TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/653
AB  - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
PB  - IOS Press
T2  - Bio-Medical Materials and Engineering
T1  - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
SP  - 1647
EP  - 1658
VL  - 24
IS  - 4
DO  - 10.3233/BME-140978
UR  - https://hdl.handle.net/21.15107/rcub_dais_653
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2014",
abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.",
publisher = "IOS Press",
journal = "Bio-Medical Materials and Engineering",
title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide",
pages = "1647-1658",
volume = "24",
number = "4",
doi = "10.3233/BME-140978",
url = "https://hdl.handle.net/21.15107/rcub_dais_653"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering
IOS Press., 24(4), 1647-1658.
https://doi.org/10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_653

Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;24(4):1647-1658.
doi:10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_653 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering, 24, no. 4 (2014):1647-1658,
https://doi.org/10.3233/BME-140978 .,
https://hdl.handle.net/21.15107/rcub_dais_653 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/538
AB  - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
T2  - Bio-Medical Materials and Engineering
T1  - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
SP  - 1647
EP  - 1658
VL  - 24
DO  - 10.3233/BME-140978
UR  - https://hdl.handle.net/21.15107/rcub_dais_538
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2014",
abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.",
journal = "Bio-Medical Materials and Engineering",
title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide",
pages = "1647-1658",
volume = "24",
doi = "10.3233/BME-140978",
url = "https://hdl.handle.net/21.15107/rcub_dais_538"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering, 24, 1647-1658.
https://doi.org/10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_538

Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;24:1647-1658.
doi:10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_538 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering, 24 (2014):1647-1658,
https://doi.org/10.3233/BME-140978 .,
https://hdl.handle.net/21.15107/rcub_dais_538 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Uskoković, Dragan
PY  - 2010
UR  - https://dais.sanu.ac.rs/123456789/179
AB  - The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.
PB  - Springer US
T2  - Journal of Materials Science: Materials in Medicine
T1  - A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide
SP  - 231
EP  - 239
VL  - 21
IS  - 1
DO  - 10.1007/s10856-009-3854-6
UR  - https://hdl.handle.net/21.15107/rcub_dais_179
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Uskoković, Dragan",
year = "2010",
abstract = "The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.",
publisher = "Springer US",
journal = "Journal of Materials Science: Materials in Medicine",
title = "A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide",
pages = "231-239",
volume = "21",
number = "1",
doi = "10.1007/s10856-009-3854-6",
url = "https://hdl.handle.net/21.15107/rcub_dais_179"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R.,& Uskoković, D.. (2010). A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. in Journal of Materials Science: Materials in Medicine
Springer US., 21(1), 231-239.
https://doi.org/10.1007/s10856-009-3854-6
https://hdl.handle.net/21.15107/rcub_dais_179

Ignjatović N, Ninkov P, Sabetrasekh R, Uskoković D. A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. in Journal of Materials Science: Materials in Medicine. 2010;21(1):231-239.
doi:10.1007/s10856-009-3854-6
https://hdl.handle.net/21.15107/rcub_dais_179 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Uskoković, Dragan, "A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide" in Journal of Materials Science: Materials in Medicine, 21, no. 1 (2010):231-239,
https://doi.org/10.1007/s10856-009-3854-6 .,
https://hdl.handle.net/21.15107/rcub_dais_179 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2009
UR  - https://dais.sanu.ac.rs/123456789/4932
AB  - Calcium-phosphate/poly(dl-lactide-co-glycolide) (CP/PLGA) composite biomaterial in granular form showed a high potential in the reconstruction of bone tissue. Compared to pure polymers, the combination of CP with biodegradable polymers used in bone drug delivery systems shows certain advantages. Composite biomaterials in nano particulate (NPs) form may have significant advantages over those in micro- or submicro-particulate form. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nano particles contain calcium phosphate coated with bioresorbable polymer and 0.6, 2 and 5wt% tigecycline. The composite was analyzed by FT-IR, XRD, HPLC and AFM methods. The average particle size of the nanocomposite increases with the augmentation of the part of antibiotics, and it ranges from 65 to 95 nm. Release profiles of tigecycline were obtained by UV-VIS spectroscopy in physiological solution at 37oC. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The in vitro cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of LDH activity (under 37%) indicate low cytotoxicity level. Inhibition of bacteria in aerobic and anaerobic conditions in vitro was analyzed after 1, 2 and 3 weeks. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009
T1  - In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_dais_4932
ER  - 

@conference{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2009",
abstract = "Calcium-phosphate/poly(dl-lactide-co-glycolide) (CP/PLGA) composite biomaterial in granular form showed a high potential in the reconstruction of bone tissue. Compared to pure polymers, the combination of CP with biodegradable polymers used in bone drug delivery systems shows certain advantages. Composite biomaterials in nano particulate (NPs) form may have significant advantages over those in micro- or submicro-particulate form. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nano particles contain calcium phosphate coated with bioresorbable polymer and 0.6, 2 and 5wt% tigecycline. The composite was analyzed by FT-IR, XRD, HPLC and AFM methods. The average particle size of the nanocomposite increases with the augmentation of the part of antibiotics, and it ranges from 65 to 95 nm. Release profiles of tigecycline were obtained by UV-VIS spectroscopy in physiological solution at 37oC. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The in vitro cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of LDH activity (under 37%) indicate low cytotoxicity level. Inhibition of bacteria in aerobic and anaerobic conditions in vitro was analyzed after 1, 2 and 3 weeks. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009",
title = "In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_dais_4932"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2009). In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline. in Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009
Belgrade : Institute of Technical Sciences of SASA., 71-71.
https://hdl.handle.net/21.15107/rcub_dais_4932

Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline. in Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009. 2009;:71-71.
https://hdl.handle.net/21.15107/rcub_dais_4932 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline" in Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009 (2009):71-71,
https://hdl.handle.net/21.15107/rcub_dais_4932 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Ajduković, Zorica
AU  - Vasiljević Radović, Dana
AU  - Uskoković Dragan
PY  - 2007
UR  - https://dais.sanu.ac.rs/123456789/15266
AB  - A calcium phosphate/poly-d,l-lactide-co-glycolide (BCP/DLPLG) composite biomaterial was synthesized in the shape of spherical granules (150–200 ␮m) and nanostructured particles (30–40 nm) by an emulsification/evaporation procedure. Calcium phosphate components were coated with amorphous polymer phase. The influence of the processing technique on the structure and characteristics of the BCP/DLPLG composite biomaterial was studied by X-ray diffraction analysis (XRD), differential scanning calorimetry (DSC), scanning electronic microscopy (SEM), and atomic force microscopy (AFM). In vitro cytotoxicity research was conducted on cellular cultures of fibroblasts of animals. The possibility of BCP/DLPLG application was examined by its use in reparation of lost bone tissue of patients.
PB  - Elsevier
T2  - Journal of the European Ceramic Society
T1  - Biphasic calcium phosphate coated with poly-d,l-lactide-co-glycolide biomaterial as a bone substitute
SP  - 1589
EP  - 1594
VL  - 27
IS  - 2-3
DO  - 10.1016/j.jeurceramsoc.2006.04.104
UR  - https://hdl.handle.net/21.15107/rcub_dais_15266
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Ajduković, Zorica and Vasiljević Radović, Dana and Uskoković Dragan",
year = "2007",
abstract = "A calcium phosphate/poly-d,l-lactide-co-glycolide (BCP/DLPLG) composite biomaterial was synthesized in the shape of spherical granules (150–200 ␮m) and nanostructured particles (30–40 nm) by an emulsification/evaporation procedure. Calcium phosphate components were coated with amorphous polymer phase. The influence of the processing technique on the structure and characteristics of the BCP/DLPLG composite biomaterial was studied by X-ray diffraction analysis (XRD), differential scanning calorimetry (DSC), scanning electronic microscopy (SEM), and atomic force microscopy (AFM). In vitro cytotoxicity research was conducted on cellular cultures of fibroblasts of animals. The possibility of BCP/DLPLG application was examined by its use in reparation of lost bone tissue of patients.",
publisher = "Elsevier",
journal = "Journal of the European Ceramic Society",
title = "Biphasic calcium phosphate coated with poly-d,l-lactide-co-glycolide biomaterial as a bone substitute",
pages = "1589-1594",
volume = "27",
number = "2-3",
doi = "10.1016/j.jeurceramsoc.2006.04.104",
url = "https://hdl.handle.net/21.15107/rcub_dais_15266"
}

Ignjatović, N., Ninkov, P., Ajduković, Z., Vasiljević Radović, D.,& Uskoković Dragan. (2007). Biphasic calcium phosphate coated with poly-d,l-lactide-co-glycolide biomaterial as a bone substitute. in Journal of the European Ceramic Society
Elsevier., 27(2-3), 1589-1594.
https://doi.org/10.1016/j.jeurceramsoc.2006.04.104
https://hdl.handle.net/21.15107/rcub_dais_15266

Ignjatović N, Ninkov P, Ajduković Z, Vasiljević Radović D, Uskoković Dragan. Biphasic calcium phosphate coated with poly-d,l-lactide-co-glycolide biomaterial as a bone substitute. in Journal of the European Ceramic Society. 2007;27(2-3):1589-1594.
doi:10.1016/j.jeurceramsoc.2006.04.104
https://hdl.handle.net/21.15107/rcub_dais_15266 .

Ignjatović, Nenad, Ninkov, Petar, Ajduković, Zorica, Vasiljević Radović, Dana, Uskoković Dragan, "Biphasic calcium phosphate coated with poly-d,l-lactide-co-glycolide biomaterial as a bone substitute" in Journal of the European Ceramic Society, 27, no. 2-3 (2007):1589-1594,
https://doi.org/10.1016/j.jeurceramsoc.2006.04.104 .,
https://hdl.handle.net/21.15107/rcub_dais_15266 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Kojić, Vesna
AU  - Bokurov, Miloš
AU  - Srdić, Vladimir
AU  - Krnojelac, Dijana
AU  - Selaković, Srećko
AU  - Uskoković, Dragan
PY  - 2006
UR  - https://dais.sanu.ac.rs/123456789/14367
AB  - Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).
PB  - Wiley
T2  - Microscopy Research and Technique
T1  - Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials
SP  - 976
EP  - 982
VL  - 69
IS  - 12
DO  - 10.1002/jemt.20374
UR  - https://hdl.handle.net/21.15107/rcub_dais_14367
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Kojić, Vesna and Bokurov, Miloš and Srdić, Vladimir and Krnojelac, Dijana and Selaković, Srećko and Uskoković, Dragan",
year = "2006",
abstract = "Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).",
publisher = "Wiley",
journal = "Microscopy Research and Technique",
title = "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials",
pages = "976-982",
volume = "69",
number = "12",
doi = "10.1002/jemt.20374",
url = "https://hdl.handle.net/21.15107/rcub_dais_14367"
}

Ignjatović, N., Ninkov, P., Kojić, V., Bokurov, M., Srdić, V., Krnojelac, D., Selaković, S.,& Uskoković, D.. (2006). Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. in Microscopy Research and Technique
Wiley., 69(12), 976-982.
https://doi.org/10.1002/jemt.20374
https://hdl.handle.net/21.15107/rcub_dais_14367

Ignjatović N, Ninkov P, Kojić V, Bokurov M, Srdić V, Krnojelac D, Selaković S, Uskoković D. Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. in Microscopy Research and Technique. 2006;69(12):976-982.
doi:10.1002/jemt.20374
https://hdl.handle.net/21.15107/rcub_dais_14367 .

Ignjatović, Nenad, Ninkov, Petar, Kojić, Vesna, Bokurov, Miloš, Srdić, Vladimir, Krnojelac, Dijana, Selaković, Srećko, Uskoković, Dragan, "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials" in Microscopy Research and Technique, 69, no. 12 (2006):976-982,
https://doi.org/10.1002/jemt.20374 .,
https://hdl.handle.net/21.15107/rcub_dais_14367 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Ajduković, Zorica
AU  - Konstantinović, Vitomir
AU  - Uskoković, Dragan
PY  - 2005
UR  - https://dais.sanu.ac.rs/123456789/8952
AB  - Composite biomaterials, like calciumphosphate/bioresorbable polymer, offer excellent potential for reconstruction and reparation of bone tissue defects induced by different sources. In this paper synthesis of calciumphosphate/poly-DL-lactide-co-glycolide (BCP/DLPLG) composite biomaterial formed as filler and blocks was studied. BCP/DLPLG composite biomaterial was produced in the form of spherical granules of BCP covered by a DLPLG layer, average diameter of 150-250 mu m. By cold and hot pressing of granules at up to 10000 kg/cm(2), blocks with fine distribution of phases and porosity up to 3% were obtained. Characterization was performed by wide-angle X-ray structural analysis (WAXS), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), infrared spectroscopy (IR), and mechanical properties by defining the compressive strength. In vitro citotoxicity research was carried out on cellular cultures of fibroblasts of human (MRC5) and mouse (L929). In vivo research was performed in two steps. Reparatory ability of BCP/DLPLG in mice was examined in the first step, and then bone tissue reconstruction possibilities on 10 patients in the next step. In vitro tests showed very good fibroblast adhesion and non-citotoxicity of the composite. A material is considered non-cytotoxic if the cell survival is above 50%, and in our case it was 90%. In vivo research on mice indicated high level of reparatory ability of this composite with formation of new bone and vascular tissue six weeks after reparation. Application of this composite for healing infrabone defects of patients showed a high level of osseous regeneration.
PB  - Durnten-Zurich : Trans Tech Publications Ltd.
T2  - Current Research in Advanced Materials & Processes
T1  - Biphasic calcium phosphate/poly-(DL-lactide-co-glycolide) biocomposite as filler and blocks for reparation of bone tissue
SP  - 519
EP  - 524
DO  - 10.4028/www.scientific.net/MSF.494.519
UR  - https://hdl.handle.net/21.15107/rcub_dais_8952
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Ajduković, Zorica and Konstantinović, Vitomir and Uskoković, Dragan",
year = "2005",
abstract = "Composite biomaterials, like calciumphosphate/bioresorbable polymer, offer excellent potential for reconstruction and reparation of bone tissue defects induced by different sources. In this paper synthesis of calciumphosphate/poly-DL-lactide-co-glycolide (BCP/DLPLG) composite biomaterial formed as filler and blocks was studied. BCP/DLPLG composite biomaterial was produced in the form of spherical granules of BCP covered by a DLPLG layer, average diameter of 150-250 mu m. By cold and hot pressing of granules at up to 10000 kg/cm(2), blocks with fine distribution of phases and porosity up to 3% were obtained. Characterization was performed by wide-angle X-ray structural analysis (WAXS), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), infrared spectroscopy (IR), and mechanical properties by defining the compressive strength. In vitro citotoxicity research was carried out on cellular cultures of fibroblasts of human (MRC5) and mouse (L929). In vivo research was performed in two steps. Reparatory ability of BCP/DLPLG in mice was examined in the first step, and then bone tissue reconstruction possibilities on 10 patients in the next step. In vitro tests showed very good fibroblast adhesion and non-citotoxicity of the composite. A material is considered non-cytotoxic if the cell survival is above 50%, and in our case it was 90%. In vivo research on mice indicated high level of reparatory ability of this composite with formation of new bone and vascular tissue six weeks after reparation. Application of this composite for healing infrabone defects of patients showed a high level of osseous regeneration.",
publisher = "Durnten-Zurich : Trans Tech Publications Ltd.",
journal = "Current Research in Advanced Materials & Processes",
title = "Biphasic calcium phosphate/poly-(DL-lactide-co-glycolide) biocomposite as filler and blocks for reparation of bone tissue",
pages = "519-524",
doi = "10.4028/www.scientific.net/MSF.494.519",
url = "https://hdl.handle.net/21.15107/rcub_dais_8952"
}

Ignjatović, N., Ninkov, P., Ajduković, Z., Konstantinović, V.,& Uskoković, D.. (2005). Biphasic calcium phosphate/poly-(DL-lactide-co-glycolide) biocomposite as filler and blocks for reparation of bone tissue. in Current Research in Advanced Materials & Processes
Durnten-Zurich : Trans Tech Publications Ltd.., 519-524.
https://doi.org/10.4028/www.scientific.net/MSF.494.519
https://hdl.handle.net/21.15107/rcub_dais_8952

Ignjatović N, Ninkov P, Ajduković Z, Konstantinović V, Uskoković D. Biphasic calcium phosphate/poly-(DL-lactide-co-glycolide) biocomposite as filler and blocks for reparation of bone tissue. in Current Research in Advanced Materials & Processes. 2005;:519-524.
doi:10.4028/www.scientific.net/MSF.494.519
https://hdl.handle.net/21.15107/rcub_dais_8952 .

Ignjatović, Nenad, Ninkov, Petar, Ajduković, Zorica, Konstantinović, Vitomir, Uskoković, Dragan, "Biphasic calcium phosphate/poly-(DL-lactide-co-glycolide) biocomposite as filler and blocks for reparation of bone tissue" in Current Research in Advanced Materials & Processes (2005):519-524,
https://doi.org/10.4028/www.scientific.net/MSF.494.519 .,
https://hdl.handle.net/21.15107/rcub_dais_8952 .