TY  - JOUR
AU  - Maksimovic, Milica
AU  - Jovanović, Marina
AU  - Nikolić, Biljana
AU  - Tomić, Nina
AU  - Tenji, Dina
AU  - Stević, Tatjana
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14551
AB  - Persicaria amphibia (subfam. Polygonoideae), an aquatic macrophyte rich in dietary polyphenolics, is used as a traditional remedy and culinary herb. Nevertheless, P. amphibia from the Balkan region has been insufficiently studied and underutilized. Here, the cytotoxicity and antimicrobial properties of the previously chemically characterised ethanol extract of P. amphibia aerial parts were tested. The following methods were carried out: the MTT assay, qRT-PCR, microdilution assay, Chromobacterium violaceum screening assay (monitoring of quorum sensing, QS) and the agar plating method (antifungal activity). The study was conducted to determine the cytotoxic effects of P. amphibia against lung cancer cells (A549) and its combination with cytostatic doxorubicin (Dox). A dose-dependent decrease in cell viability (up to 82% reduction) and additive interactions of the tested agents were noted. Both alone and combined with Dox, P. amphibia reduced the expression of Nrf2 (p < 0.05). In terms of antimicrobial effects, P. amphibia exhibited an antipathogenic effect since it disrupted QS communication, which was evident through the inhibition of violacein production of C. violaceum CV025. The antifungal screening revealed that P. amphibia induced significant growth inhibition of Aspergillus spp. (28.23%). Based on the obtained results, further examination of the potential use of P. amphibia in modern phytotherapy and diet-derived cancer chemoprevention is encouraged.
PB  - Belgrade : 	Institute of Botany and Botanical Garden Jevremovac (University of Belgrade - Faculty of Biology)
T2  - Botanica Serbica
T1  - Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties
SP  - 1
EP  - 8
VL  - 47
IS  - 1
DO  - 10.2298/BOTSERB2301001M
UR  - https://hdl.handle.net/21.15107/rcub_dais_14551
ER  - 

@article{
author = "Maksimovic, Milica and Jovanović, Marina and Nikolić, Biljana and Tomić, Nina and Tenji, Dina and Stević, Tatjana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Persicaria amphibia (subfam. Polygonoideae), an aquatic macrophyte rich in dietary polyphenolics, is used as a traditional remedy and culinary herb. Nevertheless, P. amphibia from the Balkan region has been insufficiently studied and underutilized. Here, the cytotoxicity and antimicrobial properties of the previously chemically characterised ethanol extract of P. amphibia aerial parts were tested. The following methods were carried out: the MTT assay, qRT-PCR, microdilution assay, Chromobacterium violaceum screening assay (monitoring of quorum sensing, QS) and the agar plating method (antifungal activity). The study was conducted to determine the cytotoxic effects of P. amphibia against lung cancer cells (A549) and its combination with cytostatic doxorubicin (Dox). A dose-dependent decrease in cell viability (up to 82% reduction) and additive interactions of the tested agents were noted. Both alone and combined with Dox, P. amphibia reduced the expression of Nrf2 (p < 0.05). In terms of antimicrobial effects, P. amphibia exhibited an antipathogenic effect since it disrupted QS communication, which was evident through the inhibition of violacein production of C. violaceum CV025. The antifungal screening revealed that P. amphibia induced significant growth inhibition of Aspergillus spp. (28.23%). Based on the obtained results, further examination of the potential use of P. amphibia in modern phytotherapy and diet-derived cancer chemoprevention is encouraged.",
publisher = "Belgrade : 	Institute of Botany and Botanical Garden Jevremovac (University of Belgrade - Faculty of Biology)",
journal = "Botanica Serbica",
title = "Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties",
pages = "1-8",
volume = "47",
number = "1",
doi = "10.2298/BOTSERB2301001M",
url = "https://hdl.handle.net/21.15107/rcub_dais_14551"
}

Maksimovic, M., Jovanović, M., Nikolić, B., Tomić, N., Tenji, D., Stević, T.,& Mitić Ćulafić, D.. (2023). Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties. in Botanica Serbica
Belgrade : 	Institute of Botany and Botanical Garden Jevremovac (University of Belgrade - Faculty of Biology)., 47(1), 1-8.
https://doi.org/10.2298/BOTSERB2301001M
https://hdl.handle.net/21.15107/rcub_dais_14551

Maksimovic M, Jovanović M, Nikolić B, Tomić N, Tenji D, Stević T, Mitić Ćulafić D. Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties. in Botanica Serbica. 2023;47(1):1-8.
doi:10.2298/BOTSERB2301001M
https://hdl.handle.net/21.15107/rcub_dais_14551 .

Maksimovic, Milica, Jovanović, Marina, Nikolić, Biljana, Tomić, Nina, Tenji, Dina, Stević, Tatjana, Mitić Ćulafić, Dragana, "Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties" in Botanica Serbica, 47, no. 1 (2023):1-8,
https://doi.org/10.2298/BOTSERB2301001M .,
https://hdl.handle.net/21.15107/rcub_dais_14551 .

TY  - JOUR
AU  - Jovanović, Marina
AU  - Vojvodić, Petar
AU  - Tenji, Dina
AU  - Tomić, Nina
AU  - Nešić, Jovana
AU  - Mitić-Ćulafić, Dragana
AU  - Miočinović, Jelena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14876
AB  - Fermented foods containing psychobiotics are of growing interest among food scientists. Human-derived Limosilactobacillus reuteri DSM 17938, a gut symbiont and potential psychobiotic strain, has been shown to exhibit the following health benefits: anti-inflammation and GABA-production capacity, as well as modulation of pathogen and cancer cell growth. The aim of this research was to develop an acid-coagulated fresh soft quark-type cheese, fermented with L. reuteri DSM 17938, with enhanced bioactivity, sensory acceptability, and overall likeability. Psychobiotic-containing cheeses represent the food of a new generation, so it is of great importance to gain the trust of the consumers. To develop a familiar taste, cheese samples were enriched with mushroom powders of Agaricus bisporus and Pleurotus ostreatus. A high abundance of lactic acid bacteria was maintained in all cheese samples (>log 7.64 CFU/mL), while cheese extracts exhibited cytotoxicity to colon cancer cell line HCT116 (up to 30.96%) in vitro. Additionally, cheese samples provided a favorable medium for the growth of the probiotic Escherichia coli Nissle 1917 (>log 7.11 CFU/mL). Sensory evaluation revealed high scores for all samples (up to 97.21% of maximum overall quality). The survey conducted in this study offered insights into consumer willingness to try products containing psychobiotics. This study demonstrates the potential for the successful development of fermented food products with L. reuteri DSM 17938, which exhibits all the desired traits that consumers may receive well. Further research is required to explore the potential health benefits of these innovative food products.
PB  - Basel : MDPI AG
T2  - Fermentation
T1  - Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability
SP  - 745
VL  - 9
IS  - 8
DO  - 10.3390/fermentation9080745
UR  - https://hdl.handle.net/21.15107/rcub_dais_14876
ER  - 

@article{
author = "Jovanović, Marina and Vojvodić, Petar and Tenji, Dina and Tomić, Nina and Nešić, Jovana and Mitić-Ćulafić, Dragana and Miočinović, Jelena",
year = "2023",
abstract = "Fermented foods containing psychobiotics are of growing interest among food scientists. Human-derived Limosilactobacillus reuteri DSM 17938, a gut symbiont and potential psychobiotic strain, has been shown to exhibit the following health benefits: anti-inflammation and GABA-production capacity, as well as modulation of pathogen and cancer cell growth. The aim of this research was to develop an acid-coagulated fresh soft quark-type cheese, fermented with L. reuteri DSM 17938, with enhanced bioactivity, sensory acceptability, and overall likeability. Psychobiotic-containing cheeses represent the food of a new generation, so it is of great importance to gain the trust of the consumers. To develop a familiar taste, cheese samples were enriched with mushroom powders of Agaricus bisporus and Pleurotus ostreatus. A high abundance of lactic acid bacteria was maintained in all cheese samples (>log 7.64 CFU/mL), while cheese extracts exhibited cytotoxicity to colon cancer cell line HCT116 (up to 30.96%) in vitro. Additionally, cheese samples provided a favorable medium for the growth of the probiotic Escherichia coli Nissle 1917 (>log 7.11 CFU/mL). Sensory evaluation revealed high scores for all samples (up to 97.21% of maximum overall quality). The survey conducted in this study offered insights into consumer willingness to try products containing psychobiotics. This study demonstrates the potential for the successful development of fermented food products with L. reuteri DSM 17938, which exhibits all the desired traits that consumers may receive well. Further research is required to explore the potential health benefits of these innovative food products.",
publisher = "Basel : MDPI AG",
journal = "Fermentation",
title = "Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability",
pages = "745",
volume = "9",
number = "8",
doi = "10.3390/fermentation9080745",
url = "https://hdl.handle.net/21.15107/rcub_dais_14876"
}

Jovanović, M., Vojvodić, P., Tenji, D., Tomić, N., Nešić, J., Mitić-Ćulafić, D.,& Miočinović, J.. (2023). Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability. in Fermentation
Basel : MDPI AG., 9(8), 745.
https://doi.org/10.3390/fermentation9080745
https://hdl.handle.net/21.15107/rcub_dais_14876

Jovanović M, Vojvodić P, Tenji D, Tomić N, Nešić J, Mitić-Ćulafić D, Miočinović J. Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability. in Fermentation. 2023;9(8):745.
doi:10.3390/fermentation9080745
https://hdl.handle.net/21.15107/rcub_dais_14876 .

Jovanović, Marina, Vojvodić, Petar, Tenji, Dina, Tomić, Nina, Nešić, Jovana, Mitić-Ćulafić, Dragana, Miočinović, Jelena, "Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability" in Fermentation, 9, no. 8 (2023):745,
https://doi.org/10.3390/fermentation9080745 .,
https://hdl.handle.net/21.15107/rcub_dais_14876 .