TY  - JOUR
AU  - Mitranić, Ivana
AU  - Stevanović, Magdalena
AU  - Nedeljković, B.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2009
UR  - https://dais.sanu.ac.rs/123456789/3476
AB  - In this work, horseradish peroxidase (HRP), a model protein, was encapsulated by the precipitation method with additional step of homogenization, using polymer poly(D,L-lactide) (PDLLA). The purpose of this study was to expand the application of a modified precipitation method to HRP-loaded PDLLA spheres and to find optimal conditions for preparing HRP-loaded PDLLA spheres with the best properties, with size under 1000 nm. Influence of process variables on the size and morphology of the particles has been studied. Size of the spheres depends on homogenization speed, co-solvent selection, chloroform to water ratio and PVA concentration. Particle size was ranging between 460 nm and 1130 nm, according to the preparation conditions. The optimized conditions yielded nanospheres with 46% encapsulation efficiency and average diameter of 530 nm. A particle size under 1000 nm enables intravascular injection and is also desirable for intramuscular and subcutaneous administration, minimizing possible irritant reactions. It was found that modified precipitation method was widely applicable to HRP-loaded PDLLA spheres, by choosing optimal preparation conditions. Copyright © 2009 American Scientific Publishers All rights reserved.
PB  - American Scientific Publishers
T2  - Journal of Bionanoscience
T1  - Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres
SP  - 22
EP  - 32
VL  - 3
IS  - 1
DO  - 10.1166/jbns.2009.1005
UR  - https://hdl.handle.net/21.15107/rcub_dais_3476
ER  - 

@article{
author = "Mitranić, Ivana and Stevanović, Magdalena and Nedeljković, B. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2009",
abstract = "In this work, horseradish peroxidase (HRP), a model protein, was encapsulated by the precipitation method with additional step of homogenization, using polymer poly(D,L-lactide) (PDLLA). The purpose of this study was to expand the application of a modified precipitation method to HRP-loaded PDLLA spheres and to find optimal conditions for preparing HRP-loaded PDLLA spheres with the best properties, with size under 1000 nm. Influence of process variables on the size and morphology of the particles has been studied. Size of the spheres depends on homogenization speed, co-solvent selection, chloroform to water ratio and PVA concentration. Particle size was ranging between 460 nm and 1130 nm, according to the preparation conditions. The optimized conditions yielded nanospheres with 46% encapsulation efficiency and average diameter of 530 nm. A particle size under 1000 nm enables intravascular injection and is also desirable for intramuscular and subcutaneous administration, minimizing possible irritant reactions. It was found that modified precipitation method was widely applicable to HRP-loaded PDLLA spheres, by choosing optimal preparation conditions. Copyright © 2009 American Scientific Publishers All rights reserved.",
publisher = "American Scientific Publishers",
journal = "Journal of Bionanoscience",
title = "Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres",
pages = "22-32",
volume = "3",
number = "1",
doi = "10.1166/jbns.2009.1005",
url = "https://hdl.handle.net/21.15107/rcub_dais_3476"
}

Mitranić, I., Stevanović, M., Nedeljković, B., Ignjatović, N.,& Uskoković, D.. (2009). Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres. in Journal of Bionanoscience
American Scientific Publishers., 3(1), 22-32.
https://doi.org/10.1166/jbns.2009.1005
https://hdl.handle.net/21.15107/rcub_dais_3476

Mitranić I, Stevanović M, Nedeljković B, Ignjatović N, Uskoković D. Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres. in Journal of Bionanoscience. 2009;3(1):22-32.
doi:10.1166/jbns.2009.1005
https://hdl.handle.net/21.15107/rcub_dais_3476 .

Mitranić, Ivana, Stevanović, Magdalena, Nedeljković, B., Ignjatović, Nenad, Uskoković, Dragan, "Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres" in Journal of Bionanoscience, 3, no. 1 (2009):22-32,
https://doi.org/10.1166/jbns.2009.1005 .,
https://hdl.handle.net/21.15107/rcub_dais_3476 .