TY  - JOUR
AU  - Kuzminac, Ivana
AU  - Ćelić, Andjelka S.
AU  - Bekić, Sofija S.
AU  - Kojić, Vesna
AU  - Savić, Marina P.
AU  - Ignjatović, Nenad
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13031
AB  - Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.
PB  - Elsevier BV
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
SP  - 112597
VL  - 216
DO  - 10.1016/j.colsurfb.2022.112597
UR  - https://hdl.handle.net/21.15107/rcub_dais_13031
ER  - 

@article{
author = "Kuzminac, Ivana and Ćelić, Andjelka S. and Bekić, Sofija S. and Kojić, Vesna and Savić, Marina P. and Ignjatović, Nenad",
year = "2022",
abstract = "Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.",
publisher = "Elsevier BV",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells",
pages = "112597",
volume = "216",
doi = "10.1016/j.colsurfb.2022.112597",
url = "https://hdl.handle.net/21.15107/rcub_dais_13031"
}

Kuzminac, I., Ćelić, A. S., Bekić, S. S., Kojić, V., Savić, M. P.,& Ignjatović, N.. (2022). Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces
Elsevier BV., 216, 112597.
https://doi.org/10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13031

Kuzminac I, Ćelić AS, Bekić SS, Kojić V, Savić MP, Ignjatović N. Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces. 2022;216:112597.
doi:10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13031 .

Kuzminac, Ivana, Ćelić, Andjelka S., Bekić, Sofija S., Kojić, Vesna, Savić, Marina P., Ignjatović, Nenad, "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells" in Colloids and Surfaces B: Biointerfaces, 216 (2022):112597,
https://doi.org/10.1016/j.colsurfb.2022.112597 .,
https://hdl.handle.net/21.15107/rcub_dais_13031 .

TY  - JOUR
AU  - Kuzminac, Ivana
AU  - Ćelić, Andjelka S.
AU  - Bekić, Sofija S.
AU  - Kojić, Vesna
AU  - Savić, Marina P.
AU  - Ignjatović, Nenad
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13030
AB  - Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.
PB  - Elsevier BV
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
SP  - 112597
VL  - 216
DO  - 10.1016/j.colsurfb.2022.112597
UR  - https://hdl.handle.net/21.15107/rcub_dais_13030
ER  - 

@article{
author = "Kuzminac, Ivana and Ćelić, Andjelka S. and Bekić, Sofija S. and Kojić, Vesna and Savić, Marina P. and Ignjatović, Nenad",
year = "2022",
abstract = "Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.",
publisher = "Elsevier BV",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells",
pages = "112597",
volume = "216",
doi = "10.1016/j.colsurfb.2022.112597",
url = "https://hdl.handle.net/21.15107/rcub_dais_13030"
}

Kuzminac, I., Ćelić, A. S., Bekić, S. S., Kojić, V., Savić, M. P.,& Ignjatović, N.. (2022). Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces
Elsevier BV., 216, 112597.
https://doi.org/10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13030

Kuzminac I, Ćelić AS, Bekić SS, Kojić V, Savić MP, Ignjatović N. Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces. 2022;216:112597.
doi:10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13030 .

Kuzminac, Ivana, Ćelić, Andjelka S., Bekić, Sofija S., Kojić, Vesna, Savić, Marina P., Ignjatović, Nenad, "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells" in Colloids and Surfaces B: Biointerfaces, 216 (2022):112597,
https://doi.org/10.1016/j.colsurfb.2022.112597 .,
https://hdl.handle.net/21.15107/rcub_dais_13030 .