TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Tatalović, N.
AU  - Veljković, F.
AU  - Martinović, J.
AU  - Zarić Kontić, M.
AU  - Blagojević, D.
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://www.ecnp.eu/congress2022/ECNPcongress/programme/programme#!abstractdetails/0000506150
UR  - https://dais.sanu.ac.rs/123456789/13626
AB  - Introduction: Cerebral hypoperfusion (CH), a reduced cerebral blood flow, characteristic for aging and neurodegenerative disorders, might be associated with a pro-oxidative state in the brain due to the generation of reactive oxygen species (ROS) that cause neuronal damage. These overgenerated pro-oxidants might be reduced, disabled and neutralized by the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). A sex steroid hormone, progesterone, in several experimental models of neuronal injury exerts anti-oxidative properties upregulating anti-oxidative molecules and thus promoting neuroprotection. However, its efficacy in the state of CH is still unknown and it is necessary to better understand the mechanism underlying this hormone’s neuroprotective effects.
Aim: We investigated whether progesterone is able to modulate CH-induced pro-oxidative/anti-oxidative imbalance, as well as SOD and CAT activities, in the prefrontal cortex of three-month-old male Wistar rats subjected to permanent bilateral common carotid artery occlusion (2VO).
Methods: The appropriate actions were undertaken to reduce the distress of the animals in agreement with the European Community Council Directive of 86/609/EEC and 010/63/EU for animal experiments. All experimental procedures were allowed by the Ethical Committee for the Use of Laboratory Animals of VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Republic of Serbia (protocol authorization numbers 02/11 and 323-07-04253/2016-05). Following chloral hydrate anaesthesia, 8 rats randomly assigned in 2VO groups were double occluded with 5–0 silk suture, while 4 animals from sham operated group were subjected to the same surgical procedure but without actual ligation of carotid arteries. Four hours following the surgical procedure animals from sham operated group, used as controls, were subcutaneously treated with vehicle (commercial flax oil, 1 mg/kg/day, S + V), while animals in 2VO groups were subjected to either progesterone (1.7 mg/kg/day, 2VO + P) or vehicle (commercial flax oil, 1 mg/kg/day, 2VO + V). Following 7 days lasting treatments, pro-oxidative/anti-oxidative balance was assessed according to the method of Alamdari et al. [1]; while activities of anti-oxidative enzymes were determined as described in the study by Tatalović et al. [2]. One-way analysis of variance (ANOVA) test followed by Tukey’s post hoc test was employed to analyse data. The values of p < 0.05 or less were considered statistically significant.
Results: Obtained results demonstrate that compared to controls, 2VO and vehicle treatment induced a significant increase of pro-oxidants levels (p < 0.01) as well as the activities of both investigated anti-oxidative enzymes, although only the CAT activity was significantly increased (p < 0.05). Following progesterone treatment, pro-oxidants levels were lowered to the control level which caused the difference between the 2VO groups (p < 0.05). In parallel, no statistically significant change in SOD activity was observed, while CAT was upregulated (p < 0.05).
Conclusion: Presented results suggest that in the prefrontal cortex in the state of CH, progesterone, by modulating the CAT activity, downregulates overgenerated reactive species, reduces CH-induced oxidative stress and exerts anti-oxidative outcomes.
PB  - ECNP
C3  - 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
T1  - Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model
SP  - P.0477
UR  - https://hdl.handle.net/21.15107/rcub_dais_13626
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Tatalović, N. and Veljković, F. and Martinović, J. and Zarić Kontić, M. and Blagojević, D. and Stojanović, Zoran and Drakulić, Dunja",
year = "2022",
abstract = "Introduction: Cerebral hypoperfusion (CH), a reduced cerebral blood flow, characteristic for aging and neurodegenerative disorders, might be associated with a pro-oxidative state in the brain due to the generation of reactive oxygen species (ROS) that cause neuronal damage. These overgenerated pro-oxidants might be reduced, disabled and neutralized by the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). A sex steroid hormone, progesterone, in several experimental models of neuronal injury exerts anti-oxidative properties upregulating anti-oxidative molecules and thus promoting neuroprotection. However, its efficacy in the state of CH is still unknown and it is necessary to better understand the mechanism underlying this hormone’s neuroprotective effects.
Aim: We investigated whether progesterone is able to modulate CH-induced pro-oxidative/anti-oxidative imbalance, as well as SOD and CAT activities, in the prefrontal cortex of three-month-old male Wistar rats subjected to permanent bilateral common carotid artery occlusion (2VO).
Methods: The appropriate actions were undertaken to reduce the distress of the animals in agreement with the European Community Council Directive of 86/609/EEC and 010/63/EU for animal experiments. All experimental procedures were allowed by the Ethical Committee for the Use of Laboratory Animals of VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Republic of Serbia (protocol authorization numbers 02/11 and 323-07-04253/2016-05). Following chloral hydrate anaesthesia, 8 rats randomly assigned in 2VO groups were double occluded with 5–0 silk suture, while 4 animals from sham operated group were subjected to the same surgical procedure but without actual ligation of carotid arteries. Four hours following the surgical procedure animals from sham operated group, used as controls, were subcutaneously treated with vehicle (commercial flax oil, 1 mg/kg/day, S + V), while animals in 2VO groups were subjected to either progesterone (1.7 mg/kg/day, 2VO + P) or vehicle (commercial flax oil, 1 mg/kg/day, 2VO + V). Following 7 days lasting treatments, pro-oxidative/anti-oxidative balance was assessed according to the method of Alamdari et al. [1]; while activities of anti-oxidative enzymes were determined as described in the study by Tatalović et al. [2]. One-way analysis of variance (ANOVA) test followed by Tukey’s post hoc test was employed to analyse data. The values of p < 0.05 or less were considered statistically significant.
Results: Obtained results demonstrate that compared to controls, 2VO and vehicle treatment induced a significant increase of pro-oxidants levels (p < 0.01) as well as the activities of both investigated anti-oxidative enzymes, although only the CAT activity was significantly increased (p < 0.05). Following progesterone treatment, pro-oxidants levels were lowered to the control level which caused the difference between the 2VO groups (p < 0.05). In parallel, no statistically significant change in SOD activity was observed, while CAT was upregulated (p < 0.05).
Conclusion: Presented results suggest that in the prefrontal cortex in the state of CH, progesterone, by modulating the CAT activity, downregulates overgenerated reactive species, reduces CH-induced oxidative stress and exerts anti-oxidative outcomes.",
publisher = "ECNP",
journal = "35the ECNP Congress, 15-18 October 2022, Vienna, Austria",
title = "Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model",
pages = "P.0477",
url = "https://hdl.handle.net/21.15107/rcub_dais_13626"
}

Guševac Stojanović, I., Tatalović, N., Veljković, F., Martinović, J., Zarić Kontić, M., Blagojević, D., Stojanović, Z.,& Drakulić, D.. (2022). Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
ECNP., P.0477.
https://hdl.handle.net/21.15107/rcub_dais_13626

Guševac Stojanović I, Tatalović N, Veljković F, Martinović J, Zarić Kontić M, Blagojević D, Stojanović Z, Drakulić D. Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria. 2022;:P.0477.
https://hdl.handle.net/21.15107/rcub_dais_13626 .

Guševac Stojanović, Ivana, Tatalović, N., Veljković, F., Martinović, J., Zarić Kontić, M., Blagojević, D., Stojanović, Zoran, Drakulić, Dunja, "Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model" in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria (2022):P.0477,
https://hdl.handle.net/21.15107/rcub_dais_13626 .

TY  - CONF
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
AU  - Veljković, F.
AU  - Martinović, J.
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Guševac Stojanović, Ivana
PY  - 2022
UR  - https://www.ecnp.eu/congress2022/ECNPcongress/programme/programme#!abstractdetails/0000505040
UR  - https://dais.sanu.ac.rs/123456789/13625
AB  - Introduction: As a result of human activity, the amount of plastic waste has increased immensely in the environment. While the main concern has been orientated toward visible litter, more recently fragments of plastic less than 5 mm in diameter, called microplastic particles (MP), raised the attention due to their ability to induce toxic effects in organisms. The toxicity of MP increases with a decrease in size since smaller sized particles are absorbed systematically and accumulated in different organs/tissues, including brain.

Aim: Present study is designed to compare possible toxic acute effects of different sized MP, originating from polyethylene-terephthalate bottles, on toxicity signs, sensory-motor functions and relative brain weight in young male Wistar rats.

Methods: Rats (n = 24) were divided in four experimental groups: (I) intact (controls); Q that orally received a 2.5 ml of Milli-Q; P1/P2 that were respectively orally administrated with 35 mg/kg of MP in median diameter of either 40 or 70 μm dissolved in a 2.5 ml of Milli-Q. 24 h after treatments, using toxicity clinical test, we assessed the agitation, convulsion, piloerection, sleepiness and lethargy. Parameters were valued as: – (no effect), + (mild effect), ++ (moderate effect) and +++ (major effect). Then, we evaluated sensory-motor functions by monitoring: consciousness (scored 0/1 point), respiration (scored 0/1), spontaneous activity (scored 0/1/2/3), forepaw outstretching (scored 0/1/2), climbing (scored 0/1), visual placing (scored 0/1), cage grasp (scored 0/1), gait posture (scored 0/1/2), geotaxis (scored 0/1), hearing (scored 0/1) and pacing/circling (scored 0/1). Total score was expressed as the sum of average scores of all investigated parameters and graded on the scale from 0 to 15. Then, rats were weighed and sacrificed, while their brains were carefully removed and weighed. The relative brain weights were calculated as the ratio of brain (wet tissue, mg) weight to body weight (g). For toxicity clinical testing a descriptive analysis was used while other data were analysed by One-way analysis of variance followed by Tukey’s posthoc test. Results were considered significant at p < 0.05.
Results: Clinical toxicity signs were similar between groups while results of sensory-motor test point to unaltered sensory-motor functions upon MP treatments compared to controls (F (3, 20) = 0.9538; P = 0.4336). The absence of changes might be attributed to acute treatment, brief evaluation period and lack of cumulative effect upon MP treatments. Nonetheless, only smaller sized MP led to an increase in relative brain weight compared to controls (F (3, 20) = 3.425; P = 0.0369; p < 0.05) which might indicate the capability of smaller sized MP to cross blood-brain barrier and be accumulated in the brain inducing oxidative stress and inflammatory response. Although larger sized MP did not cause changes in relative brain weight, the possible modulations of additional parameters that were not the focus of this study should not be omitted.
Conclusion: According to the presented results, even a smaller sized MP applied in a single dose could be harmful to health, thus, greater control of plastic usage and disposal is essential for humanity.
PB  - ECNP
C3  - 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
T1  - Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats
SP  - P.0244
UR  - https://hdl.handle.net/21.15107/rcub_dais_13625
ER  - 

@conference{
author = "Stojanović, Zoran and Drakulić, Dunja and Veljković, F. and Martinović, J. and Todorović, A. and Filipović, Nenad and Stevanović, Magdalena and Guševac Stojanović, Ivana",
year = "2022",
abstract = "Introduction: As a result of human activity, the amount of plastic waste has increased immensely in the environment. While the main concern has been orientated toward visible litter, more recently fragments of plastic less than 5 mm in diameter, called microplastic particles (MP), raised the attention due to their ability to induce toxic effects in organisms. The toxicity of MP increases with a decrease in size since smaller sized particles are absorbed systematically and accumulated in different organs/tissues, including brain.

Aim: Present study is designed to compare possible toxic acute effects of different sized MP, originating from polyethylene-terephthalate bottles, on toxicity signs, sensory-motor functions and relative brain weight in young male Wistar rats.

Methods: Rats (n = 24) were divided in four experimental groups: (I) intact (controls); Q that orally received a 2.5 ml of Milli-Q; P1/P2 that were respectively orally administrated with 35 mg/kg of MP in median diameter of either 40 or 70 μm dissolved in a 2.5 ml of Milli-Q. 24 h after treatments, using toxicity clinical test, we assessed the agitation, convulsion, piloerection, sleepiness and lethargy. Parameters were valued as: – (no effect), + (mild effect), ++ (moderate effect) and +++ (major effect). Then, we evaluated sensory-motor functions by monitoring: consciousness (scored 0/1 point), respiration (scored 0/1), spontaneous activity (scored 0/1/2/3), forepaw outstretching (scored 0/1/2), climbing (scored 0/1), visual placing (scored 0/1), cage grasp (scored 0/1), gait posture (scored 0/1/2), geotaxis (scored 0/1), hearing (scored 0/1) and pacing/circling (scored 0/1). Total score was expressed as the sum of average scores of all investigated parameters and graded on the scale from 0 to 15. Then, rats were weighed and sacrificed, while their brains were carefully removed and weighed. The relative brain weights were calculated as the ratio of brain (wet tissue, mg) weight to body weight (g). For toxicity clinical testing a descriptive analysis was used while other data were analysed by One-way analysis of variance followed by Tukey’s posthoc test. Results were considered significant at p < 0.05.
Results: Clinical toxicity signs were similar between groups while results of sensory-motor test point to unaltered sensory-motor functions upon MP treatments compared to controls (F (3, 20) = 0.9538; P = 0.4336). The absence of changes might be attributed to acute treatment, brief evaluation period and lack of cumulative effect upon MP treatments. Nonetheless, only smaller sized MP led to an increase in relative brain weight compared to controls (F (3, 20) = 3.425; P = 0.0369; p < 0.05) which might indicate the capability of smaller sized MP to cross blood-brain barrier and be accumulated in the brain inducing oxidative stress and inflammatory response. Although larger sized MP did not cause changes in relative brain weight, the possible modulations of additional parameters that were not the focus of this study should not be omitted.
Conclusion: According to the presented results, even a smaller sized MP applied in a single dose could be harmful to health, thus, greater control of plastic usage and disposal is essential for humanity.",
publisher = "ECNP",
journal = "35the ECNP Congress, 15-18 October 2022, Vienna, Austria",
title = "Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats",
pages = "P.0244",
url = "https://hdl.handle.net/21.15107/rcub_dais_13625"
}

Stojanović, Z., Drakulić, D., Veljković, F., Martinović, J., Todorović, A., Filipović, N., Stevanović, M.,& Guševac Stojanović, I.. (2022). Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
ECNP., P.0244.
https://hdl.handle.net/21.15107/rcub_dais_13625

Stojanović Z, Drakulić D, Veljković F, Martinović J, Todorović A, Filipović N, Stevanović M, Guševac Stojanović I. Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria. 2022;:P.0244.
https://hdl.handle.net/21.15107/rcub_dais_13625 .

Stojanović, Zoran, Drakulić, Dunja, Veljković, F., Martinović, J., Todorović, A., Filipović, Nenad, Stevanović, Magdalena, Guševac Stojanović, Ivana, "Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats" in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria (2022):P.0244,
https://hdl.handle.net/21.15107/rcub_dais_13625 .

TY  - CONF
AU  - Todorović, A.
AU  - Pavlović, I.
AU  - Pejić, S.
AU  - Miletić Vukajlović, J.
AU  - Veljković, F.
AU  - Filipović Tričković, J.
AU  - Valenta Šobot, A.
AU  - Martinović, J.
AU  - Guševac Stojanović, I.
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12379
AB  - Flaxseed oil, extracted from ripened flaxseeds, is functional food source that is associated with health benefits in many diseases, including cardiovascular,
cancer, osteoporosis, etc. Besides saturated fatty acids and monounsaturated fatty acids, it also contains easily oxidized polyunsaturated fatty acids of the n-3 family. In current study, we tested the effects of commercial flaxseed oil in rat liver by measuring parameters related to free radical challenge (pro/antioxidant balance (PAB), lipid peroxidation (LPO) products and glutathione (GSH) level). Overall, applied treatment maintains general prooxidant load and antioxidant capacity since the level of PAB was unchanged. However, it exerts significant modulatory effect on particular redox parameters by increasing the amount of LPO products and consequently the susceptibility of tissue to free radical oxidative damage, which challenges the antioxidant defence system that
in return elevates GSH concentration.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - Modulation of redox parameters in rat liver induced by flaxseed oil
SP  - 180
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_dais_12379
ER  - 

@conference{
author = "Todorović, A. and Pavlović, I. and Pejić, S. and Miletić Vukajlović, J. and Veljković, F. and Filipović Tričković, J. and Valenta Šobot, A. and Martinović, J. and Guševac Stojanović, I. and Stojanović, Zoran and Drakulić, Dunja",
year = "2021",
abstract = "Flaxseed oil, extracted from ripened flaxseeds, is functional food source that is associated with health benefits in many diseases, including cardiovascular,
cancer, osteoporosis, etc. Besides saturated fatty acids and monounsaturated fatty acids, it also contains easily oxidized polyunsaturated fatty acids of the n-3 family. In current study, we tested the effects of commercial flaxseed oil in rat liver by measuring parameters related to free radical challenge (pro/antioxidant balance (PAB), lipid peroxidation (LPO) products and glutathione (GSH) level). Overall, applied treatment maintains general prooxidant load and antioxidant capacity since the level of PAB was unchanged. However, it exerts significant modulatory effect on particular redox parameters by increasing the amount of LPO products and consequently the susceptibility of tissue to free radical oxidative damage, which challenges the antioxidant defence system that
in return elevates GSH concentration.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "Modulation of redox parameters in rat liver induced by flaxseed oil",
pages = "180-183",
url = "https://hdl.handle.net/21.15107/rcub_dais_12379"
}

Todorović, A., Pavlović, I., Pejić, S., Miletić Vukajlović, J., Veljković, F., Filipović Tričković, J., Valenta Šobot, A., Martinović, J., Guševac Stojanović, I., Stojanović, Z.,& Drakulić, D.. (2021). Modulation of redox parameters in rat liver induced by flaxseed oil. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 180-183.
https://hdl.handle.net/21.15107/rcub_dais_12379

Todorović A, Pavlović I, Pejić S, Miletić Vukajlović J, Veljković F, Filipović Tričković J, Valenta Šobot A, Martinović J, Guševac Stojanović I, Stojanović Z, Drakulić D. Modulation of redox parameters in rat liver induced by flaxseed oil. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:180-183.
https://hdl.handle.net/21.15107/rcub_dais_12379 .

Todorović, A., Pavlović, I., Pejić, S., Miletić Vukajlović, J., Veljković, F., Filipović Tričković, J., Valenta Šobot, A., Martinović, J., Guševac Stojanović, I., Stojanović, Zoran, Drakulić, Dunja, "Modulation of redox parameters in rat liver induced by flaxseed oil" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):180-183,
https://hdl.handle.net/21.15107/rcub_dais_12379 .

TY  - CONF
AU  - Martinović, J.
AU  - Guševac Stojanović, I.
AU  - Zarić, M
AU  - Todorović, A.
AU  - Veljković, F.
AU  - Pejić, S.
AU  - Stojanović, Zoran
AU  - Mitrović, N.
AU  - Grković, I.
AU  - Drakulić, Dunja
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12378
AB  - D-galactose (d-gal) is an important physiological nutrient. According to the
widely accepted aging metabolic theory d-gal at high levels can be converted
into aldose and hydroperoxide, resulting in the overproduction of reactive
oxygen species (ROS). Increased ROS levels may subsequently cause oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis which are hallmarks of natural senescence as well as various pathological conditions. We investigated the effects of chronic oral d-gal intake (200 mg/kg and 500 mg/kg for 6 weeks) on physiological, neurological and toxicity parameters in 3 months old male Wistar rats. The obtained results indicate that body weight, food intake, serum glucose, neurological and toxicity status remained unaffected while urine proteins were significantly increased in d-gal treated rats. Although there was no effect on the general health status of the animals, our findings suggest that chronic oral d-gal administration may lead to renal dysfunction.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - Effects of chronic oral D-galactose treatment on general health status in male Wistar rats
SP  - 115
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_dais_12378
ER  - 

@conference{
author = "Martinović, J. and Guševac Stojanović, I. and Zarić, M and Todorović, A. and Veljković, F. and Pejić, S. and Stojanović, Zoran and Mitrović, N. and Grković, I. and Drakulić, Dunja",
year = "2021",
abstract = "D-galactose (d-gal) is an important physiological nutrient. According to the
widely accepted aging metabolic theory d-gal at high levels can be converted
into aldose and hydroperoxide, resulting in the overproduction of reactive
oxygen species (ROS). Increased ROS levels may subsequently cause oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis which are hallmarks of natural senescence as well as various pathological conditions. We investigated the effects of chronic oral d-gal intake (200 mg/kg and 500 mg/kg for 6 weeks) on physiological, neurological and toxicity parameters in 3 months old male Wistar rats. The obtained results indicate that body weight, food intake, serum glucose, neurological and toxicity status remained unaffected while urine proteins were significantly increased in d-gal treated rats. Although there was no effect on the general health status of the animals, our findings suggest that chronic oral d-gal administration may lead to renal dysfunction.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "Effects of chronic oral D-galactose treatment on general health status in male Wistar rats",
pages = "115-118",
url = "https://hdl.handle.net/21.15107/rcub_dais_12378"
}

Martinović, J., Guševac Stojanović, I., Zarić, M., Todorović, A., Veljković, F., Pejić, S., Stojanović, Z., Mitrović, N., Grković, I.,& Drakulić, D.. (2021). Effects of chronic oral D-galactose treatment on general health status in male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 115-118.
https://hdl.handle.net/21.15107/rcub_dais_12378

Martinović J, Guševac Stojanović I, Zarić M, Todorović A, Veljković F, Pejić S, Stojanović Z, Mitrović N, Grković I, Drakulić D. Effects of chronic oral D-galactose treatment on general health status in male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:115-118.
https://hdl.handle.net/21.15107/rcub_dais_12378 .

Martinović, J., Guševac Stojanović, I., Zarić, M, Todorović, A., Veljković, F., Pejić, S., Stojanović, Zoran, Mitrović, N., Grković, I., Drakulić, Dunja, "Effects of chronic oral D-galactose treatment on general health status in male Wistar rats" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):115-118,
https://hdl.handle.net/21.15107/rcub_dais_12378 .