TY  - JOUR
AU  - Vučen, Sonja
AU  - Vuleta, Gordana
AU  - Crean, Abina
AU  - Moore, Anne C.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/383
AB  - Abstract

Objectives
The aim of our study was to evaluate the effect of designing ketoprofen-loaded nanosized spheres and combining them with solid silicon microneedles for enhanced and sustained percutaneous drug delivery.

Methods
Ketoprofen-loaded nanoparticles (KET-NP) were designed by modified solvent displacement method, using poly (D, L-lactic acid) (PDLLA). All prepared nanoparticles were characterised with regard to their particle size distribution, morphology, surface properties, thermal behaviour, drug content, drug release and stability. In-vitro skin permeation studies were conducted on Franz-type diffusion cells using porcine skin treated with ImmuPatch silicon microneedles (Tyndall Nation Institute, Cork, Ireland).

Key findings
The study showed that uniform nanospheres were prepared with high encapsulation efficiency and retained stable for 2 months. After an initial burst release, the PDLLA nanoparticles were capable of sustaining and controlling ketoprofen release that followed Korsmeyer–Peppas kinetics. An enhanced flux of ketoprofen was observed in the skin treated with silicon microneedles over a prolonged period of time.

Conclusions
Following application of silicon microneedle arrays, KET-NP were able to enhance ketoprofen flux and supply the porcine skin with drug over a prolonged (24 h) period of time. Our findings indicate that the delivery strategy described here could be used for the further development of effective and painless administration systems for sustained percutaneous delivery of ketoprofen.
PB  - Hoboken, NJ : John Wiley & Sons
T2  - Journal of Pharmacy and Pharmacology
T1  - Improved percutaneous delivery of ketoprofen using combined application of nanocarriers and silicon microneedles
SP  - 1451
EP  - 1462
VL  - 65
IS  - 10
DO  - 10.1111/jphp.12118
UR  - https://hdl.handle.net/21.15107/rcub_dais_383
ER  - 

@article{
author = "Vučen, Sonja and Vuleta, Gordana and Crean, Abina and Moore, Anne C. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2013",
abstract = "Abstract

Objectives
The aim of our study was to evaluate the effect of designing ketoprofen-loaded nanosized spheres and combining them with solid silicon microneedles for enhanced and sustained percutaneous drug delivery.

Methods
Ketoprofen-loaded nanoparticles (KET-NP) were designed by modified solvent displacement method, using poly (D, L-lactic acid) (PDLLA). All prepared nanoparticles were characterised with regard to their particle size distribution, morphology, surface properties, thermal behaviour, drug content, drug release and stability. In-vitro skin permeation studies were conducted on Franz-type diffusion cells using porcine skin treated with ImmuPatch silicon microneedles (Tyndall Nation Institute, Cork, Ireland).

Key findings
The study showed that uniform nanospheres were prepared with high encapsulation efficiency and retained stable for 2 months. After an initial burst release, the PDLLA nanoparticles were capable of sustaining and controlling ketoprofen release that followed Korsmeyer–Peppas kinetics. An enhanced flux of ketoprofen was observed in the skin treated with silicon microneedles over a prolonged period of time.

Conclusions
Following application of silicon microneedle arrays, KET-NP were able to enhance ketoprofen flux and supply the porcine skin with drug over a prolonged (24 h) period of time. Our findings indicate that the delivery strategy described here could be used for the further development of effective and painless administration systems for sustained percutaneous delivery of ketoprofen.",
publisher = "Hoboken, NJ : John Wiley & Sons",
journal = "Journal of Pharmacy and Pharmacology",
title = "Improved percutaneous delivery of ketoprofen using combined application of nanocarriers and silicon microneedles",
pages = "1451-1462",
volume = "65",
number = "10",
doi = "10.1111/jphp.12118",
url = "https://hdl.handle.net/21.15107/rcub_dais_383"
}

Vučen, S., Vuleta, G., Crean, A., Moore, A. C., Ignjatović, N.,& Uskoković, D.. (2013). Improved percutaneous delivery of ketoprofen using combined application of nanocarriers and silicon microneedles. in Journal of Pharmacy and Pharmacology
Hoboken, NJ : John Wiley & Sons., 65(10), 1451-1462.
https://doi.org/10.1111/jphp.12118
https://hdl.handle.net/21.15107/rcub_dais_383

Vučen S, Vuleta G, Crean A, Moore AC, Ignjatović N, Uskoković D. Improved percutaneous delivery of ketoprofen using combined application of nanocarriers and silicon microneedles. in Journal of Pharmacy and Pharmacology. 2013;65(10):1451-1462.
doi:10.1111/jphp.12118
https://hdl.handle.net/21.15107/rcub_dais_383 .

Vučen, Sonja, Vuleta, Gordana, Crean, Abina, Moore, Anne C., Ignjatović, Nenad, Uskoković, Dragan, "Improved percutaneous delivery of ketoprofen using combined application of nanocarriers and silicon microneedles" in Journal of Pharmacy and Pharmacology, 65, no. 10 (2013):1451-1462,
https://doi.org/10.1111/jphp.12118 .,
https://hdl.handle.net/21.15107/rcub_dais_383 .

TY  - CONF
AU  - Vučen, Sonja
AU  - Vuleta, Gordana
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2011
UR  - https://dais.sanu.ac.rs/123456789/182
AB  - Poster presented at the 3rd European Science Foundation Summer School Nanomedicine 2011, 19-24 June 2011
T1  - Comparative study of biodegradable polymers on the particle size, surface morphology and encapsulation efficiency of ketoprofen loaded nanoparticles
UR  - https://hdl.handle.net/21.15107/rcub_dais_182
ER  - 

@conference{
author = "Vučen, Sonja and Vuleta, Gordana and Ignjatović, Nenad and Uskoković, Dragan",
year = "2011",
abstract = "Poster presented at the 3rd European Science Foundation Summer School Nanomedicine 2011, 19-24 June 2011",
title = "Comparative study of biodegradable polymers on the particle size, surface morphology and encapsulation efficiency of ketoprofen loaded nanoparticles",
url = "https://hdl.handle.net/21.15107/rcub_dais_182"
}

Vučen, S., Vuleta, G., Ignjatović, N.,& Uskoković, D.. (2011). Comparative study of biodegradable polymers on the particle size, surface morphology and encapsulation efficiency of ketoprofen loaded nanoparticles. .
https://hdl.handle.net/21.15107/rcub_dais_182

Vučen S, Vuleta G, Ignjatović N, Uskoković D. Comparative study of biodegradable polymers on the particle size, surface morphology and encapsulation efficiency of ketoprofen loaded nanoparticles. 2011;.
https://hdl.handle.net/21.15107/rcub_dais_182 .

Vučen, Sonja, Vuleta, Gordana, Ignjatović, Nenad, Uskoković, Dragan, "Comparative study of biodegradable polymers on the particle size, surface morphology and encapsulation efficiency of ketoprofen loaded nanoparticles" (2011),
https://hdl.handle.net/21.15107/rcub_dais_182 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Vučen, Sonja
AU  - Vuleta, Gordana
AU  - Uskoković, Dragan
PY  - 2010
UR  - https://dais.sanu.ac.rs/123456789/3554
AB  - The transdermal delivery system provides a highly efficient, safe, and easy to use method for delivering active ingredients and other compounds to the body through intact skin, thereby offering many other advantages over the traditional dosage forms. Ketoprofen is a poorly watersoluble non-steroidal antiinflammatory drug and widely used for the acute and long-term treatment of rheumatoid arthritis and associated diseases. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for drug delivery of ketoprofene suitable for transdermal applications. Multi-scale design of a transdermal drug delivery system, from the molecular or nano scale through the macroscopic scale, was analyzed. The obtained nano particles contain ketoprofene-loaded spheres of poly(D,L-lactide acid) (PDLLA). Characterization was performed with wide-angle X-ray structural analysis (XRD), infrared spectroscopy (FT-IR), field-emission scanning electron microscopy (FESEM), high performance liquid chromatography (HPLC) and particle sized distribution (PSD) methods. XRD and FT-IR analyses confirmed that the ketoprofene is immobilized into PDLLA. The encapsulation of ketoprofen into the PDLLA nanoparticles was highly accomplished and the drug loading efficiency was 75%. The observed average particle dimension (d50) of ketoprofene-loaded nano particles was 63 nm.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and The Book of Abstracts / Twelfth Annual Conference YUCOMAT 2010, Herceg Novi, September 6–10, 2010
T1  - Nano-bioresorbable polymer spheres as carriers for transdermal delivery of ketoprofene
SP  - 61
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_dais_3554
ER  - 

@conference{
author = "Ignjatović, Nenad and Vučen, Sonja and Vuleta, Gordana and Uskoković, Dragan",
year = "2010",
abstract = "The transdermal delivery system provides a highly efficient, safe, and easy to use method for delivering active ingredients and other compounds to the body through intact skin, thereby offering many other advantages over the traditional dosage forms. Ketoprofen is a poorly watersoluble non-steroidal antiinflammatory drug and widely used for the acute and long-term treatment of rheumatoid arthritis and associated diseases. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for drug delivery of ketoprofene suitable for transdermal applications. Multi-scale design of a transdermal drug delivery system, from the molecular or nano scale through the macroscopic scale, was analyzed. The obtained nano particles contain ketoprofene-loaded spheres of poly(D,L-lactide acid) (PDLLA). Characterization was performed with wide-angle X-ray structural analysis (XRD), infrared spectroscopy (FT-IR), field-emission scanning electron microscopy (FESEM), high performance liquid chromatography (HPLC) and particle sized distribution (PSD) methods. XRD and FT-IR analyses confirmed that the ketoprofene is immobilized into PDLLA. The encapsulation of ketoprofen into the PDLLA nanoparticles was highly accomplished and the drug loading efficiency was 75%. The observed average particle dimension (d50) of ketoprofene-loaded nano particles was 63 nm.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and The Book of Abstracts / Twelfth Annual Conference YUCOMAT 2010, Herceg Novi, September 6–10, 2010",
title = "Nano-bioresorbable polymer spheres as carriers for transdermal delivery of ketoprofene",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_dais_3554"
}

Ignjatović, N., Vučen, S., Vuleta, G.,& Uskoković, D.. (2010). Nano-bioresorbable polymer spheres as carriers for transdermal delivery of ketoprofene. in Programme and The Book of Abstracts / Twelfth Annual Conference YUCOMAT 2010, Herceg Novi, September 6–10, 2010
Belgrade : Institute of Technical Sciences of SASA., 61-61.
https://hdl.handle.net/21.15107/rcub_dais_3554

Ignjatović N, Vučen S, Vuleta G, Uskoković D. Nano-bioresorbable polymer spheres as carriers for transdermal delivery of ketoprofene. in Programme and The Book of Abstracts / Twelfth Annual Conference YUCOMAT 2010, Herceg Novi, September 6–10, 2010. 2010;:61-61.
https://hdl.handle.net/21.15107/rcub_dais_3554 .

Ignjatović, Nenad, Vučen, Sonja, Vuleta, Gordana, Uskoković, Dragan, "Nano-bioresorbable polymer spheres as carriers for transdermal delivery of ketoprofene" in Programme and The Book of Abstracts / Twelfth Annual Conference YUCOMAT 2010, Herceg Novi, September 6–10, 2010 (2010):61-61,
https://hdl.handle.net/21.15107/rcub_dais_3554 .