Živković, Jelena M.

Link to this page

Authority KeyName Variants
4138e9d6-4fa0-4114-9be6-54cc50295997
  • Živković, Jelena M. (4)
Projects

Author's Bibliography

The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells

Lee, Bill; Gadow, Rainer; Mitić, Vojislav; Najdanović, Jelena; Najman, Stevo; Stojanović, Sanja; Živković, Jelena M.; Ignjatović, Nenad; Uskoković, Dragan; Trajanović, Miroslav

(Paris : Atlantis Press, 2017)

TY  - CONF
AU  - Najdanović, Jelena
AU  - Najman, Stevo
AU  - Stojanović, Sanja
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Trajanović, Miroslav
PY  - 2017
UR  - http://dais.sanu.ac.rs/123456789/2379
AB  - Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.
PB  - Paris : Atlantis Press
C3  - Proceedings of the IV Advanced Ceramics and Applications Conference
T1  - The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells
SP  - 387
EP  - 400
DO  - 10.2991/978-94-6239-213-7_27
UR  - https://hdl.handle.net/21.15107/rcub_dais_2379
ER  - 
@conference{
editor = "Lee, Bill, Gadow, Rainer, Mitić, Vojislav",
author = "Najdanović, Jelena and Najman, Stevo and Stojanović, Sanja and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Trajanović, Miroslav",
year = "2017",
abstract = "Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.",
publisher = "Paris : Atlantis Press",
journal = "Proceedings of the IV Advanced Ceramics and Applications Conference",
title = "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells",
pages = "387-400",
doi = "10.2991/978-94-6239-213-7_27",
url = "https://hdl.handle.net/21.15107/rcub_dais_2379"
}
Lee, B., Gadow, R., Mitić, V., Najdanović, J., Najman, S., Stojanović, S., Živković, J. M., Ignjatović, N., Uskoković, D.,& Trajanović, M.. (2017). The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference
Paris : Atlantis Press., 387-400.
https://doi.org/10.2991/978-94-6239-213-7_27
https://hdl.handle.net/21.15107/rcub_dais_2379
Lee B, Gadow R, Mitić V, Najdanović J, Najman S, Stojanović S, Živković JM, Ignjatović N, Uskoković D, Trajanović M. The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. in Proceedings of the IV Advanced Ceramics and Applications Conference. 2017;:387-400.
doi:10.2991/978-94-6239-213-7_27
https://hdl.handle.net/21.15107/rcub_dais_2379 .
Lee, Bill, Gadow, Rainer, Mitić, Vojislav, Najdanović, Jelena, Najman, Stevo, Stojanović, Sanja, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Trajanović, Miroslav, "The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells" in Proceedings of the IV Advanced Ceramics and Applications Conference (2017):387-400,
https://doi.org/10.2991/978-94-6239-213-7_27 .,
https://hdl.handle.net/21.15107/rcub_dais_2379 .
1

Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles

Najman, Stevo; Petrović, Dragan; Vučković, I.; Živković, Jelena M.; Stojanović, S.; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2013)

TY  - CONF
AU  - Najman, Stevo
AU  - Petrović, Dragan
AU  - Vučković, I.
AU  - Živković, Jelena M.
AU  - Stojanović, S.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/400
AB  - The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles
SP  - 45
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_dais_400
ER  - 
@conference{
author = "Najman, Stevo and Petrović, Dragan and Vučković, I. and Živković, Jelena M. and Stojanović, S. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2013",
abstract = "The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles",
pages = "45-45",
url = "https://hdl.handle.net/21.15107/rcub_dais_400"
}
Najman, S., Petrović, D., Vučković, I., Živković, J. M., Stojanović, S., Ignjatović, N.,& Uskoković, D.. (2013). Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 45-45.
https://hdl.handle.net/21.15107/rcub_dais_400
Najman S, Petrović D, Vučković I, Živković JM, Stojanović S, Ignjatović N, Uskoković D. Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:45-45.
https://hdl.handle.net/21.15107/rcub_dais_400 .
Najman, Stevo, Petrović, Dragan, Vučković, I., Živković, Jelena M., Stojanović, S., Ignjatović, Nenad, Uskoković, Dragan, "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):45-45,
https://hdl.handle.net/21.15107/rcub_dais_400 .

Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid

Vukelić, Marija Đ.; Mitić, Žarko; Miljković, Miroslav; Živković, Jelena M.; Ignjatović, Nenad; Uskoković, Dragan; Živanov Čurlis, Jelena Z.; Vasiljević, Perica; Najman, Stevo

(Milano : Wichtig Publishing, 2012)

TY  - JOUR
AU  - Vukelić, Marija Đ.
AU  - Mitić, Žarko
AU  - Miljković, Miroslav
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Živanov Čurlis, Jelena Z.
AU  - Vasiljević, Perica
AU  - Najman, Stevo
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/489
AB  - Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.
PB  - Milano : Wichtig Publishing
T2  - Journal of Applied Biomaterials and Functional Materials
T1  - Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid
SP  - 43
EP  - 48
DO  - 10.5301/JABFM.2012.9274
UR  - https://hdl.handle.net/21.15107/rcub_dais_489
ER  - 
@article{
author = "Vukelić, Marija Đ. and Mitić, Žarko and Miljković, Miroslav and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan and Živanov Čurlis, Jelena Z. and Vasiljević, Perica and Najman, Stevo",
year = "2012",
abstract = "Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.",
publisher = "Milano : Wichtig Publishing",
journal = "Journal of Applied Biomaterials and Functional Materials",
title = "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid",
pages = "43-48",
doi = "10.5301/JABFM.2012.9274",
url = "https://hdl.handle.net/21.15107/rcub_dais_489"
}
Vukelić, M. Đ., Mitić, Ž., Miljković, M., Živković, J. M., Ignjatović, N., Uskoković, D., Živanov Čurlis, J. Z., Vasiljević, P.,& Najman, S.. (2012). Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials
Milano : Wichtig Publishing., 43-48.
https://doi.org/10.5301/JABFM.2012.9274
https://hdl.handle.net/21.15107/rcub_dais_489
Vukelić MĐ, Mitić Ž, Miljković M, Živković JM, Ignjatović N, Uskoković D, Živanov Čurlis JZ, Vasiljević P, Najman S. Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials. 2012;:43-48.
doi:10.5301/JABFM.2012.9274
https://hdl.handle.net/21.15107/rcub_dais_489 .
Vukelić, Marija Đ., Mitić, Žarko, Miljković, Miroslav, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, Živanov Čurlis, Jelena Z., Vasiljević, Perica, Najman, Stevo, "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid" in Journal of Applied Biomaterials and Functional Materials (2012):43-48,
https://doi.org/10.5301/JABFM.2012.9274 .,
https://hdl.handle.net/21.15107/rcub_dais_489 .
7
6
9

Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid

Vukelić, Marija Đ.; Mitić, Žarko; Miljković, Miroslav; Živković, Jelena M.; Ignjatović, Nenad; Uskoković, Dragan

(Niš : University of Niš, Faculty of Medicine; Department of the Serbian Medical Society Niš, 2011)

TY  - JOUR
AU  - Vukelić, Marija Đ.
AU  - Mitić, Žarko
AU  - Miljković, Miroslav
AU  - Živković, Jelena M.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/701
AB  - The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the integral element of Mammalian bones, the development of the phospate ceramics as potential materials for implantation was enabled. This study investigated whether and in which way biomaterial calcium hydroxyapatite/poly-L-lactide (HAp/PLLA) interacts with the ionic composition of the human plasma. The simulated body fluid (SBF) is an artificial fluid that has the ionic composition and ionic concentration similar to the human blood plasma. HAp/PLLA was incubated for 1, 2, 3 and 5 weeks in SBF. The surfaces of both treated and untreated materials were analyzed on a scanning electron microscopy (SEM), and were also exposed to the energy dispersive X-ray spectroscopy (EDS), while SBF was submitted to the measuring of pH and electrical conductivity. However, our results indicate that the degradational changes of the material HAp/PLLA in SBF start from the surface of the treated material and that observed changes are the consequence of dissolution of its polymer component and the precipitation of the material similar to hydroxyapatite on its surface. This material shows good characteristics that place it among good candidates for the application in orthopedics and maxillofacial surgery.
PB  - Niš : University of Niš, Faculty of Medicine; Department of  the Serbian Medical Society Niš
T2  - Acta medica Medianae
T1  - Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid
SP  - 35
EP  - 39
VL  - 50
IS  - 4
DO  - 10.5633/amm.2011.0406
UR  - https://hdl.handle.net/21.15107/rcub_dais_701
ER  - 
@article{
author = "Vukelić, Marija Đ. and Mitić, Žarko and Miljković, Miroslav and Živković, Jelena M. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2011",
abstract = "The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the integral element of Mammalian bones, the development of the phospate ceramics as potential materials for implantation was enabled. This study investigated whether and in which way biomaterial calcium hydroxyapatite/poly-L-lactide (HAp/PLLA) interacts with the ionic composition of the human plasma. The simulated body fluid (SBF) is an artificial fluid that has the ionic composition and ionic concentration similar to the human blood plasma. HAp/PLLA was incubated for 1, 2, 3 and 5 weeks in SBF. The surfaces of both treated and untreated materials were analyzed on a scanning electron microscopy (SEM), and were also exposed to the energy dispersive X-ray spectroscopy (EDS), while SBF was submitted to the measuring of pH and electrical conductivity. However, our results indicate that the degradational changes of the material HAp/PLLA in SBF start from the surface of the treated material and that observed changes are the consequence of dissolution of its polymer component and the precipitation of the material similar to hydroxyapatite on its surface. This material shows good characteristics that place it among good candidates for the application in orthopedics and maxillofacial surgery.",
publisher = "Niš : University of Niš, Faculty of Medicine; Department of  the Serbian Medical Society Niš",
journal = "Acta medica Medianae",
title = "Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid",
pages = "35-39",
volume = "50",
number = "4",
doi = "10.5633/amm.2011.0406",
url = "https://hdl.handle.net/21.15107/rcub_dais_701"
}
Vukelić, M. Đ., Mitić, Ž., Miljković, M., Živković, J. M., Ignjatović, N.,& Uskoković, D.. (2011). Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid. in Acta medica Medianae
Niš : University of Niš, Faculty of Medicine; Department of  the Serbian Medical Society Niš., 50(4), 35-39.
https://doi.org/10.5633/amm.2011.0406
https://hdl.handle.net/21.15107/rcub_dais_701
Vukelić MĐ, Mitić Ž, Miljković M, Živković JM, Ignjatović N, Uskoković D. Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid. in Acta medica Medianae. 2011;50(4):35-39.
doi:10.5633/amm.2011.0406
https://hdl.handle.net/21.15107/rcub_dais_701 .
Vukelić, Marija Đ., Mitić, Žarko, Miljković, Miroslav, Živković, Jelena M., Ignjatović, Nenad, Uskoković, Dragan, "Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid" in Acta medica Medianae, 50, no. 4 (2011):35-39,
https://doi.org/10.5633/amm.2011.0406 .,
https://hdl.handle.net/21.15107/rcub_dais_701 .
2