TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15642
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that are easy to obtain from any kind of discarded teeth and have the ability to differentiate into several cell types which makes them very popular in researches regarding regenerative dentistry. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry and they become very attractive tool for the treatment of bone tissue defects but also as a carriers of bioactive molecules. Before clinical application, thorough preclinical studies should be conducted regarding biocompatibility and biofunctionality of synthesized nanomaterials. Therefore, the aim of this study was to examine the influence of synthesized nanomaterial, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), on the proliferation of DPSCs during osteogenic differentiation in cell culture in vitro. We isolated the cells from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were incubated with two concentrations of CP/PLGA nanoparticles in standard cell culture media and media for osteogenic differentiation. Cell proliferation rate was assessed using MTT and Crystal violet test after one, three and seven days of cell culturing. The results showed time- and concentration-dependent differences in DPSCs proliferation in the presence of nanomaterial. Differences in cell proliferation were noticed between osteogenic and standard cell culture media as well as in results obtained by performed tests. Increased cell proliferation was observed over time in both media but this effect was dependent on nanomaterial concentration. Calcium phosphate-based nanomaterials and DPSCs, based on observed cell materials interactions, could be a promising tool in bone tissue regeneration and further studies using in vitro as well as in vivo models should be conducted to fully unravel all of its potential.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_dais_15642
ER  - 

@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2023",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that are easy to obtain from any kind of discarded teeth and have the ability to differentiate into several cell types which makes them very popular in researches regarding regenerative dentistry. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry and they become very attractive tool for the treatment of bone tissue defects but also as a carriers of bioactive molecules. Before clinical application, thorough preclinical studies should be conducted regarding biocompatibility and biofunctionality of synthesized nanomaterials. Therefore, the aim of this study was to examine the influence of synthesized nanomaterial, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), on the proliferation of DPSCs during osteogenic differentiation in cell culture in vitro. We isolated the cells from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were incubated with two concentrations of CP/PLGA nanoparticles in standard cell culture media and media for osteogenic differentiation. Cell proliferation rate was assessed using MTT and Crystal violet test after one, three and seven days of cell culturing. The results showed time- and concentration-dependent differences in DPSCs proliferation in the presence of nanomaterial. Differences in cell proliferation were noticed between osteogenic and standard cell culture media as well as in results obtained by performed tests. Increased cell proliferation was observed over time in both media but this effect was dependent on nanomaterial concentration. Calcium phosphate-based nanomaterials and DPSCs, based on observed cell materials interactions, could be a promising tool in bone tissue regeneration and further studies using in vitro as well as in vivo models should be conducted to fully unravel all of its potential.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_dais_15642"
}

Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2023). Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 11-11.
https://hdl.handle.net/21.15107/rcub_dais_15642

Tomić M, Stojanović S, Ignjatović N, Najman S. Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:11-11.
https://hdl.handle.net/21.15107/rcub_dais_15642 .

Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Influence of synthesized calcium phosphate-based nanomaterial on proliferation of dental pulp stem cells in various in vitro conditions" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):11-11,
https://hdl.handle.net/21.15107/rcub_dais_15642 .

TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/6969
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
T1  - Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro
SP  - 4
EP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_dais_6969
ER  - 

@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2019",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia",
title = "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro",
pages = "4-4",
url = "https://hdl.handle.net/21.15107/rcub_dais_6969"
}

Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2019). Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 4-4.
https://hdl.handle.net/21.15107/rcub_dais_6969

Tomić M, Stojanović S, Ignjatović N, Najman S. Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:4-4.
https://hdl.handle.net/21.15107/rcub_dais_6969 .

Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro" in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia (2019):4-4,
https://hdl.handle.net/21.15107/rcub_dais_6969 .