TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Stojanović, Zoran
AU  - Pekmezović, Marina
AU  - Veljović, Đorđe
AU  - O’Connor, Kevin
AU  - Malagurski, Ivana
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/12954
AB  - Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7–8 µm in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 × MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.
PB  - MDPI
T2  - Pharmaceutics
T1  - Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model
SP  - 696
VL  - 14
IS  - 4
DO  - 10.3390/pharmaceutics14040696
UR  - https://hdl.handle.net/21.15107/rcub_dais_12954
ER  - 

@article{
author = "Pavić, Aleksandar and Stojanović, Zoran and Pekmezović, Marina and Veljović, Đorđe and O’Connor, Kevin and Malagurski, Ivana and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7–8 µm in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 × MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model",
pages = "696",
volume = "14",
number = "4",
doi = "10.3390/pharmaceutics14040696",
url = "https://hdl.handle.net/21.15107/rcub_dais_12954"
}

Pavić, A., Stojanović, Z., Pekmezović, M., Veljović, Đ., O’Connor, K., Malagurski, I.,& Nikodinović-Runić, J.. (2022). Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics
MDPI., 14(4), 696.
https://doi.org/10.3390/pharmaceutics14040696
https://hdl.handle.net/21.15107/rcub_dais_12954

Pavić A, Stojanović Z, Pekmezović M, Veljović Đ, O’Connor K, Malagurski I, Nikodinović-Runić J. Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics. 2022;14(4):696.
doi:10.3390/pharmaceutics14040696
https://hdl.handle.net/21.15107/rcub_dais_12954 .

Pavić, Aleksandar, Stojanović, Zoran, Pekmezović, Marina, Veljović, Đorđe, O’Connor, Kevin, Malagurski, Ivana, Nikodinović-Runić, Jasmina, "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model" in Pharmaceutics, 14, no. 4 (2022):696,
https://doi.org/10.3390/pharmaceutics14040696 .,
https://hdl.handle.net/21.15107/rcub_dais_12954 .

TY  - JOUR
AU  - Janićijević, Željko
AU  - Stanković, Ana
AU  - Žegura, Bojana
AU  - Veljović, Đorđe
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12339
AB  - We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.
PB  - Springer Science and Business Media B.V.
T2  - Journal of Nanoparticle Research
T1  - Safe-by-design gelatin-modified zinc oxide nanoparticles
VL  - 23
IS  - 9
DO  - 10.1007/s11051-021-05312-3
UR  - https://hdl.handle.net/21.15107/rcub_dais_12339
ER  - 

@article{
author = "Janićijević, Željko and Stanković, Ana and Žegura, Bojana and Veljović, Đorđe and Đekić, Ljiljana and Krajišnik, Danina and Filipič, Metka and Stevanović, Magdalena",
year = "2021",
abstract = "We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.",
publisher = "Springer Science and Business Media B.V.",
journal = "Journal of Nanoparticle Research",
title = "Safe-by-design gelatin-modified zinc oxide nanoparticles",
volume = "23",
number = "9",
doi = "10.1007/s11051-021-05312-3",
url = "https://hdl.handle.net/21.15107/rcub_dais_12339"
}

Janićijević, Ž., Stanković, A., Žegura, B., Veljović, Đ., Đekić, L., Krajišnik, D., Filipič, M.,& Stevanović, M.. (2021). Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research
Springer Science and Business Media B.V.., 23(9).
https://doi.org/10.1007/s11051-021-05312-3
https://hdl.handle.net/21.15107/rcub_dais_12339

Janićijević Ž, Stanković A, Žegura B, Veljović Đ, Đekić L, Krajišnik D, Filipič M, Stevanović M. Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research. 2021;23(9).
doi:10.1007/s11051-021-05312-3
https://hdl.handle.net/21.15107/rcub_dais_12339 .

Janićijević, Željko, Stanković, Ana, Žegura, Bojana, Veljović, Đorđe, Đekić, Ljiljana, Krajišnik, Danina, Filipič, Metka, Stevanović, Magdalena, "Safe-by-design gelatin-modified zinc oxide nanoparticles" in Journal of Nanoparticle Research, 23, no. 9 (2021),
https://doi.org/10.1007/s11051-021-05312-3 .,
https://hdl.handle.net/21.15107/rcub_dais_12339 .

TY  - JOUR
AU  - Janićijević, Željko
AU  - Stanković, Ana
AU  - Žegura, Bojana
AU  - Veljović, Đorđe
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11876
AB  - We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.
PB  - Springer Science and Business Media B.V.
T2  - Journal of Nanoparticle Research
T1  - Safe-by-design gelatin-modified zinc oxide nanoparticles
VL  - 23
IS  - 9
DO  - 10.1007/s11051-021-05312-3
UR  - https://hdl.handle.net/21.15107/rcub_dais_11876
ER  - 

@article{
author = "Janićijević, Željko and Stanković, Ana and Žegura, Bojana and Veljović, Đorđe and Đekić, Ljiljana and Krajišnik, Danina and Filipič, Metka and Stevanović, Magdalena",
year = "2021",
abstract = "We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.",
publisher = "Springer Science and Business Media B.V.",
journal = "Journal of Nanoparticle Research",
title = "Safe-by-design gelatin-modified zinc oxide nanoparticles",
volume = "23",
number = "9",
doi = "10.1007/s11051-021-05312-3",
url = "https://hdl.handle.net/21.15107/rcub_dais_11876"
}

Janićijević, Ž., Stanković, A., Žegura, B., Veljović, Đ., Đekić, L., Krajišnik, D., Filipič, M.,& Stevanović, M.. (2021). Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research
Springer Science and Business Media B.V.., 23(9).
https://doi.org/10.1007/s11051-021-05312-3
https://hdl.handle.net/21.15107/rcub_dais_11876

Janićijević Ž, Stanković A, Žegura B, Veljović Đ, Đekić L, Krajišnik D, Filipič M, Stevanović M. Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research. 2021;23(9).
doi:10.1007/s11051-021-05312-3
https://hdl.handle.net/21.15107/rcub_dais_11876 .

Janićijević, Željko, Stanković, Ana, Žegura, Bojana, Veljović, Đorđe, Đekić, Ljiljana, Krajišnik, Danina, Filipič, Metka, Stevanović, Magdalena, "Safe-by-design gelatin-modified zinc oxide nanoparticles" in Journal of Nanoparticle Research, 23, no. 9 (2021),
https://doi.org/10.1007/s11051-021-05312-3 .,
https://hdl.handle.net/21.15107/rcub_dais_11876 .

TY  - JOUR
AU  - Miladinović, Marija R.
AU  - Zdujić, Miodrag
AU  - Veljović, Đorđe
AU  - Krstić, Jugoslav B.
AU  - Banković Ilić, Ivana B.
AU  - Veljković, Vlada B.
AU  - Stamenković, Olivera S.
PY  - 2020
UR  - https://dais.sanu.ac.rs/123456789/6903
AB  - The catalytic activity of the walnut shell ash was investigated in the biodiesel production by the sunflower oil methanolysis. The catalyst was characterized by the TG-DTA, XRD, Hg porosimetry, N2 physisorption, SEM, and Hammett method. In addition, the effects of the catalyst loading and the methanol-to-oil molar ratio on the methyl esters synthesis were tested at the reaction temperature of 60 °C. The walnut shell ash provided a very fast reaction and a high FAME content (over 98%). As the reaction occurred in the absence of triacylglycerols mass transfer limitation, the pseudo-first-order model was employed for describing the kinetics of the reaction. The catalyst was successfully reused four times after the regeneration of the catalytic activity by recalcination at 800 °C.
PB  - Elsevier
T2  - Renewable Energy
T1  - Valorization of walnut shell ash as a catalyst for biodiesel production
SP  - 1033
EP  - 1043
VL  - 147
DO  - 10.1016/j.renene.2019.09.056
UR  - https://hdl.handle.net/21.15107/rcub_dais_6903
ER  - 

@article{
author = "Miladinović, Marija R. and Zdujić, Miodrag and Veljović, Đorđe and Krstić, Jugoslav B. and Banković Ilić, Ivana B. and Veljković, Vlada B. and Stamenković, Olivera S.",
year = "2020",
abstract = "The catalytic activity of the walnut shell ash was investigated in the biodiesel production by the sunflower oil methanolysis. The catalyst was characterized by the TG-DTA, XRD, Hg porosimetry, N2 physisorption, SEM, and Hammett method. In addition, the effects of the catalyst loading and the methanol-to-oil molar ratio on the methyl esters synthesis were tested at the reaction temperature of 60 °C. The walnut shell ash provided a very fast reaction and a high FAME content (over 98%). As the reaction occurred in the absence of triacylglycerols mass transfer limitation, the pseudo-first-order model was employed for describing the kinetics of the reaction. The catalyst was successfully reused four times after the regeneration of the catalytic activity by recalcination at 800 °C.",
publisher = "Elsevier",
journal = "Renewable Energy",
title = "Valorization of walnut shell ash as a catalyst for biodiesel production",
pages = "1033-1043",
volume = "147",
doi = "10.1016/j.renene.2019.09.056",
url = "https://hdl.handle.net/21.15107/rcub_dais_6903"
}

Miladinović, M. R., Zdujić, M., Veljović, Đ., Krstić, J. B., Banković Ilić, I. B., Veljković, V. B.,& Stamenković, O. S.. (2020). Valorization of walnut shell ash as a catalyst for biodiesel production. in Renewable Energy
Elsevier., 147, 1033-1043.
https://doi.org/10.1016/j.renene.2019.09.056
https://hdl.handle.net/21.15107/rcub_dais_6903

Miladinović MR, Zdujić M, Veljović Đ, Krstić JB, Banković Ilić IB, Veljković VB, Stamenković OS. Valorization of walnut shell ash as a catalyst for biodiesel production. in Renewable Energy. 2020;147:1033-1043.
doi:10.1016/j.renene.2019.09.056
https://hdl.handle.net/21.15107/rcub_dais_6903 .

Miladinović, Marija R., Zdujić, Miodrag, Veljović, Đorđe, Krstić, Jugoslav B., Banković Ilić, Ivana B., Veljković, Vlada B., Stamenković, Olivera S., "Valorization of walnut shell ash as a catalyst for biodiesel production" in Renewable Energy, 147 (2020):1033-1043,
https://doi.org/10.1016/j.renene.2019.09.056 .,
https://hdl.handle.net/21.15107/rcub_dais_6903 .

TY  - JOUR
AU  - Miladinović, Marija R.
AU  - Zdujić, Miodrag
AU  - Veljović, Đorđe
AU  - Krstić, Jugoslav B.
AU  - Banković Ilić, Ivana B.
AU  - Veljković, Vlada B.
AU  - Stamenković, Olivera S.
PY  - 2020
UR  - http://cer.ihtm.bg.ac.rs/handle/123456789/3217
UR  - https://dais.sanu.ac.rs/123456789/6717
AB  - The catalytic activity of the walnut shell ash was investigated in the biodiesel production by the sunflower oil methanolysis. The catalyst was characterized by the TG-DTA, XRD, Hg porosimetry, N2 physisorption, SEM, and Hammett method. In addition, the effects of the catalyst loading and the methanol-to-oil molar ratio on the methyl esters synthesis were tested at the reaction temperature of 60 °C. The walnut shell ash provided a very fast reaction and a high FAME content (over 98%). As the reaction occurred in the absence of triacylglycerols mass transfer limitation, the pseudo-first-order model was employed for describing the kinetics of the reaction. The catalyst was successfully reused four times after the regeneration of the catalytic activity by recalcination at 800 °C.
PB  - Elsevier
T2  - Renewable Energy
T1  - Valorization of walnut shell ash as a catalyst for biodiesel production
SP  - 1033
EP  - 1043
VL  - 147
DO  - 10.1016/j.renene.2019.09.056
UR  - https://hdl.handle.net/21.15107/rcub_dais_6717
ER  - 

@article{
author = "Miladinović, Marija R. and Zdujić, Miodrag and Veljović, Đorđe and Krstić, Jugoslav B. and Banković Ilić, Ivana B. and Veljković, Vlada B. and Stamenković, Olivera S.",
year = "2020",
abstract = "The catalytic activity of the walnut shell ash was investigated in the biodiesel production by the sunflower oil methanolysis. The catalyst was characterized by the TG-DTA, XRD, Hg porosimetry, N2 physisorption, SEM, and Hammett method. In addition, the effects of the catalyst loading and the methanol-to-oil molar ratio on the methyl esters synthesis were tested at the reaction temperature of 60 °C. The walnut shell ash provided a very fast reaction and a high FAME content (over 98%). As the reaction occurred in the absence of triacylglycerols mass transfer limitation, the pseudo-first-order model was employed for describing the kinetics of the reaction. The catalyst was successfully reused four times after the regeneration of the catalytic activity by recalcination at 800 °C.",
publisher = "Elsevier",
journal = "Renewable Energy",
title = "Valorization of walnut shell ash as a catalyst for biodiesel production",
pages = "1033-1043",
volume = "147",
doi = "10.1016/j.renene.2019.09.056",
url = "https://hdl.handle.net/21.15107/rcub_dais_6717"
}

Miladinović, M. R., Zdujić, M., Veljović, Đ., Krstić, J. B., Banković Ilić, I. B., Veljković, V. B.,& Stamenković, O. S.. (2020). Valorization of walnut shell ash as a catalyst for biodiesel production. in Renewable Energy
Elsevier., 147, 1033-1043.
https://doi.org/10.1016/j.renene.2019.09.056
https://hdl.handle.net/21.15107/rcub_dais_6717

Miladinović MR, Zdujić M, Veljović Đ, Krstić JB, Banković Ilić IB, Veljković VB, Stamenković OS. Valorization of walnut shell ash as a catalyst for biodiesel production. in Renewable Energy. 2020;147:1033-1043.
doi:10.1016/j.renene.2019.09.056
https://hdl.handle.net/21.15107/rcub_dais_6717 .

Miladinović, Marija R., Zdujić, Miodrag, Veljović, Đorđe, Krstić, Jugoslav B., Banković Ilić, Ivana B., Veljković, Vlada B., Stamenković, Olivera S., "Valorization of walnut shell ash as a catalyst for biodiesel production" in Renewable Energy, 147 (2020):1033-1043,
https://doi.org/10.1016/j.renene.2019.09.056 .,
https://hdl.handle.net/21.15107/rcub_dais_6717 .

TY  - DATA
AU  - Janićijević, Željko
AU  - Vujčić, Ivica
AU  - Veljović, Đorđe
AU  - Vujisić, Miloš
AU  - Radovanović, Filip
PY  - 2020
UR  - https://dais.sanu.ac.rs/123456789/6944
AB  - Composite hydrogels capable of controlled drug delivery via ion exchange are an interesting group of materials for the construction of implantable drug reservoirs for electrically charged drugs. In this study, we synthesized composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) (PLGA-PAA) hydrogels by sequential application of UV or gamma irradiation and traditional phase inversion. Physicochemical properties of the composite PLGA-PAA hydrogels were investigated using Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). We examined the ion exchange capacity (IEC) and swelling behavior of these materials to determine their potential as drug reservoirs. Composite PLGA-PAA hydrogel synthesized using UV irradiation (UV-PLGA-PAA) exhibited a porous microstructure with submicron-sized hydrogel-rich aggregates and homogeneous chemical composition. Swelling behavior and IEC of this material were highly reproducible. Composite PLGA-PAA hydrogels synthesized using gamma irradiation (G-PLGA-PAAs) had a less uniform microstructure with larger pores and micron-sized hydrogel-rich aggregates while exhibiting rather inhomogeneous chemical composition. These materials showed superior swelling properties, but a more variable IEC, compared to the material fabricated using UV irradiation. Results of DSC analysis showed a dose-dependent decrease in glass transition temperature for G-PLGA-PAAs indicating the effects of PLGA chain scission. Our findings indicate that gamma irradiation is a possible alternative to UV irradiation in the synthesis of composite PLGA-PAA hydrogels which can modify or control important material properties. However, the synthesis protocol using gamma irradiation should be further optimized to improve the IEC reproducibility. In our future research, we will investigate the in vitro release of charged drugs from synthesized composite PLGA-PAA hydrogels under physiological conditions.
T2  - Radiation Physics and Chemistry
T1  - Supplementary data for the article: Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M., Radovanović, F., 2020. Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. Radiation Physics and Chemistry 166, 108466. https://doi.org/10.1016/j.radphyschem.2019.108466
VL  - 166
UR  - https://hdl.handle.net/21.15107/rcub_dais_6944
ER  - 

@misc{
author = "Janićijević, Željko and Vujčić, Ivica and Veljović, Đorđe and Vujisić, Miloš and Radovanović, Filip",
year = "2020",
abstract = "Composite hydrogels capable of controlled drug delivery via ion exchange are an interesting group of materials for the construction of implantable drug reservoirs for electrically charged drugs. In this study, we synthesized composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) (PLGA-PAA) hydrogels by sequential application of UV or gamma irradiation and traditional phase inversion. Physicochemical properties of the composite PLGA-PAA hydrogels were investigated using Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). We examined the ion exchange capacity (IEC) and swelling behavior of these materials to determine their potential as drug reservoirs. Composite PLGA-PAA hydrogel synthesized using UV irradiation (UV-PLGA-PAA) exhibited a porous microstructure with submicron-sized hydrogel-rich aggregates and homogeneous chemical composition. Swelling behavior and IEC of this material were highly reproducible. Composite PLGA-PAA hydrogels synthesized using gamma irradiation (G-PLGA-PAAs) had a less uniform microstructure with larger pores and micron-sized hydrogel-rich aggregates while exhibiting rather inhomogeneous chemical composition. These materials showed superior swelling properties, but a more variable IEC, compared to the material fabricated using UV irradiation. Results of DSC analysis showed a dose-dependent decrease in glass transition temperature for G-PLGA-PAAs indicating the effects of PLGA chain scission. Our findings indicate that gamma irradiation is a possible alternative to UV irradiation in the synthesis of composite PLGA-PAA hydrogels which can modify or control important material properties. However, the synthesis protocol using gamma irradiation should be further optimized to improve the IEC reproducibility. In our future research, we will investigate the in vitro release of charged drugs from synthesized composite PLGA-PAA hydrogels under physiological conditions.",
journal = "Radiation Physics and Chemistry",
title = "Supplementary data for the article: Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M., Radovanović, F., 2020. Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. Radiation Physics and Chemistry 166, 108466. https://doi.org/10.1016/j.radphyschem.2019.108466",
volume = "166",
url = "https://hdl.handle.net/21.15107/rcub_dais_6944"
}

Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M.,& Radovanović, F.. (2020). Supplementary data for the article: Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M., Radovanović, F., 2020. Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. Radiation Physics and Chemistry 166, 108466. https://doi.org/10.1016/j.radphyschem.2019.108466. in Radiation Physics and Chemistry, 166.
https://hdl.handle.net/21.15107/rcub_dais_6944

Janićijević Ž, Vujčić I, Veljović Đ, Vujisić M, Radovanović F. Supplementary data for the article: Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M., Radovanović, F., 2020. Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. Radiation Physics and Chemistry 166, 108466. https://doi.org/10.1016/j.radphyschem.2019.108466. in Radiation Physics and Chemistry. 2020;166.
https://hdl.handle.net/21.15107/rcub_dais_6944 .

Janićijević, Željko, Vujčić, Ivica, Veljović, Đorđe, Vujisić, Miloš, Radovanović, Filip, "Supplementary data for the article: Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M., Radovanović, F., 2020. Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. Radiation Physics and Chemistry 166, 108466. https://doi.org/10.1016/j.radphyschem.2019.108466" in Radiation Physics and Chemistry, 166 (2020),
https://hdl.handle.net/21.15107/rcub_dais_6944 .

TY  - JOUR
AU  - Janićijević, Željko
AU  - Vujčić, Ivica
AU  - Veljović, Đorđe
AU  - Vujisić, Miloš
AU  - Radovanović, Filip
PY  - 2020
UR  - http://www.sciencedirect.com/science/article/pii/S0969806X19301380
UR  - https://dais.sanu.ac.rs/123456789/6943
AB  - Composite hydrogels capable of controlled drug delivery via ion exchange are an interesting group of materials for the construction of implantable drug reservoirs for electrically charged drugs. In this study, we synthesized composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) (PLGA-PAA) hydrogels by sequential application of UV or gamma irradiation and traditional phase inversion. Physicochemical properties of the composite PLGA-PAA hydrogels were investigated using Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). We examined the ion exchange capacity (IEC) and swelling behavior of these materials to determine their potential as drug reservoirs. Composite PLGA-PAA hydrogel synthesized using UV irradiation (UV-PLGA-PAA) exhibited a porous microstructure with submicron-sized hydrogel-rich aggregates and homogeneous chemical composition. Swelling behavior and IEC of this material were highly reproducible. Composite PLGA-PAA hydrogels synthesized using gamma irradiation (G-PLGA-PAAs) had a less uniform microstructure with larger pores and micron-sized hydrogel-rich aggregates while exhibiting rather inhomogeneous chemical composition. These materials showed superior swelling properties, but a more variable IEC, compared to the material fabricated using UV irradiation. Results of DSC analysis showed a dose-dependent decrease in glass transition temperature for G-PLGA-PAAs indicating the effects of PLGA chain scission. Our findings indicate that gamma irradiation is a possible alternative to UV irradiation in the synthesis of composite PLGA-PAA hydrogels which can modify or control important material properties. However, the synthesis protocol using gamma irradiation should be further optimized to improve the IEC reproducibility. In our future research, we will investigate the in vitro release of charged drugs from synthesized composite PLGA-PAA hydrogels under physiological conditions.
PB  - Elsevier
T2  - Radiation Physics and Chemistry
T1  - Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties
SP  - 108466
VL  - 166
DO  - 10.1016/j.radphyschem.2019.108466
UR  - https://hdl.handle.net/21.15107/rcub_dais_6943
ER  - 

@article{
author = "Janićijević, Željko and Vujčić, Ivica and Veljović, Đorđe and Vujisić, Miloš and Radovanović, Filip",
year = "2020",
abstract = "Composite hydrogels capable of controlled drug delivery via ion exchange are an interesting group of materials for the construction of implantable drug reservoirs for electrically charged drugs. In this study, we synthesized composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) (PLGA-PAA) hydrogels by sequential application of UV or gamma irradiation and traditional phase inversion. Physicochemical properties of the composite PLGA-PAA hydrogels were investigated using Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). We examined the ion exchange capacity (IEC) and swelling behavior of these materials to determine their potential as drug reservoirs. Composite PLGA-PAA hydrogel synthesized using UV irradiation (UV-PLGA-PAA) exhibited a porous microstructure with submicron-sized hydrogel-rich aggregates and homogeneous chemical composition. Swelling behavior and IEC of this material were highly reproducible. Composite PLGA-PAA hydrogels synthesized using gamma irradiation (G-PLGA-PAAs) had a less uniform microstructure with larger pores and micron-sized hydrogel-rich aggregates while exhibiting rather inhomogeneous chemical composition. These materials showed superior swelling properties, but a more variable IEC, compared to the material fabricated using UV irradiation. Results of DSC analysis showed a dose-dependent decrease in glass transition temperature for G-PLGA-PAAs indicating the effects of PLGA chain scission. Our findings indicate that gamma irradiation is a possible alternative to UV irradiation in the synthesis of composite PLGA-PAA hydrogels which can modify or control important material properties. However, the synthesis protocol using gamma irradiation should be further optimized to improve the IEC reproducibility. In our future research, we will investigate the in vitro release of charged drugs from synthesized composite PLGA-PAA hydrogels under physiological conditions.",
publisher = "Elsevier",
journal = "Radiation Physics and Chemistry",
title = "Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties",
pages = "108466",
volume = "166",
doi = "10.1016/j.radphyschem.2019.108466",
url = "https://hdl.handle.net/21.15107/rcub_dais_6943"
}

Janićijević, Ž., Vujčić, I., Veljović, Đ., Vujisić, M.,& Radovanović, F.. (2020). Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. in Radiation Physics and Chemistry
Elsevier., 166, 108466.
https://doi.org/10.1016/j.radphyschem.2019.108466
https://hdl.handle.net/21.15107/rcub_dais_6943

Janićijević Ž, Vujčić I, Veljović Đ, Vujisić M, Radovanović F. Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties. in Radiation Physics and Chemistry. 2020;166:108466.
doi:10.1016/j.radphyschem.2019.108466
https://hdl.handle.net/21.15107/rcub_dais_6943 .

Janićijević, Željko, Vujčić, Ivica, Veljović, Đorđe, Vujisić, Miloš, Radovanović, Filip, "Composite poly(DL-lactide-co-glycolide)/poly(acrylic acid) hydrogels synthesized using UV and gamma irradiation: comparison of material properties" in Radiation Physics and Chemistry, 166 (2020):108466,
https://doi.org/10.1016/j.radphyschem.2019.108466 .,
https://hdl.handle.net/21.15107/rcub_dais_6943 .

TY  - JOUR
AU  - Veljović, Đorđe
AU  - Matić, Tamara
AU  - Stamenić, Tanja
AU  - Kojić, Vesna
AU  - Dimitrijević Branković, Suzana
AU  - Lukić, Miodrag J.
AU  - Jevtić, Sanja
AU  - Radovanović, Željko
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/S0272884219320413
UR  - https://dais.sanu.ac.rs/123456789/6948
AB  - The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.
PB  - Elsevier
T2  - Ceramics International
T1  - Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity
SP  - 22029
SP  - 22039
VL  - 45
IS  - 17, Part A
DO  - 10.1016/j.ceramint.2019.07.219
UR  - https://hdl.handle.net/21.15107/rcub_dais_6948
ER  - 

@article{
author = "Veljović, Đorđe and Matić, Tamara and Stamenić, Tanja and Kojić, Vesna and Dimitrijević Branković, Suzana and Lukić, Miodrag J. and Jevtić, Sanja and Radovanović, Željko and Petrović, Rada and Janaćković, Đorđe",
year = "2019",
abstract = "The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.",
publisher = "Elsevier",
journal = "Ceramics International",
title = "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity",
pages = "22029-22039",
volume = "45",
number = "17, Part A",
doi = "10.1016/j.ceramint.2019.07.219",
url = "https://hdl.handle.net/21.15107/rcub_dais_6948"
}

Veljović, Đ., Matić, T., Stamenić, T., Kojić, V., Dimitrijević Branković, S., Lukić, M. J., Jevtić, S., Radovanović, Ž., Petrović, R.,& Janaćković, Đ.. (2019). Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International
Elsevier., 45(17, Part A), 22029.
https://doi.org/10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6948

Veljović Đ, Matić T, Stamenić T, Kojić V, Dimitrijević Branković S, Lukić MJ, Jevtić S, Radovanović Ž, Petrović R, Janaćković Đ. Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International. 2019;45(17, Part A):22029.
doi:10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6948 .

Veljović, Đorđe, Matić, Tamara, Stamenić, Tanja, Kojić, Vesna, Dimitrijević Branković, Suzana, Lukić, Miodrag J., Jevtić, Sanja, Radovanović, Željko, Petrović, Rada, Janaćković, Đorđe, "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity" in Ceramics International, 45, no. 17, Part A (2019):22029,
https://doi.org/10.1016/j.ceramint.2019.07.219 .,
https://hdl.handle.net/21.15107/rcub_dais_6948 .

TY  - JOUR
AU  - Veljović, Đorđe
AU  - Matić, Tamara
AU  - Stamenić, Tanja
AU  - Kojić, Vesna
AU  - Dimitrijević Branković, Suzana
AU  - Lukić, Miodrag J.
AU  - Jevtić, Sanja
AU  - Radovanović, Željko
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/S0272884219320413
UR  - https://dais.sanu.ac.rs/123456789/6504
AB  - The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.
PB  - Elsevier
T2  - Ceramics International
T1  - Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity
SP  - 22029
SP  - 22039
VL  - 45
IS  - 17, Part A
DO  - 10.1016/j.ceramint.2019.07.219
UR  - https://hdl.handle.net/21.15107/rcub_dais_6504
ER  - 

@article{
author = "Veljović, Đorđe and Matić, Tamara and Stamenić, Tanja and Kojić, Vesna and Dimitrijević Branković, Suzana and Lukić, Miodrag J. and Jevtić, Sanja and Radovanović, Željko and Petrović, Rada and Janaćković, Đorđe",
year = "2019",
abstract = "The aim of this study was to improve the mechanical properties and to optimize antimicrobial activity of hydroxyapatite (HAP) by simultaneous doping with Mg and Cu ions in order to obtain material that would be able to assist in the bone/tooth healing process, prevent post-implementation infections and provide satisfying values of hardness and fracture toughness for biomedical application. Ion doping was done during the hydrothermal synthesis of HAP powders, whereby the content of Mg ions in the starting solution was varied between 1-20 mol. % with regard to Ca ions, while the amount of Cu ions was kept constant at 0.4 mol. %. The green compacts were sintered for 2 h at temperatures ranging 750–1200 °C depending on the Mg content, chosen in agreement with dilatometry results. Presence of Mg ions was found to favour transition from HAP to β−tricalcium phosphate phase (β−TCP), which enabled formation of biphasic HAP/β−TCP and pure β−TCP phase at 160 °C during hydrothermal synthesis. In vitro investigation of antimicrobial activity against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis showed satisfactory antimicrobial activity. MTT assay performed on MRC-5 and L929 cell lines showed excellent cytocompatibility and cell proliferation. Maximum hardness by Vickers and fracture toughness values, 4.96 GPa and 1.75 MPa m1/2 respectively, were obtained upon addition of 5 mol. % Mg, as a consequence of the lowest grain size and porosity, as well as the highest densification rate. This is, to the best of our knowledge, the highest fracture toughness for HAP or β-TCP ceramics reported thus far.",
publisher = "Elsevier",
journal = "Ceramics International",
title = "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity",
pages = "22029-22039",
volume = "45",
number = "17, Part A",
doi = "10.1016/j.ceramint.2019.07.219",
url = "https://hdl.handle.net/21.15107/rcub_dais_6504"
}

Veljović, Đ., Matić, T., Stamenić, T., Kojić, V., Dimitrijević Branković, S., Lukić, M. J., Jevtić, S., Radovanović, Ž., Petrović, R.,& Janaćković, Đ.. (2019). Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International
Elsevier., 45(17, Part A), 22029.
https://doi.org/10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6504

Veljović Đ, Matić T, Stamenić T, Kojić V, Dimitrijević Branković S, Lukić MJ, Jevtić S, Radovanović Ž, Petrović R, Janaćković Đ. Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity. in Ceramics International. 2019;45(17, Part A):22029.
doi:10.1016/j.ceramint.2019.07.219
https://hdl.handle.net/21.15107/rcub_dais_6504 .

Veljović, Đorđe, Matić, Tamara, Stamenić, Tanja, Kojić, Vesna, Dimitrijević Branković, Suzana, Lukić, Miodrag J., Jevtić, Sanja, Radovanović, Željko, Petrović, Rada, Janaćković, Đorđe, "Mg/Cu co-substituted hydroxyapatite – Biocompatibility, mechanical properties and antimicrobial activity" in Ceramics International, 45, no. 17, Part A (2019):22029,
https://doi.org/10.1016/j.ceramint.2019.07.219 .,
https://hdl.handle.net/21.15107/rcub_dais_6504 .

TY  - CONF
AU  - Janićijević, Željko
AU  - Stanković, Ana
AU  - Žegura, Bojana
AU  - Veljović, Đorđe
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/6968
AB  - Nanostructured zinc oxides are promising materials for numerous biomedical applications where they can serve as therapeutic agents or tools for sensing and imaging. Despite their favorable properties, wider use of zinc oxide nanoparticles in biomedicine is limited by toxicity issues. Therefore, new synthesis approaches should be devised to obtain zinc oxide nanoparticles which are safe-by-design. We present an innovative low-cost wet precipitation synthesis of gelatin modified zinc oxide nanoparticles at the gel/liquid interface. The diffusion of ammonia through the gelatin hydrogels of different porosities induces precipitation of the product in contact with the surface of the aqueous solution of zinc ions. After thermal treatment of the precipitate, adsorbed organic residues of decomposed gelatin act as modifiers of zinc oxide nanoparticles. We characterized the physicochemical properties of obtained gelatin modified zinc oxide nanoparticles by XRD, FTIR, DTA/TG, and SEM. The synthesized nanoparticles show hexagonal wurtzite structure and form flakelike aggregates. FTIR and DTA/TG analyses indicate that the thermal decomposition of adsorbed gelatin depends on the gelatin content of the hydrogel used in the synthesis. We also examined the viability of HepG2 cells, generation of intracellular reactive oxygen species, and genotoxicity using the MTS, DCFH-DA, and alkaline comet assay, respectively. Fabricated gelatin modified zinc oxide nanoparticles show very low toxicity potential at doses relevant for human exposure.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
T1  - Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles
SP  - 3
EP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_dais_6968
ER  - 

@conference{
author = "Janićijević, Željko and Stanković, Ana and Žegura, Bojana and Veljović, Đorđe and Filipič, Metka and Stevanović, Magdalena",
year = "2019",
abstract = "Nanostructured zinc oxides are promising materials for numerous biomedical applications where they can serve as therapeutic agents or tools for sensing and imaging. Despite their favorable properties, wider use of zinc oxide nanoparticles in biomedicine is limited by toxicity issues. Therefore, new synthesis approaches should be devised to obtain zinc oxide nanoparticles which are safe-by-design. We present an innovative low-cost wet precipitation synthesis of gelatin modified zinc oxide nanoparticles at the gel/liquid interface. The diffusion of ammonia through the gelatin hydrogels of different porosities induces precipitation of the product in contact with the surface of the aqueous solution of zinc ions. After thermal treatment of the precipitate, adsorbed organic residues of decomposed gelatin act as modifiers of zinc oxide nanoparticles. We characterized the physicochemical properties of obtained gelatin modified zinc oxide nanoparticles by XRD, FTIR, DTA/TG, and SEM. The synthesized nanoparticles show hexagonal wurtzite structure and form flakelike aggregates. FTIR and DTA/TG analyses indicate that the thermal decomposition of adsorbed gelatin depends on the gelatin content of the hydrogel used in the synthesis. We also examined the viability of HepG2 cells, generation of intracellular reactive oxygen species, and genotoxicity using the MTS, DCFH-DA, and alkaline comet assay, respectively. Fabricated gelatin modified zinc oxide nanoparticles show very low toxicity potential at doses relevant for human exposure.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia",
title = "Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles",
pages = "3-3",
url = "https://hdl.handle.net/21.15107/rcub_dais_6968"
}

Janićijević, Ž., Stanković, A., Žegura, B., Veljović, Đ., Filipič, M.,& Stevanović, M.. (2019). Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 3-3.
https://hdl.handle.net/21.15107/rcub_dais_6968

Janićijević Ž, Stanković A, Žegura B, Veljović Đ, Filipič M, Stevanović M. Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:3-3.
https://hdl.handle.net/21.15107/rcub_dais_6968 .

Janićijević, Željko, Stanković, Ana, Žegura, Bojana, Veljović, Đorđe, Filipič, Metka, Stevanović, Magdalena, "Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles" in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia (2019):3-3,
https://hdl.handle.net/21.15107/rcub_dais_6968 .

TY  - CONF
AU  - Veljović, Đorđe
AU  - Radovanović, Željko
AU  - Rogan, Jelena
AU  - Dapčević, Aleksandra
AU  - Dimitrijević, Suzana
AU  - Dimitrijević Branković, Suzana
AU  - Kojić, Vesna
AU  - Janaćković, Đorđe
PY  - 2018
UR  - https://dais.sanu.ac.rs/123456789/4718
AB  - Bioactive glasses have been used for over three decades in biomedical applications owing to high bioactivity, biocompatibility, as well as the possibility to stimulate regeneration of the bone. The aim of this work was to synthesized bioactive glasses, which contain lithium and strontium, by commercial method melting-quenching, as well as determining the properties, affected by mentioned ions. Differential thermal/thermogravimetric analysis, particle size distribution, energy-dispersive X-ray spectroscopy, inductively coupled plasma optical emission spectrometry, evaluation of the antimicrobial activity, in vitro bioactivity and biocompatibility test and scanning electron microscopy were used for characterization. The results showed that glass transition and crystallization temperatures are decreasing with addition of lithium and strontium ions. Furthermore, the samples showed high inhibition rate of Escherichia coli growth, as well as high bioactivity and biocompatibility. The crystal apatite layer, formed on the surface of synthesized glasses after seven days in simulated body fluid, differs in shape, size and interconnection of the glass particles, which depends on concentration of lithium and strontium ions.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
T1  - Synthesis and characterization of bioactive glass doped with lithium and strontium ions
SP  - 20
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_dais_4718
ER  - 

@conference{
author = "Veljović, Đorđe and Radovanović, Željko and Rogan, Jelena and Dapčević, Aleksandra and Dimitrijević, Suzana and Dimitrijević Branković, Suzana and Kojić, Vesna and Janaćković, Đorđe",
year = "2018",
abstract = "Bioactive glasses have been used for over three decades in biomedical applications owing to high bioactivity, biocompatibility, as well as the possibility to stimulate regeneration of the bone. The aim of this work was to synthesized bioactive glasses, which contain lithium and strontium, by commercial method melting-quenching, as well as determining the properties, affected by mentioned ions. Differential thermal/thermogravimetric analysis, particle size distribution, energy-dispersive X-ray spectroscopy, inductively coupled plasma optical emission spectrometry, evaluation of the antimicrobial activity, in vitro bioactivity and biocompatibility test and scanning electron microscopy were used for characterization. The results showed that glass transition and crystallization temperatures are decreasing with addition of lithium and strontium ions. Furthermore, the samples showed high inhibition rate of Escherichia coli growth, as well as high bioactivity and biocompatibility. The crystal apatite layer, formed on the surface of synthesized glasses after seven days in simulated body fluid, differs in shape, size and interconnection of the glass particles, which depends on concentration of lithium and strontium ions.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia",
title = "Synthesis and characterization of bioactive glass doped with lithium and strontium ions",
pages = "20-20",
url = "https://hdl.handle.net/21.15107/rcub_dais_4718"
}

Veljović, Đ., Radovanović, Ž., Rogan, J., Dapčević, A., Dimitrijević, S., Dimitrijević Branković, S., Kojić, V.,& Janaćković, Đ.. (2018). Synthesis and characterization of bioactive glass doped with lithium and strontium ions. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 20-20.
https://hdl.handle.net/21.15107/rcub_dais_4718

Veljović Đ, Radovanović Ž, Rogan J, Dapčević A, Dimitrijević S, Dimitrijević Branković S, Kojić V, Janaćković Đ. Synthesis and characterization of bioactive glass doped with lithium and strontium ions. in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia. 2018;:20-20.
https://hdl.handle.net/21.15107/rcub_dais_4718 .

Veljović, Đorđe, Radovanović, Željko, Rogan, Jelena, Dapčević, Aleksandra, Dimitrijević, Suzana, Dimitrijević Branković, Suzana, Kojić, Vesna, Janaćković, Đorđe, "Synthesis and characterization of bioactive glass doped with lithium and strontium ions" in Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia (2018):20-20,
https://hdl.handle.net/21.15107/rcub_dais_4718 .

TY  - JOUR
AU  - Lukić, Miodrag J.
AU  - Sezen, Meltem
AU  - Veljović, Đorđe
AU  - Mraković, Ana
PY  - 2017
UR  - https://dais.sanu.ac.rs/123456789/4611
AB  - The densification behavior of hydroxyapatite nanorods prepared by chemical precipitation method in open reactor conditions was investigated by application of different heating rates. The non-isothermal processing was performed with 2, 10, and 50 °C/min up to 1200 °C and yielded fully dense ceramics. The implementation of the higher heating rate provided grain size refinement from micrometer level for the slowest ramp, down to 250 nm in the case of processing with 50 °C/min, without any drawbacks regarding final density. The relative amount of retained structural hydroxyl groups in sintered ceramics was gradually increased with the heating rate. Furthermore, the qualitative level of optical translucency was increased with a higher heating rate which can be explained by the beneficial alignment of HAp nanorods during the fast heating rate processing, achieved microstructural refinement, and higher amount of structural hydroxyl groups.
PB  - Elsevier
T2  - Materials Letters
T1  - A facile route for hydroxyapatite densification with an increased heating rate
SP  - 12
EP  - 15
VL  - 207
DO  - 10.1016/j.matlet.2017.07.020
UR  - https://hdl.handle.net/21.15107/rcub_dais_4611
ER  - 

@article{
author = "Lukić, Miodrag J. and Sezen, Meltem and Veljović, Đorđe and Mraković, Ana",
year = "2017",
abstract = "The densification behavior of hydroxyapatite nanorods prepared by chemical precipitation method in open reactor conditions was investigated by application of different heating rates. The non-isothermal processing was performed with 2, 10, and 50 °C/min up to 1200 °C and yielded fully dense ceramics. The implementation of the higher heating rate provided grain size refinement from micrometer level for the slowest ramp, down to 250 nm in the case of processing with 50 °C/min, without any drawbacks regarding final density. The relative amount of retained structural hydroxyl groups in sintered ceramics was gradually increased with the heating rate. Furthermore, the qualitative level of optical translucency was increased with a higher heating rate which can be explained by the beneficial alignment of HAp nanorods during the fast heating rate processing, achieved microstructural refinement, and higher amount of structural hydroxyl groups.",
publisher = "Elsevier",
journal = "Materials Letters",
title = "A facile route for hydroxyapatite densification with an increased heating rate",
pages = "12-15",
volume = "207",
doi = "10.1016/j.matlet.2017.07.020",
url = "https://hdl.handle.net/21.15107/rcub_dais_4611"
}

Lukić, M. J., Sezen, M., Veljović, Đ.,& Mraković, A.. (2017). A facile route for hydroxyapatite densification with an increased heating rate. in Materials Letters
Elsevier., 207, 12-15.
https://doi.org/10.1016/j.matlet.2017.07.020
https://hdl.handle.net/21.15107/rcub_dais_4611

Lukić MJ, Sezen M, Veljović Đ, Mraković A. A facile route for hydroxyapatite densification with an increased heating rate. in Materials Letters. 2017;207:12-15.
doi:10.1016/j.matlet.2017.07.020
https://hdl.handle.net/21.15107/rcub_dais_4611 .

Lukić, Miodrag J., Sezen, Meltem, Veljović, Đorđe, Mraković, Ana, "A facile route for hydroxyapatite densification with an increased heating rate" in Materials Letters, 207 (2017):12-15,
https://doi.org/10.1016/j.matlet.2017.07.020 .,
https://hdl.handle.net/21.15107/rcub_dais_4611 .

TY  - JOUR
AU  - Lukić, Miodrag J.
AU  - Sezen, Meltem
AU  - Veljović, Đorđe
AU  - Mraković, Ana
PY  - 2017
UR  - https://dais.sanu.ac.rs/123456789/2336
AB  - The densification behavior of hydroxyapatite nanorods prepared by chemical precipitation method in open reactor conditions was investigated by application of different heating rates. The non-isothermal processing was performed with 2, 10, and 50 °C/min up to 1200 °C and yielded fully dense ceramics. The implementation of the higher heating rate provided grain size refinement from micrometer level for the slowest ramp, down to 250 nm in the case of processing with 50 °C/min, without any drawbacks regarding final density. The relative amount of retained structural hydroxyl groups in sintered ceramics was gradually increased with the heating rate. Furthermore, the qualitative level of optical translucency was increased with a higher heating rate which can be explained by the beneficial alignment of HAp nanorods during the fast heating rate processing, achieved microstructural refinement, and higher amount of structural hydroxyl groups.
PB  - Elsevier
T2  - Materials Letters
T1  - A facile route for hydroxyapatite densification with an increased heating rate
SP  - 12
EP  - 15
VL  - 207
DO  - 10.1016/j.matlet.2017.07.020
UR  - https://hdl.handle.net/21.15107/rcub_dais_2336
ER  - 

@article{
author = "Lukić, Miodrag J. and Sezen, Meltem and Veljović, Đorđe and Mraković, Ana",
year = "2017",
abstract = "The densification behavior of hydroxyapatite nanorods prepared by chemical precipitation method in open reactor conditions was investigated by application of different heating rates. The non-isothermal processing was performed with 2, 10, and 50 °C/min up to 1200 °C and yielded fully dense ceramics. The implementation of the higher heating rate provided grain size refinement from micrometer level for the slowest ramp, down to 250 nm in the case of processing with 50 °C/min, without any drawbacks regarding final density. The relative amount of retained structural hydroxyl groups in sintered ceramics was gradually increased with the heating rate. Furthermore, the qualitative level of optical translucency was increased with a higher heating rate which can be explained by the beneficial alignment of HAp nanorods during the fast heating rate processing, achieved microstructural refinement, and higher amount of structural hydroxyl groups.",
publisher = "Elsevier",
journal = "Materials Letters",
title = "A facile route for hydroxyapatite densification with an increased heating rate",
pages = "12-15",
volume = "207",
doi = "10.1016/j.matlet.2017.07.020",
url = "https://hdl.handle.net/21.15107/rcub_dais_2336"
}

Lukić, M. J., Sezen, M., Veljović, Đ.,& Mraković, A.. (2017). A facile route for hydroxyapatite densification with an increased heating rate. in Materials Letters
Elsevier., 207, 12-15.
https://doi.org/10.1016/j.matlet.2017.07.020
https://hdl.handle.net/21.15107/rcub_dais_2336

Lukić MJ, Sezen M, Veljović Đ, Mraković A. A facile route for hydroxyapatite densification with an increased heating rate. in Materials Letters. 2017;207:12-15.
doi:10.1016/j.matlet.2017.07.020
https://hdl.handle.net/21.15107/rcub_dais_2336 .

Lukić, Miodrag J., Sezen, Meltem, Veljović, Đorđe, Mraković, Ana, "A facile route for hydroxyapatite densification with an increased heating rate" in Materials Letters, 207 (2017):12-15,
https://doi.org/10.1016/j.matlet.2017.07.020 .,
https://hdl.handle.net/21.15107/rcub_dais_2336 .