Repnik, U.

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  • Repnik, U. (2)
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Author's Bibliography

Antibacterial activity and cytotoxicity of hydroxyapatite/silver nanocomposites synthesized by a sonochemical approach

Vukomanović, Marija; Repnik, U.; Škapin, Srečo Davor; Uskoković, Dragan; Suvorov, Danilo

(Belgrade : Materials Research Society of Serbia, 2011)

TY  - CONF
AU  - Vukomanović, Marija
AU  - Repnik, U.
AU  - Škapin, Srečo Davor
AU  - Uskoković, Dragan
AU  - Suvorov, Danilo
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/689
AB  - Hydroxyapatite, as a major constituent of the inorganic part of natural human bones, is a natural choice and one of the most frequently applied materials for the reparation and regeneration of bone tissue. The formation of composites based on HAp, the incorporation of foreign ions within its structure and/or the functionalization of their surfaces allow improvements to the morphological, structural and surface properties. Such improvements can be utilized to increase the material quality for applications in dentistry and orthopedics as well to extend the potential applicability in other fields of biomedicine. Hydroxyapatite/silver (HAp/Ag) nanocomposite particles were formed using the sonochemical method. Particles were formed from HAp rods with silver (present in the form of ions) incorporated within the apatite structure and/or metallic particles, embedded into apatite rods and metallic particles attached to the surfaces of these rods. The so obtained materials were tested for antibacterial activity. The p presence of the bacterial growth-inhibition zone around the discs made of composite material and the fluorescence dye detection on the surface of the HAp/Ag composite (corresponding to the labeled live and dead bacteria) proved that the composite was active against both Gram-positive and Gram-negative bacteria. In addition, it was determined that the minimal inhibition concentration (MIC), i.e., the minimal concentration of HAp/Ag composite, which is able to inhibit the growth of bacteria, was higher for Grampositive (MIC = 0.7-0.8 mg/ml) when compared to Gram-negative (MIC = 0.6-0.7 mg/ml) bacteria. The same effect was determined during the investigation of the minimal bactericidal concentration (MBC) of the HAp/Ag composite against the same representatives of Grampositive and Gram-negative bacteria. The obtained concentrations of the HAp/Ag composite which were able to manifest the bactericidal effect were used for an investigation of the toxicity against the representatives of healthy and cancer-modified cell lines. The toxicity of a composite was investigated in vitro and for that purpose the IMR-90 human lung embryonic fibroblast and U-2 OS human osteosarcoma cell lines were applied. In the presentation the antibacterial activity of the HAp/Ag composite and its toxicity against human cells will be discussed.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
T1  - Antibacterial activity and cytotoxicity of hydroxyapatite/silver nanocomposites synthesized by a sonochemical approach
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_dais_689
ER  - 
@conference{
author = "Vukomanović, Marija and Repnik, U. and Škapin, Srečo Davor and Uskoković, Dragan and Suvorov, Danilo",
year = "2011",
abstract = "Hydroxyapatite, as a major constituent of the inorganic part of natural human bones, is a natural choice and one of the most frequently applied materials for the reparation and regeneration of bone tissue. The formation of composites based on HAp, the incorporation of foreign ions within its structure and/or the functionalization of their surfaces allow improvements to the morphological, structural and surface properties. Such improvements can be utilized to increase the material quality for applications in dentistry and orthopedics as well to extend the potential applicability in other fields of biomedicine. Hydroxyapatite/silver (HAp/Ag) nanocomposite particles were formed using the sonochemical method. Particles were formed from HAp rods with silver (present in the form of ions) incorporated within the apatite structure and/or metallic particles, embedded into apatite rods and metallic particles attached to the surfaces of these rods. The so obtained materials were tested for antibacterial activity. The p presence of the bacterial growth-inhibition zone around the discs made of composite material and the fluorescence dye detection on the surface of the HAp/Ag composite (corresponding to the labeled live and dead bacteria) proved that the composite was active against both Gram-positive and Gram-negative bacteria. In addition, it was determined that the minimal inhibition concentration (MIC), i.e., the minimal concentration of HAp/Ag composite, which is able to inhibit the growth of bacteria, was higher for Grampositive (MIC = 0.7-0.8 mg/ml) when compared to Gram-negative (MIC = 0.6-0.7 mg/ml) bacteria. The same effect was determined during the investigation of the minimal bactericidal concentration (MBC) of the HAp/Ag composite against the same representatives of Grampositive and Gram-negative bacteria. The obtained concentrations of the HAp/Ag composite which were able to manifest the bactericidal effect were used for an investigation of the toxicity against the representatives of healthy and cancer-modified cell lines. The toxicity of a composite was investigated in vitro and for that purpose the IMR-90 human lung embryonic fibroblast and U-2 OS human osteosarcoma cell lines were applied. In the presentation the antibacterial activity of the HAp/Ag composite and its toxicity against human cells will be discussed.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts",
title = "Antibacterial activity and cytotoxicity of hydroxyapatite/silver nanocomposites synthesized by a sonochemical approach",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_dais_689"
}
Vukomanović, M., Repnik, U., Škapin, S. D., Uskoković, D.,& Suvorov, D.. (2011). Antibacterial activity and cytotoxicity of hydroxyapatite/silver nanocomposites synthesized by a sonochemical approach. in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 69-69.
https://hdl.handle.net/21.15107/rcub_dais_689
Vukomanović M, Repnik U, Škapin SD, Uskoković D, Suvorov D. Antibacterial activity and cytotoxicity of hydroxyapatite/silver nanocomposites synthesized by a sonochemical approach. in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts. 2011;:69-69.
https://hdl.handle.net/21.15107/rcub_dais_689 .
Vukomanović, Marija, Repnik, U., Škapin, Srečo Davor, Uskoković, Dragan, Suvorov, Danilo, "Antibacterial activity and cytotoxicity of hydroxyapatite/silver nanocomposites synthesized by a sonochemical approach" in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts (2011):69-69,
https://hdl.handle.net/21.15107/rcub_dais_689 .

Antibacterial activity of hydroxyapatite/silver nanocomposite

Vukomanović, Marija; Repnik, U.; Zavašnik, T.; Škapin, Srečo Davor; Uskoković, Dragan; Suvorov, Danilo

(Belgrade : Institute of Technical Sciences of SASA, 2010)

TY  - CONF
AU  - Vukomanović, Marija
AU  - Repnik, U.
AU  - Zavašnik, T.
AU  - Škapin, Srečo Davor
AU  - Uskoković, Dragan
AU  - Suvorov, Danilo
PY  - 2010
UR  - http://dais.sanu.ac.rs/123456789/3452
AB  - Hydroxyapatite containing silver nanoparticles (HAp/Ag) was synthesized by homogeneous sonochemical metod. Morphology of obtained particles was formed of micrometer-sized rod-like HAp with nanosized silver sphere-like particles attached to their surface. So obtained material was tested for interaction with bacteria. For that purpose composites with three different contents of silver were prepared and their interactions with two different types of bacteria were studied. Escherichia coli was used as a representative of Gram negative while Staphylococcus aureus was applied as a representative of Gram positive bacteria. In the case of E. coli wider inhibition zone without presence of bacteria and with a layer of inactive bacteria near the surface of material was obtained. In this case obtained antibacterial effect was not concentration dependant. In the case of S. aureus, inhibition zone was narrower with the presence of modified bacteria in inhibition zone. Obtained antibacterial response pronounced dependence on concentration of silver within composite. According to results achieved with this investigation we concluded that HAp/Ag composite is effective against both, Gram positive and Gram negative bacteria and shows stronger activity against E. coli.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia
T1  - Antibacterial activity of hydroxyapatite/silver nanocomposite
SP  - 15
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_dais_3452
ER  - 
@conference{
author = "Vukomanović, Marija and Repnik, U. and Zavašnik, T. and Škapin, Srečo Davor and Uskoković, Dragan and Suvorov, Danilo",
year = "2010",
abstract = "Hydroxyapatite containing silver nanoparticles (HAp/Ag) was synthesized by homogeneous sonochemical metod. Morphology of obtained particles was formed of micrometer-sized rod-like HAp with nanosized silver sphere-like particles attached to their surface. So obtained material was tested for interaction with bacteria. For that purpose composites with three different contents of silver were prepared and their interactions with two different types of bacteria were studied. Escherichia coli was used as a representative of Gram negative while Staphylococcus aureus was applied as a representative of Gram positive bacteria. In the case of E. coli wider inhibition zone without presence of bacteria and with a layer of inactive bacteria near the surface of material was obtained. In this case obtained antibacterial effect was not concentration dependant. In the case of S. aureus, inhibition zone was narrower with the presence of modified bacteria in inhibition zone. Obtained antibacterial response pronounced dependence on concentration of silver within composite. According to results achieved with this investigation we concluded that HAp/Ag composite is effective against both, Gram positive and Gram negative bacteria and shows stronger activity against E. coli.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia",
title = "Antibacterial activity of hydroxyapatite/silver nanocomposite",
pages = "15-15",
url = "https://hdl.handle.net/21.15107/rcub_dais_3452"
}
Vukomanović, M., Repnik, U., Zavašnik, T., Škapin, S. D., Uskoković, D.,& Suvorov, D.. (2010). Antibacterial activity of hydroxyapatite/silver nanocomposite. in Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 15-15.
https://hdl.handle.net/21.15107/rcub_dais_3452
Vukomanović M, Repnik U, Zavašnik T, Škapin SD, Uskoković D, Suvorov D. Antibacterial activity of hydroxyapatite/silver nanocomposite. in Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia. 2010;:15-15.
https://hdl.handle.net/21.15107/rcub_dais_3452 .
Vukomanović, Marija, Repnik, U., Zavašnik, T., Škapin, Srečo Davor, Uskoković, Dragan, Suvorov, Danilo, "Antibacterial activity of hydroxyapatite/silver nanocomposite" in Program and the Book of Abstracts / Ninth Young Researchers Conference Materials Sciences and Engineering, December 20–22, 2010, Belgrade, Serbia (2010):15-15,
https://hdl.handle.net/21.15107/rcub_dais_3452 .