TY  - JOUR
AU  - Guševac Stojanović, Ivana
AU  - Drakulić, Dunja
AU  - Todorović, Ana
AU  - Martinović, Jelena
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16514
AB  - While the effects of chronic exposure to microplastic particles (MPs) are extensively studied, the outcomes of a single treatment have received relatively less attention. To investigate MPs’ potential acute toxicity, including their impact on general health status (victual consumption, sensorimotor deficits, and clinical toxicity signs) and serum biochemical parameters (markers of organ/tissue function and oxidative stress indicators), we administered thoroughly characterized MPs (1.4, 35, or 125 mg/kg), generated from polyethylene terephthalate (PET) bottles, to adult male Wistar rats via oral gavage. The MPs’ short-term effects were assessed with well-established tests and methods. The results point to the absence of sensorimotor deficits and clinical toxicity signs, while levels of markers of liver, heart, and kidney function were altered in all MP groups. Decreased victual consumption and increased levels of oxidative stress indicators were evident following treatment with the two higher MP doses. Presented data indicate that examined MPs are able to initiate the development of local changes in tissues and organs within a short time frame, potentially leading to their damage and dysfunction. This study may increase the awareness of the detrimental effects of plastic contamination, as even a single exposure to MPs may provoke adverse health outcomes.
PB  - Basel : MDPI AG
T2  - Toxics
T1  - Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats
SP  - 167
VL  - 12
IS  - 3
DO  - 10.3390/toxics12030167
UR  - https://hdl.handle.net/21.15107/rcub_dais_16514
ER  - 

@article{
author = "Guševac Stojanović, Ivana and Drakulić, Dunja and Todorović, Ana and Martinović, Jelena and Filipović, Nenad and Stojanović, Zoran",
year = "2024",
abstract = "While the effects of chronic exposure to microplastic particles (MPs) are extensively studied, the outcomes of a single treatment have received relatively less attention. To investigate MPs’ potential acute toxicity, including their impact on general health status (victual consumption, sensorimotor deficits, and clinical toxicity signs) and serum biochemical parameters (markers of organ/tissue function and oxidative stress indicators), we administered thoroughly characterized MPs (1.4, 35, or 125 mg/kg), generated from polyethylene terephthalate (PET) bottles, to adult male Wistar rats via oral gavage. The MPs’ short-term effects were assessed with well-established tests and methods. The results point to the absence of sensorimotor deficits and clinical toxicity signs, while levels of markers of liver, heart, and kidney function were altered in all MP groups. Decreased victual consumption and increased levels of oxidative stress indicators were evident following treatment with the two higher MP doses. Presented data indicate that examined MPs are able to initiate the development of local changes in tissues and organs within a short time frame, potentially leading to their damage and dysfunction. This study may increase the awareness of the detrimental effects of plastic contamination, as even a single exposure to MPs may provoke adverse health outcomes.",
publisher = "Basel : MDPI AG",
journal = "Toxics",
title = "Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats",
pages = "167",
volume = "12",
number = "3",
doi = "10.3390/toxics12030167",
url = "https://hdl.handle.net/21.15107/rcub_dais_16514"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Martinović, J., Filipović, N.,& Stojanović, Z.. (2024). Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats. in Toxics
Basel : MDPI AG., 12(3), 167.
https://doi.org/10.3390/toxics12030167
https://hdl.handle.net/21.15107/rcub_dais_16514

Guševac Stojanović I, Drakulić D, Todorović A, Martinović J, Filipović N, Stojanović Z. Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats. in Toxics. 2024;12(3):167.
doi:10.3390/toxics12030167
https://hdl.handle.net/21.15107/rcub_dais_16514 .

Guševac Stojanović, Ivana, Drakulić, Dunja, Todorović, Ana, Martinović, Jelena, Filipović, Nenad, Stojanović, Zoran, "Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats" in Toxics, 12, no. 3 (2024):167,
https://doi.org/10.3390/toxics12030167 .,
https://hdl.handle.net/21.15107/rcub_dais_16514 .

TY  - CONF
AU  - Tomić, Nina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16245
AB  - Kontinuirano interesovanje za nanočestice različitih materijala i njihove kompozite stvorilo je brojna pitanja u vezi njihove potencijalne toksičnosti. Polifenol resveratrol i jedinjenja sa selenom su među agensima čijoj je upotrebi nanotehnologija omogućila nov pristup. U formi nanočestica, oni pokazuju poboljšanu biokompatibilnost i biološke efekte, kao što je antimikrobna aktivnost. Na osnovu podataka iz literature, nanočestice selena su najčešće manje toksične nego jedinjenja sa selenom. U našem istraživanju, najpre smo pripremili kompozitni materijal na bazi selena i resveratrola. Nakon toga, kompozit i njegove komponente bili su testirani pomoću MTT tetrazolijumske boje za utvrđivanje citotoksičnosti, komet testom na genotoksičnost i nitroplavo-tetrazolijumskim testom za procenu nivoa superoksidnih anjona. U svim eksperimentima je korišćena zdrava ćelijska linija fibroblasta čoveka (MRC-5). Rezultati su pokazali da, iako čestice resveratrola ispoljavaju citotoksičnost u koncentracijama preko 12 μg/ml, one nisu genotoksične i ne indukuju stvaranje superoksidnih anjona. Sa druge strane, nanočestice selena nisu bile citotoksične ispod 150 μg/ml, ali su njima tretirane ćelije pokazale znake oštećenja DNK i povećanje produkcije superoksidnih anjona, čak i na 18 μg/ml. Kompozit je pokazao drugačije efekte, indukujući višu citotoksičnost i oksidativni stres nego ekvivalentne koncentracije komponenti, ali istovremeno nije bio genotoksičan. Moguće je da su interakcije nanočestica selena sa nanočesticama resveratrola dovele do pojave jedinstvenih osobina kompozita, potvrđujući izuzetnu važnost testiranja nanokompozitnih materijala uporedo sa njihovim komponentama.
AB  - The continuous interest in engineered nanoparticles of various materials, and their composites, has raised numerous questions regarding their toxicological profiles. Polyphenol resveratrol and selenium compounds are among the agents to which nanotechnology has given all new approach. Their particulate forms possess improved bioavailability and biological effects, such as antibacterial activity. Furthermore, selenium nanoparticles usually have significantly lower toxicity compared to selenium compounds. In this study, we prepared composite material consisting of selenium and resveratrol nanoparticles. Then, composite material and its components were assessed by using tetrazolium MTT dye for determining cytotoxicity, comet assay for genotoxicity study, and nitroblue tetrazolium assay (NBT) assay to estimate levels of superoxide anions. The normal human fibroblast cells (MRC-5) were used in all the experiments. Results showed that, although the resveratrol component exhibited cytotoxicity at 12 μg/ml, it was not genotoxic, and did not induce superoxide anion production. Selenium nanoparticles, on the other hand, were not cytotoxic below 150 μg/ml, but treated cells showed signs of DNA damage and elevation in superoxide anion production even at 18 μg/ml. Composite material behaved differently, inducing higher cytotoxicity and oxidative stress than comparable concentrations of components, but also showed lack of genotoxicity. Possible interactions of selenium nanoparticles with resveratrol nanoparticles could have created unique composite properties, confirming the great importance of toxicity testing of nanocomposite materials along with their components.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
T1  - Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona
T1  - Cytotoxic and genotoxic potential of composite based on resveratrol and selenium nanoparticles, and its potential for superoxide anion induction
SP  - 193
EP  - 194
UR  - https://hdl.handle.net/21.15107/rcub_dais_16245
ER  - 

@conference{
author = "Tomić, Nina and Stevanović, Magdalena and Filipović, Nenad and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Kontinuirano interesovanje za nanočestice različitih materijala i njihove kompozite stvorilo je brojna pitanja u vezi njihove potencijalne toksičnosti. Polifenol resveratrol i jedinjenja sa selenom su među agensima čijoj je upotrebi nanotehnologija omogućila nov pristup. U formi nanočestica, oni pokazuju poboljšanu biokompatibilnost i biološke efekte, kao što je antimikrobna aktivnost. Na osnovu podataka iz literature, nanočestice selena su najčešće manje toksične nego jedinjenja sa selenom. U našem istraživanju, najpre smo pripremili kompozitni materijal na bazi selena i resveratrola. Nakon toga, kompozit i njegove komponente bili su testirani pomoću MTT tetrazolijumske boje za utvrđivanje citotoksičnosti, komet testom na genotoksičnost i nitroplavo-tetrazolijumskim testom za procenu nivoa superoksidnih anjona. U svim eksperimentima je korišćena zdrava ćelijska linija fibroblasta čoveka (MRC-5). Rezultati su pokazali da, iako čestice resveratrola ispoljavaju citotoksičnost u koncentracijama preko 12 μg/ml, one nisu genotoksične i ne indukuju stvaranje superoksidnih anjona. Sa druge strane, nanočestice selena nisu bile citotoksične ispod 150 μg/ml, ali su njima tretirane ćelije pokazale znake oštećenja DNK i povećanje produkcije superoksidnih anjona, čak i na 18 μg/ml. Kompozit je pokazao drugačije efekte, indukujući višu citotoksičnost i oksidativni stres nego ekvivalentne koncentracije komponenti, ali istovremeno nije bio genotoksičan. Moguće je da su interakcije nanočestica selena sa nanočesticama resveratrola dovele do pojave jedinstvenih osobina kompozita, potvrđujući izuzetnu važnost testiranja nanokompozitnih materijala uporedo sa njihovim komponentama., The continuous interest in engineered nanoparticles of various materials, and their composites, has raised numerous questions regarding their toxicological profiles. Polyphenol resveratrol and selenium compounds are among the agents to which nanotechnology has given all new approach. Their particulate forms possess improved bioavailability and biological effects, such as antibacterial activity. Furthermore, selenium nanoparticles usually have significantly lower toxicity compared to selenium compounds. In this study, we prepared composite material consisting of selenium and resveratrol nanoparticles. Then, composite material and its components were assessed by using tetrazolium MTT dye for determining cytotoxicity, comet assay for genotoxicity study, and nitroblue tetrazolium assay (NBT) assay to estimate levels of superoxide anions. The normal human fibroblast cells (MRC-5) were used in all the experiments. Results showed that, although the resveratrol component exhibited cytotoxicity at 12 μg/ml, it was not genotoxic, and did not induce superoxide anion production. Selenium nanoparticles, on the other hand, were not cytotoxic below 150 μg/ml, but treated cells showed signs of DNA damage and elevation in superoxide anion production even at 18 μg/ml. Composite material behaved differently, inducing higher cytotoxicity and oxidative stress than comparable concentrations of components, but also showed lack of genotoxicity. Possible interactions of selenium nanoparticles with resveratrol nanoparticles could have created unique composite properties, confirming the great importance of toxicity testing of nanocomposite materials along with their components.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book",
title = "Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona, Cytotoxic and genotoxic potential of composite based on resveratrol and selenium nanoparticles, and its potential for superoxide anion induction",
pages = "193-194",
url = "https://hdl.handle.net/21.15107/rcub_dais_16245"
}

Tomić, N., Stevanović, M., Filipović, N.,& Mitić Ćulafić, D.. (2023). Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
Beograd : Udruženje toksikologa Srbije., 193-194.
https://hdl.handle.net/21.15107/rcub_dais_16245

Tomić N, Stevanović M, Filipović N, Mitić Ćulafić D. Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book. 2023;:193-194.
https://hdl.handle.net/21.15107/rcub_dais_16245 .

Tomić, Nina, Stevanović, Magdalena, Filipović, Nenad, Mitić Ćulafić, Dragana, "Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book (2023):193-194,
https://hdl.handle.net/21.15107/rcub_dais_16245 .

TY  - JOUR
AU  - Mitrović Rajić, Anđela
AU  - Pantić, Tijana
AU  - Milošević-Govedarović, Sanja
AU  - Paskaš Mamula, Bojana
AU  - Filipović, Nenad
AU  - Grbović Novaković, Jasmina
AU  - Dimitrijević, Silvana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16244
AB  - The effect of mechanical milling on the thermal behavior of pyrophyllite ore from a deposit in Parsovići, Bosnia and Herzegovina, was characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and Particle size distribution. The thermal behavior of the material was followed by thermogravimetric and differential thermal analysis and correlated to its microstructural properties. The Williamson-Hall model was used to calculate the crystallite size and microstrain. Mechanochemical treatment of pyrophyllite ore produced a substantial structural modification, mainly along the c axis, resulting in disorder and partial degradation of the crystal structure of the ore. The particle size diminution, induced defects, and microstrain in the crystal lattice cause decrease in the peak intensity until the final disappearance. As confirmed by scanning electron microscopy and particle-size-distribution analysis, the surface area and the agglomeration is more pronounced as grinding time increases. Dehydroxylation of the minerals in the unmilled ore was realized at 716oC confirm by FTIR analysis. The endothermic peak that corresponds to dehydroxylation is shifted toward lower temperatures and becomes broad giving rise to the formation of amorphous SiO2 as milling time increases.
PB  - Belgrade : ETRAN
T2  - Science of Sintering
T1  - Influence of mechanochemical activation on the thermal behavior of pyrophyllite
SP  - 453
EP  - 467
VL  - 55
IS  - 4
DO  - 10.2298/SOS220715018M
UR  - https://hdl.handle.net/21.15107/rcub_dais_16244
ER  - 

@article{
author = "Mitrović Rajić, Anđela and Pantić, Tijana and Milošević-Govedarović, Sanja and Paskaš Mamula, Bojana and Filipović, Nenad and Grbović Novaković, Jasmina and Dimitrijević, Silvana",
year = "2023",
abstract = "The effect of mechanical milling on the thermal behavior of pyrophyllite ore from a deposit in Parsovići, Bosnia and Herzegovina, was characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and Particle size distribution. The thermal behavior of the material was followed by thermogravimetric and differential thermal analysis and correlated to its microstructural properties. The Williamson-Hall model was used to calculate the crystallite size and microstrain. Mechanochemical treatment of pyrophyllite ore produced a substantial structural modification, mainly along the c axis, resulting in disorder and partial degradation of the crystal structure of the ore. The particle size diminution, induced defects, and microstrain in the crystal lattice cause decrease in the peak intensity until the final disappearance. As confirmed by scanning electron microscopy and particle-size-distribution analysis, the surface area and the agglomeration is more pronounced as grinding time increases. Dehydroxylation of the minerals in the unmilled ore was realized at 716oC confirm by FTIR analysis. The endothermic peak that corresponds to dehydroxylation is shifted toward lower temperatures and becomes broad giving rise to the formation of amorphous SiO2 as milling time increases.",
publisher = "Belgrade : ETRAN",
journal = "Science of Sintering",
title = "Influence of mechanochemical activation on the thermal behavior of pyrophyllite",
pages = "453-467",
volume = "55",
number = "4",
doi = "10.2298/SOS220715018M",
url = "https://hdl.handle.net/21.15107/rcub_dais_16244"
}

Mitrović Rajić, A., Pantić, T., Milošević-Govedarović, S., Paskaš Mamula, B., Filipović, N., Grbović Novaković, J.,& Dimitrijević, S.. (2023). Influence of mechanochemical activation on the thermal behavior of pyrophyllite. in Science of Sintering
Belgrade : ETRAN., 55(4), 453-467.
https://doi.org/10.2298/SOS220715018M
https://hdl.handle.net/21.15107/rcub_dais_16244

Mitrović Rajić A, Pantić T, Milošević-Govedarović S, Paskaš Mamula B, Filipović N, Grbović Novaković J, Dimitrijević S. Influence of mechanochemical activation on the thermal behavior of pyrophyllite. in Science of Sintering. 2023;55(4):453-467.
doi:10.2298/SOS220715018M
https://hdl.handle.net/21.15107/rcub_dais_16244 .

Mitrović Rajić, Anđela, Pantić, Tijana, Milošević-Govedarović, Sanja, Paskaš Mamula, Bojana, Filipović, Nenad, Grbović Novaković, Jasmina, Dimitrijević, Silvana, "Influence of mechanochemical activation on the thermal behavior of pyrophyllite" in Science of Sintering, 55, no. 4 (2023):453-467,
https://doi.org/10.2298/SOS220715018M .,
https://hdl.handle.net/21.15107/rcub_dais_16244 .

TY  - JOUR
AU  - Sekulić, Zorana
AU  - Grbović Novaković, Jasmina
AU  - Babić, Bojana
AU  - Prvulović, Milica
AU  - Milanović, Igor
AU  - Novaković, Nikola
AU  - Rajnović, Dragan
AU  - Filipović, Nenad
AU  - Asanović, Vanja
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15140
AB  - The effects of catalysis using vanadium as an additive (2 and 5 wt.%) in a high-energy ball mill on composite desorption properties were examined. The influence of microstructure on the dehydration temperature and hydrogen desorption kinetics was monitored. Morphological and microstructural studies of the synthesized sample were performed by X-ray diffraction (XRD), laser particle size distribution (PSD), and scanning electron microscopy (SEM) methods, while differential scanning calorimetry (DSC) determined thermal properties. To further access amorph species in the milling blend, the absorption spectra were obtained by FTIR-ATR analysis (Fourier transform infrared spectroscopy attenuated total reflection). The results show lower apparent activation energy (Eapp) and H2 desorption temperature are obtained for milling bland with 5 wt.% added vanadium. The best explanation of hydrogen desorption reaction shows the Avrami-Erofeev model for parameter n = 4. Since the obtained value of apparent activation energy is close to the Mg-H bond-breaking energy, one can conclude that breaking this bond would be the rate-limiting step of the process.
T2  - Materials
T1  - The Catalytic Effect of Vanadium on Sorption Properties of MgH2-Based Nanocomposites Obtained Using Low Milling Time
SP  - 5480
VL  - 16
IS  - 15
DO  - 10.3390/ma16155480
UR  - https://hdl.handle.net/21.15107/rcub_dais_15140
ER  - 

@article{
author = "Sekulić, Zorana and Grbović Novaković, Jasmina and Babić, Bojana and Prvulović, Milica and Milanović, Igor and Novaković, Nikola and Rajnović, Dragan and Filipović, Nenad and Asanović, Vanja",
year = "2023",
abstract = "The effects of catalysis using vanadium as an additive (2 and 5 wt.%) in a high-energy ball mill on composite desorption properties were examined. The influence of microstructure on the dehydration temperature and hydrogen desorption kinetics was monitored. Morphological and microstructural studies of the synthesized sample were performed by X-ray diffraction (XRD), laser particle size distribution (PSD), and scanning electron microscopy (SEM) methods, while differential scanning calorimetry (DSC) determined thermal properties. To further access amorph species in the milling blend, the absorption spectra were obtained by FTIR-ATR analysis (Fourier transform infrared spectroscopy attenuated total reflection). The results show lower apparent activation energy (Eapp) and H2 desorption temperature are obtained for milling bland with 5 wt.% added vanadium. The best explanation of hydrogen desorption reaction shows the Avrami-Erofeev model for parameter n = 4. Since the obtained value of apparent activation energy is close to the Mg-H bond-breaking energy, one can conclude that breaking this bond would be the rate-limiting step of the process.",
journal = "Materials",
title = "The Catalytic Effect of Vanadium on Sorption Properties of MgH2-Based Nanocomposites Obtained Using Low Milling Time",
pages = "5480",
volume = "16",
number = "15",
doi = "10.3390/ma16155480",
url = "https://hdl.handle.net/21.15107/rcub_dais_15140"
}

Sekulić, Z., Grbović Novaković, J., Babić, B., Prvulović, M., Milanović, I., Novaković, N., Rajnović, D., Filipović, N.,& Asanović, V.. (2023). The Catalytic Effect of Vanadium on Sorption Properties of MgH2-Based Nanocomposites Obtained Using Low Milling Time. in Materials, 16(15), 5480.
https://doi.org/10.3390/ma16155480
https://hdl.handle.net/21.15107/rcub_dais_15140

Sekulić Z, Grbović Novaković J, Babić B, Prvulović M, Milanović I, Novaković N, Rajnović D, Filipović N, Asanović V. The Catalytic Effect of Vanadium on Sorption Properties of MgH2-Based Nanocomposites Obtained Using Low Milling Time. in Materials. 2023;16(15):5480.
doi:10.3390/ma16155480
https://hdl.handle.net/21.15107/rcub_dais_15140 .

Sekulić, Zorana, Grbović Novaković, Jasmina, Babić, Bojana, Prvulović, Milica, Milanović, Igor, Novaković, Nikola, Rajnović, Dragan, Filipović, Nenad, Asanović, Vanja, "The Catalytic Effect of Vanadium on Sorption Properties of MgH2-Based Nanocomposites Obtained Using Low Milling Time" in Materials, 16, no. 15 (2023):5480,
https://doi.org/10.3390/ma16155480 .,
https://hdl.handle.net/21.15107/rcub_dais_15140 .

TY  - JOUR
AU  - Tomić, Nina
AU  - Matić, Tamara
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Boccacccini, Aldo R.
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15195
AB  - Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.
PB  - SAGE Publications Ltd.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties
SP  - 122
EP  - 133
VL  - 38
IS  - 1
DO  - 10.1177/08853282231183109
UR  - https://hdl.handle.net/21.15107/rcub_dais_15195
ER  - 

@article{
author = "Tomić, Nina and Matić, Tamara and Filipović, Nenad and Mitić Ćulafić, Dragana and Boccacccini, Aldo R. and Stevanović, Magdalena",
year = "2023",
abstract = "Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.",
publisher = "SAGE Publications Ltd.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties",
pages = "122-133",
volume = "38",
number = "1",
doi = "10.1177/08853282231183109",
url = "https://hdl.handle.net/21.15107/rcub_dais_15195"
}

Tomić, N., Matić, T., Filipović, N., Mitić Ćulafić, D., Boccacccini, A. R.,& Stevanović, M.. (2023). Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications
SAGE Publications Ltd.., 38(1), 122-133.
https://doi.org/10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_15195

Tomić N, Matić T, Filipović N, Mitić Ćulafić D, Boccacccini AR, Stevanović M. Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications. 2023;38(1):122-133.
doi:10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_15195 .

Tomić, Nina, Matić, Tamara, Filipović, Nenad, Mitić Ćulafić, Dragana, Boccacccini, Aldo R., Stevanović, Magdalena, "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties" in Journal of Biomaterials Applications, 38, no. 1 (2023):122-133,
https://doi.org/10.1177/08853282231183109 .,
https://hdl.handle.net/21.15107/rcub_dais_15195 .

TY  - CONF
AU  - Kuzmanović, Maja
AU  - Filipović, Nenad
AU  - Tomić, Nina
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15225
AB  - As the most abundant protein in the human body, collagen has been drawing the attention of many researchers from the biomedical field for a long time. It can be found as a constituent in versatile commercially available products. In the hydrogel form, it is used often in designing novel platforms for drug delivery or cell growth. On the other side, Selenium nanoparticles (SeNPs) are a relatively new selenium form that has proven records in enhanced antimicrobial, anticancer, antiviral, antioxidative activity, and reduced toxicity compared to other selenium forms. Therefore, in this work, we have examined the effects of different stabilizing agents, used in the preparation of selenium nanoparticles, on the formation, structure, and biocompatibility of composite hydrogel Collagen/SeNPs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), optical microscopy, and cell viability (MTT assay) were used for the characterizations of obtained hydrogels. SeNPs were synthesized by the chemical reduction in the form of colloidal solutions using (i) bovine serum albumin, (ii) chitosan, and(iii) glucose as stabilizing agents.
PB  - Belgrade : Serbian Ceramic Society
C3  - The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles
T1  - The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles
SP  - 86
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_dais_15225
ER  - 

@conference{
author = "Kuzmanović, Maja and Filipović, Nenad and Tomić, Nina and Stevanović, Magdalena",
year = "2023",
abstract = "As the most abundant protein in the human body, collagen has been drawing the attention of many researchers from the biomedical field for a long time. It can be found as a constituent in versatile commercially available products. In the hydrogel form, it is used often in designing novel platforms for drug delivery or cell growth. On the other side, Selenium nanoparticles (SeNPs) are a relatively new selenium form that has proven records in enhanced antimicrobial, anticancer, antiviral, antioxidative activity, and reduced toxicity compared to other selenium forms. Therefore, in this work, we have examined the effects of different stabilizing agents, used in the preparation of selenium nanoparticles, on the formation, structure, and biocompatibility of composite hydrogel Collagen/SeNPs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), optical microscopy, and cell viability (MTT assay) were used for the characterizations of obtained hydrogels. SeNPs were synthesized by the chemical reduction in the form of colloidal solutions using (i) bovine serum albumin, (ii) chitosan, and(iii) glucose as stabilizing agents.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles",
title = "The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles",
pages = "86-86",
url = "https://hdl.handle.net/21.15107/rcub_dais_15225"
}

Kuzmanović, M., Filipović, N., Tomić, N.,& Stevanović, M.. (2023). The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles. in The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles
Belgrade : Serbian Ceramic Society., 86-86.
https://hdl.handle.net/21.15107/rcub_dais_15225

Kuzmanović M, Filipović N, Tomić N, Stevanović M. The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles. in The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles. 2023;:86-86.
https://hdl.handle.net/21.15107/rcub_dais_15225 .

Kuzmanović, Maja, Filipović, Nenad, Tomić, Nina, Stevanović, Magdalena, "The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles" in The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles (2023):86-86,
https://hdl.handle.net/21.15107/rcub_dais_15225 .

TY  - CONF
AU  - Filipović, Nenad
AU  - Tomić, Nina
AU  - Kuzmanović, Maja
AU  - Stojanović, Zoran
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15762
AB  - Nanotechnology, as a most promising approach in material science, has resulted in numerous enhancements and breakthroughs in diverse scientific fields. One of the examples is selenium nanoparticles (SeNPs) which emerged as a new form with some improved properties compared to other Se forms. Among these properties, anticancer, antimicrobial, antioxidative, and reduced toxicity are the most interesting from the aspect of biomedical applications. Due to simplicity, short duration, scalability, and reproducibility, chemical reduction is a synthesis technique very often applied in SeNPs production. The choice of reducing agents, their molar ratio with a precursor, and the choice of stabilizing agents are recognized as determining parameters for the application efficiency of designed SeNPs.
This presentation includes an overview of the results of SeNPs obtained by the reduction of sodium selenite and stabilized with different agents. Furthermore, the effects of synthesis parameters on the properties of obtained particles (size, morphology, crystallinity, stability, surface chemistry) and biological activities such as antimicrobial, antioxidative, and cytotoxicity will be elaborate. In addition, some potential applications of SeNPs will be discussed, with particular reference to the results of in vivo experiments.
PB  - Ljubljana : Inštitut za kovinske materiale in tehnologije
C3  - 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
T1  - Selenium nanoparticles: Effects of particle properties on biological activity
SP  - 32
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_dais_15762
ER  - 

@conference{
author = "Filipović, Nenad and Tomić, Nina and Kuzmanović, Maja and Stojanović, Zoran and Stevanović, Magdalena",
year = "2023",
abstract = "Nanotechnology, as a most promising approach in material science, has resulted in numerous enhancements and breakthroughs in diverse scientific fields. One of the examples is selenium nanoparticles (SeNPs) which emerged as a new form with some improved properties compared to other Se forms. Among these properties, anticancer, antimicrobial, antioxidative, and reduced toxicity are the most interesting from the aspect of biomedical applications. Due to simplicity, short duration, scalability, and reproducibility, chemical reduction is a synthesis technique very often applied in SeNPs production. The choice of reducing agents, their molar ratio with a precursor, and the choice of stabilizing agents are recognized as determining parameters for the application efficiency of designed SeNPs.
This presentation includes an overview of the results of SeNPs obtained by the reduction of sodium selenite and stabilized with different agents. Furthermore, the effects of synthesis parameters on the properties of obtained particles (size, morphology, crystallinity, stability, surface chemistry) and biological activities such as antimicrobial, antioxidative, and cytotoxicity will be elaborate. In addition, some potential applications of SeNPs will be discussed, with particular reference to the results of in vivo experiments.",
publisher = "Ljubljana : Inštitut za kovinske materiale in tehnologije",
journal = "28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology",
title = "Selenium nanoparticles: Effects of particle properties on biological activity",
pages = "32-32",
url = "https://hdl.handle.net/21.15107/rcub_dais_15762"
}

Filipović, N., Tomić, N., Kuzmanović, M., Stojanović, Z.,& Stevanović, M.. (2023). Selenium nanoparticles: Effects of particle properties on biological activity. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
Ljubljana : Inštitut za kovinske materiale in tehnologije., 32-32.
https://hdl.handle.net/21.15107/rcub_dais_15762

Filipović N, Tomić N, Kuzmanović M, Stojanović Z, Stevanović M. Selenium nanoparticles: Effects of particle properties on biological activity. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology. 2023;:32-32.
https://hdl.handle.net/21.15107/rcub_dais_15762 .

Filipović, Nenad, Tomić, Nina, Kuzmanović, Maja, Stojanović, Zoran, Stevanović, Magdalena, "Selenium nanoparticles: Effects of particle properties on biological activity" in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology (2023):32-32,
https://hdl.handle.net/21.15107/rcub_dais_15762 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15749
AB  - There is a growing need for multifunctional components in material design, especially in tissue engineering. Among many natural compounds, polyphenols are gaining interest as biologically active additives of novel materials. Stilbenoid polyphenol resveratrol is one of the most known, mainly because of its antioxidative, antibacterial, anticancer, neuroprotective and other beneficial effects. To allow most efficient use of resveratrol, several nanoformulations have shown promise, but most of them also involve other, toxic or expensive compounds, beside resveratrol. During our research, we synthetized particles of pure resveratrol, in an elongated, nanobelt-like form. The shape of the particles, as well as absence of any polymer or other carriers makes these particles easy to handle for further implementation. For preliminary assessment of the functionality of these particles, several assays were employed. DPPH, TBA and FRAP assay proved antioxidative potential of obtained particles by several mechanisms in the concentration range from 1 μg/mL to 10 μg/mL. Artemia salina in vivo bioassay was used to show high bioactivity and also biocompatibility of the particle suspension. All of the results indicated that there is a high potential for use of these resv
PB  - Ljubljana : Inštitut za kovinske materiale in tehnologije
C3  - 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
T1  - Antioxidative Resveratrol Particles as a Bioactive Component for Material Design
SP  - 87
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_dais_15749
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić Ćulafić, Dragana and Stevanović, Magdalena",
year = "2023",
abstract = "There is a growing need for multifunctional components in material design, especially in tissue engineering. Among many natural compounds, polyphenols are gaining interest as biologically active additives of novel materials. Stilbenoid polyphenol resveratrol is one of the most known, mainly because of its antioxidative, antibacterial, anticancer, neuroprotective and other beneficial effects. To allow most efficient use of resveratrol, several nanoformulations have shown promise, but most of them also involve other, toxic or expensive compounds, beside resveratrol. During our research, we synthetized particles of pure resveratrol, in an elongated, nanobelt-like form. The shape of the particles, as well as absence of any polymer or other carriers makes these particles easy to handle for further implementation. For preliminary assessment of the functionality of these particles, several assays were employed. DPPH, TBA and FRAP assay proved antioxidative potential of obtained particles by several mechanisms in the concentration range from 1 μg/mL to 10 μg/mL. Artemia salina in vivo bioassay was used to show high bioactivity and also biocompatibility of the particle suspension. All of the results indicated that there is a high potential for use of these resv",
publisher = "Ljubljana : Inštitut za kovinske materiale in tehnologije",
journal = "28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology",
title = "Antioxidative Resveratrol Particles as a Bioactive Component for Material Design",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_dais_15749"
}

Tomić, N., Filipović, N., Mitić Ćulafić, D.,& Stevanović, M.. (2023). Antioxidative Resveratrol Particles as a Bioactive Component for Material Design. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
Ljubljana : Inštitut za kovinske materiale in tehnologije., 87-87.
https://hdl.handle.net/21.15107/rcub_dais_15749

Tomić N, Filipović N, Mitić Ćulafić D, Stevanović M. Antioxidative Resveratrol Particles as a Bioactive Component for Material Design. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_dais_15749 .

Tomić, Nina, Filipović, Nenad, Mitić Ćulafić, Dragana, Stevanović, Magdalena, "Antioxidative Resveratrol Particles as a Bioactive Component for Material Design" in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology (2023):87-87,
https://hdl.handle.net/21.15107/rcub_dais_15749 .

TY  - JOUR
AU  - Tomić, Nina
AU  - Matić, Tamara
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Boccacccini, Aldo R.
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14705
AB  - Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.
PB  - SAGE Publications Ltd.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties
SP  - 122
EP  - 133
VL  - 38
IS  - 1
DO  - 10.1177/08853282231183109
UR  - https://hdl.handle.net/21.15107/rcub_dais_14705
ER  - 

@article{
author = "Tomić, Nina and Matić, Tamara and Filipović, Nenad and Mitić Ćulafić, Dragana and Boccacccini, Aldo R. and Stevanović, Magdalena",
year = "2023",
abstract = "Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.",
publisher = "SAGE Publications Ltd.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties",
pages = "122-133",
volume = "38",
number = "1",
doi = "10.1177/08853282231183109",
url = "https://hdl.handle.net/21.15107/rcub_dais_14705"
}

Tomić, N., Matić, T., Filipović, N., Mitić Ćulafić, D., Boccacccini, A. R.,& Stevanović, M.. (2023). Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications
SAGE Publications Ltd.., 38(1), 122-133.
https://doi.org/10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_14705

Tomić N, Matić T, Filipović N, Mitić Ćulafić D, Boccacccini AR, Stevanović M. Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications. 2023;38(1):122-133.
doi:10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_14705 .

Tomić, Nina, Matić, Tamara, Filipović, Nenad, Mitić Ćulafić, Dragana, Boccacccini, Aldo R., Stevanović, Magdalena, "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties" in Journal of Biomaterials Applications, 38, no. 1 (2023):122-133,
https://doi.org/10.1177/08853282231183109 .,
https://hdl.handle.net/21.15107/rcub_dais_14705 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Ganić, Tea
AU  - Klyagin, Sergey
AU  - Osmolovskiy, Alexander
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15635
AB  - Biomaterial implant contaminations are the most common nosocomial infections. Especially complicated to treat are the ones caused by microbial biofilms. Biofilms are structures comprised of surface-attached cells protected by self-produced polymeric matrix, and are present in more than 80% of persistent infections. Pseudomonas aeruginosa biofilms are exceptionally difficult to resolve, due to high capability of this bacteria for producing biofilm and acquiring antibiotic resistance. In order to prevent biofilm formation and reduce the unnecessary use of antibiotics, various nanomaterials and natural antibiofilm agents are being given more attention in tissue engineering in the last decade. Bioactive polyphenolic compounds have potential for use in biomaterials as multifunctional additives. Among polyphenols, resveratrol is one of the most known, but its’ use is limited by low bioavailability and difficulty of delivery. In our previous research, we created resveratrol nanobelt-like particles of shape convenient for handling and scaffold coating. Here we investigated their potential for inhibiting growth and biofilm formation of several strains of Pseudomonas aeruginosa. Microdilution assay and resazurin staining, as well as agar plating were used to estimate minimal inhibitory and minimal bactericidal concentration. MTT method was performed to more accurately measure the influence of various concentrations on bacterial growth. Crystal violet assay and RT PCR analysis determined the amount of biofilm formation and effect on gene expression. The effect varied among the strains, but most of the tested concentrations, including the lowest ones, led to at least 40% of inhibition of biofilm formation. Concentration of 800 μg/ml achieved almost 90% inhibition of biofilm formation in ATCC reference strain, indicating potential of this material for further use for such purpose in biomaterial design.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms
SP  - 8
EP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_dais_15635
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić Ćulafić, Dragana and Ganić, Tea and Klyagin, Sergey and Osmolovskiy, Alexander and Stevanović, Magdalena",
year = "2023",
abstract = "Biomaterial implant contaminations are the most common nosocomial infections. Especially complicated to treat are the ones caused by microbial biofilms. Biofilms are structures comprised of surface-attached cells protected by self-produced polymeric matrix, and are present in more than 80% of persistent infections. Pseudomonas aeruginosa biofilms are exceptionally difficult to resolve, due to high capability of this bacteria for producing biofilm and acquiring antibiotic resistance. In order to prevent biofilm formation and reduce the unnecessary use of antibiotics, various nanomaterials and natural antibiofilm agents are being given more attention in tissue engineering in the last decade. Bioactive polyphenolic compounds have potential for use in biomaterials as multifunctional additives. Among polyphenols, resveratrol is one of the most known, but its’ use is limited by low bioavailability and difficulty of delivery. In our previous research, we created resveratrol nanobelt-like particles of shape convenient for handling and scaffold coating. Here we investigated their potential for inhibiting growth and biofilm formation of several strains of Pseudomonas aeruginosa. Microdilution assay and resazurin staining, as well as agar plating were used to estimate minimal inhibitory and minimal bactericidal concentration. MTT method was performed to more accurately measure the influence of various concentrations on bacterial growth. Crystal violet assay and RT PCR analysis determined the amount of biofilm formation and effect on gene expression. The effect varied among the strains, but most of the tested concentrations, including the lowest ones, led to at least 40% of inhibition of biofilm formation. Concentration of 800 μg/ml achieved almost 90% inhibition of biofilm formation in ATCC reference strain, indicating potential of this material for further use for such purpose in biomaterial design.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms",
pages = "8-8",
url = "https://hdl.handle.net/21.15107/rcub_dais_15635"
}

Tomić, N., Filipović, N., Mitić Ćulafić, D., Ganić, T., Klyagin, S., Osmolovskiy, A.,& Stevanović, M.. (2023). Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 8-8.
https://hdl.handle.net/21.15107/rcub_dais_15635

Tomić N, Filipović N, Mitić Ćulafić D, Ganić T, Klyagin S, Osmolovskiy A, Stevanović M. Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:8-8.
https://hdl.handle.net/21.15107/rcub_dais_15635 .

Tomić, Nina, Filipović, Nenad, Mitić Ćulafić, Dragana, Ganić, Tea, Klyagin, Sergey, Osmolovskiy, Alexander, Stevanović, Magdalena, "Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):8-8,
https://hdl.handle.net/21.15107/rcub_dais_15635 .

TY  - CONF
AU  - Prvulović, Milica
AU  - Babić, Bojana
AU  - Filipović, Nenad
AU  - Mravik, Željko
AU  - Milošević Govedarović, Sanja
AU  - Sekulić, Zorana
AU  - Milanović, Igor
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15640
AB  - The effect of pure Ni addition (5 wt.%) in MgH2 powder was investigated mechanochemically for short milling times (15, 30, and 45 min). Obtained MgH2-Ni system was characterized by XRD, SEM-EDS, PSD, DSC, and TPD. Compared to pure MgH2, uniform distribution of nickel reduces the temperature of H2 desorption by more than 100 °C. It is shown that influence of two important processes, grinding and catalysis, may be followed separately. We can conclude that the catalysis of H2 desorption by Ni particles on MgH2 matrix is the dominant effect for the investigated short milling times.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Hydrogen storage properties of MgH2-Ni system
SP  - 39
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_dais_15640
ER  - 

@conference{
author = "Prvulović, Milica and Babić, Bojana and Filipović, Nenad and Mravik, Željko and Milošević Govedarović, Sanja and Sekulić, Zorana and Milanović, Igor",
year = "2023",
abstract = "The effect of pure Ni addition (5 wt.%) in MgH2 powder was investigated mechanochemically for short milling times (15, 30, and 45 min). Obtained MgH2-Ni system was characterized by XRD, SEM-EDS, PSD, DSC, and TPD. Compared to pure MgH2, uniform distribution of nickel reduces the temperature of H2 desorption by more than 100 °C. It is shown that influence of two important processes, grinding and catalysis, may be followed separately. We can conclude that the catalysis of H2 desorption by Ni particles on MgH2 matrix is the dominant effect for the investigated short milling times.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Hydrogen storage properties of MgH2-Ni system",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_dais_15640"
}

Prvulović, M., Babić, B., Filipović, N., Mravik, Ž., Milošević Govedarović, S., Sekulić, Z.,& Milanović, I.. (2023). Hydrogen storage properties of MgH2-Ni system. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 39-39.
https://hdl.handle.net/21.15107/rcub_dais_15640

Prvulović M, Babić B, Filipović N, Mravik Ž, Milošević Govedarović S, Sekulić Z, Milanović I. Hydrogen storage properties of MgH2-Ni system. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:39-39.
https://hdl.handle.net/21.15107/rcub_dais_15640 .

Prvulović, Milica, Babić, Bojana, Filipović, Nenad, Mravik, Željko, Milošević Govedarović, Sanja, Sekulić, Zorana, Milanović, Igor, "Hydrogen storage properties of MgH2-Ni system" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):39-39,
https://hdl.handle.net/21.15107/rcub_dais_15640 .

TY  - CONF
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena M.
AU  - Arsenović-Ranin, Nevena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5133
UR  - https://dais.sanu.ac.rs/123456789/15263
AB  - Colistin is used as a “last resort” drug in the treatmentof carbapenem-resistant Acinetobacter baumanniiinfections. Though not the most convenient option due tothe serious side effects, this antibiotic can be very efficientif administered properly (Garnacho-Montero and Timsit,2019). However, for several years now, we are witnessinga steady emergence of colistin-resistant (ColR) strains, withthe constant threat of a sudden burst worldwide (Nowak etal., 2017). Having in mind that these strains are usuallynon-susceptible to any of the existing treatments, i.e.pandrug-resistant (PDR), the development of newtherapeutic strategies is desperately needed.In the present study, we showed that colistinsusceptibility of ColR A. baumannii strains can be fullyrestored in the presence of very low amounts of seleniumnanoparticles (SeNPs). To demonstrate potent synergisticinteractions between colistin and SeNPs, we performedcheckerboard and time-kill analyses.
PB  - Macedonian Pharmaceutical Association
C3  - Macedonian Pharmaceutical Bulletin
T1  - Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin
SP  - 295
EP  - 296
VL  - 68 (Suppl. 1)
DO  - 10.33320/maced.pharm.bull.2022.68.03.142
UR  - https://hdl.handle.net/21.15107/rcub_dais_15263
ER  - 

@conference{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena M. and Arsenović-Ranin, Nevena and Milenković, Marina",
year = "2022",
abstract = "Colistin is used as a “last resort” drug in the treatmentof carbapenem-resistant Acinetobacter baumanniiinfections. Though not the most convenient option due tothe serious side effects, this antibiotic can be very efficientif administered properly (Garnacho-Montero and Timsit,2019). However, for several years now, we are witnessinga steady emergence of colistin-resistant (ColR) strains, withthe constant threat of a sudden burst worldwide (Nowak etal., 2017). Having in mind that these strains are usuallynon-susceptible to any of the existing treatments, i.e.pandrug-resistant (PDR), the development of newtherapeutic strategies is desperately needed.In the present study, we showed that colistinsusceptibility of ColR A. baumannii strains can be fullyrestored in the presence of very low amounts of seleniumnanoparticles (SeNPs). To demonstrate potent synergisticinteractions between colistin and SeNPs, we performedcheckerboard and time-kill analyses.",
publisher = "Macedonian Pharmaceutical Association",
journal = "Macedonian Pharmaceutical Bulletin",
title = "Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin",
pages = "295-296",
volume = "68 (Suppl. 1)",
doi = "10.33320/maced.pharm.bull.2022.68.03.142",
url = "https://hdl.handle.net/21.15107/rcub_dais_15263"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M. M., Arsenović-Ranin, N.,& Milenković, M.. (2022). Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin. in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68 (Suppl. 1), 295-296.
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.142
https://hdl.handle.net/21.15107/rcub_dais_15263

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović MM, Arsenović-Ranin N, Milenković M. Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin. in Macedonian Pharmaceutical Bulletin. 2022;68 (Suppl. 1):295-296.
doi:10.33320/maced.pharm.bull.2022.68.03.142
https://hdl.handle.net/21.15107/rcub_dais_15263 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena M., Arsenović-Ranin, Nevena, Milenković, Marina, "Selenium nanoparticles render pandrug-resistant Acinetobacter  baumannii susceptible to colistin" in Macedonian Pharmaceutical Bulletin, 68 (Suppl. 1) (2022):295-296,
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.142 .,
https://hdl.handle.net/21.15107/rcub_dais_15263 .

TY  - CHAP
AU  - Filipović, Nenad
AU  - Tomić, Nina
AU  - Kuzmanović, Maja
AU  - Stevanović, Magdalena
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13184
AB  - One of the major issues related to medical devices and especially urinary stents are infections caused by different strains of bacteria and fungi, mainly in light of the recent rise in microbial resistance to existing antibiotics. Lately, it has been shown that nanomaterials could be superior alternatives to conventional antibiotics. Generally, nanoparticles are used for many applications in the biomedical field primarily due to the ability to adjust and control their physicochemical properties as well as their great reactivity due to the large surface-to-volume ratio. This has led to the formation of a new research field called nanomedicine which can be defined as the use of nanotechnology and nanomaterials in diagnostics, imaging, observing, prevention, control, and treatment of diseases. For example, coverings or coatings based on nanomaterials are now seen as a promising strategy for preventing or treating biofilms formation on healthcare kits, implants, and medical devices. Toxicity, inappropriate delivery, or degradation of conventionally used drugs for the treatment of infections may be avoided by using nanoparticles without or with encapsulated/immobilized active substances. Most of the materials which are used and examined for the preparation of the nanoparticles with encapsulated/immobilized active substances or smart reactive nanomaterials with antimicrobial effects are polymers, naturally derived antimicrobials, metal-based and non-metallic materials. This chapter provides an overview of the current state and future perspectives of the nanoparticle-based systems based on these materials for prevention, control, or elimination of biofilm-related infections on urinary stents. It also addresses manufacturing conditions indicating the huge potential for the improvement of existing and development of new promising stent solutions.
PB  - Cham : Springer International Publishing
T2  - Urinary Stents
T1  - Nanoparticles: Potential for Use to Prevent Infections
SP  - 325
EP  - 339
DO  - 10.1007/978-3-031-04484-7_26
UR  - https://hdl.handle.net/21.15107/rcub_dais_13184
ER  - 

@inbook{
editor = "Soria, Federico, Rako, Duje, de Graaf, Petra",
author = "Filipović, Nenad and Tomić, Nina and Kuzmanović, Maja and Stevanović, Magdalena",
year = "2022",
abstract = "One of the major issues related to medical devices and especially urinary stents are infections caused by different strains of bacteria and fungi, mainly in light of the recent rise in microbial resistance to existing antibiotics. Lately, it has been shown that nanomaterials could be superior alternatives to conventional antibiotics. Generally, nanoparticles are used for many applications in the biomedical field primarily due to the ability to adjust and control their physicochemical properties as well as their great reactivity due to the large surface-to-volume ratio. This has led to the formation of a new research field called nanomedicine which can be defined as the use of nanotechnology and nanomaterials in diagnostics, imaging, observing, prevention, control, and treatment of diseases. For example, coverings or coatings based on nanomaterials are now seen as a promising strategy for preventing or treating biofilms formation on healthcare kits, implants, and medical devices. Toxicity, inappropriate delivery, or degradation of conventionally used drugs for the treatment of infections may be avoided by using nanoparticles without or with encapsulated/immobilized active substances. Most of the materials which are used and examined for the preparation of the nanoparticles with encapsulated/immobilized active substances or smart reactive nanomaterials with antimicrobial effects are polymers, naturally derived antimicrobials, metal-based and non-metallic materials. This chapter provides an overview of the current state and future perspectives of the nanoparticle-based systems based on these materials for prevention, control, or elimination of biofilm-related infections on urinary stents. It also addresses manufacturing conditions indicating the huge potential for the improvement of existing and development of new promising stent solutions.",
publisher = "Cham : Springer International Publishing",
journal = "Urinary Stents",
booktitle = "Nanoparticles: Potential for Use to Prevent Infections",
pages = "325-339",
doi = "10.1007/978-3-031-04484-7_26",
url = "https://hdl.handle.net/21.15107/rcub_dais_13184"
}

Soria, F., Rako, D., de Graaf, P., Filipović, N., Tomić, N., Kuzmanović, M.,& Stevanović, M.. (2022). Nanoparticles: Potential for Use to Prevent Infections. in Urinary Stents
Cham : Springer International Publishing., 325-339.
https://doi.org/10.1007/978-3-031-04484-7_26
https://hdl.handle.net/21.15107/rcub_dais_13184

Soria F, Rako D, de Graaf P, Filipović N, Tomić N, Kuzmanović M, Stevanović M. Nanoparticles: Potential for Use to Prevent Infections. in Urinary Stents. 2022;:325-339.
doi:10.1007/978-3-031-04484-7_26
https://hdl.handle.net/21.15107/rcub_dais_13184 .

Soria, Federico, Rako, Duje, de Graaf, Petra, Filipović, Nenad, Tomić, Nina, Kuzmanović, Maja, Stevanović, Magdalena, "Nanoparticles: Potential for Use to Prevent Infections" in Urinary Stents (2022):325-339,
https://doi.org/10.1007/978-3-031-04484-7_26 .,
https://hdl.handle.net/21.15107/rcub_dais_13184 .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Tomić, Nina
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Stampfl, Juergen
AU  - Boccaccini, Aldo
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13579
AB  - Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
SP  - 1800
EP  - 1811
VL  - 36
IS  - 10
DO  - 10.1177/08853282211073731
UR  - https://hdl.handle.net/21.15107/rcub_dais_13579
ER  - 

@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccini, Aldo",
year = "2022",
abstract = "Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles",
pages = "1800-1811",
volume = "36",
number = "10",
doi = "10.1177/08853282211073731",
url = "https://hdl.handle.net/21.15107/rcub_dais_13579"
}

Stevanović, M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccini, A.. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications, 36(10), 1800-1811.
https://doi.org/10.1177/08853282211073731
https://hdl.handle.net/21.15107/rcub_dais_13579

Stevanović M, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccini A. Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications. 2022;36(10):1800-1811.
doi:10.1177/08853282211073731
https://hdl.handle.net/21.15107/rcub_dais_13579 .

Stevanović, Magdalena, Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccini, Aldo, "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles" in Journal of Biomaterials Applications, 36, no. 10 (2022):1800-1811,
https://doi.org/10.1177/08853282211073731 .,
https://hdl.handle.net/21.15107/rcub_dais_13579 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Stevanović, Magdalena
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13501
AB  - Natural chemicals and earth elements are increasingly used in research as basis for novel materials intended for use in medicine. Among phytopharmaceuticals, more specifically polyphenols, resveratrol is known for its’ antioxidative, anticancer, antimicrobial, and other beneficial effects. Selenium, an essential trace element, is lately being recognized in nanoparticle form as less toxic and equally or more efficient than commercially available forms. The synergy of these two agents have not been shown until lately, when their synergistic antioxidative and gene expression effects were investigated for the purpose of treating Alcheimer disease. During our previous research, we have successfully synthesized pure resveratrol particles, as well as selenium nanoparticles (SeNPs). Both of them were separately investigated regarding their biological activities. The first step in evaluation of their possible synergistic antioxidative effect was obtaining the stable composite of these two materials. Synthesis parameters and processing methods were varied, and obtained suspensions assessed by their macroscopic and microscopic characteristics. The sample with both components homogeneously distributed in the particle form, was chosen for further experiments. Ultraviolet-visible (UV-Vis) spectrophotometry and Fourier transform infrared spectroscopy (FTIR) were used to characterize sample, and antioxidative activity (by DPPH reduction assay), and cytocompatibility (using MTT cytotoxicity assay) were additionally determined. Results showed improved cytocompatibility as compared to pure resveratrol particles, and preserved, significantly high antioxidative potential.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia
T1  - Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility
SP  - 5
EP  - 5
UR  - https://hdl.handle.net/21.15107/rcub_dais_13501
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić Ćulafić, Dragana and Stevanović, Magdalena",
year = "2022",
abstract = "Natural chemicals and earth elements are increasingly used in research as basis for novel materials intended for use in medicine. Among phytopharmaceuticals, more specifically polyphenols, resveratrol is known for its’ antioxidative, anticancer, antimicrobial, and other beneficial effects. Selenium, an essential trace element, is lately being recognized in nanoparticle form as less toxic and equally or more efficient than commercially available forms. The synergy of these two agents have not been shown until lately, when their synergistic antioxidative and gene expression effects were investigated for the purpose of treating Alcheimer disease. During our previous research, we have successfully synthesized pure resveratrol particles, as well as selenium nanoparticles (SeNPs). Both of them were separately investigated regarding their biological activities. The first step in evaluation of their possible synergistic antioxidative effect was obtaining the stable composite of these two materials. Synthesis parameters and processing methods were varied, and obtained suspensions assessed by their macroscopic and microscopic characteristics. The sample with both components homogeneously distributed in the particle form, was chosen for further experiments. Ultraviolet-visible (UV-Vis) spectrophotometry and Fourier transform infrared spectroscopy (FTIR) were used to characterize sample, and antioxidative activity (by DPPH reduction assay), and cytocompatibility (using MTT cytotoxicity assay) were additionally determined. Results showed improved cytocompatibility as compared to pure resveratrol particles, and preserved, significantly high antioxidative potential.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia",
title = "Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility",
pages = "5-5",
url = "https://hdl.handle.net/21.15107/rcub_dais_13501"
}

Tomić, N., Filipović, N., Mitić Ćulafić, D.,& Stevanović, M.. (2022). Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 5-5.
https://hdl.handle.net/21.15107/rcub_dais_13501

Tomić N, Filipović N, Mitić Ćulafić D, Stevanović M. Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia. 2022;:5-5.
https://hdl.handle.net/21.15107/rcub_dais_13501 .

Tomić, Nina, Filipović, Nenad, Mitić Ćulafić, Dragana, Stevanović, Magdalena, "Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility" in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia (2022):5-5,
https://hdl.handle.net/21.15107/rcub_dais_13501 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić-Ćulafić, Dragana
AU  - Đokić, Ana
AU  - Stevanović, Magdalena
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13605
AB  - Selenium is an especially important element for the functioning of antioxidative enzymes, making it one of the essential micronutrients. Although selenium is required in extremely small amounts, often it is lacking from the organism. However, inorganic and organic sources of selenium are known to possess considerable toxicity. Recently, the nanoparticle form of selenium has been shown to exhibit lesser toxicity, and also better antimicrobial and other biological effects, than selenium-containing compounds. Nanotechnology has already found application in many fields, for improving material properties for various purposes, among them, in the process of improving food characteristics. It is possible that selenium nanoparticles can be applied as antimicrobial agents in food, and there has been growing interest in this area of research. We have synthesized selenium nanoparticles (SeNPs) by chemical reduction method, using sodium selenite, ascorbic acid, and bovine serum albumin. The antimicrobial activity of these particles has been assessed by minimum inhibitory concentration assay and colony-forming units count, on Listeria monocytogenes and Salmonella enterica, which are common bacterial food contaminants. As intestinal microbiota, especially probiotic bacteria, have been shown to play important role in organism functioning, we also tested how SeNPs influence Lactobacillus rhamosus and Lactobacillus plantarum. To evaluate cytotoxicity, we used MTT assay on the human intestinal cell line (HT-29). Antioxidative properties were assessed by DPPH assay. SeNPs showed higher antibacterial activity on pathogenic bacteria than on probiotic strains. Cytotoxic effect was present only in the highest tested concentrations. The antioxidative activity was considerable, resulting in above 80% reduction of DPPH free radical, at 1 g/ml of SeNPs. Based on these results, SeNPs are a promising subject for further research in developing materials for food packaging or as antimicrobial selenium additives.
PB  - Novi Sad : University of Novi Sad, Faculty of Technology
C3  - Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
T1  - Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells
SP  - 129
EP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_dais_13605
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić-Ćulafić, Dragana and Đokić, Ana and Stevanović, Magdalena",
year = "2022",
abstract = "Selenium is an especially important element for the functioning of antioxidative enzymes, making it one of the essential micronutrients. Although selenium is required in extremely small amounts, often it is lacking from the organism. However, inorganic and organic sources of selenium are known to possess considerable toxicity. Recently, the nanoparticle form of selenium has been shown to exhibit lesser toxicity, and also better antimicrobial and other biological effects, than selenium-containing compounds. Nanotechnology has already found application in many fields, for improving material properties for various purposes, among them, in the process of improving food characteristics. It is possible that selenium nanoparticles can be applied as antimicrobial agents in food, and there has been growing interest in this area of research. We have synthesized selenium nanoparticles (SeNPs) by chemical reduction method, using sodium selenite, ascorbic acid, and bovine serum albumin. The antimicrobial activity of these particles has been assessed by minimum inhibitory concentration assay and colony-forming units count, on Listeria monocytogenes and Salmonella enterica, which are common bacterial food contaminants. As intestinal microbiota, especially probiotic bacteria, have been shown to play important role in organism functioning, we also tested how SeNPs influence Lactobacillus rhamosus and Lactobacillus plantarum. To evaluate cytotoxicity, we used MTT assay on the human intestinal cell line (HT-29). Antioxidative properties were assessed by DPPH assay. SeNPs showed higher antibacterial activity on pathogenic bacteria than on probiotic strains. Cytotoxic effect was present only in the highest tested concentrations. The antioxidative activity was considerable, resulting in above 80% reduction of DPPH free radical, at 1 g/ml of SeNPs. Based on these results, SeNPs are a promising subject for further research in developing materials for food packaging or as antimicrobial selenium additives.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Technology",
journal = "Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad",
title = "Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells",
pages = "129-129",
url = "https://hdl.handle.net/21.15107/rcub_dais_13605"
}

Tomić, N., Filipović, N., Mitić-Ćulafić, D., Đokić, A.,& Stevanović, M.. (2022). Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
Novi Sad : University of Novi Sad, Faculty of Technology., 129-129.
https://hdl.handle.net/21.15107/rcub_dais_13605

Tomić N, Filipović N, Mitić-Ćulafić D, Đokić A, Stevanović M. Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad. 2022;:129-129.
https://hdl.handle.net/21.15107/rcub_dais_13605 .

Tomić, Nina, Filipović, Nenad, Mitić-Ćulafić, Dragana, Đokić, Ana, Stevanović, Magdalena, "Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells" in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad (2022):129-129,
https://hdl.handle.net/21.15107/rcub_dais_13605 .

TY  - CONF
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
AU  - Veljković, F.
AU  - Martinović, J.
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Guševac Stojanović, Ivana
PY  - 2022
UR  - https://www.ecnp.eu/congress2022/ECNPcongress/programme/programme#!abstractdetails/0000505040
UR  - https://dais.sanu.ac.rs/123456789/13625
AB  - Introduction: As a result of human activity, the amount of plastic waste has increased immensely in the environment. While the main concern has been orientated toward visible litter, more recently fragments of plastic less than 5 mm in diameter, called microplastic particles (MP), raised the attention due to their ability to induce toxic effects in organisms. The toxicity of MP increases with a decrease in size since smaller sized particles are absorbed systematically and accumulated in different organs/tissues, including brain.

Aim: Present study is designed to compare possible toxic acute effects of different sized MP, originating from polyethylene-terephthalate bottles, on toxicity signs, sensory-motor functions and relative brain weight in young male Wistar rats.

Methods: Rats (n = 24) were divided in four experimental groups: (I) intact (controls); Q that orally received a 2.5 ml of Milli-Q; P1/P2 that were respectively orally administrated with 35 mg/kg of MP in median diameter of either 40 or 70 μm dissolved in a 2.5 ml of Milli-Q. 24 h after treatments, using toxicity clinical test, we assessed the agitation, convulsion, piloerection, sleepiness and lethargy. Parameters were valued as: – (no effect), + (mild effect), ++ (moderate effect) and +++ (major effect). Then, we evaluated sensory-motor functions by monitoring: consciousness (scored 0/1 point), respiration (scored 0/1), spontaneous activity (scored 0/1/2/3), forepaw outstretching (scored 0/1/2), climbing (scored 0/1), visual placing (scored 0/1), cage grasp (scored 0/1), gait posture (scored 0/1/2), geotaxis (scored 0/1), hearing (scored 0/1) and pacing/circling (scored 0/1). Total score was expressed as the sum of average scores of all investigated parameters and graded on the scale from 0 to 15. Then, rats were weighed and sacrificed, while their brains were carefully removed and weighed. The relative brain weights were calculated as the ratio of brain (wet tissue, mg) weight to body weight (g). For toxicity clinical testing a descriptive analysis was used while other data were analysed by One-way analysis of variance followed by Tukey’s posthoc test. Results were considered significant at p < 0.05.
Results: Clinical toxicity signs were similar between groups while results of sensory-motor test point to unaltered sensory-motor functions upon MP treatments compared to controls (F (3, 20) = 0.9538; P = 0.4336). The absence of changes might be attributed to acute treatment, brief evaluation period and lack of cumulative effect upon MP treatments. Nonetheless, only smaller sized MP led to an increase in relative brain weight compared to controls (F (3, 20) = 3.425; P = 0.0369; p < 0.05) which might indicate the capability of smaller sized MP to cross blood-brain barrier and be accumulated in the brain inducing oxidative stress and inflammatory response. Although larger sized MP did not cause changes in relative brain weight, the possible modulations of additional parameters that were not the focus of this study should not be omitted.
Conclusion: According to the presented results, even a smaller sized MP applied in a single dose could be harmful to health, thus, greater control of plastic usage and disposal is essential for humanity.
PB  - ECNP
C3  - 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
T1  - Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats
SP  - P.0244
UR  - https://hdl.handle.net/21.15107/rcub_dais_13625
ER  - 

@conference{
author = "Stojanović, Zoran and Drakulić, Dunja and Veljković, F. and Martinović, J. and Todorović, A. and Filipović, Nenad and Stevanović, Magdalena and Guševac Stojanović, Ivana",
year = "2022",
abstract = "Introduction: As a result of human activity, the amount of plastic waste has increased immensely in the environment. While the main concern has been orientated toward visible litter, more recently fragments of plastic less than 5 mm in diameter, called microplastic particles (MP), raised the attention due to their ability to induce toxic effects in organisms. The toxicity of MP increases with a decrease in size since smaller sized particles are absorbed systematically and accumulated in different organs/tissues, including brain.

Aim: Present study is designed to compare possible toxic acute effects of different sized MP, originating from polyethylene-terephthalate bottles, on toxicity signs, sensory-motor functions and relative brain weight in young male Wistar rats.

Methods: Rats (n = 24) were divided in four experimental groups: (I) intact (controls); Q that orally received a 2.5 ml of Milli-Q; P1/P2 that were respectively orally administrated with 35 mg/kg of MP in median diameter of either 40 or 70 μm dissolved in a 2.5 ml of Milli-Q. 24 h after treatments, using toxicity clinical test, we assessed the agitation, convulsion, piloerection, sleepiness and lethargy. Parameters were valued as: – (no effect), + (mild effect), ++ (moderate effect) and +++ (major effect). Then, we evaluated sensory-motor functions by monitoring: consciousness (scored 0/1 point), respiration (scored 0/1), spontaneous activity (scored 0/1/2/3), forepaw outstretching (scored 0/1/2), climbing (scored 0/1), visual placing (scored 0/1), cage grasp (scored 0/1), gait posture (scored 0/1/2), geotaxis (scored 0/1), hearing (scored 0/1) and pacing/circling (scored 0/1). Total score was expressed as the sum of average scores of all investigated parameters and graded on the scale from 0 to 15. Then, rats were weighed and sacrificed, while their brains were carefully removed and weighed. The relative brain weights were calculated as the ratio of brain (wet tissue, mg) weight to body weight (g). For toxicity clinical testing a descriptive analysis was used while other data were analysed by One-way analysis of variance followed by Tukey’s posthoc test. Results were considered significant at p < 0.05.
Results: Clinical toxicity signs were similar between groups while results of sensory-motor test point to unaltered sensory-motor functions upon MP treatments compared to controls (F (3, 20) = 0.9538; P = 0.4336). The absence of changes might be attributed to acute treatment, brief evaluation period and lack of cumulative effect upon MP treatments. Nonetheless, only smaller sized MP led to an increase in relative brain weight compared to controls (F (3, 20) = 3.425; P = 0.0369; p < 0.05) which might indicate the capability of smaller sized MP to cross blood-brain barrier and be accumulated in the brain inducing oxidative stress and inflammatory response. Although larger sized MP did not cause changes in relative brain weight, the possible modulations of additional parameters that were not the focus of this study should not be omitted.
Conclusion: According to the presented results, even a smaller sized MP applied in a single dose could be harmful to health, thus, greater control of plastic usage and disposal is essential for humanity.",
publisher = "ECNP",
journal = "35the ECNP Congress, 15-18 October 2022, Vienna, Austria",
title = "Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats",
pages = "P.0244",
url = "https://hdl.handle.net/21.15107/rcub_dais_13625"
}

Stojanović, Z., Drakulić, D., Veljković, F., Martinović, J., Todorović, A., Filipović, N., Stevanović, M.,& Guševac Stojanović, I.. (2022). Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
ECNP., P.0244.
https://hdl.handle.net/21.15107/rcub_dais_13625

Stojanović Z, Drakulić D, Veljković F, Martinović J, Todorović A, Filipović N, Stevanović M, Guševac Stojanović I. Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria. 2022;:P.0244.
https://hdl.handle.net/21.15107/rcub_dais_13625 .

Stojanović, Zoran, Drakulić, Dunja, Veljković, F., Martinović, J., Todorović, A., Filipović, Nenad, Stevanović, Magdalena, Guševac Stojanović, Ivana, "Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats" in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria (2022):P.0244,
https://hdl.handle.net/21.15107/rcub_dais_13625 .

TY  - CONF
AU  - Томић, Нина
AU  - Филиповић, Ненад
AU  - Гајић, Ина
AU  - Стевановић, Магдалена
AU  - Митић-Ћулафић, Драгана
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13606
AB  - Биофилм као животна форма представља један од најважнијих фактора вируленције који је заступљен у 80-90% инфекција, а посебно на материјалима који се користе у ортопедији. Бактерије у биофилму су вишеструко отпорније на антибиотике и спољашње факторе, те је превенција развоја биофилма некада једини ефикасан метод спречавања појаве и ширења инфекције. У последње време интензивно се проучавају наноструктуирани материјали отпорни на колонизацију бактерија и развој биофилма. Селен је један од елемената који у својој наноформи показује добре биолошке ефекте, а ресвератрол је популарни полифенол чији се потенцијал за примену у медицини интензивно истражује. Добијањем обе ове компоненте у нано- облицима, отварају се даље могућности комбиновања и стварања композитног материјала са побољшаним особинама, за примену у превенцији биофилма. Ресвератрол и наночестице селена појединачно показују добар, али недовољно истражен антимикробни потенцијал. Након њиховог спајања испитали смо ефекат добијеног композита у спречавању формирања биофилма код више бактеријских врста. Концентрације композита од 6% (и ниже) су доводиле до инхибиције формирања биофилма од најмање 20% код већине тестираних бактерија. Посебно се истиче добар антибиофилм ефекат на сојевима P. aeruginosa, где је на тестираним концентрацијама уочена инхибиција формирања биофилма у опсегу од 20-60%. Такође, антибиофилм ефекат композита је у већини случајева бољи у односу на саме честице у истим концентрацијама.
PB  - Београд : Српско биолошко друштво
C3  - Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
T1  - Антибиофилм активност нанокомпозита на бази ресвератрола и селена
SP  - 252
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_dais_13606
ER  - 

@conference{
author = "Томић, Нина and Филиповић, Ненад and Гајић, Ина and Стевановић, Магдалена and Митић-Ћулафић, Драгана",
year = "2022",
abstract = "Биофилм као животна форма представља један од најважнијих фактора вируленције који је заступљен у 80-90% инфекција, а посебно на материјалима који се користе у ортопедији. Бактерије у биофилму су вишеструко отпорније на антибиотике и спољашње факторе, те је превенција развоја биофилма некада једини ефикасан метод спречавања појаве и ширења инфекције. У последње време интензивно се проучавају наноструктуирани материјали отпорни на колонизацију бактерија и развој биофилма. Селен је један од елемената који у својој наноформи показује добре биолошке ефекте, а ресвератрол је популарни полифенол чији се потенцијал за примену у медицини интензивно истражује. Добијањем обе ове компоненте у нано- облицима, отварају се даље могућности комбиновања и стварања композитног материјала са побољшаним особинама, за примену у превенцији биофилма. Ресвератрол и наночестице селена појединачно показују добар, али недовољно истражен антимикробни потенцијал. Након њиховог спајања испитали смо ефекат добијеног композита у спречавању формирања биофилма код више бактеријских врста. Концентрације композита од 6% (и ниже) су доводиле до инхибиције формирања биофилма од најмање 20% код већине тестираних бактерија. Посебно се истиче добар антибиофилм ефекат на сојевима P. aeruginosa, где је на тестираним концентрацијама уочена инхибиција формирања биофилма у опсегу од 20-60%. Такође, антибиофилм ефекат композита је у већини случајева бољи у односу на саме честице у истим концентрацијама.",
publisher = "Београд : Српско биолошко друштво",
journal = "Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.",
title = "Антибиофилм активност нанокомпозита на бази ресвератрола и селена",
pages = "252-252",
url = "https://hdl.handle.net/21.15107/rcub_dais_13606"
}

Томић, Н., Филиповић, Н., Гајић, И., Стевановић, М.,& Митић-Ћулафић, Д.. (2022). Антибиофилм активност нанокомпозита на бази ресвератрола и селена. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
Београд : Српско биолошко друштво., 252-252.
https://hdl.handle.net/21.15107/rcub_dais_13606

Томић Н, Филиповић Н, Гајић И, Стевановић М, Митић-Ћулафић Д. Антибиофилм активност нанокомпозита на бази ресвератрола и селена. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.. 2022;:252-252.
https://hdl.handle.net/21.15107/rcub_dais_13606 .

Томић, Нина, Филиповић, Ненад, Гајић, Ина, Стевановић, Магдалена, Митић-Ћулафић, Драгана, "Антибиофилм активност нанокомпозита на бази ресвератрола и селена" in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022. (2022):252-252,
https://hdl.handle.net/21.15107/rcub_dais_13606 .

TY  - DATA
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13564
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068
VL  - 23
IS  - 21
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5705
ER  - 

@misc{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068",
volume = "23",
number = "21",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5705"
}

Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068. in International Journal of Molecular Sciences
MDPI., 23(21).
https://hdl.handle.net/21.15107/rcub_cherry_5705

Manojlović-Stojanoski M, Borković-Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068. in International Journal of Molecular Sciences. 2022;23(21).
https://hdl.handle.net/21.15107/rcub_cherry_5705 .

Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068" in International Journal of Molecular Sciences, 23, no. 21 (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5705 .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13438
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.
PB  - Basel : MDPI AG
T2  - International Journal of Molecular Sciences
T1  - The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta
SP  - 13068
VL  - 23
IS  - 21
DO  - 10.3390/ijms232113068
UR  - https://hdl.handle.net/21.15107/rcub_dais_13438
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.",
publisher = "Basel : MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta",
pages = "13068",
volume = "23",
number = "21",
doi = "10.3390/ijms232113068",
url = "https://hdl.handle.net/21.15107/rcub_dais_13438"
}

Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. in International Journal of Molecular Sciences
Basel : MDPI AG., 23(21), 13068.
https://doi.org/10.3390/ijms232113068
https://hdl.handle.net/21.15107/rcub_dais_13438

Manojlović-Stojanoski M, Borković-Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. in International Journal of Molecular Sciences. 2022;23(21):13068.
doi:10.3390/ijms232113068
https://hdl.handle.net/21.15107/rcub_dais_13438 .

Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://doi.org/10.3390/ijms232113068 .,
https://hdl.handle.net/21.15107/rcub_dais_13438 .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13439
AB  - Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - John Wiley and Sons Inc
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
SP  - 1197
EP  - 1206
VL  - 133
IS  - 3
DO  - 10.1111/jam.15638
UR  - https://hdl.handle.net/21.15107/rcub_dais_13439
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2022",
abstract = "Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
pages = "1197-1206",
volume = "133",
number = "3",
doi = "10.1111/jam.15638",
url = "https://hdl.handle.net/21.15107/rcub_dais_13439"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
John Wiley and Sons Inc., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638
https://hdl.handle.net/21.15107/rcub_dais_13439

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković M. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638
https://hdl.handle.net/21.15107/rcub_dais_13439 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 .,
https://hdl.handle.net/21.15107/rcub_dais_13439 .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13439
UR  - https://dais.sanu.ac.rs/123456789/13449
AB  - Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - John Wiley and Sons Inc
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
SP  - 1197
EP  - 1206
VL  - 133
IS  - 3
DO  - 10.1111/jam.15638
UR  - https://hdl.handle.net/21.15107/rcub_dais_13449
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2022",
abstract = "Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
pages = "1197-1206",
volume = "133",
number = "3",
doi = "10.1111/jam.15638",
url = "https://hdl.handle.net/21.15107/rcub_dais_13449"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
John Wiley and Sons Inc., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638
https://hdl.handle.net/21.15107/rcub_dais_13449

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković M. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638
https://hdl.handle.net/21.15107/rcub_dais_13449 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 .,
https://hdl.handle.net/21.15107/rcub_dais_13449 .

TY  - CONF
AU  - Guševac Stojanović, I.
AU  - Drakulić, Dunja
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12359
AB  - Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021
T1  - Does exposure to a single dose of microplastic represent a health risk?
SP  - 102
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_dais_12359
ER  - 

@conference{
author = "Guševac Stojanović, I. and Drakulić, Dunja and Todorović, A. and Filipović, Nenad and Stojanović, Zoran",
year = "2021",
abstract = "Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021",
title = "Does exposure to a single dose of microplastic represent a health risk?",
pages = "102-102",
url = "https://hdl.handle.net/21.15107/rcub_dais_12359"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Filipović, N.,& Stojanović, Z.. (2021). Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021
Belgrade : Society of Physical Chemists of Serbia., 102-102.
https://hdl.handle.net/21.15107/rcub_dais_12359

Guševac Stojanović I, Drakulić D, Todorović A, Filipović N, Stojanović Z. Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021. 2021;:102-102.
https://hdl.handle.net/21.15107/rcub_dais_12359 .

Guševac Stojanović, I., Drakulić, Dunja, Todorović, A., Filipović, Nenad, Stojanović, Zoran, "Does exposure to a single dose of microplastic represent a health risk?" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021 (2021):102-102,
https://hdl.handle.net/21.15107/rcub_dais_12359 .

TY  - CONF
AU  - Guševac Stojanović, I.
AU  - Drakulić, Dunja
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/13619
AB  - Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021
T1  - Does exposure to a single dose of microplastic represent a health risk?
SP  - 572
EP  - 575
UR  - https://hdl.handle.net/21.15107/rcub_dais_13619
ER  - 

@conference{
author = "Guševac Stojanović, I. and Drakulić, Dunja and Todorović, A. and Filipović, Nenad and Stojanović, Zoran",
year = "2021",
abstract = "Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021",
title = "Does exposure to a single dose of microplastic represent a health risk?",
pages = "572-575",
url = "https://hdl.handle.net/21.15107/rcub_dais_13619"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Filipović, N.,& Stojanović, Z.. (2021). Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021
Belgrade : Society of Physical Chemists of Serbia., 572-575.
https://hdl.handle.net/21.15107/rcub_dais_13619

Guševac Stojanović I, Drakulić D, Todorović A, Filipović N, Stojanović Z. Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021. 2021;:572-575.
https://hdl.handle.net/21.15107/rcub_dais_13619 .

Guševac Stojanović, I., Drakulić, Dunja, Todorović, A., Filipović, Nenad, Stojanović, Zoran, "Does exposure to a single dose of microplastic represent a health risk?" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021 (2021):572-575,
https://hdl.handle.net/21.15107/rcub_dais_13619 .

TY  - CONF
AU  - Stojanović, Zoran
AU  - Todorović, Ana
AU  - Martinović, Jelena
AU  - Filipović, Nenad
AU  - Veljković, Filip
AU  - Guševac Stojanović, Ivana
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12381
AB  - Increased plastic usage creates environmental problems due to its accumulation and degradation into microplastic particles (MP), less than 5 mm in diameter. Particles from various sources like food and beverages contaminated with polyethylene terephthalate (PET) could accumulate in different tissues exerting adverse effects. Since data regarding potential acute toxicity in doses that represent realistic/daily ingested MP doses are entirely lacking, male Wistar rats were exposed to a single dose (1.4, 35 or 125 mg/kg) of PET-generated MP by oral gavage. Changes in wet organ weight to body weight ratio of testicles, adrenal glands and kidneys were examined 24 h following the treatment. Results point to increased organ/body weight ratio of testicles in rats exposed to two higher MP doses, while adrenal glands were augmented only after treatment with 125 mg/kg. Further research is needed to gain insight into the underlying mechanisms of the observed phenomenon.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - A single dose of microplastic particles induces changed in organ weight of male Wistar rats
SP  - 119
EP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_dais_12381
ER  - 

@conference{
author = "Stojanović, Zoran and Todorović, Ana and Martinović, Jelena and Filipović, Nenad and Veljković, Filip and Guševac Stojanović, Ivana",
year = "2021",
abstract = "Increased plastic usage creates environmental problems due to its accumulation and degradation into microplastic particles (MP), less than 5 mm in diameter. Particles from various sources like food and beverages contaminated with polyethylene terephthalate (PET) could accumulate in different tissues exerting adverse effects. Since data regarding potential acute toxicity in doses that represent realistic/daily ingested MP doses are entirely lacking, male Wistar rats were exposed to a single dose (1.4, 35 or 125 mg/kg) of PET-generated MP by oral gavage. Changes in wet organ weight to body weight ratio of testicles, adrenal glands and kidneys were examined 24 h following the treatment. Results point to increased organ/body weight ratio of testicles in rats exposed to two higher MP doses, while adrenal glands were augmented only after treatment with 125 mg/kg. Further research is needed to gain insight into the underlying mechanisms of the observed phenomenon.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "A single dose of microplastic particles induces changed in organ weight of male Wistar rats",
pages = "119-122",
url = "https://hdl.handle.net/21.15107/rcub_dais_12381"
}

Stojanović, Z., Todorović, A., Martinović, J., Filipović, N., Veljković, F.,& Guševac Stojanović, I.. (2021). A single dose of microplastic particles induces changed in organ weight of male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 119-122.
https://hdl.handle.net/21.15107/rcub_dais_12381

Stojanović Z, Todorović A, Martinović J, Filipović N, Veljković F, Guševac Stojanović I. A single dose of microplastic particles induces changed in organ weight of male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:119-122.
https://hdl.handle.net/21.15107/rcub_dais_12381 .

Stojanović, Zoran, Todorović, Ana, Martinović, Jelena, Filipović, Nenad, Veljković, Filip, Guševac Stojanović, Ivana, "A single dose of microplastic particles induces changed in organ weight of male Wistar rats" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):119-122,
https://hdl.handle.net/21.15107/rcub_dais_12381 .