TY  - JOUR
AU  - Kuzminac, Ivana
AU  - Ćelić, Andjelka S.
AU  - Bekić, Sofija S.
AU  - Kojić, Vesna
AU  - Savić, Marina P.
AU  - Ignjatović, Nenad
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13031
AB  - Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.
PB  - Elsevier BV
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
SP  - 112597
VL  - 216
DO  - 10.1016/j.colsurfb.2022.112597
UR  - https://hdl.handle.net/21.15107/rcub_dais_13031
ER  - 

@article{
author = "Kuzminac, Ivana and Ćelić, Andjelka S. and Bekić, Sofija S. and Kojić, Vesna and Savić, Marina P. and Ignjatović, Nenad",
year = "2022",
abstract = "Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.",
publisher = "Elsevier BV",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells",
pages = "112597",
volume = "216",
doi = "10.1016/j.colsurfb.2022.112597",
url = "https://hdl.handle.net/21.15107/rcub_dais_13031"
}

Kuzminac, I., Ćelić, A. S., Bekić, S. S., Kojić, V., Savić, M. P.,& Ignjatović, N.. (2022). Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces
Elsevier BV., 216, 112597.
https://doi.org/10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13031

Kuzminac I, Ćelić AS, Bekić SS, Kojić V, Savić MP, Ignjatović N. Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces. 2022;216:112597.
doi:10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13031 .

Kuzminac, Ivana, Ćelić, Andjelka S., Bekić, Sofija S., Kojić, Vesna, Savić, Marina P., Ignjatović, Nenad, "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells" in Colloids and Surfaces B: Biointerfaces, 216 (2022):112597,
https://doi.org/10.1016/j.colsurfb.2022.112597 .,
https://hdl.handle.net/21.15107/rcub_dais_13031 .

TY  - JOUR
AU  - Kuzminac, Ivana
AU  - Ćelić, Andjelka S.
AU  - Bekić, Sofija S.
AU  - Kojić, Vesna
AU  - Savić, Marina P.
AU  - Ignjatović, Nenad
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13030
AB  - Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.
PB  - Elsevier BV
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells
SP  - 112597
VL  - 216
DO  - 10.1016/j.colsurfb.2022.112597
UR  - https://hdl.handle.net/21.15107/rcub_dais_13030
ER  - 

@article{
author = "Kuzminac, Ivana and Ćelić, Andjelka S. and Bekić, Sofija S. and Kojić, Vesna and Savić, Marina P. and Ignjatović, Nenad",
year = "2022",
abstract = "Chemically modified steroids have a long history as anti-neoplastic drugs. Incorporation of a lactone moiety in the steroid nucleus, as in previously obtained 3β-acetoxy-17-oxa-17a-homoandrost-5-en-16-one (A) and 3β-hidroxy-17-oxa-17a-homoandrost-5-en-16-one (B), often results in enhanced anticancer properties. In this work, chitosan-based (Ch) nanoparticles were created and loaded with potent anticancer steroidal compounds, A and B. Changes to hormone receptor binding and cytotoxicity were then measured. In agreement with our previous results for A and B, A- and B-loaded Ch displayed cytotoxic properties against cancer cell lines. Both A-Ch and B-Ch showed activity toward estrogen negative breast cancer (MDA-MB-231) and androgen negative prostate cancer cell lines (PC-3). Greater selectivity toward cancer cells versus healthy lung fibroblast (MRC-5) was observed for B-Ch particles. Cell viability and cytotoxicity measurements after a recovery period indicate more robust recovery of healthy cells versus malignant cells. Compounds A and B or their Ch-encapsulated forms were shown to have negligible affinity for the ligand binding domain of estrogen receptor β or the androgen receptor in a fluorescent yeast screen, suggesting a lack of estrogenicity and androgenicity. Steroid-loaded chitosan nanoparticles display strong cytotoxicity towards MDA-MB-231 and PC-3 with a lack of hormone activity, indicating their safety and efficacy.",
publisher = "Elsevier BV",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells",
pages = "112597",
volume = "216",
doi = "10.1016/j.colsurfb.2022.112597",
url = "https://hdl.handle.net/21.15107/rcub_dais_13030"
}

Kuzminac, I., Ćelić, A. S., Bekić, S. S., Kojić, V., Savić, M. P.,& Ignjatović, N.. (2022). Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces
Elsevier BV., 216, 112597.
https://doi.org/10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13030

Kuzminac I, Ćelić AS, Bekić SS, Kojić V, Savić MP, Ignjatović N. Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells. in Colloids and Surfaces B: Biointerfaces. 2022;216:112597.
doi:10.1016/j.colsurfb.2022.112597
https://hdl.handle.net/21.15107/rcub_dais_13030 .

Kuzminac, Ivana, Ćelić, Andjelka S., Bekić, Sofija S., Kojić, Vesna, Savić, Marina P., Ignjatović, Nenad, "Hormone receptor binding, selectivity and cytotoxicity of steroid D-homo lactone loaded chitosan nanoparticles for the treatment of breast and prostate cancer cells" in Colloids and Surfaces B: Biointerfaces, 216 (2022):112597,
https://doi.org/10.1016/j.colsurfb.2022.112597 .,
https://hdl.handle.net/21.15107/rcub_dais_13030 .

TY  - JOUR
AU  - Veljović, Đorđe
AU  - Matić, Tamara
AU  - Stamenić, Tanja
AU  - Kojić, Vesna
AU  - Dimitrijević Branković, Suzana
AU  - Lukić, Miodrag J.
AU  - Jevtić, Sanja
AU  - Radovanović, Željko
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/S0272884219320413
UR  - https://dais.sanu.ac.rs/123456789/6948
AB  - The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.
PB  - Elsevier
T2  - Ceramics International
T1  - Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity
SP  - 22029
SP  - 22039
VL  - 45
IS  - 17, Part A
DO  - 10.1016/j.ceramint.2019.07.219
UR  - https://hdl.handle.net/21.15107/rcub_dais_6948
ER  - 

@article{
author = "Veljović, Đorđe and Matić, Tamara and Stamenić, Tanja and Kojić, Vesna and Dimitrijević Branković, Suzana and Lukić, Miodrag J. and Jevtić, Sanja and Radovanović, Željko and Petrović, Rada and Janaćković, Đorđe",
year = "2019",
abstract = "The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.",
publisher = "Elsevier",
journal = "Ceramics International",
title = "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity",
pages = "22029-22039",
volume = "45",
number = "17, Part A",
doi = "10.1016/j.ceramint.2019.07.219",
url = "https://hdl.handle.net/21.15107/rcub_dais_6948"
}

Veljović, Đ., Matić, T., Stamenić, T., Kojić, V., Dimitrijević Branković, S., Lukić, M. J., Jevtić, S., Radovanović, Ž., Petrović, R.,& Janaćković, Đ.. (2019). Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International
Elsevier., 45(17, Part A), 22029.
https://doi.org/10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6948

Veljović Đ, Matić T, Stamenić T, Kojić V, Dimitrijević Branković S, Lukić MJ, Jevtić S, Radovanović Ž, Petrović R, Janaćković Đ. Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International. 2019;45(17, Part A):22029.
doi:10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6948 .

Veljović, Đorđe, Matić, Tamara, Stamenić, Tanja, Kojić, Vesna, Dimitrijević Branković, Suzana, Lukić, Miodrag J., Jevtić, Sanja, Radovanović, Željko, Petrović, Rada, Janaćković, Đorđe, "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity" in Ceramics International, 45, no. 17, Part A (2019):22029,
https://doi.org/10.1016/j.ceramint.2019.07.219 .,
https://hdl.handle.net/21.15107/rcub_dais_6948 .

TY  - JOUR
AU  - Veljović, Đorđe
AU  - Matić, Tamara
AU  - Stamenić, Tanja
AU  - Kojić, Vesna
AU  - Dimitrijević Branković, Suzana
AU  - Lukić, Miodrag J.
AU  - Jevtić, Sanja
AU  - Radovanović, Željko
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/S0272884219320413
UR  - https://dais.sanu.ac.rs/123456789/6504
AB  - The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.
PB  - Elsevier
T2  - Ceramics International
T1  - Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity
SP  - 22029
SP  - 22039
VL  - 45
IS  - 17, Part A
DO  - 10.1016/j.ceramint.2019.07.219
UR  - https://hdl.handle.net/21.15107/rcub_dais_6504
ER  - 

@article{
author = "Veljović, Đorđe and Matić, Tamara and Stamenić, Tanja and Kojić, Vesna and Dimitrijević Branković, Suzana and Lukić, Miodrag J. and Jevtić, Sanja and Radovanović, Željko and Petrović, Rada and Janaćković, Đorđe",
year = "2019",
abstract = "The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.",
publisher = "Elsevier",
journal = "Ceramics International",
title = "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity",
pages = "22029-22039",
volume = "45",
number = "17, Part A",
doi = "10.1016/j.ceramint.2019.07.219",
url = "https://hdl.handle.net/21.15107/rcub_dais_6504"
}

Veljović, Đ., Matić, T., Stamenić, T., Kojić, V., Dimitrijević Branković, S., Lukić, M. J., Jevtić, S., Radovanović, Ž., Petrović, R.,& Janaćković, Đ.. (2019). Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International
Elsevier., 45(17, Part A), 22029.
https://doi.org/10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6504

Veljović Đ, Matić T, Stamenić T, Kojić V, Dimitrijević Branković S, Lukić MJ, Jevtić S, Radovanović Ž, Petrović R, Janaćković Đ. Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International. 2019;45(17, Part A):22029.
doi:10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6504 .

Veljović, Đorđe, Matić, Tamara, Stamenić, Tanja, Kojić, Vesna, Dimitrijević Branković, Suzana, Lukić, Miodrag J., Jevtić, Sanja, Radovanović, Željko, Petrović, Rada, Janaćković, Đorđe, "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity" in Ceramics International, 45, no. 17, Part A (2019):22029,
https://doi.org/10.1016/j.ceramint.2019.07.219 .,
https://hdl.handle.net/21.15107/rcub_dais_6504 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Penov Gaši, Katarina
AU  - Ajduković, Jovana
AU  - Kojić, Vesna
AU  - Marković, Smilja
AU  - Uskoković, Dragan
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4067
AB  - An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells.
PB  - Elsevier
T2  - Materials Science and Engineering: C
T1  - The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites
SP  - 371
EP  - 377
VL  - 89
IS  - 1
DO  - 10.1016/j.msec.2018.04.028
UR  - https://hdl.handle.net/21.15107/rcub_dais_4067
ER  - 

@article{
author = "Ignjatović, Nenad and Penov Gaši, Katarina and Ajduković, Jovana and Kojić, Vesna and Marković, Smilja and Uskoković, Dragan",
year = "2018",
abstract = "An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells.",
publisher = "Elsevier",
journal = "Materials Science and Engineering: C",
title = "The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites",
pages = "371-377",
volume = "89",
number = "1",
doi = "10.1016/j.msec.2018.04.028",
url = "https://hdl.handle.net/21.15107/rcub_dais_4067"
}

Ignjatović, N., Penov Gaši, K., Ajduković, J., Kojić, V., Marković, S.,& Uskoković, D.. (2018). The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites. in Materials Science and Engineering: C
Elsevier., 89(1), 371-377.
https://doi.org/10.1016/j.msec.2018.04.028
https://hdl.handle.net/21.15107/rcub_dais_4067

Ignjatović N, Penov Gaši K, Ajduković J, Kojić V, Marković S, Uskoković D. The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites. in Materials Science and Engineering: C. 2018;89(1):371-377.
doi:10.1016/j.msec.2018.04.028
https://hdl.handle.net/21.15107/rcub_dais_4067 .

Ignjatović, Nenad, Penov Gaši, Katarina, Ajduković, Jovana, Kojić, Vesna, Marković, Smilja, Uskoković, Dragan, "The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites" in Materials Science and Engineering: C, 89, no. 1 (2018):371-377,
https://doi.org/10.1016/j.msec.2018.04.028 .,
https://hdl.handle.net/21.15107/rcub_dais_4067 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Penov Gaši, Katarina
AU  - Ajduković, Jovana
AU  - Kojić, Vesna V.
AU  - Marković, Smilja
AU  - Uskoković, Dragan
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/3699
AB  - An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites
SP  - 371
EP  - 377
VL  - 89
DO  - 10.1016/j.msec.2018.04.028
UR  - https://hdl.handle.net/21.15107/rcub_dais_3699
ER  - 

@article{
author = "Ignjatović, Nenad and Penov Gaši, Katarina and Ajduković, Jovana and Kojić, Vesna V. and Marković, Smilja and Uskoković, Dragan",
year = "2018",
abstract = "An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites",
pages = "371-377",
volume = "89",
doi = "10.1016/j.msec.2018.04.028",
url = "https://hdl.handle.net/21.15107/rcub_dais_3699"
}

Ignjatović, N., Penov Gaši, K., Ajduković, J., Kojić, V. V., Marković, S.,& Uskoković, D.. (2018). The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites. in Materials Science and Engineering C
Elsevier., 89, 371-377.
https://doi.org/10.1016/j.msec.2018.04.028
https://hdl.handle.net/21.15107/rcub_dais_3699

Ignjatović N, Penov Gaši K, Ajduković J, Kojić VV, Marković S, Uskoković D. The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites. in Materials Science and Engineering C. 2018;89:371-377.
doi:10.1016/j.msec.2018.04.028
https://hdl.handle.net/21.15107/rcub_dais_3699 .

Ignjatović, Nenad, Penov Gaši, Katarina, Ajduković, Jovana, Kojić, Vesna V., Marković, Smilja, Uskoković, Dragan, "The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites" in Materials Science and Engineering C, 89 (2018):371-377,
https://doi.org/10.1016/j.msec.2018.04.028 .,
https://hdl.handle.net/21.15107/rcub_dais_3699 .

TY  - CONF
AU  - Veljović, Đorđe
AU  - Radovanović, Željko
AU  - Rogan, Jelena
AU  - Dapčević, Aleksandra
AU  - Dimitrijević, Suzana
AU  - Dimitrijević Branković, Suzana
AU  - Kojić, Vesna
AU  - Janaćković, Đorđe
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4718
AB  - Bioactive glasses have been used for over three decades in biomedical applications owing to high bioactivity, biocompatibility, as well as the possibility to stimulate regeneration of the bone. The aim of this work was to synthesized bioactive glasses, which contain lithium and strontium, by commercial method melting-quenching, as well as determining the properties, affected by mentioned ions. Differential thermal/thermogravimetric analysis, particle size distribution, energy-dispersive X-ray spectroscopy, inductively coupled plasma optical emission spectrometry, evaluation of the antimicrobial activity, in vitro bioactivity and biocompatibility test and scanning electron microscopy were used for characterization. The results showed that glass transition and crystallization temperatures are decreasing with addition of lithium and strontium ions. Furthermore, the samples showed high inhibition rate of Escherichia coli growth, as well as high bioactivity and biocompatibility. The crystal apatite layer, formed on the surface of synthesized glasses after seven days in simulated body fluid, differs in shape, size and interconnection of the glass particles, which depends on concentration of lithium and strontium ions.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
T1  - Synthesis and characterization of bioactive glass doped with lithium and strontium ions
SP  - 20
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_dais_4718
ER  - 

@conference{
author = "Veljović, Đorđe and Radovanović, Željko and Rogan, Jelena and Dapčević, Aleksandra and Dimitrijević, Suzana and Dimitrijević Branković, Suzana and Kojić, Vesna and Janaćković, Đorđe",
year = "2018",
abstract = "Bioactive glasses have been used for over three decades in biomedical applications owing to high bioactivity, biocompatibility, as well as the possibility to stimulate regeneration of the bone. The aim of this work was to synthesized bioactive glasses, which contain lithium and strontium, by commercial method melting-quenching, as well as determining the properties, affected by mentioned ions. Differential thermal/thermogravimetric analysis, particle size distribution, energy-dispersive X-ray spectroscopy, inductively coupled plasma optical emission spectrometry, evaluation of the antimicrobial activity, in vitro bioactivity and biocompatibility test and scanning electron microscopy were used for characterization. The results showed that glass transition and crystallization temperatures are decreasing with addition of lithium and strontium ions. Furthermore, the samples showed high inhibition rate of Escherichia coli growth, as well as high bioactivity and biocompatibility. The crystal apatite layer, formed on the surface of synthesized glasses after seven days in simulated body fluid, differs in shape, size and interconnection of the glass particles, which depends on concentration of lithium and strontium ions.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia",
title = "Synthesis and characterization of bioactive glass doped with lithium and strontium ions",
pages = "20-20",
url = "https://hdl.handle.net/21.15107/rcub_dais_4718"
}

Veljović, Đ., Radovanović, Ž., Rogan, J., Dapčević, A., Dimitrijević, S., Dimitrijević Branković, S., Kojić, V.,& Janaćković, Đ.. (2018). Synthesis and characterization of bioactive glass doped with lithium and strontium ions. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 20-20.
https://hdl.handle.net/21.15107/rcub_dais_4718

Veljović Đ, Radovanović Ž, Rogan J, Dapčević A, Dimitrijević S, Dimitrijević Branković S, Kojić V, Janaćković Đ. Synthesis and characterization of bioactive glass doped with lithium and strontium ions. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia. 2018;:20-20.
https://hdl.handle.net/21.15107/rcub_dais_4718 .

Veljović, Đorđe, Radovanović, Željko, Rogan, Jelena, Dapčević, Aleksandra, Dimitrijević, Suzana, Dimitrijević Branković, Suzana, Kojić, Vesna, Janaćković, Đorđe, "Synthesis and characterization of bioactive glass doped with lithium and strontium ions" in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia (2018):20-20,
https://hdl.handle.net/21.15107/rcub_dais_4718 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Sakač, Marija
AU  - Kuzminac, Ivana
AU  - Kojić, Vesna
AU  - Marković, Smilja
AU  - Vasiljević Radović, Dana
AU  - Wu, Victoria M.
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2018
UR  - https://pubs.rsc.org/en/content/articlelanding/2018/tb/c8tb01995a
UR  - https://dais.sanu.ac.rs/123456789/4509
AB  - Low targeting efficiency and fast metabolism of antineoplastic drugs are hindrances to effective chemotherapies and there is an ongoing search for better drugs, but also better carriers. Steroid derivatives, 3β-hydroxy-16-hydroxymino-androst-5-en-17-one (A) and 3β,17β-dihydroxy-16-hydroxymino-androst-5-ene (B) as cancer growth inhibitors were chemically synthesized and captured in a carrier composed of hydroxyapatite (HAp) nanoparticles coated with chitosan oligosaccharide lactate (ChOLS). The only difference between the two derivatives is that A has a carbonyl group at the C17 position of the five-membered ring and B has a hydroxyl. This small difference in the structure resulted not only in different physicochemical properties of the A- and B-loaded HAp/ChOSL, but also in different biological activities. The morphology of drug-loaded HAp/ChOSL particles was spherical, but the size depended on the drug identity: d50 = 138 nm for A-loaded HAp/ChOSL and d50 = 223 nm for B-loaded HAp/ChOSL. Cell-selective toxicity was tested against human breast carcinoma (MCF7 and MDA-MB-231), human lung carcinoma (A549) and human lung fibroblasts (MRC-5). The small selectivity of pure derivatives A and B toward breast cancer cells became drastically increased when they were delivered using HAp/ChOSL particles. Whereas the ratio of the cytotoxicity imposed onto breast cancer cells and the cytotoxicity imposed onto healthy MRC-5 fibroblasts ranged from 1.5 to 1.7 for pure A and from 1.5 to 2.3 for pure derivative B depending on the concentration, it increased to 5.4 for A-loaded HAp/ChOSL and 5.1 for B-loaded HAp/ChOSL. FACS analysis demonstrated poor uptake of HAp/ChOSL particles by MCF7 cells, suggesting that the drug release occurs extracellularly. The augmented activity of the drugs was most likely due to sustained release, although the favorable positive charge of the carrier, allowing it to adhere to the negatively charged plasma membrane and release the drugs steadily and directly to the hydrophobic cell membrane milieu, was delineated as a possible complementary mechanism.
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells
SP  - 6957
EP  - 696
VL  - 6
DO  - 10.1039/C8TB01995A
UR  - https://hdl.handle.net/21.15107/rcub_dais_4509
ER  - 

@article{
author = "Ignjatović, Nenad and Sakač, Marija and Kuzminac, Ivana and Kojić, Vesna and Marković, Smilja and Vasiljević Radović, Dana and Wu, Victoria M. and Uskoković, Vuk and Uskoković, Dragan",
year = "2018",
abstract = "Low targeting efficiency and fast metabolism of antineoplastic drugs are hindrances to effective chemotherapies and there is an ongoing search for better drugs, but also better carriers. Steroid derivatives, 3β-hydroxy-16-hydroxymino-androst-5-en-17-one (A) and 3β,17β-dihydroxy-16-hydroxymino-androst-5-ene (B) as cancer growth inhibitors were chemically synthesized and captured in a carrier composed of hydroxyapatite (HAp) nanoparticles coated with chitosan oligosaccharide lactate (ChOLS). The only difference between the two derivatives is that A has a carbonyl group at the C17 position of the five-membered ring and B has a hydroxyl. This small difference in the structure resulted not only in different physicochemical properties of the A- and B-loaded HAp/ChOSL, but also in different biological activities. The morphology of drug-loaded HAp/ChOSL particles was spherical, but the size depended on the drug identity: d50 = 138 nm for A-loaded HAp/ChOSL and d50 = 223 nm for B-loaded HAp/ChOSL. Cell-selective toxicity was tested against human breast carcinoma (MCF7 and MDA-MB-231), human lung carcinoma (A549) and human lung fibroblasts (MRC-5). The small selectivity of pure derivatives A and B toward breast cancer cells became drastically increased when they were delivered using HAp/ChOSL particles. Whereas the ratio of the cytotoxicity imposed onto breast cancer cells and the cytotoxicity imposed onto healthy MRC-5 fibroblasts ranged from 1.5 to 1.7 for pure A and from 1.5 to 2.3 for pure derivative B depending on the concentration, it increased to 5.4 for A-loaded HAp/ChOSL and 5.1 for B-loaded HAp/ChOSL. FACS analysis demonstrated poor uptake of HAp/ChOSL particles by MCF7 cells, suggesting that the drug release occurs extracellularly. The augmented activity of the drugs was most likely due to sustained release, although the favorable positive charge of the carrier, allowing it to adhere to the negatively charged plasma membrane and release the drugs steadily and directly to the hydrophobic cell membrane milieu, was delineated as a possible complementary mechanism.",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells",
pages = "6957-696",
volume = "6",
doi = "10.1039/C8TB01995A",
url = "https://hdl.handle.net/21.15107/rcub_dais_4509"
}

Ignjatović, N., Sakač, M., Kuzminac, I., Kojić, V., Marković, S., Vasiljević Radović, D., Wu, V. M., Uskoković, V.,& Uskoković, D.. (2018). Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells. in Journal of Materials Chemistry B
Royal Society of Chemistry., 6, 6957-696.
https://doi.org/10.1039/C8TB01995A
https://hdl.handle.net/21.15107/rcub_dais_4509

Ignjatović N, Sakač M, Kuzminac I, Kojić V, Marković S, Vasiljević Radović D, Wu VM, Uskoković V, Uskoković D. Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells. in Journal of Materials Chemistry B. 2018;6:6957-696.
doi:10.1039/C8TB01995A
https://hdl.handle.net/21.15107/rcub_dais_4509 .

Ignjatović, Nenad, Sakač, Marija, Kuzminac, Ivana, Kojić, Vesna, Marković, Smilja, Vasiljević Radović, Dana, Wu, Victoria M., Uskoković, Vuk, Uskoković, Dragan, "Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells" in Journal of Materials Chemistry B, 6 (2018):6957-696,
https://doi.org/10.1039/C8TB01995A .,
https://hdl.handle.net/21.15107/rcub_dais_4509 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Sakač, Marija
AU  - Kuzminac, Ivana
AU  - Kojić, Vesna
AU  - Marković, Smilja
AU  - Vasiljević Radović, Dana
AU  - Wu, Victoria
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4066
AB  - Low targeting efficiency and fast metabolism of antineoplastic drugs are hindrances to effective chemotherapies and there is an ongoing search for better drugs, but also better carriers. Steroid derivatives, 3β-hydroxy-16-hydroxymino-androst-5-en-17-one (A) and 3β,17β-dihydroxy-16-hydroxymino-androst-5-ene (B) as cancer growth inhibitors were chemically synthesized and captured in a carrier composed of hydroxyapatite (HAp) nanoparticles coated with chitosan oligosaccharide lactate (ChOLS). The only difference between the two derivatives is that A has a carbonyl group at the C17 position of the five-membered ring and B has a hydroxyl. This small difference in the structure resulted not only in different physicochemical properties of the A- and B-loaded HAp/ChOSL, but also in different biological activities. The morphology of drug-loaded HAp/ChOSL particles was spherical, but the size depended on the drug identity: d50 = 138 nm for A-loaded HAp/ChOSL and d50 = 223 nm for B-loaded HAp/ChOSL. Cell-selective toxicity was tested against human breast carcinoma (MCF7 and MDA-MB-231), human lung carcinoma (A549) and human lung fibroblasts (MRC-5). The small selectivity of pure derivatives A and B toward breast cancer cells became drastically increased when they were delivered using HAp/ChOSL particles. Whereas the ratio of the cytotoxicity imposed onto breast cancer cells and the cytotoxicity imposed onto healthy MRC-5 fibroblasts ranged from 1.5 to 1.7 for pure A and from 1.5 to 2.3 for pure derivative B depending on the concentration, it increased to 5.4 for A-loaded HAp/ChOSL and 5.1 for B-loaded HAp/ChOSL. FACS analysis demonstrated poor uptake of HAp/ChOSL particles by MCF7 cells, suggesting that the drug release occurs extracellularly. The augmented activity of the drugs was most likely due to sustained release, although the favorable positive charge of the carrier, allowing it to adhere to the negatively charged plasma membrane and release the drugs steadily and directly to the hydrophobic cell membrane milieu, was delineated as a possible complementary mechanism.
PB  - Royal Society of Chemistry
T2  - Journal of Materials Chemistry B
T1  - Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells
SP  - 6957
EP  - 6968
VL  - 6
DO  - 10.1039/C8TB01995A
UR  - https://hdl.handle.net/21.15107/rcub_dais_4066
ER  - 

@article{
author = "Ignjatović, Nenad and Sakač, Marija and Kuzminac, Ivana and Kojić, Vesna and Marković, Smilja and Vasiljević Radović, Dana and Wu, Victoria and Uskoković, Vuk and Uskoković, Dragan",
year = "2018",
abstract = "Low targeting efficiency and fast metabolism of antineoplastic drugs are hindrances to effective chemotherapies and there is an ongoing search for better drugs, but also better carriers. Steroid derivatives, 3β-hydroxy-16-hydroxymino-androst-5-en-17-one (A) and 3β,17β-dihydroxy-16-hydroxymino-androst-5-ene (B) as cancer growth inhibitors were chemically synthesized and captured in a carrier composed of hydroxyapatite (HAp) nanoparticles coated with chitosan oligosaccharide lactate (ChOLS). The only difference between the two derivatives is that A has a carbonyl group at the C17 position of the five-membered ring and B has a hydroxyl. This small difference in the structure resulted not only in different physicochemical properties of the A- and B-loaded HAp/ChOSL, but also in different biological activities. The morphology of drug-loaded HAp/ChOSL particles was spherical, but the size depended on the drug identity: d50 = 138 nm for A-loaded HAp/ChOSL and d50 = 223 nm for B-loaded HAp/ChOSL. Cell-selective toxicity was tested against human breast carcinoma (MCF7 and MDA-MB-231), human lung carcinoma (A549) and human lung fibroblasts (MRC-5). The small selectivity of pure derivatives A and B toward breast cancer cells became drastically increased when they were delivered using HAp/ChOSL particles. Whereas the ratio of the cytotoxicity imposed onto breast cancer cells and the cytotoxicity imposed onto healthy MRC-5 fibroblasts ranged from 1.5 to 1.7 for pure A and from 1.5 to 2.3 for pure derivative B depending on the concentration, it increased to 5.4 for A-loaded HAp/ChOSL and 5.1 for B-loaded HAp/ChOSL. FACS analysis demonstrated poor uptake of HAp/ChOSL particles by MCF7 cells, suggesting that the drug release occurs extracellularly. The augmented activity of the drugs was most likely due to sustained release, although the favorable positive charge of the carrier, allowing it to adhere to the negatively charged plasma membrane and release the drugs steadily and directly to the hydrophobic cell membrane milieu, was delineated as a possible complementary mechanism.",
publisher = "Royal Society of Chemistry",
journal = "Journal of Materials Chemistry B",
title = "Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells",
pages = "6957-6968",
volume = "6",
doi = "10.1039/C8TB01995A",
url = "https://hdl.handle.net/21.15107/rcub_dais_4066"
}

Ignjatović, N., Sakač, M., Kuzminac, I., Kojić, V., Marković, S., Vasiljević Radović, D., Wu, V., Uskoković, V.,& Uskoković, D.. (2018). Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells. in Journal of Materials Chemistry B
Royal Society of Chemistry., 6, 6957-6968.
https://doi.org/10.1039/C8TB01995A
https://hdl.handle.net/21.15107/rcub_dais_4066

Ignjatović N, Sakač M, Kuzminac I, Kojić V, Marković S, Vasiljević Radović D, Wu V, Uskoković V, Uskoković D. Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells. in Journal of Materials Chemistry B. 2018;6:6957-6968.
doi:10.1039/C8TB01995A
https://hdl.handle.net/21.15107/rcub_dais_4066 .

Ignjatović, Nenad, Sakač, Marija, Kuzminac, Ivana, Kojić, Vesna, Marković, Smilja, Vasiljević Radović, Dana, Wu, Victoria, Uskoković, Vuk, Uskoković, Dragan, "Chitosan oligosaccharide lactate coated hydroxyapatite nanoparticles as a vehicle for the delivery of steroid drugs and the targeting of breast cancer cells" in Journal of Materials Chemistry B, 6 (2018):6957-6968,
https://doi.org/10.1039/C8TB01995A .,
https://hdl.handle.net/21.15107/rcub_dais_4066 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Sakač, Marija
AU  - Kuzminac, Ivana
AU  - Kojić, Vesna V.
AU  - Marković, Smilja
AU  - Wu, Victoria
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/3662
AB  - The applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Hybrid systems based on nano hydroxyapatites (HAp) are the subject of numerous studies in preventive and regenerative medicine. HAp nanoparticles coated with bioresorbable polymers have been successfully used as fillers, carriers of antibiotics, vitamins and stem cells in bone tissue engineering, etc. In this study we utilize an emulsification process and freeze drying to load the hybrid system made of nano HAp particles coated with chitosan oligosaccharide lactate (ChOSL) with two different but similar steroid derivatives: 3β-hydroxy- 16-hydroxymino-androst-5-ene-17-one (A), C19H27NO3 and 3β, 17β-dihydroxy-16-hydroxyminoandrost- 5-ene (B), C19H29NO3. The cell-selective toxicity of HAp particles coated with of A- or B-loaded ChOSL was examined simultaneously on the following cell lines: human breast carcinoma (MCF-7, MDA-MB-231), human lung carcinoma (A549) and human lung fibroblasts (MRC-5), using dye exclusion (DET) and MTT assays. 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivatives A or B. FT-IR, XRD, DTA, TGA and DSC techniques confirmed the drug loading process of steroide (A or B) in core–shell particles based on nano hydroxyapatite. Atomic force microscopy and particle size analyses were used to confirm that the particles were spherical with sizes between 80 and 240 nm. The measured values of electrokinetic parameters (zeta potential, electrophoretic mobility and conductivity) were significantly different for the steroid free carrier (HAp/ChOLS) and A- or B-loaded ChOSL. The value of the topological molecular polar surface area (TPSA, the sum of the surfaces of polar atoms and groups in the molecule), were also different for drug free carrier and A- or BHAp/ ChOLS. Highly selective anticancer activity was noted towards breast cancer cells (MDAMB- 231) by B-loaded HAp/ChOLS. DET testing after 48 hours (after incubation and recovery) of the treatment with A-HAp/ChOSL and B-HAp/ChOSL particles showed a high viability of healthy cells (over 80%). The lowest viability was found in MDA-MB-231 cancer cells treated with B-HAp/ChOSL (28%). The obtained results of the DET and MTT tests showed that the particles of A-HAp/ChOLS exhibited nearly four-fold greater cytotoxicity towards breast cancer cells (MDA-MB-231) than towards healthy cells (MRC-5). B-HAp/ChOSL particles exhibited nearly six times greater cytotoxicity to all breast cancer cells than to healthy ones.
PB  - Belgrade : Materials Research Society of Serbia
C3  - Programme and The Book of Abstracts / Twentieth Annual Conference YUCOMAT 2018, Herceg Novi, September 3-7, 2018
T1  - Cell-selective toxicity of hydroxyapatite-chitosan oligosaccharide lactate particles loaded with a steroid cancer inhibitor
SP  - 74
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_dais_3662
ER  - 

@conference{
author = "Ignjatović, Nenad and Sakač, Marija and Kuzminac, Ivana and Kojić, Vesna V. and Marković, Smilja and Wu, Victoria and Uskoković, Vuk and Uskoković, Dragan",
year = "2018",
abstract = "The applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Hybrid systems based on nano hydroxyapatites (HAp) are the subject of numerous studies in preventive and regenerative medicine. HAp nanoparticles coated with bioresorbable polymers have been successfully used as fillers, carriers of antibiotics, vitamins and stem cells in bone tissue engineering, etc. In this study we utilize an emulsification process and freeze drying to load the hybrid system made of nano HAp particles coated with chitosan oligosaccharide lactate (ChOSL) with two different but similar steroid derivatives: 3β-hydroxy- 16-hydroxymino-androst-5-ene-17-one (A), C19H27NO3 and 3β, 17β-dihydroxy-16-hydroxyminoandrost- 5-ene (B), C19H29NO3. The cell-selective toxicity of HAp particles coated with of A- or B-loaded ChOSL was examined simultaneously on the following cell lines: human breast carcinoma (MCF-7, MDA-MB-231), human lung carcinoma (A549) and human lung fibroblasts (MRC-5), using dye exclusion (DET) and MTT assays. 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivatives A or B. FT-IR, XRD, DTA, TGA and DSC techniques confirmed the drug loading process of steroide (A or B) in core–shell particles based on nano hydroxyapatite. Atomic force microscopy and particle size analyses were used to confirm that the particles were spherical with sizes between 80 and 240 nm. The measured values of electrokinetic parameters (zeta potential, electrophoretic mobility and conductivity) were significantly different for the steroid free carrier (HAp/ChOLS) and A- or B-loaded ChOSL. The value of the topological molecular polar surface area (TPSA, the sum of the surfaces of polar atoms and groups in the molecule), were also different for drug free carrier and A- or BHAp/ ChOLS. Highly selective anticancer activity was noted towards breast cancer cells (MDAMB- 231) by B-loaded HAp/ChOLS. DET testing after 48 hours (after incubation and recovery) of the treatment with A-HAp/ChOSL and B-HAp/ChOSL particles showed a high viability of healthy cells (over 80%). The lowest viability was found in MDA-MB-231 cancer cells treated with B-HAp/ChOSL (28%). The obtained results of the DET and MTT tests showed that the particles of A-HAp/ChOLS exhibited nearly four-fold greater cytotoxicity towards breast cancer cells (MDA-MB-231) than towards healthy cells (MRC-5). B-HAp/ChOSL particles exhibited nearly six times greater cytotoxicity to all breast cancer cells than to healthy ones.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "Programme and The Book of Abstracts / Twentieth Annual Conference YUCOMAT 2018, Herceg Novi, September 3-7, 2018",
title = "Cell-selective toxicity of hydroxyapatite-chitosan oligosaccharide lactate particles loaded with a steroid cancer inhibitor",
pages = "74-75",
url = "https://hdl.handle.net/21.15107/rcub_dais_3662"
}

Ignjatović, N., Sakač, M., Kuzminac, I., Kojić, V. V., Marković, S., Wu, V., Uskoković, V.,& Uskoković, D.. (2018). Cell-selective toxicity of hydroxyapatite-chitosan oligosaccharide lactate particles loaded with a steroid cancer inhibitor. in Programme and The Book of Abstracts / Twentieth Annual Conference YUCOMAT 2018, Herceg Novi, September 3-7, 2018
Belgrade : Materials Research Society of Serbia., 74-75.
https://hdl.handle.net/21.15107/rcub_dais_3662

Ignjatović N, Sakač M, Kuzminac I, Kojić VV, Marković S, Wu V, Uskoković V, Uskoković D. Cell-selective toxicity of hydroxyapatite-chitosan oligosaccharide lactate particles loaded with a steroid cancer inhibitor. in Programme and The Book of Abstracts / Twentieth Annual Conference YUCOMAT 2018, Herceg Novi, September 3-7, 2018. 2018;:74-75.
https://hdl.handle.net/21.15107/rcub_dais_3662 .

Ignjatović, Nenad, Sakač, Marija, Kuzminac, Ivana, Kojić, Vesna V., Marković, Smilja, Wu, Victoria, Uskoković, Vuk, Uskoković, Dragan, "Cell-selective toxicity of hydroxyapatite-chitosan oligosaccharide lactate particles loaded with a steroid cancer inhibitor" in Programme and The Book of Abstracts / Twentieth Annual Conference YUCOMAT 2018, Herceg Novi, September 3-7, 2018 (2018):74-75,
https://hdl.handle.net/21.15107/rcub_dais_3662 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Penov Gaši, Katarina
AU  - Ajduković, Jovana
AU  - Sakač, Marija
AU  - Kuzminac, Ivana
AU  - Kojić, Vesna V.
AU  - Marković, Smilja
AU  - Uskoković, Dragan
PY  - 2017
UR  - https://dais.sanu.ac.rs/123456789/15442
AB  - Hybrid systems based on nano hydroxyapatites (HAp) are the subject of numerous studies in preventive and regenerative medicine. Special interests are directed towards the creation of a system based on HAp for use in a nano-oncology. The main objective of this research is directed towards the creation of a system with cytotoxic properties towards the cancer cells with the same time, minimum side effects. Carriers base on core shell of HAp/chitosan-poly(D,L)-lactide-coglycolide (PLGA) loaded with androstane-based cancer inhibitor could be seen as promising drug delivery platforms for selective cancer therapies.
In this study we utilize an emulsification process and freeze drying to load the composite particles based on HAp nanocarrier, chitosane (Ch), PLGA and chitosan oligosaccharide lactate (ChOL) with 17β-hydroxy-17α-picolyl-androst-5-en-3β-acetate (A) and 3β,17β-dihydroxy-16-hydroxymino-androst-5-en (B), a chemotherapeutic derivatives of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormone-dependent cancers (lung, prostate and colon cancer; adeno and cervix carcinoma; etc.).
1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivatives A and B. The thermogravimetric and differential thermal analysis (TGA, DTA) coupled with mass spectrometry was used to qualitatively confirm the drug loading process. FT-IR, XRD, AFM and DSC techniques have confirmed the success of androstane (A and B) loading process in core shell particles base on nano hydroxyapatite. All the synthesized particles were found to be spherical in shape with a uniform size distribution from d50=167 to d50=231 nm. Highly selective anticancer activity was noted towards the human lung carcinoma (A549) by A loaded HAp/Ch-PLGA and towards the human breast adenocarcinoma (MDA-MB-231) by B loaded HAp/ChOL. The obtained results of the DET and MTT tests were in agreement.
PB  - Belgrade : Materials Research Society of Serbia
C3  - Programme and The Book of Abstracts / Nineteenth Annual Conference YUCOMAT 2017, Herceg Novi, September 4-8, 2017
T1  - Highly selective anticancer activity of core shell particles based on hydroxyapatite, chitosan lactate and different androstane derivatives
SP  - 50
EP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_dais_15442
ER  - 

@conference{
author = "Ignjatović, Nenad and Penov Gaši, Katarina and Ajduković, Jovana and Sakač, Marija and Kuzminac, Ivana and Kojić, Vesna V. and Marković, Smilja and Uskoković, Dragan",
year = "2017",
abstract = "Hybrid systems based on nano hydroxyapatites (HAp) are the subject of numerous studies in preventive and regenerative medicine. Special interests are directed towards the creation of a system based on HAp for use in a nano-oncology. The main objective of this research is directed towards the creation of a system with cytotoxic properties towards the cancer cells with the same time, minimum side effects. Carriers base on core shell of HAp/chitosan-poly(D,L)-lactide-coglycolide (PLGA) loaded with androstane-based cancer inhibitor could be seen as promising drug delivery platforms for selective cancer therapies.
In this study we utilize an emulsification process and freeze drying to load the composite particles based on HAp nanocarrier, chitosane (Ch), PLGA and chitosan oligosaccharide lactate (ChOL) with 17β-hydroxy-17α-picolyl-androst-5-en-3β-acetate (A) and 3β,17β-dihydroxy-16-hydroxymino-androst-5-en (B), a chemotherapeutic derivatives of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormone-dependent cancers (lung, prostate and colon cancer; adeno and cervix carcinoma; etc.).
1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivatives A and B. The thermogravimetric and differential thermal analysis (TGA, DTA) coupled with mass spectrometry was used to qualitatively confirm the drug loading process. FT-IR, XRD, AFM and DSC techniques have confirmed the success of androstane (A and B) loading process in core shell particles base on nano hydroxyapatite. All the synthesized particles were found to be spherical in shape with a uniform size distribution from d50=167 to d50=231 nm. Highly selective anticancer activity was noted towards the human lung carcinoma (A549) by A loaded HAp/Ch-PLGA and towards the human breast adenocarcinoma (MDA-MB-231) by B loaded HAp/ChOL. The obtained results of the DET and MTT tests were in agreement.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "Programme and The Book of Abstracts / Nineteenth Annual Conference YUCOMAT 2017, Herceg Novi, September 4-8, 2017",
title = "Highly selective anticancer activity of core shell particles based on hydroxyapatite, chitosan lactate and different androstane derivatives",
pages = "50-50",
url = "https://hdl.handle.net/21.15107/rcub_dais_15442"
}

Ignjatović, N., Penov Gaši, K., Ajduković, J., Sakač, M., Kuzminac, I., Kojić, V. V., Marković, S.,& Uskoković, D.. (2017). Highly selective anticancer activity of core shell particles based on hydroxyapatite, chitosan lactate and different androstane derivatives. in Programme and The Book of Abstracts / Nineteenth Annual Conference YUCOMAT 2017, Herceg Novi, September 4-8, 2017
Belgrade : Materials Research Society of Serbia., 50-50.
https://hdl.handle.net/21.15107/rcub_dais_15442

Ignjatović N, Penov Gaši K, Ajduković J, Sakač M, Kuzminac I, Kojić VV, Marković S, Uskoković D. Highly selective anticancer activity of core shell particles based on hydroxyapatite, chitosan lactate and different androstane derivatives. in Programme and The Book of Abstracts / Nineteenth Annual Conference YUCOMAT 2017, Herceg Novi, September 4-8, 2017. 2017;:50-50.
https://hdl.handle.net/21.15107/rcub_dais_15442 .

Ignjatović, Nenad, Penov Gaši, Katarina, Ajduković, Jovana, Sakač, Marija, Kuzminac, Ivana, Kojić, Vesna V., Marković, Smilja, Uskoković, Dragan, "Highly selective anticancer activity of core shell particles based on hydroxyapatite, chitosan lactate and different androstane derivatives" in Programme and The Book of Abstracts / Nineteenth Annual Conference YUCOMAT 2017, Herceg Novi, September 4-8, 2017 (2017):50-50,
https://hdl.handle.net/21.15107/rcub_dais_15442 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Penov Gaši, Katarina
AU  - Wu, Victoria
AU  - Ajduković, Jovana
AU  - Kojić, Vesna V.
AU  - Vasiljević Radović, Dana
AU  - Kuzmanović, Maja
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/15974
AB  - In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50=168nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.
PB  - Elsevier
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor
SP  - 629
EP  - 639
VL  - 148
DO  - 10.1016/j.colsurfb.2016.09.041
UR  - https://hdl.handle.net/21.15107/rcub_dais_15974
ER  - 

@article{
author = "Ignjatović, Nenad and Penov Gaši, Katarina and Wu, Victoria and Ajduković, Jovana and Kojić, Vesna V. and Vasiljević Radović, Dana and Kuzmanović, Maja and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50=168nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.",
publisher = "Elsevier",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor",
pages = "629-639",
volume = "148",
doi = "10.1016/j.colsurfb.2016.09.041",
url = "https://hdl.handle.net/21.15107/rcub_dais_15974"
}

Ignjatović, N., Penov Gaši, K., Wu, V., Ajduković, J., Kojić, V. V., Vasiljević Radović, D., Kuzmanović, M., Uskoković, V.,& Uskoković, D.. (2016). Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. in Colloids and Surfaces B: Biointerfaces
Elsevier., 148, 629-639.
https://doi.org/10.1016/j.colsurfb.2016.09.041
https://hdl.handle.net/21.15107/rcub_dais_15974

Ignjatović N, Penov Gaši K, Wu V, Ajduković J, Kojić VV, Vasiljević Radović D, Kuzmanović M, Uskoković V, Uskoković D. Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. in Colloids and Surfaces B: Biointerfaces. 2016;148:629-639.
doi:10.1016/j.colsurfb.2016.09.041
https://hdl.handle.net/21.15107/rcub_dais_15974 .

Ignjatović, Nenad, Penov Gaši, Katarina, Wu, Victoria, Ajduković, Jovana, Kojić, Vesna V., Vasiljević Radović, Dana, Kuzmanović, Maja, Uskoković, Vuk, Uskoković, Dragan, "Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor" in Colloids and Surfaces B: Biointerfaces, 148 (2016):629-639,
https://doi.org/10.1016/j.colsurfb.2016.09.041 .,
https://hdl.handle.net/21.15107/rcub_dais_15974 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Penov Gaši, Katarina
AU  - Wu, Victoria
AU  - Ajduković, Jovana
AU  - Kojić, Vesna
AU  - Vasiljević Radović, Dana
AU  - Kuzmanović, Maja
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/15984
AB  - In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50 = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46 ± 2%), while simultaneously preserving high viability (83 ± 3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor
SP  - 629
EP  - 639
VL  - 148
DO  - 10.1016/j.colsurfb.2016.09.041
UR  - https://hdl.handle.net/21.15107/rcub_dais_15984
ER  - 

@article{
author = "Ignjatović, Nenad and Penov Gaši, Katarina and Wu, Victoria and Ajduković, Jovana and Kojić, Vesna and Vasiljević Radović, Dana and Kuzmanović, Maja and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50 = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46 ± 2%), while simultaneously preserving high viability (83 ± 3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor",
pages = "629-639",
volume = "148",
doi = "10.1016/j.colsurfb.2016.09.041",
url = "https://hdl.handle.net/21.15107/rcub_dais_15984"
}

Ignjatović, N., Penov Gaši, K., Wu, V., Ajduković, J., Kojić, V., Vasiljević Radović, D., Kuzmanović, M., Uskoković, V.,& Uskoković, D.. (2016). Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. in Colloids and Surfaces B: Biointerfaces, 148, 629-639.
https://doi.org/10.1016/j.colsurfb.2016.09.041
https://hdl.handle.net/21.15107/rcub_dais_15984

Ignjatović N, Penov Gaši K, Wu V, Ajduković J, Kojić V, Vasiljević Radović D, Kuzmanović M, Uskoković V, Uskoković D. Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. in Colloids and Surfaces B: Biointerfaces. 2016;148:629-639.
doi:10.1016/j.colsurfb.2016.09.041
https://hdl.handle.net/21.15107/rcub_dais_15984 .

Ignjatović, Nenad, Penov Gaši, Katarina, Wu, Victoria, Ajduković, Jovana, Kojić, Vesna, Vasiljević Radović, Dana, Kuzmanović, Maja, Uskoković, Vuk, Uskoković, Dragan, "Selective anticancer activity of hydroxyapatite/chitosan-poly(D,L)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor" in Colloids and Surfaces B: Biointerfaces, 148 (2016):629-639,
https://doi.org/10.1016/j.colsurfb.2016.09.041 .,
https://hdl.handle.net/21.15107/rcub_dais_15984 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Penov Gaši, Katarina
AU  - Wu, Victoria
AU  - Ajduković, Jovana
AU  - Kojić, Vesna
AU  - Vasiljević Radović, Dana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/896
AB  - The applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Previously we have shown that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous administration into mice. For this purpose radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for radiolabeling of particles and investigation of their biodistribution. In this study we utilize an emulsification process and freeze drying to load the composite particles based on hydroxyapatite nanocarrier, chitosane and poly(lactic-co-glycolic acid) with 17β- hydroxy-17α-picolyl-androst-5-en-3β-acetate (A), a chemotherapeutic derivative of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormonedependent cancers (adenocarcinoma, prostate cancer, cervix carcinoma, colon cancer, etc.). 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The synthesized particles of A-loaded HAp/Ch-PLGA were found to be spherical in shape with a uniform size distribution of d50=168 nm. The release of A from HAp/Ch-PLGA was sustained, with no burst release or plateauing after three weeks. The obtained results of the DET and MTT tests show that the particles of A-loaded HAp/Ch-PLGA exhibit almost three times higher cytotoxicity towards lung adenocarcinoma cells (A549) than towards healthy cells (MRC5), while at the same time allowing twice as fast recovery of healthy cells. We have also analyzed the period of recovery of healthy, as well as cancer cells, following the treatment with A-loaded HAp/Ch-PLGA. After treatment with A-loaded HAp/Ch-PLGA, healthy cells recover twice as fast as the malignant ones. Immunofluorescent staining of primary fibroblasts interacting with HAp/Ch-PLGA and A-HAp/Ch-PLGA particles demonstrates no negative morphological or proliferative effects on cells.
PB  - Belgrade : Materials Research Society of Serbia
C3  - Programme and The Book of Abstracts / Eighteenth Annual Conference YUCOMAT 2016, Herceg Novi, September 5-10, 2016
T1  - Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_dais_896
ER  - 

@conference{
author = "Ignjatović, Nenad and Penov Gaši, Katarina and Wu, Victoria and Ajduković, Jovana and Kojić, Vesna and Vasiljević Radović, Dana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "The applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Previously we have shown that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous administration into mice. For this purpose radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for radiolabeling of particles and investigation of their biodistribution. In this study we utilize an emulsification process and freeze drying to load the composite particles based on hydroxyapatite nanocarrier, chitosane and poly(lactic-co-glycolic acid) with 17β- hydroxy-17α-picolyl-androst-5-en-3β-acetate (A), a chemotherapeutic derivative of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormonedependent cancers (adenocarcinoma, prostate cancer, cervix carcinoma, colon cancer, etc.). 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The synthesized particles of A-loaded HAp/Ch-PLGA were found to be spherical in shape with a uniform size distribution of d50=168 nm. The release of A from HAp/Ch-PLGA was sustained, with no burst release or plateauing after three weeks. The obtained results of the DET and MTT tests show that the particles of A-loaded HAp/Ch-PLGA exhibit almost three times higher cytotoxicity towards lung adenocarcinoma cells (A549) than towards healthy cells (MRC5), while at the same time allowing twice as fast recovery of healthy cells. We have also analyzed the period of recovery of healthy, as well as cancer cells, following the treatment with A-loaded HAp/Ch-PLGA. After treatment with A-loaded HAp/Ch-PLGA, healthy cells recover twice as fast as the malignant ones. Immunofluorescent staining of primary fibroblasts interacting with HAp/Ch-PLGA and A-HAp/Ch-PLGA particles demonstrates no negative morphological or proliferative effects on cells.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "Programme and The Book of Abstracts / Eighteenth Annual Conference YUCOMAT 2016, Herceg Novi, September 5-10, 2016",
title = "Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_dais_896"
}

Ignjatović, N., Penov Gaši, K., Wu, V., Ajduković, J., Kojić, V., Vasiljević Radović, D., Uskoković, V.,& Uskoković, D.. (2016). Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate. in Programme and The Book of Abstracts / Eighteenth Annual Conference YUCOMAT 2016, Herceg Novi, September 5-10, 2016
Belgrade : Materials Research Society of Serbia., 27-27.
https://hdl.handle.net/21.15107/rcub_dais_896

Ignjatović N, Penov Gaši K, Wu V, Ajduković J, Kojić V, Vasiljević Radović D, Uskoković V, Uskoković D. Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate. in Programme and The Book of Abstracts / Eighteenth Annual Conference YUCOMAT 2016, Herceg Novi, September 5-10, 2016. 2016;:27-27.
https://hdl.handle.net/21.15107/rcub_dais_896 .

Ignjatović, Nenad, Penov Gaši, Katarina, Wu, Victoria, Ajduković, Jovana, Kojić, Vesna, Vasiljević Radović, Dana, Uskoković, Vuk, Uskoković, Dragan, "Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate" in Programme and The Book of Abstracts / Eighteenth Annual Conference YUCOMAT 2016, Herceg Novi, September 5-10, 2016 (2016):27-27,
https://hdl.handle.net/21.15107/rcub_dais_896 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Kuzmanović, Maja
AU  - Penov Gaši, Katarina
AU  - Ajduković, Jovana
AU  - Kojić, Vesna
AU  - Uskoković, Dragan
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/826
AB  - In our study, we examined the possibilities for the application of Thermo-Gravimetric Analysis/Differential-Thermal Analysis (DTA/TGA) coupled on-line with mass spectrometry (MS) as a fingerprint for identification purposes in drug loading processes. Androstane derivative 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl acetate (2-OAc) with antitumor activity was loaded in nano hydroxyapatite (HAp) coated with chitosan-poly(D,L)-lactide-co-glycolide (Ch-PLGA) by emulsification and finally freeze-dried. By means of DTA/TGA-MS, it was quickly determined that the form of 2-OAc was the same before and after loading. The observed exothermic and endothermic processes due to the transformation of material with simultaneous analysis of gas products have proven to be successful in the analysis of drug loading processes in multi-component ceramic-polymer carriers. The loading efficiency of 74.7% was determined using the Differential Scanning Calorimetry (DSC) technique. A FT-IR analysis confirmed the qualitative composition of the synthesized 2-OAc-loaded HAp/Ch-PLGA. The in vitro antiproliferative activity was evaluated against human cell lines: lung adenocarcinoma (A549), as well as healthy fetal lung fibroblasts (MRC-5). The results of DET and MTT tests have revealed a high viability of healthy cells MRC-5 (82%) and the death of cancer cells A549 (46%) after a treatment with 2-OAc-loaded HAp/Ch-PLGA.
PB  - Belgrade : Materials Research Society of Serbia
C3  - Programme and The Book of Abstracts / Seventeenth Annual Conference YUCOMAT 205, Herceg Novi, August 31– September 4, 2015
T1  - A Facile Determination Method for an Androstane-based Lung Cancer Inhibitor Loaded in Nano/Micro Particles Based on Hydroxyapatite by Means of DTA/TGA Coupled with On-line Mass Spectrometry
SP  - 35
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_dais_826
ER  - 

@conference{
author = "Ignjatović, Nenad and Kuzmanović, Maja and Penov Gaši, Katarina and Ajduković, Jovana and Kojić, Vesna and Uskoković, Dragan",
year = "2015",
abstract = "In our study, we examined the possibilities for the application of Thermo-Gravimetric Analysis/Differential-Thermal Analysis (DTA/TGA) coupled on-line with mass spectrometry (MS) as a fingerprint for identification purposes in drug loading processes. Androstane derivative 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl acetate (2-OAc) with antitumor activity was loaded in nano hydroxyapatite (HAp) coated with chitosan-poly(D,L)-lactide-co-glycolide (Ch-PLGA) by emulsification and finally freeze-dried. By means of DTA/TGA-MS, it was quickly determined that the form of 2-OAc was the same before and after loading. The observed exothermic and endothermic processes due to the transformation of material with simultaneous analysis of gas products have proven to be successful in the analysis of drug loading processes in multi-component ceramic-polymer carriers. The loading efficiency of 74.7% was determined using the Differential Scanning Calorimetry (DSC) technique. A FT-IR analysis confirmed the qualitative composition of the synthesized 2-OAc-loaded HAp/Ch-PLGA. The in vitro antiproliferative activity was evaluated against human cell lines: lung adenocarcinoma (A549), as well as healthy fetal lung fibroblasts (MRC-5). The results of DET and MTT tests have revealed a high viability of healthy cells MRC-5 (82%) and the death of cancer cells A549 (46%) after a treatment with 2-OAc-loaded HAp/Ch-PLGA.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "Programme and The Book of Abstracts / Seventeenth Annual Conference YUCOMAT 205, Herceg Novi, August 31– September 4, 2015",
title = "A Facile Determination Method for an Androstane-based Lung Cancer Inhibitor Loaded in Nano/Micro Particles Based on Hydroxyapatite by Means of DTA/TGA Coupled with On-line Mass Spectrometry",
pages = "35-35",
url = "https://hdl.handle.net/21.15107/rcub_dais_826"
}

Ignjatović, N., Kuzmanović, M., Penov Gaši, K., Ajduković, J., Kojić, V.,& Uskoković, D.. (2015). A Facile Determination Method for an Androstane-based Lung Cancer Inhibitor Loaded in Nano/Micro Particles Based on Hydroxyapatite by Means of DTA/TGA Coupled with On-line Mass Spectrometry. in Programme and The Book of Abstracts / Seventeenth Annual Conference YUCOMAT 205, Herceg Novi, August 31– September 4, 2015
Belgrade : Materials Research Society of Serbia., 35-35.
https://hdl.handle.net/21.15107/rcub_dais_826

Ignjatović N, Kuzmanović M, Penov Gaši K, Ajduković J, Kojić V, Uskoković D. A Facile Determination Method for an Androstane-based Lung Cancer Inhibitor Loaded in Nano/Micro Particles Based on Hydroxyapatite by Means of DTA/TGA Coupled with On-line Mass Spectrometry. in Programme and The Book of Abstracts / Seventeenth Annual Conference YUCOMAT 205, Herceg Novi, August 31– September 4, 2015. 2015;:35-35.
https://hdl.handle.net/21.15107/rcub_dais_826 .

Ignjatović, Nenad, Kuzmanović, Maja, Penov Gaši, Katarina, Ajduković, Jovana, Kojić, Vesna, Uskoković, Dragan, "A Facile Determination Method for an Androstane-based Lung Cancer Inhibitor Loaded in Nano/Micro Particles Based on Hydroxyapatite by Means of DTA/TGA Coupled with On-line Mass Spectrometry" in Programme and The Book of Abstracts / Seventeenth Annual Conference YUCOMAT 205, Herceg Novi, August 31– September 4, 2015 (2015):35-35,
https://hdl.handle.net/21.15107/rcub_dais_826 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Kojić, Vesna
AU  - Bokurov, Miloš
AU  - Srdić, Vladimir
AU  - Krnojelac, Dijana
AU  - Selaković, Srećko
AU  - Uskoković, Dragan
PY  - 2006
UR  - https://dais.sanu.ac.rs/123456789/14367
AB  - Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).
PB  - Wiley
T2  - Microscopy Research and Technique
T1  - Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials
SP  - 976
EP  - 982
VL  - 69
IS  - 12
DO  - 10.1002/jemt.20374
UR  - https://hdl.handle.net/21.15107/rcub_dais_14367
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Kojić, Vesna and Bokurov, Miloš and Srdić, Vladimir and Krnojelac, Dijana and Selaković, Srećko and Uskoković, Dragan",
year = "2006",
abstract = "Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).",
publisher = "Wiley",
journal = "Microscopy Research and Technique",
title = "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials",
pages = "976-982",
volume = "69",
number = "12",
doi = "10.1002/jemt.20374",
url = "https://hdl.handle.net/21.15107/rcub_dais_14367"
}

Ignjatović, N., Ninkov, P., Kojić, V., Bokurov, M., Srdić, V., Krnojelac, D., Selaković, S.,& Uskoković, D.. (2006). Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. in Microscopy Research and Technique
Wiley., 69(12), 976-982.
https://doi.org/10.1002/jemt.20374
https://hdl.handle.net/21.15107/rcub_dais_14367

Ignjatović N, Ninkov P, Kojić V, Bokurov M, Srdić V, Krnojelac D, Selaković S, Uskoković D. Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. in Microscopy Research and Technique. 2006;69(12):976-982.
doi:10.1002/jemt.20374
https://hdl.handle.net/21.15107/rcub_dais_14367 .

Ignjatović, Nenad, Ninkov, Petar, Kojić, Vesna, Bokurov, Miloš, Srdić, Vladimir, Krnojelac, Dijana, Selaković, Srećko, Uskoković, Dragan, "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials" in Microscopy Research and Technique, 69, no. 12 (2006):976-982,
https://doi.org/10.1002/jemt.20374 .,
https://hdl.handle.net/21.15107/rcub_dais_14367 .