TY  - CONF
AU  - Najman, Stevo
AU  - Petrović, Dragan
AU  - Vučković, I.
AU  - Živković, Jelena M.
AU  - Stojanović, S.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/400
AB  - The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles
SP  - 45
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_dais_400
ER  - 

@conference{
author = "Najman, Stevo and Petrović, Dragan and Vučković, I. and Živković, Jelena M. and Stojanović, S. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2013",
abstract = "The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles",
pages = "45-45",
url = "https://hdl.handle.net/21.15107/rcub_dais_400"
}

Najman, S., Petrović, D., Vučković, I., Živković, J. M., Stojanović, S., Ignjatović, N.,& Uskoković, D.. (2013). Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 45-45.
https://hdl.handle.net/21.15107/rcub_dais_400

Najman S, Petrović D, Vučković I, Živković JM, Stojanović S, Ignjatović N, Uskoković D. Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:45-45.
https://hdl.handle.net/21.15107/rcub_dais_400 .

Najman, Stevo, Petrović, Dragan, Vučković, I., Živković, Jelena M., Stojanović, S., Ignjatović, Nenad, Uskoković, Dragan, "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):45-45,
https://hdl.handle.net/21.15107/rcub_dais_400 .

TY  - CONF
AU  - Stojanović, S.
AU  - Mitić, Ž.
AU  - Najman, Stevo
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/406
AB  - In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions
SP  - 149
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_dais_406
ER  - 

@conference{
author = "Stojanović, S. and Mitić, Ž. and Najman, Stevo and Ignjatović, Nenad and Uskoković, Dragan",
year = "2013",
abstract = "In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions",
pages = "149-149",
url = "https://hdl.handle.net/21.15107/rcub_dais_406"
}

Stojanović, S., Mitić, Ž., Najman, S., Ignjatović, N.,& Uskoković, D.. (2013). Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 149-149.
https://hdl.handle.net/21.15107/rcub_dais_406

Stojanović S, Mitić Ž, Najman S, Ignjatović N, Uskoković D. Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:149-149.
https://hdl.handle.net/21.15107/rcub_dais_406 .

Stojanović, S., Mitić, Ž., Najman, Stevo, Ignjatović, Nenad, Uskoković, Dragan, "Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):149-149,
https://hdl.handle.net/21.15107/rcub_dais_406 .

TY  - CONF
AU  - Stojanović, S.
AU  - Najman, Stevo
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2012
UR  - https://dais.sanu.ac.rs/123456789/458
AB  - The aim of this study was to compare the effects of two types of nanomaterials, cobalt-exchanged hydroxyapatite (CoHAp) and calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA), on the viability of Saos-2 osteoblast-like cells, using MTT test. We have examined the suspension of this materials in the following concentrations: 1.6, 8, 40, 200, 1000 and 2500 μg/ml, as well as extracts in concentration range from 2,5% to 100%. Both materials showed cytotoxic effect at higher concentrations of suspension and extract, respectively, but they were not cytotoxic at lower concentrations. CP/PLGA acted stronger cytotoxic compared to CoHAp, regardless of whether it is examined suspension or extract. CoHAp in small concentrations of suspension and extract acted slightly stimulatory on cells. This suggests that CoHAp may have advantage for use in the in vivo systems.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells
SP  - 126
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_dais_458
ER  - 

@conference{
author = "Stojanović, S. and Najman, Stevo and Ajduković, Zorica and Ignjatović, Nenad and Uskoković, Dragan",
year = "2012",
abstract = "The aim of this study was to compare the effects of two types of nanomaterials, cobalt-exchanged hydroxyapatite (CoHAp) and calcium phosphate/poly-(DL-lactide-co-glycolide) (CP/PLGA), on the viability of Saos-2 osteoblast-like cells, using MTT test. We have examined the suspension of this materials in the following concentrations: 1.6, 8, 40, 200, 1000 and 2500 μg/ml, as well as extracts in concentration range from 2,5% to 100%. Both materials showed cytotoxic effect at higher concentrations of suspension and extract, respectively, but they were not cytotoxic at lower concentrations. CP/PLGA acted stronger cytotoxic compared to CoHAp, regardless of whether it is examined suspension or extract. CoHAp in small concentrations of suspension and extract acted slightly stimulatory on cells. This suggests that CoHAp may have advantage for use in the in vivo systems.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_dais_458"
}

Stojanović, S., Najman, S., Ajduković, Z., Ignjatović, N.,& Uskoković, D.. (2012). Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 126-126.
https://hdl.handle.net/21.15107/rcub_dais_458

Stojanović S, Najman S, Ajduković Z, Ignjatović N, Uskoković D. Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:126-126.
https://hdl.handle.net/21.15107/rcub_dais_458 .

Stojanović, S., Najman, Stevo, Ajduković, Zorica, Ignjatović, Nenad, Uskoković, Dragan, "Comparative study of the effects of different nanomaterials on the viability of human osteoblast-like cells" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):126-126,
https://hdl.handle.net/21.15107/rcub_dais_458 .