутаних имплантација различитих биоматеријала мишевима и пацовима. За анализу импланата и околног ткива у временским периодима раног и касног одговора коришћене су бројне методе као што су хистолошка бојења, хистоморфометрија, имунохистохемија, СЕМ, радиографске методе, анализа специфичне експресије гена и друге. In vivo анимални модели у сврху претклиничких испитивања важни су за добијање смерница за клиничку примену., Today's approach to tissue repair and regeneration often involves application of different biomaterials, alone or in combination with cells and/or growth factors. All materials, regardless of whether they are of natural origin or synthesized, cause a reaction in the surrounding tissue after implantation. This reaction involves a series of related events on which ultimately depends whether the implanted material will be well accepted and perform its primary role. Each material represents a foreign body for the organism and initiates a transient inflammatory reaction, the duration and intensity of which largely determine materil's further fate. Inflammatory reaction is influenced by material's chemical composition, the size and shape of its granules, porosity, compactness, as well as the degree of material's biodegradability. Thus, the fate of the material can go one of two ways: towards good integration with the surrounding tissue, supporting the cells with which it interacts and, in the case of biodegradable materials, replacement by healthy, functional tissue, or it will cause a strong inflammatory response resulting in the complete isolation of the material from the rest of the tissue with thick fibrous capsule. In vivo research of tissue response to implanted biomaterial involves investigation in different animal models and in different tissues, with orthotopic or ectopic implantations. We used orthotopic models for bone formation in rats and rabbits and models of subcutaneous implantation of various biomaterials in mice and rats. Numerous methods were used for the analysis of implants and surrounding tissue in the time periods of early and late response, such as histological staining, histomorphometry, immunohistochemistry, SEM, radiographic methods, analysis of specific gene expression and others. In vivo animal models for the purpose of preclinical studies are important in order to obtain guidelines for clinical application.",
publisher = "Banja Luka : ANURS",
journal = "Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.",
title = "Одговор ткива на различите имплантиране биоматеријале у моделима in vivo, Tissue response to different implanted biomaterials in models in vivo",
url = "https://hdl.handle.net/21.15107/rcub_dais_13655"
}

Živković, J. M., Radenković, M., Stojanović, S., Najdanović, J., Cvetković, V., Vukelić Nikolić, M., Vučković, I., Ignjatović, N.,& Najman, S.. (2022). Одговор ткива на различите имплантиране биоматеријале у моделима in vivo. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
Banja Luka : ANURS..
https://hdl.handle.net/21.15107/rcub_dais_13655

Živković JM, Radenković M, Stojanović S, Najdanović J, Cvetković V, Vukelić Nikolić M, Vučković I, Ignjatović N, Najman S. Одговор ткива на различите имплантиране биоматеријале у моделима in vivo. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.. 2022;.
https://hdl.handle.net/21.15107/rcub_dais_13655 .

Živković, Jelena M., Radenković, Milena, Stojanović, Sanja, Najdanović, Jelena, Cvetković, Vladimir, Vukelić Nikolić, Marija, Vučković, Ivica, Ignjatović, Nenad, Najman, Stevo, "Одговор ткива на различите имплантиране биоматеријале у моделима in vivo" in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022. (2022),
https://hdl.handle.net/21.15107/rcub_dais_13655 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Najdanović, Jelena
AU  - Mitić, Žarko
AU  - Vasiljević, Perica
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2022
UR  - http://informatikaubiomedicini.ekonferencije.com/presentation/paperpresentation/679/6440
UR  - https://dais.sanu.ac.rs/123456789/13654
AB  - Тестирање биоматеријала намењених за примену у регенеративној медицини на ћелијским моделима in vitro је неопходан први корак у циљу процене биокомпатибилности биоматеријала али и њихове биофункционалности. Постоје различити начини тестирања биоматеријала, као и различити ћелијски модели на којима се врши тестирање. Одабир адекватног ћелијског модела врши се на основу врсте биоматеријала и његове потенцијалне примене а тестирање се врши најпре стандардним тестовима као што су тестови цитотоксичности и генотоксичности а затим се испитују друге биолошке карактеристике као што су нпр. способност биоматеријала да индукује диференцијацију ћелија у одређени тип или способност активације макрофага ка одређеном фенотипу и индукција њиховог одговора ин витро, и многе друге. У овом раду ће бити преглед резултата наших досадашњих истраживања у области карактеризације биоматеријала и испитивања на ћелијским моделима in vitro. Испитивали смо биоматеријале намењене за регенерацију тврдих ткива, нпр. материјали на бази хидроксиапатита, калцијум фосфата, природног и синтетског порекла, тако и биоматеријале за регенерацију меких ткива, нпр. материјали на бази колагена или других полимера како природног тако и синтетског порекла. Да би се побољшала биофункционалност биоматеријала често им се додају биоактивне супстанце или њихове комбинације, а материјали могу бити у различитим облицима, у виду скафолда, у виду гранула, нано- или микро- димензија, мембрана и разним другим, при чему облик материјала утиче и на одабир ћелијског модела који се користи за тестирање. За карактеризацију биматеријала користили смо методе као што су SEM, EDX, FTIR и друге. Рађени су и тестови цитотоксичности, генотоксичности, пролиферације, миграције, диференцијације, фагоцитозе и многи други, а биоматеријале смо тестирали у директном контакту са ћелијама и у индиректном контакту. Испитивали смо и промене биоматеријала након интеракције са ћелијама као и понашање биоматеријала у различитим условима in vitro.
AB  - Testing biomaterials intended for use in regenerative medicine on cell models in vitro is a necessary first step in order to assess the biocompatibility of biomaterials as well as their biofunctionality. There are different methods of testing biomaterials, as well as different cell models on which testing is performed. The selection of an adequate cell model is made based on the type of biomaterial and its potential application, and testing is performed first using standard tests such as cytotoxicity and genotoxicity tests, and then other biological characteristics are examined such as, for example, the ability of biomaterials to induce the differentiation of cells into a certain type or the ability to activate macrophages towards a certain phenotype and the induction of their response in vitro, and many others. This paper will present the results of our research in the field of characterization of biomaterials and examination on cell models in vitro. We examined biomaterials intended for the regeneration of hard tissues, e.g. materials based on hydroxyapatite, calcium phosphate, of natural and synthetic origin, as well as biomaterials for the regeneration of soft tissues, e.g. materials based on collagen or other polymers of both natural and synthetic origin. In order to improve the biofunctionality of biomaterials, bioactive substances or their combinations are often added, and the biomaterials can be in different forms, in the form of scaffolds, in the form of granules, nano- or micro-dimensions, membranes and many others, whereby the shape of the material also affects the selection of the cell model used for testing. To characterize bimaterials, we used methods such as SEM, EDX, FTIR and others. In addition to cytotoxicity and genotoxicity tests, we performed proliferation, migration, differentiation, phagocytosis and many other tests, and we tested biomaterials both in direct contact with cells and in indirect contact. We also examined changes in biomaterials after interaction with cells, as well as the behavior of biomaterials in various conditions in vitro.
PB  - Banja Luka : ANURS
C3  - Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
T1  - Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације
T1  - Interaction of cells with different biomaterials in vitro and methods of characterization
UR  - https://hdl.handle.net/21.15107/rcub_dais_13654
ER  - 

@conference{
author = "Stojanović, Sanja and Najdanović, Jelena and Mitić, Žarko and Vasiljević, Perica and Ignjatović, Nenad and Najman, Stevo",
year = "2022",
abstract = "Тестирање биоматеријала намењених за примену у регенеративној медицини на ћелијским моделима in vitro је неопходан први корак у циљу процене биокомпатибилности биоматеријала али и њихове биофункционалности. Постоје различити начини тестирања биоматеријала, као и различити ћелијски модели на којима се врши тестирање. Одабир адекватног ћелијског модела врши се на основу врсте биоматеријала и његове потенцијалне примене а тестирање се врши најпре стандардним тестовима као што су тестови цитотоксичности и генотоксичности а затим се испитују друге биолошке карактеристике као што су нпр. способност биоматеријала да индукује диференцијацију ћелија у одређени тип или способност активације макрофага ка одређеном фенотипу и индукција њиховог одговора ин витро, и многе друге. У овом раду ће бити преглед резултата наших досадашњих истраживања у области карактеризације биоматеријала и испитивања на ћелијским моделима in vitro. Испитивали смо биоматеријале намењене за регенерацију тврдих ткива, нпр. материјали на бази хидроксиапатита, калцијум фосфата, природног и синтетског порекла, тако и биоматеријале за регенерацију меких ткива, нпр. материјали на бази колагена или других полимера како природног тако и синтетског порекла. Да би се побољшала биофункционалност биоматеријала често им се додају биоактивне супстанце или њихове комбинације, а материјали могу бити у различитим облицима, у виду скафолда, у виду гранула, нано- или микро- димензија, мембрана и разним другим, при чему облик материјала утиче и на одабир ћелијског модела који се користи за тестирање. За карактеризацију биматеријала користили смо методе као што су SEM, EDX, FTIR и друге. Рађени су и тестови цитотоксичности, генотоксичности, пролиферације, миграције, диференцијације, фагоцитозе и многи други, а биоматеријале смо тестирали у директном контакту са ћелијама и у индиректном контакту. Испитивали смо и промене биоматеријала након интеракције са ћелијама као и понашање биоматеријала у различитим условима in vitro., Testing biomaterials intended for use in regenerative medicine on cell models in vitro is a necessary first step in order to assess the biocompatibility of biomaterials as well as their biofunctionality. There are different methods of testing biomaterials, as well as different cell models on which testing is performed. The selection of an adequate cell model is made based on the type of biomaterial and its potential application, and testing is performed first using standard tests such as cytotoxicity and genotoxicity tests, and then other biological characteristics are examined such as, for example, the ability of biomaterials to induce the differentiation of cells into a certain type or the ability to activate macrophages towards a certain phenotype and the induction of their response in vitro, and many others. This paper will present the results of our research in the field of characterization of biomaterials and examination on cell models in vitro. We examined biomaterials intended for the regeneration of hard tissues, e.g. materials based on hydroxyapatite, calcium phosphate, of natural and synthetic origin, as well as biomaterials for the regeneration of soft tissues, e.g. materials based on collagen or other polymers of both natural and synthetic origin. In order to improve the biofunctionality of biomaterials, bioactive substances or their combinations are often added, and the biomaterials can be in different forms, in the form of scaffolds, in the form of granules, nano- or micro-dimensions, membranes and many others, whereby the shape of the material also affects the selection of the cell model used for testing. To characterize bimaterials, we used methods such as SEM, EDX, FTIR and others. In addition to cytotoxicity and genotoxicity tests, we performed proliferation, migration, differentiation, phagocytosis and many other tests, and we tested biomaterials both in direct contact with cells and in indirect contact. We also examined changes in biomaterials after interaction with cells, as well as the behavior of biomaterials in various conditions in vitro.",
publisher = "Banja Luka : ANURS",
journal = "Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.",
title = "Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације, Interaction of cells with different biomaterials in vitro and methods of characterization",
url = "https://hdl.handle.net/21.15107/rcub_dais_13654"
}

Stojanović, S., Najdanović, J., Mitić, Ž., Vasiljević, P., Ignjatović, N.,& Najman, S.. (2022). Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.
Banja Luka : ANURS..
https://hdl.handle.net/21.15107/rcub_dais_13654

Stojanović S, Najdanović J, Mitić Ž, Vasiljević P, Ignjatović N, Najman S. Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације. in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022.. 2022;.
https://hdl.handle.net/21.15107/rcub_dais_13654 .

Stojanović, Sanja, Najdanović, Jelena, Mitić, Žarko, Vasiljević, Perica, Ignjatović, Nenad, Najman, Stevo, "Интеракција ћелија са различитим биоматеријалима in vitro и методе карактеризације" in Contemporary Materials 2022 - Савремени материјали, 8-9. 9. 2022. (2022),
https://hdl.handle.net/21.15107/rcub_dais_13654 .

TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/6969
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
T1  - Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro
SP  - 4
EP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_dais_6969
ER  - 

@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2019",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia",
title = "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro",
pages = "4-4",
url = "https://hdl.handle.net/21.15107/rcub_dais_6969"
}

Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2019). Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 4-4.
https://hdl.handle.net/21.15107/rcub_dais_6969

Tomić M, Stojanović S, Ignjatović N, Najman S. Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:4-4.
https://hdl.handle.net/21.15107/rcub_dais_6969 .

Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro" in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia (2019):4-4,
https://hdl.handle.net/21.15107/rcub_dais_6969 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Bajić, Lazar
AU  - Cvetković, Vladimir
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4723
AB  - Nanomaterials represent a relatively new and developing class of materials that are widely used because of their unique mechanical and physiochemical properties. Due to the particles’ size and the ability to easily enter the cells and interact with different cellular components, nanoparticles can exhibit toxic effects in biological systems, so it is necessary to conduct a thorough in vitro biocompatibility testing. Our aim was to examine the in vitro genotoxic and apoptotic potential of two different synthesized nanomaterials intended for bone tissue engineering and regenerative applications: biphasic calcium phosphate coated with poly- D,L-lactide-co-glycolide (CP/PLGA) and cobalt-substituted hydroxyapatite (CoHAp), by DNA fragmentation assay. NB4 cells (acute promyelocytic leukemia cell line) were incubated with different concentrations of CP/PLGA and CoHAp nanomaterials for 24 hours. Cells cultured in standard medium, without nanomaterials, were used as negative control and cells treated with hydrogen peroxide were used as positive control. After incubation with materials the genomic DNA was extracted from the cells and applied on horizontal agarose gel electrophoresis. The results of DNA fragmentation assay were observed on gel documentation system. Higher examined concentrations of CP/PLGA caused DNA fragmentation at some extent with DNA laddering at approximately 180 bp which is characteristic for later stages of apoptosis, while DNA laddering was not seen in the case of CoHAp at the same concentrations. Although both materials are in the form of nanoparticles, a different effect in apoptotic DNA fragmentation was observed which suggests that the chemical composition of nanomaterials notably affects the genotoxicity and apoptosis potential in addition to the particle size.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
T1  - In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_dais_4723
ER  - 

@conference{
author = "Stojanović, Sanja and Bajić, Lazar and Cvetković, Vladimir and Ignjatović, Nenad and Najman, Stevo",
year = "2018",
abstract = "Nanomaterials represent a relatively new and developing class of materials that are widely used because of their unique mechanical and physiochemical properties. Due to the particles’ size and the ability to easily enter the cells and interact with different cellular components, nanoparticles can exhibit toxic effects in biological systems, so it is necessary to conduct a thorough in vitro biocompatibility testing. Our aim was to examine the in vitro genotoxic and apoptotic potential of two different synthesized nanomaterials intended for bone tissue engineering and regenerative applications: biphasic calcium phosphate coated with poly- D,L-lactide-co-glycolide (CP/PLGA) and cobalt-substituted hydroxyapatite (CoHAp), by DNA fragmentation assay. NB4 cells (acute promyelocytic leukemia cell line) were incubated with different concentrations of CP/PLGA and CoHAp nanomaterials for 24 hours. Cells cultured in standard medium, without nanomaterials, were used as negative control and cells treated with hydrogen peroxide were used as positive control. After incubation with materials the genomic DNA was extracted from the cells and applied on horizontal agarose gel electrophoresis. The results of DNA fragmentation assay were observed on gel documentation system. Higher examined concentrations of CP/PLGA caused DNA fragmentation at some extent with DNA laddering at approximately 180 bp which is characteristic for later stages of apoptosis, while DNA laddering was not seen in the case of CoHAp at the same concentrations. Although both materials are in the form of nanoparticles, a different effect in apoptotic DNA fragmentation was observed which suggests that the chemical composition of nanomaterials notably affects the genotoxicity and apoptosis potential in addition to the particle size.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia",
title = "In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_dais_4723"
}

Stojanović, S., Bajić, L., Cvetković, V., Ignjatović, N.,& Najman, S.. (2018). In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 69-69.
https://hdl.handle.net/21.15107/rcub_dais_4723

Stojanović S, Bajić L, Cvetković V, Ignjatović N, Najman S. In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia. 2018;:69-69.
https://hdl.handle.net/21.15107/rcub_dais_4723 .

Stojanović, Sanja, Bajić, Lazar, Cvetković, Vladimir, Ignjatović, Nenad, Najman, Stevo, "In vitro testing of genotoxic and apoptotic potential of two synthesized nanomaterials by DNA fragmentation assay" in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia (2018):69-69,
https://hdl.handle.net/21.15107/rcub_dais_4723 .

TY  - CONF
AU  - Najdanović, Jelena
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Trajanović, Miroslav
PY  - 2017
UR  - https://dais.sanu.ac.rs/123456789/2379
AB  - Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.
PB  - Paris : Atlantis Press
C3  - Proceedings of the IV Advanced Ceramics and Applications Conference
T1  - The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells
SP  - 387
EP  - 400
DO  - 10.2991/978-94-6239-213-7_27
UR  - https://hdl.handle.net/21.15107/rcub_dais_2379
ER  - 

@conference{
editor = "Lee, Bill, Gadow, Rainer, Mitić, Vojislav",
author = "Najdanović, Jelena and Najman, Stevo and Stojanović, Sanja and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Trajanović, Miroslav",
year = "2017",
abstract = "Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.",
publisher = "Paris : Atlantis Press",
journal = "Proceedings of the IV Advanced Ceramics and Applications Conference",
title = "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells",
pages = "387-400",
doi = "10.2991/978-94-6239-213-7_27",
url = "https://hdl.handle.net/21.15107/rcub_dais_2379"
}

Lee, B., Gadow, R., Mitić, V., Najdanović, J., Najman, S., Stojanović, S., Živković, J. M., Ignjatović, N., Uskoković, D.,& Trajanović, M.. (2017). The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference
Paris : Atlantis Press., 387-400.
https://doi.org/10.2991/978-94-6239-213-7_27
https://hdl.handle.net/21.15107/rcub_dais_2379

Lee B, Gadow R, Mitić V, Najdanović J, Najman S, Stojanović S, Živković JM, Ignjatović N, Uskoković D, Trajanović M. The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference. 2017;:387-400.
doi:10.2991/978-94-6239-213-7_27
https://hdl.handle.net/21.15107/rcub_dais_2379 .

Lee, Bill, Gadow, Rainer, Mitić, Vojislav, Najdanović, Jelena, Najman, Stevo, Stojanović, Sanja, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Trajanović, Miroslav, "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells" in Proceedings of the IV Advanced Ceramics and Applications Conference (2017):387-400,
https://doi.org/10.2991/978-94-6239-213-7_27 .,
https://hdl.handle.net/21.15107/rcub_dais_2379 .

TY  - JOUR
AU  - Mitić, Žarko
AU  - Stolić, Aleksandra
AU  - Stojanović, Sanja
AU  - Najman, Stevo
AU  - Ignjatović, Nenad
AU  - Nikolić, Goran
AU  - Trajanović, Miroslav
PY  - 2017
UR  - https://dais.sanu.ac.rs/123456789/2365
AB  - A review of recent advances in instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue is presented in this paper. In recent years, biomaterials attracted great attention primarily because of the wide range of biomedical applications. This paper focuses on the practical aspects of instrumental methods and techniques that were most often applied (X-ray methods, vibrational spectroscopy (IR and Raman), magnetic-resonance spectroscopy (NMR and ESR), mass spectrometry (MS), atomic absorption spectrometry (AAS) and inductively coupled plasma-atomic emission spectrometry (ICP-AES), thermogravimetry (TG), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), scanning electron microscopy (SEM), transmission electron microscopy (TEM)) in the structural investigation and physicochemical characterization of biomaterials and bone tissue. The application of some other physicochemical methods was also discussed. Hands-on information is provided about these valuable research tools, emphasizing practical aspects such as typical measurement conditions, their limitations and advantages, interpretation of results and practical applications.
PB  - Elsevier
T2  - Materials Science and Engineering: C
T1  - Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review
SP  - 930
EP  - 949
VL  - 79
DO  - 10.1016/j.msec.2017.05.127
UR  - https://hdl.handle.net/21.15107/rcub_dais_2365
ER  - 

@article{
author = "Mitić, Žarko and Stolić, Aleksandra and Stojanović, Sanja and Najman, Stevo and Ignjatović, Nenad and Nikolić, Goran and Trajanović, Miroslav",
year = "2017",
abstract = "A review of recent advances in instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue is presented in this paper. In recent years, biomaterials attracted great attention primarily because of the wide range of biomedical applications. This paper focuses on the practical aspects of instrumental methods and techniques that were most often applied (X-ray methods, vibrational spectroscopy (IR and Raman), magnetic-resonance spectroscopy (NMR and ESR), mass spectrometry (MS), atomic absorption spectrometry (AAS) and inductively coupled plasma-atomic emission spectrometry (ICP-AES), thermogravimetry (TG), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), scanning electron microscopy (SEM), transmission electron microscopy (TEM)) in the structural investigation and physicochemical characterization of biomaterials and bone tissue. The application of some other physicochemical methods was also discussed. Hands-on information is provided about these valuable research tools, emphasizing practical aspects such as typical measurement conditions, their limitations and advantages, interpretation of results and practical applications.",
publisher = "Elsevier",
journal = "Materials Science and Engineering: C",
title = "Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review",
pages = "930-949",
volume = "79",
doi = "10.1016/j.msec.2017.05.127",
url = "https://hdl.handle.net/21.15107/rcub_dais_2365"
}

Mitić, Ž., Stolić, A., Stojanović, S., Najman, S., Ignjatović, N., Nikolić, G.,& Trajanović, M.. (2017). Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review. in Materials Science and Engineering: C
Elsevier., 79, 930-949.
https://doi.org/10.1016/j.msec.2017.05.127
https://hdl.handle.net/21.15107/rcub_dais_2365

Mitić Ž, Stolić A, Stojanović S, Najman S, Ignjatović N, Nikolić G, Trajanović M. Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review. in Materials Science and Engineering: C. 2017;79:930-949.
doi:10.1016/j.msec.2017.05.127
https://hdl.handle.net/21.15107/rcub_dais_2365 .

Mitić, Žarko, Stolić, Aleksandra, Stojanović, Sanja, Najman, Stevo, Ignjatović, Nenad, Nikolić, Goran, Trajanović, Miroslav, "Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: A review" in Materials Science and Engineering: C, 79 (2017):930-949,
https://doi.org/10.1016/j.msec.2017.05.127 .,
https://hdl.handle.net/21.15107/rcub_dais_2365 .

TY  - JOUR
AU  - Mitić, Žarko
AU  - Najman, Stevo
AU  - Cakić, Milorad
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Nikolić, Ružica
AU  - Nikolić, Goran
AU  - Stojanović, Sanja
AU  - Vukelić, Marija Đ.
AU  - Trajanović, Miroslav
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/554
AB  - The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period
SP  - 315
EP  - 320
VL  - 1074
DO  - 10.1016/j.molstruc.2014.06.006
UR  - https://hdl.handle.net/21.15107/rcub_dais_554
ER  - 

@article{
author = "Mitić, Žarko and Najman, Stevo and Cakić, Milorad and Ajduković, Zorica and Ignjatović, Nenad and Nikolić, Ružica and Nikolić, Goran and Stojanović, Sanja and Vukelić, Marija Đ. and Trajanović, Miroslav",
year = "2014",
abstract = "The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period",
pages = "315-320",
volume = "1074",
doi = "10.1016/j.molstruc.2014.06.006",
url = "https://hdl.handle.net/21.15107/rcub_dais_554"
}

Mitić, Ž., Najman, S., Cakić, M., Ajduković, Z., Ignjatović, N., Nikolić, R., Nikolić, G., Stojanović, S., Vukelić, M. Đ.,& Trajanović, M.. (2014). Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. in Journal of Molecular Structure
Elsevier., 1074, 315-320.
https://doi.org/10.1016/j.molstruc.2014.06.006
https://hdl.handle.net/21.15107/rcub_dais_554

Mitić Ž, Najman S, Cakić M, Ajduković Z, Ignjatović N, Nikolić R, Nikolić G, Stojanović S, Vukelić MĐ, Trajanović M. Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. in Journal of Molecular Structure. 2014;1074:315-320.
doi:10.1016/j.molstruc.2014.06.006
https://hdl.handle.net/21.15107/rcub_dais_554 .

Mitić, Žarko, Najman, Stevo, Cakić, Milorad, Ajduković, Zorica, Ignjatović, Nenad, Nikolić, Ružica, Nikolić, Goran, Stojanović, Sanja, Vukelić, Marija Đ., Trajanović, Miroslav, "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period" in Journal of Molecular Structure, 1074 (2014):315-320,
https://doi.org/10.1016/j.molstruc.2014.06.006 .,
https://hdl.handle.net/21.15107/rcub_dais_554 .

TY  - CONF
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Mitrić, Žarko
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2012
UR  - https://dais.sanu.ac.rs/123456789/519
AB  - We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_dais_519
ER  - 

@conference{
author = "Najman, Stevo and Stojanović, Sanja and Mitrić, Žarko and Ajduković, Zorica and Ignjatović, Nenad and Uskoković, Dragan",
year = "2012",
abstract = "We have examined the effect of extracts of the calcium phosphate/poly(DL-lactide-co-glycolide) (CP/PLGA) and cobalt-exchanged hydroxyapatite (CoHAp), on the viability of Saos2 osteoblastlike cells. Extracts were prepared by incubation for 3 days at 37°C in the cultivation medium. Conductivity and pH value of extracts were measured before viability assay. Cell viability was estimated by MTT test performed after 24 h incubation of cells with various concentrations of extracts. Extract of CP/PLGA acted more cytotoxic on osteoblasts than the extract of CoHAp. This difference in the effect of examined extracts can be explained by their different pH value and conductivity.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_dais_519"
}

Najman, S., Stojanović, S., Mitrić, Ž., Ajduković, Z., Ignjatović, N.,& Uskoković, D.. (2012). Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., 110-110.
https://hdl.handle.net/21.15107/rcub_dais_519

Najman S, Stojanović S, Mitrić Ž, Ajduković Z, Ignjatović N, Uskoković D. Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells. in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:110-110.
https://hdl.handle.net/21.15107/rcub_dais_519 .

Najman, Stevo, Stojanović, Sanja, Mitrić, Žarko, Ajduković, Zorica, Ignjatović, Nenad, Uskoković, Dragan, "Examination of the influence of nanomaterials calcium phosphate/poly(DL-lactide-co-glycolide) and cobalt-exchanged hydroxyapatite on the viability of Saos-2 cells" in Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts (2012):110-110,
https://hdl.handle.net/21.15107/rcub_dais_519 .

TY  - CONF
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Najdanović, Jelena
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2011
UR  - https://dais.sanu.ac.rs/123456789/698
AB  - The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
T1  - Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines
SP  - 6
EP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_dais_698
ER  - 

@conference{
author = "Najman, Stevo and Stojanović, Sanja and Najdanović, Jelena and Ignjatović, Nenad and Uskoković, Dragan",
year = "2011",
abstract = "The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia",
title = "Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines",
pages = "6-6",
url = "https://hdl.handle.net/21.15107/rcub_dais_698"
}

Najman, S., Stojanović, S., Najdanović, J., Ignjatović, N.,& Uskoković, D.. (2011). Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 6-6.
https://hdl.handle.net/21.15107/rcub_dais_698

Najman S, Stojanović S, Najdanović J, Ignjatović N, Uskoković D. Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:6-6.
https://hdl.handle.net/21.15107/rcub_dais_698 .

Najman, Stevo, Stojanović, Sanja, Najdanović, Jelena, Ignjatović, Nenad, Uskoković, Dragan, "Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines" in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):6-6,
https://hdl.handle.net/21.15107/rcub_dais_698 .