TY  - JOUR
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Rabanal, Maria Eugenia
AU  - Milošević, Milica
AU  - Bukumira, Marta
AU  - Tomić, Nina
AU  - Tomić, Miloš
AU  - Mančić, Lidija
AU  - Ignjatović, Nenad
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16211
AB  - Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals
SP  - 6
VL  - 15
IS  - 1
DO  - 10.3390/jfb15010006
UR  - https://hdl.handle.net/21.15107/rcub_dais_16211
ER  - 

@article{
author = "Dinić, Ivana and Vuković, Marina and Rabanal, Maria Eugenia and Milošević, Milica and Bukumira, Marta and Tomić, Nina and Tomić, Miloš and Mančić, Lidija and Ignjatović, Nenad",
year = "2024",
abstract = "Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals",
pages = "6",
volume = "15",
number = "1",
doi = "10.3390/jfb15010006",
url = "https://hdl.handle.net/21.15107/rcub_dais_16211"
}

Dinić, I., Vuković, M., Rabanal, M. E., Milošević, M., Bukumira, M., Tomić, N., Tomić, M., Mančić, L.,& Ignjatović, N.. (2024). Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials
Basel : MDPI AG., 15(1), 6.
https://doi.org/10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211

Dinić I, Vuković M, Rabanal ME, Milošević M, Bukumira M, Tomić N, Tomić M, Mančić L, Ignjatović N. Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials. 2024;15(1):6.
doi:10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211 .

Dinić, Ivana, Vuković, Marina, Rabanal, Maria Eugenia, Milošević, Milica, Bukumira, Marta, Tomić, Nina, Tomić, Miloš, Mančić, Lidija, Ignjatović, Nenad, "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals" in Journal of Functional Biomaterials, 15, no. 1 (2024):6,
https://doi.org/10.3390/jfb15010006 .,
https://hdl.handle.net/21.15107/rcub_dais_16211 .

TY  - CONF
AU  - Tomić, Nina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16245
AB  - Kontinuirano interesovanje za nanočestice različitih materijala i njihove kompozite stvorilo je brojna pitanja u vezi njihove potencijalne toksičnosti. Polifenol resveratrol i jedinjenja sa selenom su među agensima čijoj je upotrebi nanotehnologija omogućila nov pristup. U formi nanočestica, oni pokazuju poboljšanu biokompatibilnost i biološke efekte, kao što je antimikrobna aktivnost. Na osnovu podataka iz literature, nanočestice selena su najčešće manje toksične nego jedinjenja sa selenom. U našem istraživanju, najpre smo pripremili kompozitni materijal na bazi selena i resveratrola. Nakon toga, kompozit i njegove komponente bili su testirani pomoću MTT tetrazolijumske boje za utvrđivanje citotoksičnosti, komet testom na genotoksičnost i nitroplavo-tetrazolijumskim testom za procenu nivoa superoksidnih anjona. U svim eksperimentima je korišćena zdrava ćelijska linija fibroblasta čoveka (MRC-5). Rezultati su pokazali da, iako čestice resveratrola ispoljavaju citotoksičnost u koncentracijama preko 12 μg/ml, one nisu genotoksične i ne indukuju stvaranje superoksidnih anjona. Sa druge strane, nanočestice selena nisu bile citotoksične ispod 150 μg/ml, ali su njima tretirane ćelije pokazale znake oštećenja DNK i povećanje produkcije superoksidnih anjona, čak i na 18 μg/ml. Kompozit je pokazao drugačije efekte, indukujući višu citotoksičnost i oksidativni stres nego ekvivalentne koncentracije komponenti, ali istovremeno nije bio genotoksičan. Moguće je da su interakcije nanočestica selena sa nanočesticama resveratrola dovele do pojave jedinstvenih osobina kompozita, potvrđujući izuzetnu važnost testiranja nanokompozitnih materijala uporedo sa njihovim komponentama.
AB  - The continuous interest in engineered nanoparticles of various materials, and their composites, has raised numerous questions regarding their toxicological profiles. Polyphenol resveratrol and selenium compounds are among the agents to which nanotechnology has given all new approach. Their particulate forms possess improved bioavailability and biological effects, such as antibacterial activity. Furthermore, selenium nanoparticles usually have significantly lower toxicity compared to selenium compounds. In this study, we prepared composite material consisting of selenium and resveratrol nanoparticles. Then, composite material and its components were assessed by using tetrazolium MTT dye for determining cytotoxicity, comet assay for genotoxicity study, and nitroblue tetrazolium assay (NBT) assay to estimate levels of superoxide anions. The normal human fibroblast cells (MRC-5) were used in all the experiments. Results showed that, although the resveratrol component exhibited cytotoxicity at 12 μg/ml, it was not genotoxic, and did not induce superoxide anion production. Selenium nanoparticles, on the other hand, were not cytotoxic below 150 μg/ml, but treated cells showed signs of DNA damage and elevation in superoxide anion production even at 18 μg/ml. Composite material behaved differently, inducing higher cytotoxicity and oxidative stress than comparable concentrations of components, but also showed lack of genotoxicity. Possible interactions of selenium nanoparticles with resveratrol nanoparticles could have created unique composite properties, confirming the great importance of toxicity testing of nanocomposite materials along with their components.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
T1  - Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona
T1  - Cytotoxic and genotoxic potential of composite based on resveratrol and selenium nanoparticles, and its potential for superoxide anion induction
SP  - 193
EP  - 194
UR  - https://hdl.handle.net/21.15107/rcub_dais_16245
ER  - 

@conference{
author = "Tomić, Nina and Stevanović, Magdalena and Filipović, Nenad and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Kontinuirano interesovanje za nanočestice različitih materijala i njihove kompozite stvorilo je brojna pitanja u vezi njihove potencijalne toksičnosti. Polifenol resveratrol i jedinjenja sa selenom su među agensima čijoj je upotrebi nanotehnologija omogućila nov pristup. U formi nanočestica, oni pokazuju poboljšanu biokompatibilnost i biološke efekte, kao što je antimikrobna aktivnost. Na osnovu podataka iz literature, nanočestice selena su najčešće manje toksične nego jedinjenja sa selenom. U našem istraživanju, najpre smo pripremili kompozitni materijal na bazi selena i resveratrola. Nakon toga, kompozit i njegove komponente bili su testirani pomoću MTT tetrazolijumske boje za utvrđivanje citotoksičnosti, komet testom na genotoksičnost i nitroplavo-tetrazolijumskim testom za procenu nivoa superoksidnih anjona. U svim eksperimentima je korišćena zdrava ćelijska linija fibroblasta čoveka (MRC-5). Rezultati su pokazali da, iako čestice resveratrola ispoljavaju citotoksičnost u koncentracijama preko 12 μg/ml, one nisu genotoksične i ne indukuju stvaranje superoksidnih anjona. Sa druge strane, nanočestice selena nisu bile citotoksične ispod 150 μg/ml, ali su njima tretirane ćelije pokazale znake oštećenja DNK i povećanje produkcije superoksidnih anjona, čak i na 18 μg/ml. Kompozit je pokazao drugačije efekte, indukujući višu citotoksičnost i oksidativni stres nego ekvivalentne koncentracije komponenti, ali istovremeno nije bio genotoksičan. Moguće je da su interakcije nanočestica selena sa nanočesticama resveratrola dovele do pojave jedinstvenih osobina kompozita, potvrđujući izuzetnu važnost testiranja nanokompozitnih materijala uporedo sa njihovim komponentama., The continuous interest in engineered nanoparticles of various materials, and their composites, has raised numerous questions regarding their toxicological profiles. Polyphenol resveratrol and selenium compounds are among the agents to which nanotechnology has given all new approach. Their particulate forms possess improved bioavailability and biological effects, such as antibacterial activity. Furthermore, selenium nanoparticles usually have significantly lower toxicity compared to selenium compounds. In this study, we prepared composite material consisting of selenium and resveratrol nanoparticles. Then, composite material and its components were assessed by using tetrazolium MTT dye for determining cytotoxicity, comet assay for genotoxicity study, and nitroblue tetrazolium assay (NBT) assay to estimate levels of superoxide anions. The normal human fibroblast cells (MRC-5) were used in all the experiments. Results showed that, although the resveratrol component exhibited cytotoxicity at 12 μg/ml, it was not genotoxic, and did not induce superoxide anion production. Selenium nanoparticles, on the other hand, were not cytotoxic below 150 μg/ml, but treated cells showed signs of DNA damage and elevation in superoxide anion production even at 18 μg/ml. Composite material behaved differently, inducing higher cytotoxicity and oxidative stress than comparable concentrations of components, but also showed lack of genotoxicity. Possible interactions of selenium nanoparticles with resveratrol nanoparticles could have created unique composite properties, confirming the great importance of toxicity testing of nanocomposite materials along with their components.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book",
title = "Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona, Cytotoxic and genotoxic potential of composite based on resveratrol and selenium nanoparticles, and its potential for superoxide anion induction",
pages = "193-194",
url = "https://hdl.handle.net/21.15107/rcub_dais_16245"
}

Tomić, N., Stevanović, M., Filipović, N.,& Mitić Ćulafić, D.. (2023). Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
Beograd : Udruženje toksikologa Srbije., 193-194.
https://hdl.handle.net/21.15107/rcub_dais_16245

Tomić N, Stevanović M, Filipović N, Mitić Ćulafić D. Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book. 2023;:193-194.
https://hdl.handle.net/21.15107/rcub_dais_16245 .

Tomić, Nina, Stevanović, Magdalena, Filipović, Nenad, Mitić Ćulafić, Dragana, "Citotoksični i genotoksični potencijal kompozita na bazi nanočestica resveratrola i selena, i njegova sposobnost indukcije superoksidnih anjona" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book (2023):193-194,
https://hdl.handle.net/21.15107/rcub_dais_16245 .

TY  - CONF
AU  - Ganić, Tea
AU  - Cvetković, Stefana
AU  - Vuletić, Stefana
AU  - Stević, Tatjana
AU  - Stevanović, Magdalena
AU  - Tomić, Nina
AU  - Nikolić, Biljana
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16246
AB  - Cimet je jedna od najpoznatijih aromatičnih biljaka, koje je najčešće korišćena kao začin. Prepoznat je kao potencijalni terapeutik u narodnoj medicini, zbog čega se dugo koristi za lečenje različitih infekcija. Etarska ulja su sekundarni metaboliti koje sintetišu aromatične biljke. Za etarsko ulje cimeta (CEO) su definisane različite biološke aktivnosti, međutim fizičkohemijske karakteristike ulja su slaba rastvorljivost u vodi i laka isparljivost, što ograničava njihovu primenu. Iz tog razloga, formulacija emulzija etarskih ulja predstavlja dobro rešenje za prevazilaženje problema njihove primene. Iako se CEO smatra bezbednim za upotrebu, rezultati istraživanja variraju, ukazujući na neophodnost daljih toksikoloških istraživanja. Cilj ovog rada je bila komparativna analiza biološke aktivnosti i in vitro toksičnosti CEO i njegove emulzije (EM). Antioksidativni potencijal je ispitivan DPPH testom. MTT test je primenjen u cilju procene citotoksičnog efekta CEO i EM na fibroblastima pluća fetusa (MRC–5). Na istoj ćelijskoj liniji, ispitivan je nivo oštećenja molekula DNK, primenom alkalnog komet testa. Na osnovu rezltata DPPH testa, IC50 vrednost za CEO je bila 320 μg/mL, dok je za EM bila 54 μg/mL. Na ćelijskoj liniji MRC–5, IC50 vrednosti dobijene MTT testom su iznosile 0,13 mg/mL za CEO i 0,025 mg/mL za EM. CEO i EM nisu pokazali genotoksični potencijal. Uzimajući sve prethodno navedeno u obzir, CEO i EM, kao jaki antioksidanti koji pokazuju odsustvo genotoksičnosti i slabu citotoksičnost.
AB  - Cinnamon is among most popular aromatic plants, generally used as a spice. Over the centuries it was recognized as a potential therapeutic for different kind of infections. Essential oils are aromatic plants’ secondary metabolites. Cinnamon essential oil (CEO) possess many favorable biological activities, but its physicochemical properties, as an oil, are poor solubility in water and high volatility, which limits its application. For that reason, preparation of essential oils’ emulsions seems to be solution to overcome applicability problems. Even though CEO is one of the oils recognized as safe to be used, the results from different studies vary, indicating necessity to further study its toxicological properties. The aim of this study was to comparatively analyze CEO and its emulsion (EM) biological activity and in vitro toxicity. Antioxidative potential was investigated using DPPH assay. The MTT assay was applied for the estimation of cytotoxic effects of CEO and EM on fetal fibroblasts (MRC-5) cell line. On the same cell line, level of DNA damage was evaluated, using alkaline comet assay. According to the DPPH assay results, IC50 value for CEO was 320 μg/mL, for EM was 54 μg/mL. On MRC-5 cell line, IC50 values were 0.13 mg/mL for CEO and 0.025 mg/mL for EM. Both CEO and EM didn’t exhibit potential to induce DNA damage. Taking all above mention into account, as strong antioxidants without genotoxicity and with weak cytotoxicity, CEO and EM could be considered as good candidates for further investigation of biological activities and potential for human use.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
T1  - Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije
T1  - Evaluation of antioxidative, cytotoxic and genotoxic effect of cinnamon essential oil and its emulsion
SP  - 152
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_dais_16246
ER  - 

@conference{
author = "Ganić, Tea and Cvetković, Stefana and Vuletić, Stefana and Stević, Tatjana and Stevanović, Magdalena and Tomić, Nina and Nikolić, Biljana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Cimet je jedna od najpoznatijih aromatičnih biljaka, koje je najčešće korišćena kao začin. Prepoznat je kao potencijalni terapeutik u narodnoj medicini, zbog čega se dugo koristi za lečenje različitih infekcija. Etarska ulja su sekundarni metaboliti koje sintetišu aromatične biljke. Za etarsko ulje cimeta (CEO) su definisane različite biološke aktivnosti, međutim fizičkohemijske karakteristike ulja su slaba rastvorljivost u vodi i laka isparljivost, što ograničava njihovu primenu. Iz tog razloga, formulacija emulzija etarskih ulja predstavlja dobro rešenje za prevazilaženje problema njihove primene. Iako se CEO smatra bezbednim za upotrebu, rezultati istraživanja variraju, ukazujući na neophodnost daljih toksikoloških istraživanja. Cilj ovog rada je bila komparativna analiza biološke aktivnosti i in vitro toksičnosti CEO i njegove emulzije (EM). Antioksidativni potencijal je ispitivan DPPH testom. MTT test je primenjen u cilju procene citotoksičnog efekta CEO i EM na fibroblastima pluća fetusa (MRC–5). Na istoj ćelijskoj liniji, ispitivan je nivo oštećenja molekula DNK, primenom alkalnog komet testa. Na osnovu rezltata DPPH testa, IC50 vrednost za CEO je bila 320 μg/mL, dok je za EM bila 54 μg/mL. Na ćelijskoj liniji MRC–5, IC50 vrednosti dobijene MTT testom su iznosile 0,13 mg/mL za CEO i 0,025 mg/mL za EM. CEO i EM nisu pokazali genotoksični potencijal. Uzimajući sve prethodno navedeno u obzir, CEO i EM, kao jaki antioksidanti koji pokazuju odsustvo genotoksičnosti i slabu citotoksičnost., Cinnamon is among most popular aromatic plants, generally used as a spice. Over the centuries it was recognized as a potential therapeutic for different kind of infections. Essential oils are aromatic plants’ secondary metabolites. Cinnamon essential oil (CEO) possess many favorable biological activities, but its physicochemical properties, as an oil, are poor solubility in water and high volatility, which limits its application. For that reason, preparation of essential oils’ emulsions seems to be solution to overcome applicability problems. Even though CEO is one of the oils recognized as safe to be used, the results from different studies vary, indicating necessity to further study its toxicological properties. The aim of this study was to comparatively analyze CEO and its emulsion (EM) biological activity and in vitro toxicity. Antioxidative potential was investigated using DPPH assay. The MTT assay was applied for the estimation of cytotoxic effects of CEO and EM on fetal fibroblasts (MRC-5) cell line. On the same cell line, level of DNA damage was evaluated, using alkaline comet assay. According to the DPPH assay results, IC50 value for CEO was 320 μg/mL, for EM was 54 μg/mL. On MRC-5 cell line, IC50 values were 0.13 mg/mL for CEO and 0.025 mg/mL for EM. Both CEO and EM didn’t exhibit potential to induce DNA damage. Taking all above mention into account, as strong antioxidants without genotoxicity and with weak cytotoxicity, CEO and EM could be considered as good candidates for further investigation of biological activities and potential for human use.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book",
title = "Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije, Evaluation of antioxidative, cytotoxic and genotoxic effect of cinnamon essential oil and its emulsion",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_dais_16246"
}

Ganić, T., Cvetković, S., Vuletić, S., Stević, T., Stevanović, M., Tomić, N., Nikolić, B.,& Mitić Ćulafić, D.. (2023). Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
Beograd : Udruženje toksikologa Srbije., 152-153.
https://hdl.handle.net/21.15107/rcub_dais_16246

Ganić T, Cvetković S, Vuletić S, Stević T, Stevanović M, Tomić N, Nikolić B, Mitić Ćulafić D. Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book. 2023;:152-153.
https://hdl.handle.net/21.15107/rcub_dais_16246 .

Ganić, Tea, Cvetković, Stefana, Vuletić, Stefana, Stević, Tatjana, Stevanović, Magdalena, Tomić, Nina, Nikolić, Biljana, Mitić Ćulafić, Dragana, "Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book (2023):152-153,
https://hdl.handle.net/21.15107/rcub_dais_16246 .

TY  - CONF
AU  - Vuletić, Stefana
AU  - Cvetković, Stefana
AU  - Tomić, Nina
AU  - Ganić, Tea
AU  - Tomić, Sergej
AU  - Bekić, Marina
AU  - Nikolić, Biljana
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16247
AB  - Hepatocelularni i kolorektalni karcinom se sve ćešće javlja i ima visoku stopu smrtnosti. Postojanje aktivnih mehanizama hemorezistencije otežava korisnost hemoterapije. Upravo je pojava hemorezistencije podstakla opsežna istraživanja za razvoj novih terapeutskih agenasa i strategija za lečenje ovih vrsta karcinoma. Prirodni proizvodi biljnog porekla (sekundarni metaboliti) su bogat izvor potencijalnih lekova. Zbog toga smo u istraživanju usmerili pažnju na etil-acetatni ekstrakt biljke Frangula alnus (FA) i njegovu dominantnu komponentu emodin (E) i ispitali njihovo antikancerski potencijal na ćelijama hepatocelularnog (HepG2) i kolorektalnog (HCT116) karcinoma i citotoksičnost na normalnim MRC -5 ćelijama. Pokazan je snažan antioksidativni potencijal ekstrakta (DPPH i TBA test). Citotoksičnost ispitana MTT testom pokazala je jaku toksičnost obe supstance na ćelije HepG2 i HCT116, ali bez uticaja na MRC-5 ćelije. Dalje je analiza ćelijskog ciklusa pokazala da su i FA i E izazvali zaustavljanje u G1 fazi i blago nakupljanje ćelija u G2/M fazi. Pored toga, obe supstance su uvele ćelije u apoptozu i nekrozu i uticale su na mitohondrijalni membranski potencijal. Dalje, izazvali su značajan genotoksični efekat u kometnom testu na svim ćelijskim linijama. Može se zaključiti da su FA i E dobri kandidati za nove antikancerogene agense prirodnog porekla. Međutim, neophodna su dodatna istraživanja, posebno kada je u pitanju citotoksična aktivnost i bezbednost primene ovih supstanci.
AB  - Hepatocellular and colorectal carcinoma is swiftly increasing alongside a high mortality rate. The existence of active mechanisms of chemoresistance hampers the usefulness of chemotherapy. The emergence of chemoresistance encouraged extensive research to develop new therapeutic agents and strategies for the treatment of these types of cancer. Natural products of plant origin (secondary metabolites) have been the most successful source of potential drug leads. Therefore, we turned our attention to the Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) and explored them for their anticancer potential on hepatocellular (HepG2) and colorectal (HCT116) carcinoma cells, and cytotoxicity on normal MRC-5 cells. Strong antioxidant potential of the extract was demonstrated (DPPH and TBA test). Cytotoxicity investigated by the MTT assay showed strong cell toxicity of both substances on HepG2 and HCT116 cells, but without affecting MRC-5 cells. Next, the analysis of cell cycles exhibited that both FA and E induced arrest in the G1 phase and slight accumulation of cells in the G2/M phase. In addition, both substances introduced cells into apoptosis and necrosis and modulated mitochondrial membrane potential. Further on, they caused significant genotoxic effect in comet assay applied in all cell lines. It can be concluded that FA and E are good candidates for new anticancer agents of natural origin. However, additional studies are necessary, especially when it comes to the cytotoxic activity and safety of the application of these substances.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023
T1  - Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi
T1  - Frangula alnus extract and emodin as potential anticancer agents
SP  - 148
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_dais_16247
ER  - 

@conference{
author = "Vuletić, Stefana and Cvetković, Stefana and Tomić, Nina and Ganić, Tea and Tomić, Sergej and Bekić, Marina and Nikolić, Biljana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Hepatocelularni i kolorektalni karcinom se sve ćešće javlja i ima visoku stopu smrtnosti. Postojanje aktivnih mehanizama hemorezistencije otežava korisnost hemoterapije. Upravo je pojava hemorezistencije podstakla opsežna istraživanja za razvoj novih terapeutskih agenasa i strategija za lečenje ovih vrsta karcinoma. Prirodni proizvodi biljnog porekla (sekundarni metaboliti) su bogat izvor potencijalnih lekova. Zbog toga smo u istraživanju usmerili pažnju na etil-acetatni ekstrakt biljke Frangula alnus (FA) i njegovu dominantnu komponentu emodin (E) i ispitali njihovo antikancerski potencijal na ćelijama hepatocelularnog (HepG2) i kolorektalnog (HCT116) karcinoma i citotoksičnost na normalnim MRC -5 ćelijama. Pokazan je snažan antioksidativni potencijal ekstrakta (DPPH i TBA test). Citotoksičnost ispitana MTT testom pokazala je jaku toksičnost obe supstance na ćelije HepG2 i HCT116, ali bez uticaja na MRC-5 ćelije. Dalje je analiza ćelijskog ciklusa pokazala da su i FA i E izazvali zaustavljanje u G1 fazi i blago nakupljanje ćelija u G2/M fazi. Pored toga, obe supstance su uvele ćelije u apoptozu i nekrozu i uticale su na mitohondrijalni membranski potencijal. Dalje, izazvali su značajan genotoksični efekat u kometnom testu na svim ćelijskim linijama. Može se zaključiti da su FA i E dobri kandidati za nove antikancerogene agense prirodnog porekla. Međutim, neophodna su dodatna istraživanja, posebno kada je u pitanju citotoksična aktivnost i bezbednost primene ovih supstanci., Hepatocellular and colorectal carcinoma is swiftly increasing alongside a high mortality rate. The existence of active mechanisms of chemoresistance hampers the usefulness of chemotherapy. The emergence of chemoresistance encouraged extensive research to develop new therapeutic agents and strategies for the treatment of these types of cancer. Natural products of plant origin (secondary metabolites) have been the most successful source of potential drug leads. Therefore, we turned our attention to the Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) and explored them for their anticancer potential on hepatocellular (HepG2) and colorectal (HCT116) carcinoma cells, and cytotoxicity on normal MRC-5 cells. Strong antioxidant potential of the extract was demonstrated (DPPH and TBA test). Cytotoxicity investigated by the MTT assay showed strong cell toxicity of both substances on HepG2 and HCT116 cells, but without affecting MRC-5 cells. Next, the analysis of cell cycles exhibited that both FA and E induced arrest in the G1 phase and slight accumulation of cells in the G2/M phase. In addition, both substances introduced cells into apoptosis and necrosis and modulated mitochondrial membrane potential. Further on, they caused significant genotoxic effect in comet assay applied in all cell lines. It can be concluded that FA and E are good candidates for new anticancer agents of natural origin. However, additional studies are necessary, especially when it comes to the cytotoxic activity and safety of the application of these substances.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023",
title = "Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi, Frangula alnus extract and emodin as potential anticancer agents",
pages = "148-149",
url = "https://hdl.handle.net/21.15107/rcub_dais_16247"
}

Vuletić, S., Cvetković, S., Tomić, N., Ganić, T., Tomić, S., Bekić, M., Nikolić, B.,& Mitić Ćulafić, D.. (2023). Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023
Beograd : Udruženje toksikologa Srbije., 148-149.
https://hdl.handle.net/21.15107/rcub_dais_16247

Vuletić S, Cvetković S, Tomić N, Ganić T, Tomić S, Bekić M, Nikolić B, Mitić Ćulafić D. Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023. 2023;:148-149.
https://hdl.handle.net/21.15107/rcub_dais_16247 .

Vuletić, Stefana, Cvetković, Stefana, Tomić, Nina, Ganić, Tea, Tomić, Sergej, Bekić, Marina, Nikolić, Biljana, Mitić Ćulafić, Dragana, "Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023 (2023):148-149,
https://hdl.handle.net/21.15107/rcub_dais_16247 .

TY  - JOUR
AU  - Tomić, Nina
AU  - Matić, Tamara
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Boccacccini, Aldo R.
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15195
AB  - Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.
PB  - SAGE Publications Ltd.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties
SP  - 122
EP  - 133
VL  - 38
IS  - 1
DO  - 10.1177/08853282231183109
UR  - https://hdl.handle.net/21.15107/rcub_dais_15195
ER  - 

@article{
author = "Tomić, Nina and Matić, Tamara and Filipović, Nenad and Mitić Ćulafić, Dragana and Boccacccini, Aldo R. and Stevanović, Magdalena",
year = "2023",
abstract = "Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.",
publisher = "SAGE Publications Ltd.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties",
pages = "122-133",
volume = "38",
number = "1",
doi = "10.1177/08853282231183109",
url = "https://hdl.handle.net/21.15107/rcub_dais_15195"
}

Tomić, N., Matić, T., Filipović, N., Mitić Ćulafić, D., Boccacccini, A. R.,& Stevanović, M.. (2023). Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications
SAGE Publications Ltd.., 38(1), 122-133.
https://doi.org/10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_15195

Tomić N, Matić T, Filipović N, Mitić Ćulafić D, Boccacccini AR, Stevanović M. Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications. 2023;38(1):122-133.
doi:10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_15195 .

Tomić, Nina, Matić, Tamara, Filipović, Nenad, Mitić Ćulafić, Dragana, Boccacccini, Aldo R., Stevanović, Magdalena, "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties" in Journal of Biomaterials Applications, 38, no. 1 (2023):122-133,
https://doi.org/10.1177/08853282231183109 .,
https://hdl.handle.net/21.15107/rcub_dais_15195 .

TY  - CONF
AU  - Kuzmanović, Maja
AU  - Filipović, Nenad
AU  - Tomić, Nina
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15225
AB  - As the most abundant protein in the human body, collagen has been drawing the attention of many researchers from the biomedical field for a long time. It can be found as a constituent in versatile commercially available products. In the hydrogel form, it is used often in designing novel platforms for drug delivery or cell growth. On the other side, Selenium nanoparticles (SeNPs) are a relatively new selenium form that has proven records in enhanced antimicrobial, anticancer, antiviral, antioxidative activity, and reduced toxicity compared to other selenium forms. Therefore, in this work, we have examined the effects of different stabilizing agents, used in the preparation of selenium nanoparticles, on the formation, structure, and biocompatibility of composite hydrogel Collagen/SeNPs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), optical microscopy, and cell viability (MTT assay) were used for the characterizations of obtained hydrogels. SeNPs were synthesized by the chemical reduction in the form of colloidal solutions using (i) bovine serum albumin, (ii) chitosan, and(iii) glucose as stabilizing agents.
PB  - Belgrade : Serbian Ceramic Society
C3  - The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles
T1  - The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles
SP  - 86
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_dais_15225
ER  - 

@conference{
author = "Kuzmanović, Maja and Filipović, Nenad and Tomić, Nina and Stevanović, Magdalena",
year = "2023",
abstract = "As the most abundant protein in the human body, collagen has been drawing the attention of many researchers from the biomedical field for a long time. It can be found as a constituent in versatile commercially available products. In the hydrogel form, it is used often in designing novel platforms for drug delivery or cell growth. On the other side, Selenium nanoparticles (SeNPs) are a relatively new selenium form that has proven records in enhanced antimicrobial, anticancer, antiviral, antioxidative activity, and reduced toxicity compared to other selenium forms. Therefore, in this work, we have examined the effects of different stabilizing agents, used in the preparation of selenium nanoparticles, on the formation, structure, and biocompatibility of composite hydrogel Collagen/SeNPs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), optical microscopy, and cell viability (MTT assay) were used for the characterizations of obtained hydrogels. SeNPs were synthesized by the chemical reduction in the form of colloidal solutions using (i) bovine serum albumin, (ii) chitosan, and(iii) glucose as stabilizing agents.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles",
title = "The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles",
pages = "86-86",
url = "https://hdl.handle.net/21.15107/rcub_dais_15225"
}

Kuzmanović, M., Filipović, N., Tomić, N.,& Stevanović, M.. (2023). The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles. in The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles
Belgrade : Serbian Ceramic Society., 86-86.
https://hdl.handle.net/21.15107/rcub_dais_15225

Kuzmanović M, Filipović N, Tomić N, Stevanović M. The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles. in The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles. 2023;:86-86.
https://hdl.handle.net/21.15107/rcub_dais_15225 .

Kuzmanović, Maja, Filipović, Nenad, Tomić, Nina, Stevanović, Magdalena, "The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles" in The effect of stabilizing agents on physicochemical properties and cell viability of composite hydrogel Collagen/Selenium nanoparticles (2023):86-86,
https://hdl.handle.net/21.15107/rcub_dais_15225 .

TY  - CONF
AU  - Filipović, Nenad
AU  - Tomić, Nina
AU  - Kuzmanović, Maja
AU  - Stojanović, Zoran
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15762
AB  - Nanotechnology, as a most promising approach in material science, has resulted in numerous enhancements and breakthroughs in diverse scientific fields. One of the examples is selenium nanoparticles (SeNPs) which emerged as a new form with some improved properties compared to other Se forms. Among these properties, anticancer, antimicrobial, antioxidative, and reduced toxicity are the most interesting from the aspect of biomedical applications. Due to simplicity, short duration, scalability, and reproducibility, chemical reduction is a synthesis technique very often applied in SeNPs production. The choice of reducing agents, their molar ratio with a precursor, and the choice of stabilizing agents are recognized as determining parameters for the application efficiency of designed SeNPs.
This presentation includes an overview of the results of SeNPs obtained by the reduction of sodium selenite and stabilized with different agents. Furthermore, the effects of synthesis parameters on the properties of obtained particles (size, morphology, crystallinity, stability, surface chemistry) and biological activities such as antimicrobial, antioxidative, and cytotoxicity will be elaborate. In addition, some potential applications of SeNPs will be discussed, with particular reference to the results of in vivo experiments.
PB  - Ljubljana : Inštitut za kovinske materiale in tehnologije
C3  - 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
T1  - Selenium nanoparticles: Effects of particle properties on biological activity
SP  - 32
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_dais_15762
ER  - 

@conference{
author = "Filipović, Nenad and Tomić, Nina and Kuzmanović, Maja and Stojanović, Zoran and Stevanović, Magdalena",
year = "2023",
abstract = "Nanotechnology, as a most promising approach in material science, has resulted in numerous enhancements and breakthroughs in diverse scientific fields. One of the examples is selenium nanoparticles (SeNPs) which emerged as a new form with some improved properties compared to other Se forms. Among these properties, anticancer, antimicrobial, antioxidative, and reduced toxicity are the most interesting from the aspect of biomedical applications. Due to simplicity, short duration, scalability, and reproducibility, chemical reduction is a synthesis technique very often applied in SeNPs production. The choice of reducing agents, their molar ratio with a precursor, and the choice of stabilizing agents are recognized as determining parameters for the application efficiency of designed SeNPs.
This presentation includes an overview of the results of SeNPs obtained by the reduction of sodium selenite and stabilized with different agents. Furthermore, the effects of synthesis parameters on the properties of obtained particles (size, morphology, crystallinity, stability, surface chemistry) and biological activities such as antimicrobial, antioxidative, and cytotoxicity will be elaborate. In addition, some potential applications of SeNPs will be discussed, with particular reference to the results of in vivo experiments.",
publisher = "Ljubljana : Inštitut za kovinske materiale in tehnologije",
journal = "28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology",
title = "Selenium nanoparticles: Effects of particle properties on biological activity",
pages = "32-32",
url = "https://hdl.handle.net/21.15107/rcub_dais_15762"
}

Filipović, N., Tomić, N., Kuzmanović, M., Stojanović, Z.,& Stevanović, M.. (2023). Selenium nanoparticles: Effects of particle properties on biological activity. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
Ljubljana : Inštitut za kovinske materiale in tehnologije., 32-32.
https://hdl.handle.net/21.15107/rcub_dais_15762

Filipović N, Tomić N, Kuzmanović M, Stojanović Z, Stevanović M. Selenium nanoparticles: Effects of particle properties on biological activity. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology. 2023;:32-32.
https://hdl.handle.net/21.15107/rcub_dais_15762 .

Filipović, Nenad, Tomić, Nina, Kuzmanović, Maja, Stojanović, Zoran, Stevanović, Magdalena, "Selenium nanoparticles: Effects of particle properties on biological activity" in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology (2023):32-32,
https://hdl.handle.net/21.15107/rcub_dais_15762 .

TY  - CONF
AU  - Tomić, Nina
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Bukumira, Marta
AU  - Rabasović, Mihailo
AU  - Mančić, Lidija
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15155
AB  - There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles (UCNPs) with controlled crystal phase, morphology and intense luminescence properties suitable for biomedical use. A conventional approach for synthesis of such particles comprises decomposition of organometallic compounds in an oxygen-free environment, followed either with a ligand exchange, or biocompatible layer coating. Biocompatible NaY0.65Gd0.15F4:Yb,Er nanoparticles used in this study were synthesized through chitosan assisted one-pot hydrothermal synthesis and were characterized by X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and photoluminescence measurement (PL). Due to the presence of the amino groups at their surface, excellent biocompatibility and notably low cytotoxicity against MRC-5 cells (line of normal human fibroblasts) and A549 cells (human lung cancer cells) were detected using MTT assay. Furthermore, upon 980 nm laser irradiation, particles were successfully used in vitro for labeling of both, MRC-5 and A549 cells.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
T1  - One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_dais_15155
ER  - 

@conference{
author = "Tomić, Nina and Dinić, Ivana and Vuković, Marina and Bukumira, Marta and Rabasović, Mihailo and Mančić, Lidija",
year = "2023",
abstract = "There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles (UCNPs) with controlled crystal phase, morphology and intense luminescence properties suitable for biomedical use. A conventional approach for synthesis of such particles comprises decomposition of organometallic compounds in an oxygen-free environment, followed either with a ligand exchange, or biocompatible layer coating. Biocompatible NaY0.65Gd0.15F4:Yb,Er nanoparticles used in this study were synthesized through chitosan assisted one-pot hydrothermal synthesis and were characterized by X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and photoluminescence measurement (PL). Due to the presence of the amino groups at their surface, excellent biocompatibility and notably low cytotoxicity against MRC-5 cells (line of normal human fibroblasts) and A549 cells (human lung cancer cells) were detected using MTT assay. Furthermore, upon 980 nm laser irradiation, particles were successfully used in vitro for labeling of both, MRC-5 and A549 cells.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023",
title = "One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_dais_15155"
}

Tomić, N., Dinić, I., Vuković, M., Bukumira, M., Rabasović, M.,& Mančić, L.. (2023). One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
Belgrade : Serbian Ceramic Society., 70-70.
https://hdl.handle.net/21.15107/rcub_dais_15155

Tomić N, Dinić I, Vuković M, Bukumira M, Rabasović M, Mančić L. One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023. 2023;:70-70.
https://hdl.handle.net/21.15107/rcub_dais_15155 .

Tomić, Nina, Dinić, Ivana, Vuković, Marina, Bukumira, Marta, Rabasović, Mihailo, Mančić, Lidija, "One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 (2023):70-70,
https://hdl.handle.net/21.15107/rcub_dais_15155 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15749
AB  - There is a growing need for multifunctional components in material design, especially in tissue engineering. Among many natural compounds, polyphenols are gaining interest as biologically active additives of novel materials. Stilbenoid polyphenol resveratrol is one of the most known, mainly because of its antioxidative, antibacterial, anticancer, neuroprotective and other beneficial effects. To allow most efficient use of resveratrol, several nanoformulations have shown promise, but most of them also involve other, toxic or expensive compounds, beside resveratrol. During our research, we synthetized particles of pure resveratrol, in an elongated, nanobelt-like form. The shape of the particles, as well as absence of any polymer or other carriers makes these particles easy to handle for further implementation. For preliminary assessment of the functionality of these particles, several assays were employed. DPPH, TBA and FRAP assay proved antioxidative potential of obtained particles by several mechanisms in the concentration range from 1 μg/mL to 10 μg/mL. Artemia salina in vivo bioassay was used to show high bioactivity and also biocompatibility of the particle suspension. All of the results indicated that there is a high potential for use of these resv
PB  - Ljubljana : Inštitut za kovinske materiale in tehnologije
C3  - 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
T1  - Antioxidative Resveratrol Particles as a Bioactive Component for Material Design
SP  - 87
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_dais_15749
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić Ćulafić, Dragana and Stevanović, Magdalena",
year = "2023",
abstract = "There is a growing need for multifunctional components in material design, especially in tissue engineering. Among many natural compounds, polyphenols are gaining interest as biologically active additives of novel materials. Stilbenoid polyphenol resveratrol is one of the most known, mainly because of its antioxidative, antibacterial, anticancer, neuroprotective and other beneficial effects. To allow most efficient use of resveratrol, several nanoformulations have shown promise, but most of them also involve other, toxic or expensive compounds, beside resveratrol. During our research, we synthetized particles of pure resveratrol, in an elongated, nanobelt-like form. The shape of the particles, as well as absence of any polymer or other carriers makes these particles easy to handle for further implementation. For preliminary assessment of the functionality of these particles, several assays were employed. DPPH, TBA and FRAP assay proved antioxidative potential of obtained particles by several mechanisms in the concentration range from 1 μg/mL to 10 μg/mL. Artemia salina in vivo bioassay was used to show high bioactivity and also biocompatibility of the particle suspension. All of the results indicated that there is a high potential for use of these resv",
publisher = "Ljubljana : Inštitut za kovinske materiale in tehnologije",
journal = "28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology",
title = "Antioxidative Resveratrol Particles as a Bioactive Component for Material Design",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_dais_15749"
}

Tomić, N., Filipović, N., Mitić Ćulafić, D.,& Stevanović, M.. (2023). Antioxidative Resveratrol Particles as a Bioactive Component for Material Design. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology
Ljubljana : Inštitut za kovinske materiale in tehnologije., 87-87.
https://hdl.handle.net/21.15107/rcub_dais_15749

Tomić N, Filipović N, Mitić Ćulafić D, Stevanović M. Antioxidative Resveratrol Particles as a Bioactive Component for Material Design. in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_dais_15749 .

Tomić, Nina, Filipović, Nenad, Mitić Ćulafić, Dragana, Stevanović, Magdalena, "Antioxidative Resveratrol Particles as a Bioactive Component for Material Design" in 28. Mednarodna konferenca o materialih in tehnologijah = 28th International Conference on Materials and Technology (2023):87-87,
https://hdl.handle.net/21.15107/rcub_dais_15749 .

TY  - JOUR
AU  - Maksimovic, Milica
AU  - Jovanović, Marina
AU  - Nikolić, Biljana
AU  - Tomić, Nina
AU  - Tenji, Dina
AU  - Stević, Tatjana
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14551
AB  - Persicaria amphibia (subfam. Polygonoideae), an aquatic macrophyte rich in dietary polyphenolics, is used as a traditional remedy and culinary herb. Nevertheless, P. amphibia from the Balkan region has been insufficiently studied and underutilized. Here, the cytotoxicity and antimicrobial properties of the previously chemically characterised ethanol extract of P. amphibia aerial parts were tested. The following methods were carried out: the MTT assay, qRT-PCR, microdilution assay, Chromobacterium violaceum screening assay (monitoring of quorum sensing, QS) and the agar plating method (antifungal activity). The study was conducted to determine the cytotoxic effects of P. amphibia against lung cancer cells (A549) and its combination with cytostatic doxorubicin (Dox). A dose-dependent decrease in cell viability (up to 82% reduction) and additive interactions of the tested agents were noted. Both alone and combined with Dox, P. amphibia reduced the expression of Nrf2 (p < 0.05). In terms of antimicrobial effects, P. amphibia exhibited an antipathogenic effect since it disrupted QS communication, which was evident through the inhibition of violacein production of C. violaceum CV025. The antifungal screening revealed that P. amphibia induced significant growth inhibition of Aspergillus spp. (28.23%). Based on the obtained results, further examination of the potential use of P. amphibia in modern phytotherapy and diet-derived cancer chemoprevention is encouraged.
PB  - Belgrade : 	Institute of Botany and Botanical Garden Jevremovac (University of Belgrade - Faculty of Biology)
T2  - Botanica Serbica
T1  - Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties
SP  - 1
EP  - 8
VL  - 47
IS  - 1
DO  - 10.2298/BOTSERB2301001M
UR  - https://hdl.handle.net/21.15107/rcub_dais_14551
ER  - 

@article{
author = "Maksimovic, Milica and Jovanović, Marina and Nikolić, Biljana and Tomić, Nina and Tenji, Dina and Stević, Tatjana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Persicaria amphibia (subfam. Polygonoideae), an aquatic macrophyte rich in dietary polyphenolics, is used as a traditional remedy and culinary herb. Nevertheless, P. amphibia from the Balkan region has been insufficiently studied and underutilized. Here, the cytotoxicity and antimicrobial properties of the previously chemically characterised ethanol extract of P. amphibia aerial parts were tested. The following methods were carried out: the MTT assay, qRT-PCR, microdilution assay, Chromobacterium violaceum screening assay (monitoring of quorum sensing, QS) and the agar plating method (antifungal activity). The study was conducted to determine the cytotoxic effects of P. amphibia against lung cancer cells (A549) and its combination with cytostatic doxorubicin (Dox). A dose-dependent decrease in cell viability (up to 82% reduction) and additive interactions of the tested agents were noted. Both alone and combined with Dox, P. amphibia reduced the expression of Nrf2 (p < 0.05). In terms of antimicrobial effects, P. amphibia exhibited an antipathogenic effect since it disrupted QS communication, which was evident through the inhibition of violacein production of C. violaceum CV025. The antifungal screening revealed that P. amphibia induced significant growth inhibition of Aspergillus spp. (28.23%). Based on the obtained results, further examination of the potential use of P. amphibia in modern phytotherapy and diet-derived cancer chemoprevention is encouraged.",
publisher = "Belgrade : 	Institute of Botany and Botanical Garden Jevremovac (University of Belgrade - Faculty of Biology)",
journal = "Botanica Serbica",
title = "Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties",
pages = "1-8",
volume = "47",
number = "1",
doi = "10.2298/BOTSERB2301001M",
url = "https://hdl.handle.net/21.15107/rcub_dais_14551"
}

Maksimovic, M., Jovanović, M., Nikolić, B., Tomić, N., Tenji, D., Stević, T.,& Mitić Ćulafić, D.. (2023). Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties. in Botanica Serbica
Belgrade : 	Institute of Botany and Botanical Garden Jevremovac (University of Belgrade - Faculty of Biology)., 47(1), 1-8.
https://doi.org/10.2298/BOTSERB2301001M
https://hdl.handle.net/21.15107/rcub_dais_14551

Maksimovic M, Jovanović M, Nikolić B, Tomić N, Tenji D, Stević T, Mitić Ćulafić D. Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties. in Botanica Serbica. 2023;47(1):1-8.
doi:10.2298/BOTSERB2301001M
https://hdl.handle.net/21.15107/rcub_dais_14551 .

Maksimovic, Milica, Jovanović, Marina, Nikolić, Biljana, Tomić, Nina, Tenji, Dina, Stević, Tatjana, Mitić Ćulafić, Dragana, "Persicaria amphibia, an old traditional remedy and wild edible herb: In vitro evaluation of cytotoxicity and antimicrobial properties" in Botanica Serbica, 47, no. 1 (2023):1-8,
https://doi.org/10.2298/BOTSERB2301001M .,
https://hdl.handle.net/21.15107/rcub_dais_14551 .

TY  - JOUR
AU  - Tomić, Nina
AU  - Matić, Tamara
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Boccacccini, Aldo R.
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14705
AB  - Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.
PB  - SAGE Publications Ltd.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties
SP  - 122
EP  - 133
VL  - 38
IS  - 1
DO  - 10.1177/08853282231183109
UR  - https://hdl.handle.net/21.15107/rcub_dais_14705
ER  - 

@article{
author = "Tomić, Nina and Matić, Tamara and Filipović, Nenad and Mitić Ćulafić, Dragana and Boccacccini, Aldo R. and Stevanović, Magdalena",
year = "2023",
abstract = "Recently, many studies have shown various beneficial effects of polyphenol resveratrol (Res) on human health. The most important of these effects include cardioprotective, neuroprotective, anti-cancer, anti-inflammatory, osteoinductive, and anti-microbial effects. Resveratrol has cis and trans isoforms, with the trans isoform being more stable and biologically active. Despite the results of in vitro experiments, resveratrol has limited potential for application in vivo due to its poor water solubility, sensitivity to oxygen, light, and heat, rapid metabolism, and therefore low bioavailability. The possible solution to overcome these limitations could be the synthesis of resveratrol in nanoparticle form. Accordingly, in this study, we have developed a simple, green solvent/non-solvent physicochemical method to synthesize stable, uniform, carrier-free resveratrol nanobelt-like particles (ResNPs) for applications in tissue engineering. UV–visible spectroscopy (UV-Vis) was used to identify the trans isoform of ResNPs which remained stable for at least 63 days. The additional qualitative analysis was performed by Fourier transform infrared spectroscopy (FTIR), while X-ray diffraction (XRD) determined the monoclinic structure of resveratrol with a significant difference in the intensity of diffraction peaks between commercial and nano-belt form. The morphology of ResNPs was evaluated by optical microscopy and field-emission scanning electron microscope (FE-SEM) that revealed a uniform nanobelt-like structure with an individual thickness of less than 1 μm. Bioactivity was confirmed using Artemia salina in vivo toxicity assay, while 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) reduction assay showed the good antioxidative potential of concentrations of 100 μg/ml and lower. Microdilution assay on several reference strains and clinical isolates showed promising antibacterial potential on Staphylococci, with minimal inhibitory concentration (MIC) being 800 μg/ml. Bioactive glass-based scaffolds were coated with ResNPs and characterized to confirm coating potential. All of the above make these particles a promising bioactive, easy-to-handle component in various biomaterial formulations.",
publisher = "SAGE Publications Ltd.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties",
pages = "122-133",
volume = "38",
number = "1",
doi = "10.1177/08853282231183109",
url = "https://hdl.handle.net/21.15107/rcub_dais_14705"
}

Tomić, N., Matić, T., Filipović, N., Mitić Ćulafić, D., Boccacccini, A. R.,& Stevanović, M.. (2023). Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications
SAGE Publications Ltd.., 38(1), 122-133.
https://doi.org/10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_14705

Tomić N, Matić T, Filipović N, Mitić Ćulafić D, Boccacccini AR, Stevanović M. Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties. in Journal of Biomaterials Applications. 2023;38(1):122-133.
doi:10.1177/08853282231183109
https://hdl.handle.net/21.15107/rcub_dais_14705 .

Tomić, Nina, Matić, Tamara, Filipović, Nenad, Mitić Ćulafić, Dragana, Boccacccini, Aldo R., Stevanović, Magdalena, "Synthesis and characterization of innovative resveratrol nanobelt-like particles and assessment of their bioactivity, antioxidative and antibacterial properties" in Journal of Biomaterials Applications, 38, no. 1 (2023):122-133,
https://doi.org/10.1177/08853282231183109 .,
https://hdl.handle.net/21.15107/rcub_dais_14705 .

TY  - JOUR
AU  - Jovanović, Marina
AU  - Vojvodić, Petar
AU  - Tenji, Dina
AU  - Tomić, Nina
AU  - Nešić, Jovana
AU  - Mitić-Ćulafić, Dragana
AU  - Miočinović, Jelena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14876
AB  - Fermented foods containing psychobiotics are of growing interest among food scientists. Human-derived Limosilactobacillus reuteri DSM 17938, a gut symbiont and potential psychobiotic strain, has been shown to exhibit the following health benefits: anti-inflammation and GABA-production capacity, as well as modulation of pathogen and cancer cell growth. The aim of this research was to develop an acid-coagulated fresh soft quark-type cheese, fermented with L. reuteri DSM 17938, with enhanced bioactivity, sensory acceptability, and overall likeability. Psychobiotic-containing cheeses represent the food of a new generation, so it is of great importance to gain the trust of the consumers. To develop a familiar taste, cheese samples were enriched with mushroom powders of Agaricus bisporus and Pleurotus ostreatus. A high abundance of lactic acid bacteria was maintained in all cheese samples (>log 7.64 CFU/mL), while cheese extracts exhibited cytotoxicity to colon cancer cell line HCT116 (up to 30.96%) in vitro. Additionally, cheese samples provided a favorable medium for the growth of the probiotic Escherichia coli Nissle 1917 (>log 7.11 CFU/mL). Sensory evaluation revealed high scores for all samples (up to 97.21% of maximum overall quality). The survey conducted in this study offered insights into consumer willingness to try products containing psychobiotics. This study demonstrates the potential for the successful development of fermented food products with L. reuteri DSM 17938, which exhibits all the desired traits that consumers may receive well. Further research is required to explore the potential health benefits of these innovative food products.
PB  - Basel : MDPI AG
T2  - Fermentation
T1  - Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability
SP  - 745
VL  - 9
IS  - 8
DO  - 10.3390/fermentation9080745
UR  - https://hdl.handle.net/21.15107/rcub_dais_14876
ER  - 

@article{
author = "Jovanović, Marina and Vojvodić, Petar and Tenji, Dina and Tomić, Nina and Nešić, Jovana and Mitić-Ćulafić, Dragana and Miočinović, Jelena",
year = "2023",
abstract = "Fermented foods containing psychobiotics are of growing interest among food scientists. Human-derived Limosilactobacillus reuteri DSM 17938, a gut symbiont and potential psychobiotic strain, has been shown to exhibit the following health benefits: anti-inflammation and GABA-production capacity, as well as modulation of pathogen and cancer cell growth. The aim of this research was to develop an acid-coagulated fresh soft quark-type cheese, fermented with L. reuteri DSM 17938, with enhanced bioactivity, sensory acceptability, and overall likeability. Psychobiotic-containing cheeses represent the food of a new generation, so it is of great importance to gain the trust of the consumers. To develop a familiar taste, cheese samples were enriched with mushroom powders of Agaricus bisporus and Pleurotus ostreatus. A high abundance of lactic acid bacteria was maintained in all cheese samples (>log 7.64 CFU/mL), while cheese extracts exhibited cytotoxicity to colon cancer cell line HCT116 (up to 30.96%) in vitro. Additionally, cheese samples provided a favorable medium for the growth of the probiotic Escherichia coli Nissle 1917 (>log 7.11 CFU/mL). Sensory evaluation revealed high scores for all samples (up to 97.21% of maximum overall quality). The survey conducted in this study offered insights into consumer willingness to try products containing psychobiotics. This study demonstrates the potential for the successful development of fermented food products with L. reuteri DSM 17938, which exhibits all the desired traits that consumers may receive well. Further research is required to explore the potential health benefits of these innovative food products.",
publisher = "Basel : MDPI AG",
journal = "Fermentation",
title = "Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability",
pages = "745",
volume = "9",
number = "8",
doi = "10.3390/fermentation9080745",
url = "https://hdl.handle.net/21.15107/rcub_dais_14876"
}

Jovanović, M., Vojvodić, P., Tenji, D., Tomić, N., Nešić, J., Mitić-Ćulafić, D.,& Miočinović, J.. (2023). Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability. in Fermentation
Basel : MDPI AG., 9(8), 745.
https://doi.org/10.3390/fermentation9080745
https://hdl.handle.net/21.15107/rcub_dais_14876

Jovanović M, Vojvodić P, Tenji D, Tomić N, Nešić J, Mitić-Ćulafić D, Miočinović J. Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability. in Fermentation. 2023;9(8):745.
doi:10.3390/fermentation9080745
https://hdl.handle.net/21.15107/rcub_dais_14876 .

Jovanović, Marina, Vojvodić, Petar, Tenji, Dina, Tomić, Nina, Nešić, Jovana, Mitić-Ćulafić, Dragana, Miočinović, Jelena, "Cheese Fermented with Human-Derived Limosilactobacillus reuteri DSM 17938 and Mushroom Powders: A Novel Psychobiotic Food with Enhanced Bioactivity and Sensory Acceptability" in Fermentation, 9, no. 8 (2023):745,
https://doi.org/10.3390/fermentation9080745 .,
https://hdl.handle.net/21.15107/rcub_dais_14876 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Ganić, Tea
AU  - Klyagin, Sergey
AU  - Osmolovskiy, Alexander
AU  - Stevanović, Magdalena
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15635
AB  - Biomaterial implant contaminations are the most common nosocomial infections. Especially complicated to treat are the ones caused by microbial biofilms. Biofilms are structures comprised of surface-attached cells protected by self-produced polymeric matrix, and are present in more than 80% of persistent infections. Pseudomonas aeruginosa biofilms are exceptionally difficult to resolve, due to high capability of this bacteria for producing biofilm and acquiring antibiotic resistance. In order to prevent biofilm formation and reduce the unnecessary use of antibiotics, various nanomaterials and natural antibiofilm agents are being given more attention in tissue engineering in the last decade. Bioactive polyphenolic compounds have potential for use in biomaterials as multifunctional additives. Among polyphenols, resveratrol is one of the most known, but its’ use is limited by low bioavailability and difficulty of delivery. In our previous research, we created resveratrol nanobelt-like particles of shape convenient for handling and scaffold coating. Here we investigated their potential for inhibiting growth and biofilm formation of several strains of Pseudomonas aeruginosa. Microdilution assay and resazurin staining, as well as agar plating were used to estimate minimal inhibitory and minimal bactericidal concentration. MTT method was performed to more accurately measure the influence of various concentrations on bacterial growth. Crystal violet assay and RT PCR analysis determined the amount of biofilm formation and effect on gene expression. The effect varied among the strains, but most of the tested concentrations, including the lowest ones, led to at least 40% of inhibition of biofilm formation. Concentration of 800 μg/ml achieved almost 90% inhibition of biofilm formation in ATCC reference strain, indicating potential of this material for further use for such purpose in biomaterial design.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms
SP  - 8
EP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_dais_15635
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić Ćulafić, Dragana and Ganić, Tea and Klyagin, Sergey and Osmolovskiy, Alexander and Stevanović, Magdalena",
year = "2023",
abstract = "Biomaterial implant contaminations are the most common nosocomial infections. Especially complicated to treat are the ones caused by microbial biofilms. Biofilms are structures comprised of surface-attached cells protected by self-produced polymeric matrix, and are present in more than 80% of persistent infections. Pseudomonas aeruginosa biofilms are exceptionally difficult to resolve, due to high capability of this bacteria for producing biofilm and acquiring antibiotic resistance. In order to prevent biofilm formation and reduce the unnecessary use of antibiotics, various nanomaterials and natural antibiofilm agents are being given more attention in tissue engineering in the last decade. Bioactive polyphenolic compounds have potential for use in biomaterials as multifunctional additives. Among polyphenols, resveratrol is one of the most known, but its’ use is limited by low bioavailability and difficulty of delivery. In our previous research, we created resveratrol nanobelt-like particles of shape convenient for handling and scaffold coating. Here we investigated their potential for inhibiting growth and biofilm formation of several strains of Pseudomonas aeruginosa. Microdilution assay and resazurin staining, as well as agar plating were used to estimate minimal inhibitory and minimal bactericidal concentration. MTT method was performed to more accurately measure the influence of various concentrations on bacterial growth. Crystal violet assay and RT PCR analysis determined the amount of biofilm formation and effect on gene expression. The effect varied among the strains, but most of the tested concentrations, including the lowest ones, led to at least 40% of inhibition of biofilm formation. Concentration of 800 μg/ml achieved almost 90% inhibition of biofilm formation in ATCC reference strain, indicating potential of this material for further use for such purpose in biomaterial design.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms",
pages = "8-8",
url = "https://hdl.handle.net/21.15107/rcub_dais_15635"
}

Tomić, N., Filipović, N., Mitić Ćulafić, D., Ganić, T., Klyagin, S., Osmolovskiy, A.,& Stevanović, M.. (2023). Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 8-8.
https://hdl.handle.net/21.15107/rcub_dais_15635

Tomić N, Filipović N, Mitić Ćulafić D, Ganić T, Klyagin S, Osmolovskiy A, Stevanović M. Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:8-8.
https://hdl.handle.net/21.15107/rcub_dais_15635 .

Tomić, Nina, Filipović, Nenad, Mitić Ćulafić, Dragana, Ganić, Tea, Klyagin, Sergey, Osmolovskiy, Alexander, Stevanović, Magdalena, "Activity of resveratrol nanobelt-like particles against Pseudomonas aeruginosa biofilms" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):8-8,
https://hdl.handle.net/21.15107/rcub_dais_15635 .

TY  - CHAP
AU  - Filipović, Nenad
AU  - Tomić, Nina
AU  - Kuzmanović, Maja
AU  - Stevanović, Magdalena
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13184
AB  - One of the major issues related to medical devices and especially urinary stents are infections caused by different strains of bacteria and fungi, mainly in light of the recent rise in microbial resistance to existing antibiotics. Lately, it has been shown that nanomaterials could be superior alternatives to conventional antibiotics. Generally, nanoparticles are used for many applications in the biomedical field primarily due to the ability to adjust and control their physicochemical properties as well as their great reactivity due to the large surface-to-volume ratio. This has led to the formation of a new research field called nanomedicine which can be defined as the use of nanotechnology and nanomaterials in diagnostics, imaging, observing, prevention, control, and treatment of diseases. For example, coverings or coatings based on nanomaterials are now seen as a promising strategy for preventing or treating biofilms formation on healthcare kits, implants, and medical devices. Toxicity, inappropriate delivery, or degradation of conventionally used drugs for the treatment of infections may be avoided by using nanoparticles without or with encapsulated/immobilized active substances. Most of the materials which are used and examined for the preparation of the nanoparticles with encapsulated/immobilized active substances or smart reactive nanomaterials with antimicrobial effects are polymers, naturally derived antimicrobials, metal-based and non-metallic materials. This chapter provides an overview of the current state and future perspectives of the nanoparticle-based systems based on these materials for prevention, control, or elimination of biofilm-related infections on urinary stents. It also addresses manufacturing conditions indicating the huge potential for the improvement of existing and development of new promising stent solutions.
PB  - Cham : Springer International Publishing
T2  - Urinary Stents
T1  - Nanoparticles: Potential for Use to Prevent Infections
SP  - 325
EP  - 339
DO  - 10.1007/978-3-031-04484-7_26
UR  - https://hdl.handle.net/21.15107/rcub_dais_13184
ER  - 

@inbook{
editor = "Soria, Federico, Rako, Duje, de Graaf, Petra",
author = "Filipović, Nenad and Tomić, Nina and Kuzmanović, Maja and Stevanović, Magdalena",
year = "2022",
abstract = "One of the major issues related to medical devices and especially urinary stents are infections caused by different strains of bacteria and fungi, mainly in light of the recent rise in microbial resistance to existing antibiotics. Lately, it has been shown that nanomaterials could be superior alternatives to conventional antibiotics. Generally, nanoparticles are used for many applications in the biomedical field primarily due to the ability to adjust and control their physicochemical properties as well as their great reactivity due to the large surface-to-volume ratio. This has led to the formation of a new research field called nanomedicine which can be defined as the use of nanotechnology and nanomaterials in diagnostics, imaging, observing, prevention, control, and treatment of diseases. For example, coverings or coatings based on nanomaterials are now seen as a promising strategy for preventing or treating biofilms formation on healthcare kits, implants, and medical devices. Toxicity, inappropriate delivery, or degradation of conventionally used drugs for the treatment of infections may be avoided by using nanoparticles without or with encapsulated/immobilized active substances. Most of the materials which are used and examined for the preparation of the nanoparticles with encapsulated/immobilized active substances or smart reactive nanomaterials with antimicrobial effects are polymers, naturally derived antimicrobials, metal-based and non-metallic materials. This chapter provides an overview of the current state and future perspectives of the nanoparticle-based systems based on these materials for prevention, control, or elimination of biofilm-related infections on urinary stents. It also addresses manufacturing conditions indicating the huge potential for the improvement of existing and development of new promising stent solutions.",
publisher = "Cham : Springer International Publishing",
journal = "Urinary Stents",
booktitle = "Nanoparticles: Potential for Use to Prevent Infections",
pages = "325-339",
doi = "10.1007/978-3-031-04484-7_26",
url = "https://hdl.handle.net/21.15107/rcub_dais_13184"
}

Soria, F., Rako, D., de Graaf, P., Filipović, N., Tomić, N., Kuzmanović, M.,& Stevanović, M.. (2022). Nanoparticles: Potential for Use to Prevent Infections. in Urinary Stents
Cham : Springer International Publishing., 325-339.
https://doi.org/10.1007/978-3-031-04484-7_26
https://hdl.handle.net/21.15107/rcub_dais_13184

Soria F, Rako D, de Graaf P, Filipović N, Tomić N, Kuzmanović M, Stevanović M. Nanoparticles: Potential for Use to Prevent Infections. in Urinary Stents. 2022;:325-339.
doi:10.1007/978-3-031-04484-7_26
https://hdl.handle.net/21.15107/rcub_dais_13184 .

Soria, Federico, Rako, Duje, de Graaf, Petra, Filipović, Nenad, Tomić, Nina, Kuzmanović, Maja, Stevanović, Magdalena, "Nanoparticles: Potential for Use to Prevent Infections" in Urinary Stents (2022):325-339,
https://doi.org/10.1007/978-3-031-04484-7_26 .,
https://hdl.handle.net/21.15107/rcub_dais_13184 .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Tomić, Nina
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Stampfl, Juergen
AU  - Boccaccini, Aldo
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13579
AB  - Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
SP  - 1800
EP  - 1811
VL  - 36
IS  - 10
DO  - 10.1177/08853282211073731
UR  - https://hdl.handle.net/21.15107/rcub_dais_13579
ER  - 

@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccini, Aldo",
year = "2022",
abstract = "Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles",
pages = "1800-1811",
volume = "36",
number = "10",
doi = "10.1177/08853282211073731",
url = "https://hdl.handle.net/21.15107/rcub_dais_13579"
}

Stevanović, M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccini, A.. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications, 36(10), 1800-1811.
https://doi.org/10.1177/08853282211073731
https://hdl.handle.net/21.15107/rcub_dais_13579

Stevanović M, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccini A. Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications. 2022;36(10):1800-1811.
doi:10.1177/08853282211073731
https://hdl.handle.net/21.15107/rcub_dais_13579 .

Stevanović, Magdalena, Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccini, Aldo, "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles" in Journal of Biomaterials Applications, 36, no. 10 (2022):1800-1811,
https://doi.org/10.1177/08853282211073731 .,
https://hdl.handle.net/21.15107/rcub_dais_13579 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić Ćulafić, Dragana
AU  - Stevanović, Magdalena
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13501
AB  - Natural chemicals and earth elements are increasingly used in research as basis for novel materials intended for use in medicine. Among phytopharmaceuticals, more specifically polyphenols, resveratrol is known for its’ antioxidative, anticancer, antimicrobial, and other beneficial effects. Selenium, an essential trace element, is lately being recognized in nanoparticle form as less toxic and equally or more efficient than commercially available forms. The synergy of these two agents have not been shown until lately, when their synergistic antioxidative and gene expression effects were investigated for the purpose of treating Alcheimer disease. During our previous research, we have successfully synthesized pure resveratrol particles, as well as selenium nanoparticles (SeNPs). Both of them were separately investigated regarding their biological activities. The first step in evaluation of their possible synergistic antioxidative effect was obtaining the stable composite of these two materials. Synthesis parameters and processing methods were varied, and obtained suspensions assessed by their macroscopic and microscopic characteristics. The sample with both components homogeneously distributed in the particle form, was chosen for further experiments. Ultraviolet-visible (UV-Vis) spectrophotometry and Fourier transform infrared spectroscopy (FTIR) were used to characterize sample, and antioxidative activity (by DPPH reduction assay), and cytocompatibility (using MTT cytotoxicity assay) were additionally determined. Results showed improved cytocompatibility as compared to pure resveratrol particles, and preserved, significantly high antioxidative potential.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia
T1  - Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility
SP  - 5
EP  - 5
UR  - https://hdl.handle.net/21.15107/rcub_dais_13501
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić Ćulafić, Dragana and Stevanović, Magdalena",
year = "2022",
abstract = "Natural chemicals and earth elements are increasingly used in research as basis for novel materials intended for use in medicine. Among phytopharmaceuticals, more specifically polyphenols, resveratrol is known for its’ antioxidative, anticancer, antimicrobial, and other beneficial effects. Selenium, an essential trace element, is lately being recognized in nanoparticle form as less toxic and equally or more efficient than commercially available forms. The synergy of these two agents have not been shown until lately, when their synergistic antioxidative and gene expression effects were investigated for the purpose of treating Alcheimer disease. During our previous research, we have successfully synthesized pure resveratrol particles, as well as selenium nanoparticles (SeNPs). Both of them were separately investigated regarding their biological activities. The first step in evaluation of their possible synergistic antioxidative effect was obtaining the stable composite of these two materials. Synthesis parameters and processing methods were varied, and obtained suspensions assessed by their macroscopic and microscopic characteristics. The sample with both components homogeneously distributed in the particle form, was chosen for further experiments. Ultraviolet-visible (UV-Vis) spectrophotometry and Fourier transform infrared spectroscopy (FTIR) were used to characterize sample, and antioxidative activity (by DPPH reduction assay), and cytocompatibility (using MTT cytotoxicity assay) were additionally determined. Results showed improved cytocompatibility as compared to pure resveratrol particles, and preserved, significantly high antioxidative potential.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia",
title = "Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility",
pages = "5-5",
url = "https://hdl.handle.net/21.15107/rcub_dais_13501"
}

Tomić, N., Filipović, N., Mitić Ćulafić, D.,& Stevanović, M.. (2022). Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 5-5.
https://hdl.handle.net/21.15107/rcub_dais_13501

Tomić N, Filipović N, Mitić Ćulafić D, Stevanović M. Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia. 2022;:5-5.
https://hdl.handle.net/21.15107/rcub_dais_13501 .

Tomić, Nina, Filipović, Nenad, Mitić Ćulafić, Dragana, Stevanović, Magdalena, "Synthesis and characterization of composite resveratrol/selenium nanomaterial, and preliminary assessment of its’ antioxidative effect and biocompatibility" in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia (2022):5-5,
https://hdl.handle.net/21.15107/rcub_dais_13501 .

TY  - CONF
AU  - Tomić, Nina
AU  - Filipović, Nenad
AU  - Mitić-Ćulafić, Dragana
AU  - Đokić, Ana
AU  - Stevanović, Magdalena
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13605
AB  - Selenium is an especially important element for the functioning of antioxidative enzymes, making it one of the essential micronutrients. Although selenium is required in extremely small amounts, often it is lacking from the organism. However, inorganic and organic sources of selenium are known to possess considerable toxicity. Recently, the nanoparticle form of selenium has been shown to exhibit lesser toxicity, and also better antimicrobial and other biological effects, than selenium-containing compounds. Nanotechnology has already found application in many fields, for improving material properties for various purposes, among them, in the process of improving food characteristics. It is possible that selenium nanoparticles can be applied as antimicrobial agents in food, and there has been growing interest in this area of research. We have synthesized selenium nanoparticles (SeNPs) by chemical reduction method, using sodium selenite, ascorbic acid, and bovine serum albumin. The antimicrobial activity of these particles has been assessed by minimum inhibitory concentration assay and colony-forming units count, on Listeria monocytogenes and Salmonella enterica, which are common bacterial food contaminants. As intestinal microbiota, especially probiotic bacteria, have been shown to play important role in organism functioning, we also tested how SeNPs influence Lactobacillus rhamosus and Lactobacillus plantarum. To evaluate cytotoxicity, we used MTT assay on the human intestinal cell line (HT-29). Antioxidative properties were assessed by DPPH assay. SeNPs showed higher antibacterial activity on pathogenic bacteria than on probiotic strains. Cytotoxic effect was present only in the highest tested concentrations. The antioxidative activity was considerable, resulting in above 80% reduction of DPPH free radical, at 1 g/ml of SeNPs. Based on these results, SeNPs are a promising subject for further research in developing materials for food packaging or as antimicrobial selenium additives.
PB  - Novi Sad : University of Novi Sad, Faculty of Technology
C3  - Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
T1  - Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells
SP  - 129
EP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_dais_13605
ER  - 

@conference{
author = "Tomić, Nina and Filipović, Nenad and Mitić-Ćulafić, Dragana and Đokić, Ana and Stevanović, Magdalena",
year = "2022",
abstract = "Selenium is an especially important element for the functioning of antioxidative enzymes, making it one of the essential micronutrients. Although selenium is required in extremely small amounts, often it is lacking from the organism. However, inorganic and organic sources of selenium are known to possess considerable toxicity. Recently, the nanoparticle form of selenium has been shown to exhibit lesser toxicity, and also better antimicrobial and other biological effects, than selenium-containing compounds. Nanotechnology has already found application in many fields, for improving material properties for various purposes, among them, in the process of improving food characteristics. It is possible that selenium nanoparticles can be applied as antimicrobial agents in food, and there has been growing interest in this area of research. We have synthesized selenium nanoparticles (SeNPs) by chemical reduction method, using sodium selenite, ascorbic acid, and bovine serum albumin. The antimicrobial activity of these particles has been assessed by minimum inhibitory concentration assay and colony-forming units count, on Listeria monocytogenes and Salmonella enterica, which are common bacterial food contaminants. As intestinal microbiota, especially probiotic bacteria, have been shown to play important role in organism functioning, we also tested how SeNPs influence Lactobacillus rhamosus and Lactobacillus plantarum. To evaluate cytotoxicity, we used MTT assay on the human intestinal cell line (HT-29). Antioxidative properties were assessed by DPPH assay. SeNPs showed higher antibacterial activity on pathogenic bacteria than on probiotic strains. Cytotoxic effect was present only in the highest tested concentrations. The antioxidative activity was considerable, resulting in above 80% reduction of DPPH free radical, at 1 g/ml of SeNPs. Based on these results, SeNPs are a promising subject for further research in developing materials for food packaging or as antimicrobial selenium additives.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Technology",
journal = "Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad",
title = "Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells",
pages = "129-129",
url = "https://hdl.handle.net/21.15107/rcub_dais_13605"
}

Tomić, N., Filipović, N., Mitić-Ćulafić, D., Đokić, A.,& Stevanović, M.. (2022). Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad
Novi Sad : University of Novi Sad, Faculty of Technology., 129-129.
https://hdl.handle.net/21.15107/rcub_dais_13605

Tomić N, Filipović N, Mitić-Ćulafić D, Đokić A, Stevanović M. Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells. in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad. 2022;:129-129.
https://hdl.handle.net/21.15107/rcub_dais_13605 .

Tomić, Nina, Filipović, Nenad, Mitić-Ćulafić, Dragana, Đokić, Ana, Stevanović, Magdalena, "Effects of selenium nanoparticles on pathogenic and probiotic food bacteria, and their influence on human intestinal cells" in Book of abstracts / 2nd International Conference on Advanced Production and Processing, 20th-22nd October 2022, Novi Sad (2022):129-129,
https://hdl.handle.net/21.15107/rcub_dais_13605 .

TY  - CONF
AU  - Томић, Нина
AU  - Филиповић, Ненад
AU  - Гајић, Ина
AU  - Стевановић, Магдалена
AU  - Митић-Ћулафић, Драгана
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13606
AB  - Биофилм као животна форма представља један од најважнијих фактора вируленције који је заступљен у 80-90% инфекција, а посебно на материјалима који се користе у ортопедији. Бактерије у биофилму су вишеструко отпорније на антибиотике и спољашње факторе, те је превенција развоја биофилма некада једини ефикасан метод спречавања појаве и ширења инфекције. У последње време интензивно се проучавају наноструктуирани материјали отпорни на колонизацију бактерија и развој биофилма. Селен је један од елемената који у својој наноформи показује добре биолошке ефекте, а ресвератрол је популарни полифенол чији се потенцијал за примену у медицини интензивно истражује. Добијањем обе ове компоненте у нано- облицима, отварају се даље могућности комбиновања и стварања композитног материјала са побољшаним особинама, за примену у превенцији биофилма. Ресвератрол и наночестице селена појединачно показују добар, али недовољно истражен антимикробни потенцијал. Након њиховог спајања испитали смо ефекат добијеног композита у спречавању формирања биофилма код више бактеријских врста. Концентрације композита од 6% (и ниже) су доводиле до инхибиције формирања биофилма од најмање 20% код већине тестираних бактерија. Посебно се истиче добар антибиофилм ефекат на сојевима P. aeruginosa, где је на тестираним концентрацијама уочена инхибиција формирања биофилма у опсегу од 20-60%. Такође, антибиофилм ефекат композита је у већини случајева бољи у односу на саме честице у истим концентрацијама.
PB  - Београд : Српско биолошко друштво
C3  - Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
T1  - Антибиофилм активност нанокомпозита на бази ресвератрола и селена
SP  - 252
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_dais_13606
ER  - 

@conference{
author = "Томић, Нина and Филиповић, Ненад and Гајић, Ина and Стевановић, Магдалена and Митић-Ћулафић, Драгана",
year = "2022",
abstract = "Биофилм као животна форма представља један од најважнијих фактора вируленције који је заступљен у 80-90% инфекција, а посебно на материјалима који се користе у ортопедији. Бактерије у биофилму су вишеструко отпорније на антибиотике и спољашње факторе, те је превенција развоја биофилма некада једини ефикасан метод спречавања појаве и ширења инфекције. У последње време интензивно се проучавају наноструктуирани материјали отпорни на колонизацију бактерија и развој биофилма. Селен је један од елемената који у својој наноформи показује добре биолошке ефекте, а ресвератрол је популарни полифенол чији се потенцијал за примену у медицини интензивно истражује. Добијањем обе ове компоненте у нано- облицима, отварају се даље могућности комбиновања и стварања композитног материјала са побољшаним особинама, за примену у превенцији биофилма. Ресвератрол и наночестице селена појединачно показују добар, али недовољно истражен антимикробни потенцијал. Након њиховог спајања испитали смо ефекат добијеног композита у спречавању формирања биофилма код више бактеријских врста. Концентрације композита од 6% (и ниже) су доводиле до инхибиције формирања биофилма од најмање 20% код већине тестираних бактерија. Посебно се истиче добар антибиофилм ефекат на сојевима P. aeruginosa, где је на тестираним концентрацијама уочена инхибиција формирања биофилма у опсегу од 20-60%. Такође, антибиофилм ефекат композита је у већини случајева бољи у односу на саме честице у истим концентрацијама.",
publisher = "Београд : Српско биолошко друштво",
journal = "Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.",
title = "Антибиофилм активност нанокомпозита на бази ресвератрола и селена",
pages = "252-252",
url = "https://hdl.handle.net/21.15107/rcub_dais_13606"
}

Томић, Н., Филиповић, Н., Гајић, И., Стевановић, М.,& Митић-Ћулафић, Д.. (2022). Антибиофилм активност нанокомпозита на бази ресвератрола и селена. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
Београд : Српско биолошко друштво., 252-252.
https://hdl.handle.net/21.15107/rcub_dais_13606

Томић Н, Филиповић Н, Гајић И, Стевановић М, Митић-Ћулафић Д. Антибиофилм активност нанокомпозита на бази ресвератрола и селена. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.. 2022;:252-252.
https://hdl.handle.net/21.15107/rcub_dais_13606 .

Томић, Нина, Филиповић, Ненад, Гајић, Ина, Стевановић, Магдалена, Митић-Ћулафић, Драгана, "Антибиофилм активност нанокомпозита на бази ресвератрола и селена" in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022. (2022):252-252,
https://hdl.handle.net/21.15107/rcub_dais_13606 .

TY  - CONF
AU  - Tomić, Nina
AU  - Kuzmanović, Maja
AU  - Mitić Ćulafić, Dragana
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12283
AB  - Resveratrol, polyphenol from stilbene family, has demonstrated beneficial effects on human health in numerous studies. Among these effects are cardioprotective, anti-cancer, neuroprotective, antimicrobial and other. In vivo, application of this phytochemical often lacks significant results. This is mainly because of its poor bioavailability, caused by low water solubility, sensitivity to light, oxygen and pH change, and high reactivity. Micro- and nanoformulations of resveratrol have been described to highly improve bioavailabiliy. However, there are limitations to this approach, such as demanding synthesis process, necessity of expensive or toxic chemicals, or poor loading capacity. We have used a simple physicochemical solvent-non solvent method to synthetise additive-free, stabile and uniform elongated resveratrol micro- and nanoparticles (RES-particles). The drying conditions were varied to examine their influence on the morphological characteristics of resveratrol particles. Differential effects of drying at ambient, low, and elevated temperature as well as effects of centrifugation and stability of RES-particle suspension during the time were documented. RES-particles were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), ultraviolet-visible (UV-Vis) spectrophotometry, Fourier transform infrared spectroscopy (FTIR) and optical microscopy. Biocompatibility of RESparticles was preliminary assessed through MTT toxicity assay.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia
T1  - Additive-free resveratrol micro- and nanoparticles and assessment of their toxicity
SP  - 4
EP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_dais_12283
ER  - 

@conference{
author = "Tomić, Nina and Kuzmanović, Maja and Mitić Ćulafić, Dragana and Stevanović, Magdalena",
year = "2021",
abstract = "Resveratrol, polyphenol from stilbene family, has demonstrated beneficial effects on human health in numerous studies. Among these effects are cardioprotective, anti-cancer, neuroprotective, antimicrobial and other. In vivo, application of this phytochemical often lacks significant results. This is mainly because of its poor bioavailability, caused by low water solubility, sensitivity to light, oxygen and pH change, and high reactivity. Micro- and nanoformulations of resveratrol have been described to highly improve bioavailabiliy. However, there are limitations to this approach, such as demanding synthesis process, necessity of expensive or toxic chemicals, or poor loading capacity. We have used a simple physicochemical solvent-non solvent method to synthetise additive-free, stabile and uniform elongated resveratrol micro- and nanoparticles (RES-particles). The drying conditions were varied to examine their influence on the morphological characteristics of resveratrol particles. Differential effects of drying at ambient, low, and elevated temperature as well as effects of centrifugation and stability of RES-particle suspension during the time were documented. RES-particles were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), ultraviolet-visible (UV-Vis) spectrophotometry, Fourier transform infrared spectroscopy (FTIR) and optical microscopy. Biocompatibility of RESparticles was preliminary assessed through MTT toxicity assay.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia",
title = "Additive-free resveratrol micro- and nanoparticles and assessment of their toxicity",
pages = "4-4",
url = "https://hdl.handle.net/21.15107/rcub_dais_12283"
}

Tomić, N., Kuzmanović, M., Mitić Ćulafić, D.,& Stevanović, M.. (2021). Additive-free resveratrol micro- and nanoparticles and assessment of their toxicity. in Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 4-4.
https://hdl.handle.net/21.15107/rcub_dais_12283

Tomić N, Kuzmanović M, Mitić Ćulafić D, Stevanović M. Additive-free resveratrol micro- and nanoparticles and assessment of their toxicity. in Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia. 2021;:4-4.
https://hdl.handle.net/21.15107/rcub_dais_12283 .

Tomić, Nina, Kuzmanović, Maja, Mitić Ćulafić, Dragana, Stevanović, Magdalena, "Additive-free resveratrol micro- and nanoparticles and assessment of their toxicity" in Program and the Book of abstracts / Nineteenth Young Researchers' Conference Materials Science and Engineering, December 1-3, 2021, Belgrade, Serbia (2021):4-4,
https://hdl.handle.net/21.15107/rcub_dais_12283 .

TY  - CONF
AU  - Đukanović, S.
AU  - Cvetković, S.
AU  - Ganić, T.
AU  - Nikolić, B.
AU  - Tomić, Nina
AU  - Kekić, D.
AU  - Mitić Ćulafić, D.
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12380
AB  - The harmful effects that antibiotics may have on human health and increased resistance of microorganisms on antibiotics made pharmaceutical companies look for new alternatives among medicinal and aromatic plants. Recently, as a new strategy to enhance antimicrobial activities of commercial antibiotics, the combination of plant extracts and antibiotics is developing. The aim of the study was to examine the potential synergistic effect of plant extracts with streptomycin. The minimum inhibitory concentration of two extracts obtained from traditional medicinal plants, Alchemilla vulgaris and Frangula alnus, was determined by the microdilution method. The effect of a mixture of extracts and antibiotics was tested with checkerboard assay and confirmed by Time kill assay. The most sensitive strain was E. coli ATCC 25922.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - Antibacterial activity of aqueous-ethanolic extracts of Alchemilla vulgaris and Frangula alnus combined with streptomycin
SP  - 176
EP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_dais_12380
ER  - 

@conference{
author = "Đukanović, S. and Cvetković, S. and Ganić, T. and Nikolić, B. and Tomić, Nina and Kekić, D. and Mitić Ćulafić, D.",
year = "2021",
abstract = "The harmful effects that antibiotics may have on human health and increased resistance of microorganisms on antibiotics made pharmaceutical companies look for new alternatives among medicinal and aromatic plants. Recently, as a new strategy to enhance antimicrobial activities of commercial antibiotics, the combination of plant extracts and antibiotics is developing. The aim of the study was to examine the potential synergistic effect of plant extracts with streptomycin. The minimum inhibitory concentration of two extracts obtained from traditional medicinal plants, Alchemilla vulgaris and Frangula alnus, was determined by the microdilution method. The effect of a mixture of extracts and antibiotics was tested with checkerboard assay and confirmed by Time kill assay. The most sensitive strain was E. coli ATCC 25922.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "Antibacterial activity of aqueous-ethanolic extracts of Alchemilla vulgaris and Frangula alnus combined with streptomycin",
pages = "176-179",
url = "https://hdl.handle.net/21.15107/rcub_dais_12380"
}

Đukanović, S., Cvetković, S., Ganić, T., Nikolić, B., Tomić, N., Kekić, D.,& Mitić Ćulafić, D.. (2021). Antibacterial activity of aqueous-ethanolic extracts of Alchemilla vulgaris and Frangula alnus combined with streptomycin. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 176-179.
https://hdl.handle.net/21.15107/rcub_dais_12380

Đukanović S, Cvetković S, Ganić T, Nikolić B, Tomić N, Kekić D, Mitić Ćulafić D. Antibacterial activity of aqueous-ethanolic extracts of Alchemilla vulgaris and Frangula alnus combined with streptomycin. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:176-179.
https://hdl.handle.net/21.15107/rcub_dais_12380 .

Đukanović, S., Cvetković, S., Ganić, T., Nikolić, B., Tomić, Nina, Kekić, D., Mitić Ćulafić, D., "Antibacterial activity of aqueous-ethanolic extracts of Alchemilla vulgaris and Frangula alnus combined with streptomycin" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):176-179,
https://hdl.handle.net/21.15107/rcub_dais_12380 .